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Supporting Information
Identification of microalgae by laser desorption/ionization mass spectrometry coupled with multiple nanomatrices
Lung-Hsiang Peng,a Binesh Unnikrishnan,a Chi-Yu Shih,b Tung-Ming Hsiung,a Jeng Chang,bcd Pang-Hung Hsu,a Tai-Chia Chiu,*e and Chih-Ching Huang*acf
aDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; bInstitute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan; cCenter of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan; dInstitute of Marine Environmental Chemistry and Ecology, National Taiwan Ocean University, Keelung, Taiwan; eDepartment of Applied Science, National Taitung University, Taitung, Taiwan; fSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
Correspondence: Tai-Chia Chiu, Department of Applied Science, National Taitung University, 369, Sec. 2, University Rd, Taitung 95092, Taiwan; Tel.: +886-89-517990; Fax: +886-89-518108; E-mail: [email protected]; Chih-Ching Huang, Department of Bioscience and Biotechnology, National Taiwan Ocean University, 2, Pei-Ning Road, Keelung, 20224, Taiwan; Tel.: +886-2-24622192 ext5517; Fax: +886-2-24622320; E-mail: [email protected]
Table S1 List of microalgae strains used in this study.
Taxon
Strain
Order
Family
GenBank Accession no.
Emiliania huxleyi
CCMP373
Isochrysidales
Noelaerhabdaceae
KC404141
Isochrysis galbana
I001
Isochrysidales
Isochrysidaceae
KC888111
Thalassiosira weissflogii
Clone Vic
Thalassiosirales
Thalassiosiraceae
GQ281043
Skeletonema cf. costatum
Kao, Taiwan
Thalassiosirales
Skeletonemaceae
JN676163
Tetraselmis chui
Clone TA
Chlorodendrales
Chlorodendraceae
DQ207405
Nannochloris sp.
CKM
Chlorellales
Chlorellaceae
GQ122381
Table S2 Species-specific peak masses of I. galbana obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
100.0
55.5
13170.4
100.0
3.3
2315.3
100.0
3.8
7030.6
100.0
105.0
114.9
26091.0
109.0
15.7
10560.8
107.0
3.3
6090.1
105.0
109.0
3.2
703.0
110.0
5.1
3430.9
109.0
4.1
7755.4
107.0
110.0
3.1
677.0
110.3
5.9
3958.0
110.9
6.3
10257.5
109.0
110.3
3.5
768.0
112.0
4.4
2880.8
112.9
3.0
5710.1
110.0
110.9
4.9
1081.0
117.0
4.1
2632.1
114.1
6.5
10511.5
110.3
112.0
5.4
1174.0
119.0
10.5
6803.8
114.9
7.4
11967.5
110.9
114.1
4.9
1086.9
120.0
4.4
2807.5
115.9
4.2
6774.5
112.0
127.0
3.8
789.6
121.0
9.7
6232.5
119.0
3.9
6275.3
112.9
132.0
774.7
156800.3
123.0
3.0
1937.4
121.0
3.0
4916.3
114.1
135.0
296.0
59075.7
123.9
4.7
2971.5
122.9
7.3
11843.8
114.9
137.0
4.1
740.8
126.6
3.4
2128.2
123.9
19.4
37356.5
115.9
139.0
4.2
772.0
127.0
6.6
4145.4
124.9
17.8
34099.2
117.0
146.1
596.0
104265.9
130.0
