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CASE REPORTJ Neurosurg Spine 26:319–324, 2017
Central nervous system (CNS) germ cell tumor is a rare pathology that accounts for approximately 2%–3% of CNS tumors in Japan and 0.3%–0.6% in
Western countries.10,15 Most CNS germ cell tumors occur in the suprasellar or pineal region, followed by the basal ganglia, and germ cell tumors originating in the spinal cord are extremely rare. While a standard therapeutic strategy for intracranial germ cell tumor has been evaluated to some extent, recommended treatment regimens for primary spi-nal germ cell tumor are less developed due to the rarity of this entity. In particular, primary spinal nongermino-matous germ cell tumor (NGGCT) has been reported in only a small number of cases,2,5,9,20,21 including 2 cases of spinal yolk sac tumor that had poor outcomes despite in-tensive chemoradiotherapy.5,9 Effective treatment protocols
against spinal NGGCT thus need to be developed. Here, we present a case of spinal mixed germ cell tumor comprising yolk sac tumor and mature teratoma. Successful treatment was achieved using whole-craniospinal irradiation and lo-cal spinal irradiation followed by ifosfamide, cisplatin, and etoposide (ICE) chemotherapy, based on a protocol widely used in Japan for the subset of intracranial germ cell tu-mors categorized as having a poor prognosis.13,14
Case ReportHistory and Examination
A 24-year-old woman who had noticed back pain after physical exercise a month earlier presented with progres-sive numbness and weakness of the lower extremities. She
ABBREVIATIONS AFP = alpha-fetoprotein; BEP = bleomycin, etoposide, and cisplatin; CE = carboplatin and etoposide; CNS = central nervous system; ICE = ifosfamide, cisplatin, and etoposide; JPBTSG = Japanese Pediatric Brain Tumor Study Group; NGGCT = nongerminomatous germ cell tumor; OS = overall survival rate; PE = cisplatin and etoposide; PVB = cisplatin, vinblastine, and bleomycin; STGC = syncytiotrophoblastic giant cell. SUBMITTED April 26, 2016. ACCEPTED August 31, 2016.INCLUDE WHEN CITING Published online December 2, 2016; DOI: 10.3171/2016.8.SPINE16465.* Drs. A. Mukasa, S. Yanagisawa, and K. Saito contributed equally to this work.
Successful treatment of mixed yolk sac tumor and mature teratoma in the spinal cord: case report*Akitake Mukasa, MD, PhD,1 Shunsuke Yanagisawa, MD,1 Kuniaki Saito, MD,1 Shota Tanaka, MD,1 Keisuke Takai, MD, PhD,1,5 Junji Shibahara, MD, PhD,2 Masachika Ikegami, MD,3 Yusuke Nakao, MD,3,6 Katsushi Takeshita, MD, PhD,3,7 Masao Matsutani, MD, PhD,4 and Nobuhito Saito, MD, PhD1
Departments of 1Neurosurgery, 2Pathology, and 3Orthopaedic Surgery and Spinal Surgery, The University of Tokyo Hospital, Tokyo; and 4Department of Neuro-Oncology/Neurosurgery, Saitama Medical University International Medical Center, Hidaka, Japan
Primary spinal germ cell tumors are rare, and spinal nongerminomatous germ cell tumors represent an even rarer subset for which no standard therapy has been established. The authors report the case of a 24-year-old woman with multifo-cal primary spinal germ cell tumors scattered from T-12 to L-5 that consisted of yolk sac tumor and mature teratoma. After diagnostic partial resection, the patient was treated with 30 Gy of craniospinal irradiation and 30 Gy of local spinal irradiation, followed by 8 courses of chemotherapy based on ifosfamide, cisplatin, and etoposide (ICE). Salvage surgery was also performed for residual mature teratoma components after the third course of ICE chemotherapy. Chemo-therapy was continued after the operation, but ifosfamide was entirely eliminated from the ICE regimen because severe myelosuppression was observed after previous courses. The patient remains recurrence free as of more than 5 years after the completion of chemotherapy. This case suggests that this treatment strategy is an effective option for primary spinal yolk sac tumor.https://thejns.org/doi/abs/10.3171/2016.8.SPINE16465KEY WORDS germ cell tumor; spinal cord; yolk sac tumor; teratoma; radiotherapy; chemotherapy; oncology
©AANS, 2017 J Neurosurg Spine Volume 26 • March 2017 319
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could not walk but could still move her right leg. Neu-rological examination revealed severe paraparesis of the lower limbs (paralysis of the left lower leg and severe weakness of the right lower leg), sensory disturbance be-low T-10, and bladder-bowel dysfunction. Both knee-jerk and Achilles tendon reflexes were deteriorated.
