Stem Cell Slide

7/23/2019 Stem Cell Slide

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Stemcell Update

Indra Bachtiar



Stem Cell News





Stem Cell Cream?



Controversy the Use of Stem Cells



Stem Cells?





UCB/UC-Wharton jelly

Bone Marrow

Peripheral Blood

Adiposetisse

Surgical Waste

Adlt Stem Cells



Indced Pluripotent Stemcell (iPS) Cells

A new PERSPECTIVE:

• !o o"tain plripotent stem

cells #rom adlt tisses$%"ro"lasts&

And a new POTENTIAL:

• !o o"tain #llydi'erentiated cells #rom

iPSCs #or re(enerative andreprodctive medicine

Yamanaka S. Cell 2!" #$%&#$

r 'on . *urdon + Yamanaka S (2#2)



Capa"ilities of Stem Cells

Stem celltype Description Examples

TotipotentEach cell can develop

into a new individual

Cells from early(1-3 days)

embryos

PluripotentCells can form any(over !!) cell types

"ome cells ofblastocyst (# to1$ days)

%ultipotentCells differentiated&but can form a numberof other tissues

'etal tissue& cordblood& and adultstem cells



!otipotent Cells

• Can become

*AN Y*

• kind of cell• Ex-

• Zygote ( cell!

• "or#la ($ cells!• % & days



)istory o# Cloning$!otipotent&

• !!1 Cat cloned 22 , -aits cloned 2$ , /ule cloned

'CC Carbon Copy

20 , ull serial1cloned 2 , 3og cloned



Plripotent Cells

• Can become )

• "ost

• kinds of cell• Ex-

• lastocyst+s

inner cell mass

(,C"!

• & days



Mltipotent Cells

• Can become )

• Limited

• kinds of cells.• /s#ally become

• Cell type t0at

matc0es tiss#e

type 10ere stemcell is



!ypes o# Stem Cells

• *m"ryonic stem cells are capa"leo# developin( into all the cell typeso# the "ody+

• Adlt stem cells are less versatile

and more di,clt to isolate andpri#y+



*m"ryonic Stem Cells



Sorces o# *m"ryonic Stem

Cells

1 E*cess fertili+ed e,,s from .' (in-vitro

fertili+ation) clinics

Therapeutic clonin, (somatic cell nuclear

transfer)



4ens oftousands of

fro5en emr6osare routinel6destro6ed wencouples 7nis

teir treatment. 4ese surplusemr6os can eused to producestem cells.

-egenerati8emedical researcaims to de8eloptese cells into

new9 ealt6



Somatic Cell.clear !rans#er

4e nucleus of adonated egg isremo8ed and replacedwit te nucleus of amature9 :somatic cell:(a skin cell9 for

e;ample).

No sperm is in8ol8edin tis process9 and noemr6o is created toe implanted in awoman<s wom.

4e resulting stemcells can potentiall6de8elop into

speciali5ed cells tatare useful for treating



SCAN – Stem Cell Action Network

!he *thical Prolem

"tem cells are ta/en from a human blastocyst&which is then destroyed This amounts to 0murder

• There is a ris/ of commercial e*ploitation of thehuman participants

• E"C will lead to reproductive clonin,



Somatic/Adlt Stem Cell



Adlt Stem Cells

)ematopoietic stem cells , formsall lood cell t6pes

Mesenchymal stem cells ,tatgenerates one9 cartilage9 fat and

7rous connecti8e tissue



)ematopoietic Stem Cells (UC9 loodPerifer)



Mesenchymal Stem Cells (/SC)



Mltypotent 1 self renewal and

wide di=erentiation into multiplelineages

ow immno(enicity (transplanted

/SC are not re>ected 6 oterpersons)



pa"ilities of /esenc6mal Stem Cells



0ecent Sorces o# /SC



Bone /arrow



Adipose tissue

Surgical Waste



Um"ilical Cord



)ow to Identi#y Stem Cells(/esenc6mal Stem Cells)



1ality !estin(

lowc6tometr6 @nal6sis



Differentiation into:

- Chondrocytes Alcian blue staining

- Osteocytes Toluine blue staining

- Adipocytes Oil Red O staining

Condroc6tes @dipoc6tesAsteoc6tes

2I33*0*.!B@4BAN



46pes of Stem Cell !ransplantation







)ow to 2eliver of Stem Cell





-epresentati8e wole1od6 images demonstrating teiodistriution of //Cs laeled wit !!m 4c9 2 and 20 (n D E foreac group) after intra8enous or intra1arterial in>ection in iscemicrats. 4e intra1arterial group ad signi7cant acti8it6 in te ead at2 . 4e remaining acti8it6 could e seen in te lungs9 li8er9

kidne6s and ladder in all groups at 2 9 and in te li8er andkidne s at 20 .



rain distriution of //Cs laeled wit !!m 4c9 2 (@) and 20 () after intra8enous orintra1arterial in>ection in non1iscemic (n D 0) and iscemic (n D E) rats 6 gamma1well

counting 4racer acti8it6 is e;pressed as te percentage of in>ected dose per gram of organ (FB3Gg) H p I . paired t1test.