7.1
4425.1
127.0
22.1
42338.3
119.0
162.1
74.8
11066.1
132.0
192.4
117415.3
132.0
64.9
125885.3
120.0
178.1
12.0
1480.7
135.0
45.0
27322.6
135.0
26.0
50161.8
121.0
179.1
12.4
1530.1
136.0
8.3
4987.2
143.9
5.1
8058.5
122.9
181.7
3.2
374.0
143.0
4.6
2340.5
145.0
7.3
11506.5
123.0
187.1
10.0
1119.1
145.0
7.2
3670.5
146.1
27.6
56579.3
123.9
188.3
7.3
795.6
149.1
7.3
3357.0
149.1
5.5
11308.6
123.9
192.2
12.2
1271.1
151.0
3.3
1739.3
151.0
7.6
15537.9
124.9
206.1
176.1
15837.3
152.0
5.4
2849.9
157.1
4.5
6705.3
126.6
218.1
4.9
378.5
159.1
5.6
2800.2
159.1
4.2
6296.8
127.0
220.1
4.9
377.3
177.1
4.3
1552.0
162.1
6.9
14315.0
130.0
226.0
98.4
7136.3
179.1
3.1
1325.5
165.1
5.1
7666.5
132.0
234.1
114.9
7642.7
187.1
3.5
1172.0
170.9
3.4
7435.3
135.0
250.1
24.2
1263.0
187.7
4.8
1990.4
187.1
4.9
11362.4
136.0
252.2
8180.5
459608.8
192.2
7.3
2462.0
206.1
8.7
20159.0
137.0
252.6
109.1
6130.3
195.3
7.9
8643.0
234.1
4.2
9084.4
139.0
423.0
11.1
452.3
199.2
3.8
1165.3
242.1
3.6
7758.8
143.0
562.4
315.5
10615.5
206.1
9.1
9852.6
246.4
5.8
12042.5
143.9
592.3
6.1
195.2
234.1
14.1
3369.0
252.2
142.5
295657.1
145.0
593.3
31.2
991.4
242.1
3.9
899.5
268.9
4.3
8368.4
146.1
614.3
9.7
291.8
430.4
13.5
2279.0
367.2
3.1
2505.8
149.1
638.3
25.0
721.8
460.2
11.9
1892.0
374.3
20.2
15286.8
151.0
639.2
33.0
950.8
462.3
6.2
990.0
376.0
25.2
18954.5
152.0
660.4
6.2
174.9
533.3
14.3
1964.0
408.0
22.2
15468.5
157.1
666.7
4.4
123.3
562.4
16.7
17348.2
532.2
6.2
3119.3
159.1
672.6
6.5
177.2
592.3
9.3
1085.5
533.3
7.1
3640.0
162.1
700.6
11.3
290.3
593.3
16.0
1855.0
562.4
109.6
51699.2
165.1
726.6
4.0
97.1
614.3
5.0
4986.4
614.3
8.3
3287.2
170.9
770.6
11.6
269.0
700.6
15.4
1240.0
691.0
4.2
1244.2
177.1
772.6
743.3
16919.7
726.6
7.4
505.0
772.6
97.3
13903.2
178.1
786.7
5.5
122.2
772.6
139.9
20509.8
786.7
6.0
808.2
179.1
798.6
16.5
368.0
786.7
8.2
462.0
800.6
137.0
17323.3
181.7
800.6
920.8
20000.1
800.6
174.1
24173.5
820.7
15.7
1732.5
187.1
820.7
4.5
94.9
820.7
12.9
687.0
822.7
5.0
549.6
187.7
822.7
4.5
95.7
826.7
37.0
4815.8
826.7
54.5
5876.3
188.3
826.7
193.5
4083.1
870.6
10.7
1280.3
828.7
18.3
1959.2
192.2
870.6
52.5
1045.8
871.5
11.9
1422.5
870.6
73.5
6104.3
195.3
871.5
83.8
1675.9
872.7
75.5
9022.2
871.5
60.4
5027.2
199.2
872.7
254.8
5070.4
880.7
3.5
414.7
872.7
169.1
14023.5
206.1
879.7
5.6
109.9
877.7
5.7
463.7
218.1
880.7
3.5
69.4
880.7
23.9
1928.5
220.1
881.7
20.8
1678.1
226.0
907.8
9.0
677.4
234.1
909.8
10.4
784.7
242.1
1011.8
5.9
274.3
246.4
250.1
252.2
252.6
268.9
367.2
374.3
376.0
408.0
423.0
430.4
460.2
462.3
532.2
533.3
562.4
592.3
593.3
614.3
638.3
639.2
660.4
666.7
672.6
691.0
700.6
726.6
770.6
772.6