Magnetic resonance imaging (MRI) of the spinal cord demonstrated mass lesions 30 mm in diameter at T-10 and L2–3, as well as several smaller lesions between T-12 and L-5 (Fig. 1). Cranial MRI revealed no lesions in the brain. Imaging with 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) showed elevated maximum stan-dardized uptake values (SUVmax) of 3.2 at the T-10 lesion and 4.2 at the L2–3 lesion.
Operation and Pathological FindingsBecause the T-10 lesion was suspected of being re-
sponsible for the progressive paraparesis, emergent de-compressive laminectomy of T9–10 and partial resection of the tumor located at T-10 were performed. Although decompressive laminectomy was performed, the patient’s symptoms progressed further, and her lower legs were completely paralyzed during the postoperative period. Histopathological examination indicated a yolk sac tumor (Fig. 2), and blood tests showed elevated levels of alpha-fetoprotein (AFP) (2182 ng/ml) and normal levels of beta–human chorionic gonadotropin (b-HCG).
FIG. 1. Gadolinium-enhanced T1-weighted (A–C, E, and F) and T2-weighted (D) MR images showing spinal germ cell tumors. Mass lesions (arrowheads) approximately 30 mm in diameter can be seen at T-10 (A, sagittal image; B, axial image) and L2–3 (A and C [axial image]). The sagittal T2-weighted image (D) shows peritumoral edema within the spinal cord. The sagittal images in panels E and F show several smaller lesions (arrows) between T-12 and L-5.
FIG. 2. Surgical specimens of yolk sac tumor obtained at the T-10 level. A and B: H & E staining reveals primitive-appearing cells in a reticular-microcystic pattern. C and D: Immunohistochemical staining reveals the presence of SALL4 (C) and AFP (D). Bar = 500 μm (A), 50 μm (B and D), and 100 μm (C).
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Treatment of spinal nongerminomatous germ cell tumor
J Neurosurg Spine Volume 26 • March 2017 321
Postoperative CourseBecause a standard chemoradiotherapy regimen is
lacking for spinal germ cell tumors, the patient was treated with a slight modification of the protocol proposed by the Japanese Pediatric Brain Tumor Study Group (JPBTSG) for intracranial NGGCT with poor prognosis.13,14 Briefly, the patient received 30 Gy of whole-craniospinal irradia-tion delivered in 17 fractions, followed by local boost ir-radiation at the spinal lesions consisting of 30 Gy deliv-ered in 17 fractions. Eight cycles of ICE chemotherapy were then administered. All radiotherapy was conducted prior to chemotherapy, because the tumors were growing rapidly and radiotherapy was thought to be more reliable than chemotherapy for the immediate control of tumor masses. The ICE chemotherapy regimen comprised 900 mg/m2 of ifosfamide (Days 1–5), 20 mg/m2 of cisplatin (Days 1–5), and 60 mg/m2 of etoposide (Days 1–5). Due to severe myelosuppression appearing during the course of chemotherapy, dose reduction was conducted following the method described by Sawamura et al.;17 a reduced dose of etoposide and ifosfamide was administered for the sec-ond and third courses, and ifosfamide was therefore not used during the fourth to eighth courses.