MSC )omin( in Ischemic Area



!he *'ect o# A(e on Stemcell Capacity







A(e-0elated @41/SC 3i=erentiation



2i'erent Sorce Jas 3i=erent Capacit6



Pro in4amatory C6tokines



Pro-in4amatory C6tokines

@ pro-in4ammatory cyto5ine is a c6tokine wic promotes

s6stemic inKammation

Concentration of Cemokines



Concentration of Cemokines

Cemokines are speci7c c6tokines tat functions6 attracting cells to sites of infectionGinKammation.

6 h 3 C i



6rowth 3actor Concentration



Concentration of An(io(enic 3actors



Secreted @mounts of*7tracelllar

Matri7 Components



Stemcell Mechanism and Action



Stem Cell Action In 2ia"etic 2esease



Stemcell Adhesion 4o Pancreatic Bslets

@ttaced /SCs are clearl6 8isile as 6ellow spots (arrows) on greenpancreatic islets after # week of culture (a) as well as after 0 weeks(). Bn green& calcein stained pancreatic islets. Bn red& 3iB stained/SCs. ar # mm Scuteri et al. 2#09 PLoS ANM !(#)



Inslin 0elease @fter *lucose Stimulation

Mac week (up to 0 weeks of culture) pancreatic islets cultured alone or directl6 co1culturedwit /SCs9 and /SCs cultured alone were e;posed to di=erent glucose concentrations in teculture medium (2 m/ and #9E% m/ *lucose)9 and te insulin release after eac cangewas measured 6 an MLBS@ assa6 speci7c for tis ormone

Scuteri et al. 2#09 PLoS ANM !(#)



Copyri,ht 2 merican 4eart ssociation& nc ll ri,hts reserved

Stem Cell Action In Cardiac 0epair

/se of 2ario#s 3ypes of Stem Cell t0erapies in patients 1it0



/se of 2ario#s 3ypes of Stem Cell t0erapies in patients 1it0

Cardio4asc#lar Disease.


S f St C ll / d f C di 5 i



So#rces of Stem Cells /sed for Cardiac 5epair


6 t ti l " 0 i f A ti f St C ll



6otential "ec0anisms of Action of Stem Cells.


Paracrine/Atocrine Mechanisms In Stem Cell



Paracrine/Atocrine Mechanisms In Stem CellSi(nalin(

"assimiliano 7necc0i et al. Circ#lation 5esearc0.

899:;9<=89>-8?


Mechanisms Bn8ol8ed Bn Neo8asculari5ation



Mechanisms Bn8ol8ed Bn Neo8asculari5ation+




Clinical @pplications

6eo(raphic ocation o# 898: Stem



6eo( ap c oca o o : S eCell

Clinical 4rials *loall6

0e(en+ Med+ $;<=8& :$=&;9>:



0e(en+ Med+ $;<=8& :$=&;9>:



St ll Cli i l 4 i l i B d i



Stemcell Clinical 4rial in Bndonesia



Brn

Cr6opreser8ed UC/NC

3eep partial tickness urn

-e1epiteliali5ationprocess

Stem Cellapplication

il



)eart 3ailre

• Net loss of contractile tissue in patientwit coronar6 arter6 disease

• /a>or cause of eart failure

-e8asculari5ation (enance loodPerfusion)

0e(enerate myocardim cells

)i(h 0ateMortality

Mndotelial loss (35au et al. 2)

Low numer of circulating Mndotelial Progenitor Cells(MPCs) in periperal lood (arer + @rispe. 2E)

C i i l i " B i



Critical im" Bscemia

Peripheral Arterial2iseasesnarrowin( and hardenin( o# thearteries that spply the le(sand #eet

!herapetic

an(io(enesis(stem Cell)Sources stem cell& one marrow (4ateisi Yu6ama et al.

22) Periperal lood (Bsida et al. 2)

6an(rene tissedeath ornecessitatin(amptation

IMP0@*2

0mah Sa5it)asan



CI

2 patients a8e een treated wit tismetod

Be#ore stem celltherapy

8< days a#ter stem celltherapy

-esult patient 2

)asanSadi5in



month a#terimplantation

M0I "e#oreimplantation

@steoartritis



.on-nion oneracture

before beforebeforeunion at

week 8

union at

week 4

union at

week 4

Adipose Stem Cell



Adipose-Stem Cellfor Skin -e>u8enation



Breast @ugmentation



Breast @ugmentation

ey Considerations



yor uture Stem Cell -esearc and

@pplication



"Imagination is moreimportant than knowledge ."

@lert Minstein (#0 /arc #O%! , #O @pril #!)

4ank You

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