786.7
798.6
800.6
820.7
822.7
826.7
828.7
870.6
871.5
872.7
877.7
879.7
880.7
881.7
907.8
909.8
1011.8
Table S3 Species-specific peak masses of E. huxleyi obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
100.0
140.4
45468.2
100.0
42.0
20938.0
100.0
20.5
6686.2
100.0
102.9
7.1
1974.8
107.0
10.2
4999.4
102.9
5.8
1871.4
102.9
105.0
23.1
6456.5
109.0
18.0
8833.8
107.0
8.9
2903.5
105.0
107.0
5.0
1406.5
110.3
5.6
2700.8
109.0
18.6
6088.3
107.0
109.0
5.1
1412.5
112.0
3.7
1768.3
110.3
5.7
1833.3
109.0
110.3
7.5
2058.5
112.3
5.7
2732.6
112.0
5.8
1857.1
110.3
110.9
8.0
2194.5
114.1
7.3
3478.9
114.1
4.2
1317.5
110.9
112.0
4.7
1286.5
117.0
8.7
4166.9
114.4
3.6
1120.5
112.0
114.1
23.1
7533.2
118.0
11.3
5394.7
116.0
4.1
1262.5
112.3
117.0
7.6
2468.7
119.0
9.9
4673.9
117.0
6.8
2097.5
114.1
118.0
19.7
6345.4
121.0
27.4
12908.5
118.0
18.5
5862.6
114.4
119.0
3.9
1263.7
123.0
5.2
2481.9
119.0
8.7
2747.9
116.0
120.0
3.2
872.3
132.0
86.5
40206.0
121.0
39.5
12539.1
117.0
121.0
33.2
10892.7
135.0
11.0
5050.3
123.0
4.3
1338.7
118.0
126.1
3.5
1123.9
143.0
3.9
1574.0
132.0
108.1
33748.4
119.0
132.0
136
45087.5
145.1
5.4
2406.5
135.0
8.9
2717.9
120.0
134.0
9.3
3006.7
159.1
4.5
1875.4
144.0
3.2
914.1
121.0
136.0
3.1
983.5
184.0
105.6
38373.0
145.1
3.4
1008.2
123.0
139.0
9.0
2887.9
549.2
18.7
3017.0
149.0
7.8
2292.3
126.1
140.0
7.7
2487.6
593.3
16.1
2338.5
158.1
10.4
2983.9
132.0
145.1
10.1
3212.4
608.2
8.5
1189.0
159.1
4.5
1280.0
134.0
147.0
5.4
1708.5
614.2
3.6
3187.2
184.0
122.6
32984.7
135.0
149.0
3.1
990.8
700.6
4.9
501.5
201.0
7.2
1499.5
136.0
154.1
6.5
2049.7
870.6
4.6
275.5
203.2
11
6120.8
139.0
161.1
3.2
989.8
871.6
5.6
340.4
228.3
78.3
43062.4
140.0
162.1
7.1
2183.3
872.6
12.9
775.9
252.1
24.2
13070.4
143.0
169.0
3.7
1118.9
284.3
4.2
2188.3
144.0
181.7
4.6
1332.4
303.6
3.2
1627.3
145.1
184.0
160.4
46202.3
402.4
4.9
632.0
147.0
226.1
6.4
946.5
494.6
14.4
4052.5
149.0
247.2
6.8
1478.8
522.6
30.4
8527.2
154.1
252.1
130.4
25934.4
549.2
24.1
10485.9
158.1
298.8
3.2
460.6
614.2
6.1
2615.9
159.1
398.2
3.7
291.1
660.4
7.3
691.0
161.1
434.9
6.0
443.9
672.6
9.8
883.0
162.1
456.9
4.5
314.7
870.6
12.9
629.3
169.0
593.3
4.0
209.0
871.6
10.6
515.0
181.7
614.2
13.3
656.8
872.6
15.0
731.6
184.0
867.6
3.7
106.8
1316.2
8.4
177.2
201.0
1318.1
12.1
257.0
203.2
1364.2
3.1
62.0
226.1
228.3
247.2
252.1
284.3
298.8
303.6
398.2
402.4
434.9
456.9
494.6
522.6
549.2
593.3
608.2
614.2
660.4
672.6
700.6
867.6
870.6
871.6
872.6
1316.2
1318.1
1364.2
Table S4 Species-specific peak masses of T. weissflogii obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
102.0
5.6
886.1
100.2
6.0
576.9
102.0
5.7
2222.7