The patient’s serum AFP level rapidly decreased to less than 100 ng/ml toward the end of radiotherapy, and it fell further to within the normal range after the first cycle of ICE chemotherapy, which was carried out right after the radiotherapy. In accordance with the decrease in serum AFP level, spinal MRI demonstrated marked shrinkage of the enhanced lesions, but remnant tumor was suspected at L-2 (Fig. 3A) after the third course of ICE chemotherapy. Salvage surgery was therefore performed. One nerve root was found to be completely involved with the remnant tu-mor, and both the residual tumor and the nerve root were
totally removed (Fig. 3B). The histopathological diagnosis of this remnant was mature teratoma, with no evidence of residual yolk sac tumor (Fig. 3C), suggesting that the chemoradiotherapy had successfully eradicated the yolk sac tumor component.
Five courses of adjuvant chemotherapy, consisting of cisplatin and a 75%–100% dose of etoposide, were ad-ministered after the salvage surgery; the chemotherapy was completed 18 months after the initial operation. MRI (Fig. 3D) and measurement of AFP levels (3 ng/ml) showed no evidence of recurrence at more than 5 years after completion of chemotherapy. The patient still has the severe paraplegia of the lower limbs, sensory dis-turbance below T-10, and bladder-bowel dysfunction that were present before treatment, but she is otherwise healthy and did not incur any additional deficit due to the nerve root resection.
DiscussionHere, we have described a case we encountered of
primary malignant spinal germ cell tumor, which is ex-tremely rare. The patient was successfully treated with whole-craniospinal and local irradiation followed by ICE chemotherapy and salvage surgery. Our experience and review of the literature suggest that this treatment strategy might be effective for malignant spinal germ cell tumor, although further evaluation of the treatment protocol is warranted.
Primary Spinal Germ Cell TumorsPrimary spinal germ cell tumors are rare. To date, in-
cluding our case of mixed germ cell tumor, 38 cases of primary spinal germ cell tumor have been reported—in-
FIG. 3. A: Sagittal gadolinium-enhanced T1-weighted image of the spinal cord obtained after the third course of ICE chemo-therapy, revealing residual tumor at L-2 (arrowhead). B: Intraoperative photograph showing involvement of the nerve root by the tumor. C: H & E–stained specimen demonstrating mature cystic teratoma. The cysts frequently contain keratinized material. The cyst with keratinized material is accompanied by foreign body reaction (right) and the other cyst is lined by ciliated epithelium (left). Bar = 100 μm. D: Sagittal gadolinium-enhanced T1-weighted image of the spinal cord obtained approximately 67 months after completion of chemotherapy. No evidence of tumor recurrence was found.
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A. Mukasa et al.
J Neurosurg Spine Volume 26 • March 2017322
cluding 27 cases of pure germinoma (71%), 5 cases of ger-minoma with syncytiotrophoblastic giant cells (STGCs) (13%), 3 cases of mixed germ cell tumor (8%), 2 cases of yolk sac tumor (5%), and 1 case of embryonal carcinoma (3%).1,2,5,7,9,11,12,16,19–21 The proportion of NGGCTs among primary spinal germ cell tumors appears slightly lower than that among intracranial germ cell tumors.15 Among these 38 cases, 25 (66%) involved Japanese patients and 19 (50%) involved male patients. Whereas intracranial germ cell tumors seem more frequent in males, the sex ratio ap-pears about equal for primary spinal germ cell tumors. The mean age at diagnosis of primary spinal germ cell tumors is 25 years (range 1.4–45 years). Primary spinal germ cell tumors occur most frequently in the thoracic spinal cord and least frequently in the cervical region. Of the 38 patients, 6 (16%) were reported to experience recur-rence; those recurrences involved germinoma in 2 cases, germinoma with STGC in 1 case, yolk sac tumor in 2 cases, and embryonal carcinoma in 1 case. The recurrence rate of primary spinal NGGCT (3 of 6, 50%) is signifi-cantly higher than that of primary spinal germinoma (3 of 32, 9.4%; Fisher’s exact test, p < 0.05).
RadiotherapyExcluding 1 case of germinoma with STGC in which
the postoperative treatment was unclear, 36 primary tu-mors were treated with radiotherapy. Local-field radio-therapy was performed in most cases (34 of 36, 94%), with 15 patients (41%) receiving whole-spine irradiation and 10 of these 15 patients also treated with whole-brain irradia-tion. Among the 19 patients with germinoma with or with-out STGC who did not receive whole-spine irradiation, 3 patients (16%) suffered recurrence. CNS dissemination was evident in 2 of these 3 cases. In contrast, no recur-rence was seen in the 12 patients with germinoma who received whole-spine irradiation. The 3 patients who suf-fered recurrence had not been treated with systemic che-motherapy, suggesting that treatment with local radiation alone is insufficient to control even germinoma.
ChemotherapyAmong the 37 cases of primary tumors whose postop-
erative treatment was reported, chemotherapy was per-formed in 20 (54%). This chemotherapy consisted of CE (carboplatin and etoposide) in 7 cases, ICE in 6 cases, BEP (bleomycin, etoposide, and cisplatin) in 3 cases, PE (cisplatin and etoposide) in 2 cases, PVB (cisplatin, vin-blastine, and bleomycin) in 2 cases, and methotrexate and etoposide in 1 case. (In 1 case, the patient was treated with both CE and ICE.) The patients with NGGCT were treated with various chemotherapeutic regimens (Table 1). Among the patients with primary spinal germinoma, 2 of the 17 cases treated without chemotherapy had tumor recurrence, whereas only 1 of the 14 patients treated with chemotherapy showed recurrence. CE chemotherapy was used for 6 cases of germinoma, and all 6 patients reportedly remained free of recurrence, although CE chemotherapy used for 1 NGGCT case was not effective. All 6 patients treated with ICE chemotherapy likewise showed no recurrence over a reported follow-up of 22–88 months. These 6 cases treated with ICE chemotherapy TA
BLE
1. Su
mm
ary o
f cas
es o
f prim
ary s
pina
l NGG
CT re
porte
d in
the l
itera
ture
Auth
ors &
Yea
rAg
e, Se
xPa
tholo
gyTu
mor L
ocati
onSu
rger
yRa
dioth
erap
y (Gy
)Ch
emoth
erap
yTr
eatm
ent
Resp
onse
*OS
(m
os)†
Recu
rrenc
eSt
atus
CSI
Loca
l
Kuris
aka e
t al.,
1998
17 m
os, F
YST
T2–7
STR
025
PVB ×
2 cyc
lesCR
7Lo
cal
Dead
Kan e
t al.,
2006
25 yr
s, F
YST
L1–3
STR
040
PE ×
4 cy
cles‡
CR22
Mult
iple§
Dead
Taka
hash
i et a
l., 20
0622
yrs,
FM
X1L1
–2ST
R27
(spin
e), 3
0.6 (b
rain)
30.6
ICE
(carb
o) ×
2 cy
cles,
ICE
× 1 c
ycle
CR22
None
Alive
Bisw
as et
al., 2
009
28 yr
s, M
ECL2
–4GT
R0
0¶BE
P ×
4 cyc
lesCR
11M
ultipl
e§De
adYa
mamo
to et
al., 2
013
42 yr
s, F
MX2
L1–3
GTR
2421
CE ×
2 cy
cles,
ICE ×
4 cyc
lesCR
**26
None
Alive
Pres
ent c
ase
24 yr
s, F
MX3
T-10,
L2–3
, T12
–L5
Bx &
salva
ge††
3030
ICE ×
8 cyc
lesCR
††88
None
Alive
Bx =
biop
sy; C
R =
comp
lete r
emiss
ion; C
SI =
cran
iospin
al irr
adiat
ion; E
C =
embr
yona
l car
cinom
a; GT
R =
gros
s-tot
al re
secti
on; I
CE (c
arbo
) = ifo
sfami
de, c
arbo
platin
, and
etop
oside
; MX1
= ge
rmino
ma +
chor
iocar
cinom
a; M
X2 =
germ
inoma
+ im
matu
re te
rato
ma; M
X3 =
yolk
sac t
umor
+ m
atur
e ter
atoma
; STR
= su
btota
l rese
ction
; YST
= yo
lk sa
c tum
or.