100.2
110.0
11.8
1759.9
101.0
3.4
329.0
113.6
13.9
5055.9
101.0
113.6
26.6
3839.4
102.0
5.0
479.6
117.0
4.2
1501.6
102.0
118.0
4.3
614.6
109.1
32.2
2971.8
130.0
13.1
4481.5
109.1
120.0
7.5
1049.2
110.0
21.8
1988.0
132.1
389.6
131292.4
110.0
132.1
940.9
118848.3
110.3
18.8
1711.5
136.0
9.9
3309.3
110.3
134.9
4.4
546.0
112.3
32.7
2957.5
152.1
5.6
1715.7
112.3
136.0
5.4
662.4
113.6
39.7
3581.5
178.1
9.5
2425.3
113.4
140.0
12.1
1282.0
113.4
21.2
1917.5
184.1
170.2
41750.0
113.6
140.3
18.4
1959.0
117.0
3.6
317.6
218.1
9.3
2775.5
117.0
142.1
5.1
545.0
118.0
8.1
723.8
252.1
97.0
26554.4
118.0
178.1
22.0
1716.5
119.0
17.2
1577.1
365.1
32.5
3538.8
119.0
184.1
568.2
39224.3
120.0
22.5
2038.1
394.4
3.1
342.4
120.0
198.1
3.0
175.2
121.1
10.9
949.0
396.4
3.9
441.4
121.1
218.1
34.9
1796.7
125.1
9.6
819.8
444.3
5.2
632.0
125.1
226.1
8.7
419.5
125.9
3.7
316.7
459.2
29.8
3658.6
125.9
252.1
284.5
12811.1
127.1
10.0
858.2
460.2
13.7
1674.6
127.1
276.1
4.4
200.0
127.9
16.2
1445.5
461.3
8.5
1041.6
127.9
308.3
3.4
155.7
130.0
22.8
1923.8
467.2
4.1
505.0
130.0
365.1
7.0
343.4
132.1
1285.6
107316.6
472.3
4.4
546.6
132.1
459.2
11.9
597.0
134.9
4.8
392.4
481.2
10.5
1562.0
134.9
472.3
3.3
163.3
136.0
15.5
1273.9
490.3
23.5
2941.4
136.0
484.0
4.9
226.2
138.7
4.7
373.2
506.0
23.7
2760.0
138.7
490.3
11.9
596.9
139.1
6.6
531.4
518.3
6.4
806.0
139.1
506.0
10.7
495.1
145.1
6.9
533.9
519.2
19.3
2430.0
140.0
519.2
8.1
407.4
147.1
13.7
1037.0
527.1
36.3
4581.4
140.3
527.1
9.9
500.5
149.1
7.0
513.2
533.2
22.7
3296.1
142.1
533.2
8.3
421.3
178.1
16.8
1031.7
536.4
6.7
972.5
145.1
592.2
6.7
336.1
184.1
519.1
28957.3
549.3
3.0
444.6
147.1
593.2
8.8
440.8
218.1
14.6
688.1
562.3
3.5
515.6
149.1
614.1
14.8
740.5
225.2
3.8
169.6
591.4
5.8
854.5
152.1
638.1
8.7
468.5
252.1
154.3
6513.2
592.2
14.5
2138.0
178.1
639.0
10.4
556.5
282.3
3.4
142.0
593.2
17.6
2589.4
184.1
672.4
11.3
617.0
308.3
6.9
299.9
614.1
28.8
4213.7
198.1
698.4
36.8
2039.5
339.3
3.7
161.5
672.4
12.2
1858.0
218.1
700.4
43.9
2444.9
365.1
20.3
921.9
689.2
7.9
1233.3
225.2
712.4
3.0
166.4
445.2
13.1
600.5
698.4
23.4
3760.3
226.1
722.4
13.3
747.4
459.2
22.2
1017.5
700.4
27.9
4500.1
252.1
724.4
78.6
4421.7
460.2
11.5
527.4
722.4
10.2
1683.8
276.1
726.4
140.4
7842.8
467.2
4.9
224.6
724.4
42.4
7009.7
282.3
728.5
7.8
435.6
481.2
12.1
545.9
726.4
67.5
11033.9
308.3
738.4
6.1
341.7
484.0
7.1
303.0
736.5
3.3
528.9
339.3
746.4
87.4
4910.8
490.3
17.7
804.4
738.4
6.3
1001.9
365.1
748.4
34.7
1940.3
506.0
32.5
1418.2
746.4
48.9
7748.9
394.4
750.4
53.5
2979.6
507.6
14.9
670.9
748.4
22.0
3455.9
396.4
752.4
142.6
7935.3
509.5
12.9
581.5
750.4
33.4
5210.6
444.3
754.5