* Re
spon
se to
initia
l set
of th
erap
ies de
scrib
ed in
this
table
.†
Indic
ates o
vera
ll sur
vival
as of
this
writin
g.‡
Afte
r 4 cy
cles o
f PE
chem
othe
rapy
and r
adiot
hera
py, 4
addit
ional
cycle
s of c
onso
lidat
ion ch
emot
hera
py w
ere p
erfo
rmed
with
carb
oplat
in, vi
nblas
tine,
etopo
side,
and b
leomy
cin.
§ Lo
cal re
curre
nce w
ith di
ssem
inatio
n and
/or m
etas
tasis
was
susp
ecte
d.¶
Patie
nt wa
s tre
ated w
ith 2
0 Gy o
f loca
l rad
iothe
rapy
afte
r tum
or re
curre
nce.
** Po
stope
rativ
ely, 2
cycle
s of C
E ch
emot
hera
py w
ere a
dmini
stere
d. Si
nce t
umor
prog
ress
ion w
as o
bser
ved a
fter C
E ch
emot
hera
py, t
he tr
eatm
ent p
roto
col w
as al
tere
d and
4 cy
cles o
f ICE
chem
othe
rapy
and i
rradia
tion
were
admi
niste
red.
†† T
he yo
lk sa
c com
pone
nt of
MX3
show
ed a
comp
lete r
espo
nse,
but s
ince t
he m
atur
e ter
atoma
comp
onen
t did
not r
espo
nd to
chem
orad
iothe
rapy
, salv
age s
urge
ry w
as p
erfo
rmed
.
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Treatment of spinal nongerminomatous germ cell tumor
J Neurosurg Spine Volume 26 • March 2017 323
include 2 pure germinomas, 1 germinoma with STGC, and 3 mixed germ cell tumors (germinoma + choriocar-cinoma, germinoma + immature teratoma, and yolk sac tumor + mature teratoma). Such findings suggest that ICE chemotherapy may be effective against primary spinal germ cell tumors, including NGGCT.
Primary Spinal NGGCTPrimary spinal NGGCT is extremely rare, and only 6
cases have been reported, including the present case. The 6 cases include 2 yolk sac tumors,5,9 1 embryonal carcino-ma,2 and 3 mixed germ cell tumors (germinoma + chorio-carcinoma, germinoma + immature teratoma, and mature teratoma + yolk sac tumor)18,21 (Table 1). Of these 6 cases, 5 would be categorized into the poor prognosis group ac-cording to the classification of the JPBTSG.13,14 Only 1 of these 6 patients was male, and the mean age at diagnosis was 24 years. The 3 patients with mixed germ cell tumors were treated with whole-craniospinal irradiation and local irradiation combined with ICE chemotherapy, and none showed any recurrence during follow-up (range of overall survival 22–88 months). In contrast, the 3 other patients with spinal NGGCT (2 yolk sac tumors and 1 embryonal carcinoma) were treated with chemotherapy (PVB, PE, or BEP) with or without local irradiation, and all 3 showed recurrence and died within 22 months (mean overall sur-vival 13 months). These small case series suggest that whole-craniospinal irradiation and local spinal irradiation combined with ICE chemotherapy as we performed rep-resents a promising treatment protocol for primary spinal NGGCT.