123.9
6835.1
511.5
18.6
842.3
752.4
67.2
10418.0
445.2
760.4
6.3
341.2
513.5
14.7
659.9
754.5
60.8
9380.7
459.2
762.4
3.4
182.5
515.6
7.3
327.1
772.4
135.6
16530.5
460.2
770.4
13.8
620.0
518.3
3.4
152.5
774.4
157.9
19073.0
461.3
772.4
236.2
12497.2
519.2
14.7
662.8
776.5
41.2
4958.1
467.2
774.4
283.5
14936.3
527.1
26.4
1193.2
778.6
15.9
1913.0
472.3
776.5
66.8
3502.6
533.2
22.9
1032.2
780.5
7.1
851.4
481.2
780.5
11.8
613.9
562.3
4.3
193.5
786.5
7.9
919.7
484.0
786.5
10.4
533.6
571.2
6.3
282.7
788.5
4.3
500.7
490.3
788.5
4.6
237.2
592.2
16.3
726.1
794.5
4.3
488.8
506.0
794.5
4.7
239.7
593.2
12.9
577.8
796.5
13.2
1491.4
507.6
796.5
17.8
900.8
614.1
63.4
2798.0
798.4
96.5
10861.2
509.5
798.4
148.6
7485.4
660.3
3.3
141.5
800.4
216.8
24261.1
511.5
800.4
388.8
19559.1
672.4
12.3
540.2
814.5
3.7
394.0
513.5
814.5
4.1
201.0
698.4
32.0
1403.2
820.4
41.7
4406.6
515.6
820.4
56.0
2697.4
700.4
44.2
1952.5
822.5
26.2
2756.2
518.3
822.5
33.5
1606.2
722.4
9.6
420.6
824.5
18.9
1977.6
519.2
824.5
24.7
1177.2
724.4
74.8
3266.7
826.5
10.0
1035.2
527.1
826.5
12.6
598.9
726.4
135.4
5860.1
846.4
122.5
12302.5
533.2
846.4
177.4
8083.3
746.4
98.3
4095.7
851.2
3.7
352.2
536.4
870.4
22.3
956.3
748.4
35.9
1486.4
870.4
37.6
3500.0
549.3
871.4
22.6
970.9
750.4
52.0
2150.9
871.4
35.3
3281.9
562.3
752.4
147.4
6109.4
966.9
11.2
428.0
571.2
754.5
126.8
5215.9
968.9
18.3
703.0
591.4
772.4
241.0
9504.1
981.0
6.2
219.0
592.2
774.4
325.3
12780.6
983.0
8.4
296.0
593.2
776.5
69.8
2724.7
614.1
780.5
9.6
373.2
638.1
786.5
7.4
280.3
639.0
788.5
3.5
132.1
660.3
794.5
4.2
157.4
672.4
796.5
13.4
495.4
689.2
798.4
154.2
5711.1
698.4
800.4
451.5
16607.7
700.4
820.4
74.7
2626.5
712.4
822.5
42.9
1505.7
722.4
824.5
26.5
925.3
724.4
826.5
10.4
362.4
726.4
836.5
3.4
116.9
728.5
846.4
229.4
7774.4
736.5
870.4
57.4
1825.1
738.4
871.4
34.6
1097.1
746.4
966.9
4.8
111.5
748.4
968.9
10.8
248.1
750.4
752.4
754.5
760.4
762.4
770.4
772.4
774.4
776.5
778.6
780.5
786.5
788.5
794.5
796.5
798.4
800.4
814.5
820.4
822.5
824.5
826.5
836.5
846.4
851.2
870.4
871.4
966.9
968.9
981.0
983.0
Table S5 Species-specific peak masses of Nannochloris sp. obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
118.0
5.9
228.8
102.0
15.9
5873.6
102.0
34.8
4403.3
102.0
128.0
5.9
219.0
138.0
3.7
1257.5
118.0
5.5
667.2
118.0
138.0
11.2
407.8
168.1
4.4
1405.3
138.0
10.1
1202.6
128.0
140.0
4
146.5
170.1
8.0
2552.3
170.1
3.0
371.0
138.0
170.1
4.9
146.5
184.0
27.1
8381.4
184.0
28.5
3610.3
140.0
175.1
25.2
757.9
230.1
5.6
1258.0
269.0
14.1
1114.0
168.1
184.0
171
4948.4
269.0
6.1
1244.0
319.9
15.5
1165.0
170.1
203.9
3.9
97.7
365.2
14.0
2495.0
614.2
28.1