Although the number of reported cases of spinal germ cell tumor is quite limited, the results of clinical studies of intracranial germ cell tumor suggest a recommended pro-tocol for the treatment of patients with malignant germ cell tumors. Studies of intensive chemotherapy for malignant intracranial germ cell tumor have shown that NGGCT is highly responsive to chemotherapy and demonstrate a 5-year overall survival rate (OS) of nearly 60%–75% for patients treated with chemotherapy without irradiation at initial therapy (most of the patients were subsequently treated with radiotherapy); nevertheless, chemotherapy alone results in a higher relapse rate than chemotherapy combined with radiotherapy, indicating that irradiation should be included in treatment regimens.4,6 Bromberg et al.3 reviewed the recent literature for intracranial germ cell tumor and reported that radiotherapy combined with platinum-based chemotherapy was effective and should represent the current standard for intracranial NGGCT. Neoadjuvant chemoradiotherapy is also an effective strat-egy, as Kochi et al.8 treated 11 patients with NGGCT using neoadjuvant chemotherapy with platinum-based agents (CE, PE, or ICE) and radiotherapy and reported that the 5-year progression-free survival rate and 5-year OS were both 90.9%. Taking these results together, ap-plication of combined radiation and ICE chemotherapy for spinal germ cell tumor appears reasonable. Of note, spinal NGGCT requires treatment with whole-spine irradiation, which often causes severe myelosuppression and would thus make the subsequent intensive chemotherapy more difficult. The protocol for intracranial NGGCT may there-
fore require some modifications to achieve the optimal regimen for spinal NGGCT. In this regard, whether irradi-ation should be performed before or after several courses of chemotherapy is a matter for discussion. We decided to use radiotherapy prior to chemotherapy in the present case, as this therapy is more reliable for controlling rapidly growing tumors. Due to the severe myelosuppression that resulted, we were obliged to reduce the dose of ICE che-motherapy, and ifosfamide was entirely eliminated after the third course of ICE chemotherapy. Initial irradiation thus carries a risk of requiring a reduction in the strength of subsequent chemotherapy. The timing of radiation may therefore warrant further evaluation.
References 1. Ashraf S: Intramedullary cervical spine germinoma: a case
report. Med J Malaysia 67:207–209, 2012 2. Biswas A, Puri T, Goyal S, Gupta R, Eesa M, Julka PK, et
al: Spinal intradural primary germ cell tumour—review of literature and case report. Acta Neurochir (Wien) 151:277–284, 2009
3. Bromberg JE, Baumert BG, de Vos F, Gijtenbeek JM, Kurt E, Westermann AM, et al: Primary intracranial germ-cell tumors in adults: a practical review. J Neurooncol 113:175–183, 2013
4. da Silva NS, Cappellano AM, Diez B, Cavalheiro S, Gardner S, Wisoff J, et al: Primary chemotherapy for intracranial germ cell tumors: results of the third international CNS germ cell tumor study. Pediatr Blood Cancer 54:377–383, 2010
5. Kan P, Gottfried ON, Blumenthal DT, Liu JK, Salzman KL, Townsend J, et al: Primary spinal yolk sac tumor with brain metastasis: case report and review of the literature. J Neurooncol 78:249–253, 2006
6. Kellie SJ, Boyce H, Dunkel IJ, Diez B, Rosenblum M, Brualdi L, et al: Primary chemotherapy for intracranial nongerminomatous germ cell tumors: results of the second international CNS germ cell study group protocol. J Clin Oncol 22:846–853, 2004
7. Kinoshita Y, Akatsuka K, Ohtake M, Kamitani H, Watanabe T: Primary intramedullary spinal cord germinoma. Neurol Med Chir (Tokyo) 50:592–594, 2010
8. Kochi M, Itoyama Y, Shiraishi S, Kitamura I, Marubayashi T, Ushio Y: Successful treatment of intracranial nongerminomatous malignant germ cell tumors by administering neoadjuvant chemotherapy and radiotherapy before excision of residual tumors. J Neurosurg 99:106–114, 2003
9. Kurisaka M, Moriki A, Mori K, Sonobe H: Primary yolk sac tumor in the spinal cord. Childs Nerv Syst 14:653–657, 1998
10. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK (eds): WHO Classification of Tumours of the Central Nervous System, ed 4. Lyon: International Agency for Research on Cancer, 2007
11. Loya JJ, Jung H, Temmins C, Cho N, Singh H: Primary spinal germ cell tumors: a case analysis and review of treatment paradigms. Case Rep Med 2013:798358, 2013
12. Madhukar M, Maller VG, Choudhary AK, Iantosca MR, Specht CS, Dias MS: Primary intramedullary spinal cord germinoma. J Neurosurg Pediatr 11:605–609, 2013
13. Matsutani M: Clinical management of primary central nervous system germ cell tumors. Semin Oncol 31:676–683, 2004
14. Matsutani M: Combined chemotherapy and radiation therapy for CNS germ cell tumors—the Japanese experience. J Neurooncol 54:311–316, 2001
15. McCarthy BJ, Shibui S, Kayama T, Miyaoka E, Narita Y,
Unauthenticated | Downloaded 06/01/20 09:24 PM UTC
A. Mukasa et al.
J Neurosurg Spine Volume 26 • March 2017324
Murakami M, et al: Primary CNS germ cell tumors in Japan and the United States: an analysis of 4 tumor registries. Neuro Oncol 14:1194–1200, 2012
16. Mehta VA, Kretzer RM, Orr B, Jallo GI: Primary intramedullary spinal germ cell tumors. World Neurosurg 76:478.e1–478.e6, 2011
17. Sawamura Y, Ikeda J, Ishii N, Kato T, Tada M, Abe H, et al: Combined irradiation and chemotherapy using ifosfamide, cisplatin, and etoposide for children with medulloblastoma/posterior fossa primitive neuroectodermal tumor—results of a pilot study. Neurol Med Chir (Tokyo) 36:632–638, 1996
18. Takahashi M, Koyama H, Matsubara T, Murata H, Miura K, Nagano A: Mixed germinoma and choriocarcinoma in the intramedullary spinal cord: case report and review of the literature. J Neurooncol 76:71–75, 2006
19. Wang R, Fan X, Zhang B: A rare case of multifocal intramedullary germinoma in cervical spinal cord. Spinal Cord 52 (Suppl 1):S19–S22, 2014
20. Yamagata T, Takami T, Tsuyuguchi N, Goto T, Wakasa K, Ohata K: Primary intramedullary spinal cord germinoma: diagnostic challenge and treatment strategy. Neurol Med Chir (Tokyo) 49:128–133, 2009
21. Yamamoto J, Takahashi M, Nakano Y, Saito T, Kitagawa T, Ueta K, et al: Intratumoral hemorrhage because of primary spinal mixed germ cell tumor presenting with atypical radiological features in an adult. Spine J 13:e31–e38, 2013
DisclosuresThe authors report no conflict of interest concerning the materi-als or methods used in this study or the findings specified in this paper.
Author ContributionsConception and design: Mukasa, Yanagisawa, K Saito. Acquisi-tion of data: Mukasa, Yanagisawa, K Saito, Tanaka, Takai, Shiba-hara, Ikegami, Nakao, Takeshita. Analysis and interpretation of data: Mukasa, Yanagisawa, K Saito, Tanaka, Takai, Shibahara, Takeshita, Matsutani. Drafting the article: Yanagisawa, K Saito, Shibahara. Critically revising the article: Mukasa, Yanagisawa, K Saito, Tanaka, Takai, Shibahara, Takeshita, Matsutani, N Saito. Reviewed submitted version of manuscript: all authors. Approved the final version of the manuscript on behalf of all authors: Muka-sa. Administrative/technical/material support: Takai, Shibahara, Ikegami, Nakao, Takeshita, Matsutani, N Saito. Study supervi-sion: Matsutani, N Saito. Involved in patient’s treatment: Mukasa, K Saito, Tanaka, Shibahara. Involved in patient’s surgical treat-ment: Mukasa, Takai, Ikegami, Nakao, Takeshita.
CorrespondenceAkitake Mukasa, Department of Neurosurgery, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. email: [email protected].
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