1828.3
175.1
614.2
6.5
100.9
614.2
3.2
3917.2
660.4
13.0
785.0
184.0
660.4
6.6
96.5
660.4
4.9
563.0
203.9
230.1
269.0
319.9
365.2
614.2
660.4
Table S6 Species-specific peak masses of S. cf. costatum obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
101.0
3.7
122.6
100.0
10.6
240.5
101.0
8.3
1662.4
100.0
102.0
4.7
154.7
101.0
7.3
167.3
102.0
12.5
2495.7
101.0
110.0
64.5
2079.6
102.0
13.7
313.9
120.0
58.4
10944.0
102.0
112.0
79.3
2555.5
107.0
3.2
71.4
129.1
27.9
4986.2
107.0
113.1
25.6
815.0
110.0
61.0
1354.4
130.0
35.8
6344.7
110.0
120.0
56.8
1893.5
120.0
103.6
2270.0
132.1
26.4
4644.6
112.0
125.1
4.5
138.7
125.1
20.8
454.0
134.0
3.9
687.7
113.1
127.1
5.0
154.2
127.9
70.6
1567.9
136.0
71.0
12385.6
120.0
129.1
74.5
2275.5
129.9
81.2
1791.1
152.0
54.6
8453.3
125.1
132.1
18.4
569.1
130.0
79.9
1708.9
184.1
279.4
33109.1
127.1
133.0
7.6
230.5
132.1
23.8
514.1
252.2
43.7
3301.6
127.9
136.0
31.0
947.3
136.0
54.7
1142.6
252.8
74.5
5574.8
129.1
141.1
4.1
117.6
146.1
6.2
131.0
591.4
3.1
212.3
129.9
146.1
7.2
208.7
147.0
3.7
75.0
592.3
4.0
276.2
130.0
147.0
4.1
117.7
152.0
13.4
268.2
593.4
6.1
426.1
132.1
152.0
7.2
200.7
154.1
24.1
489.6
133.0
154.1
46.8
1285.6
159.1
4.6
92.5
134.0
184.1
648.5
15922.0
162.1
4.6
90.1
136.0
215.0
81.8
1825.8
184.1
603.0
11166.3
141.1
252.2
16.9
362.7
186.1
58.9
1096.0
146.1
252.2
14.5
244.8
147.0
308.3
25.2
422.8
152.0
154.1
159.1
162.1
184.1
186.1
215.0
252.2
252.8
308.3
591.4
592.3
593.4
Table S7 Species-specific peak masses of T. chui obtained using HgSe, HgTe, HgTeSe, or HgSeHgTeHgTeSe NMs as substrates.
HgSe
HgTe
HgTeSe
HgSeHgTeHgTeSe
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
S/N
Intens.
m/z
100.0
13.1
2069.8
100.0
14.3
2003.9
110.2
17.8
12900.7
100.0
101.0
3.2
513.8
101.0
7.0
975.5
125.9
4.8
3271.7
101.0
102.0
4.8
754.5
102.0
14.0
1974.0
127.0
5.4
3660.3
102.0
110.2
39.2
6036.5
107.0
6.3
864.2
129.1
7.2
4903.9
107.0
112.0
24.1
3706.0
110.2
101.2
13409.0
130.0
17.7
12017.4
110.2
120.0
15.5
2327.5
112.0
43.5
5825.7
131.1
4.6
3060.2
112.0
125.0
20.0
2864.6
114.0
7.7
1016.8
133.1
4.7
3150.6
114.0
127.0
3.0
431.7
116.0
10.6
1447.4
134.9
11.3
7509.7
116.0
130.0
8.5
1208.8
118.0
4.0
517.0
136.0
38.0
25321.5
118.0
134.9
20.0
2679.8
119.0
4.8
620.2
138.1
9.8
6442.9
119.0
136.0
31.8
4231.7
120.0
31.6
4151.6
152.0
23.1
13881.4
120.0
138.1
3.3
441.6
121.1
6.5
843.1
159.1
10.3
5800.9
121.1
147.0
11.8
1487.5
125.0
5.3
666.7
160.1
3.8
2120.2
125.0
152.0
9.9
1176.9
125.9
7.0
884.9
166.1
8.0
4233.3
125.9
157.2
4.9
555.2
127.0
6.9
873.1
184.1
481.6
209083.3
127.0
166.1
15.1
1629.7
127.9
16.7
2165.7
198.1
10.5
4495.4
127.9
175.1
107.3
10754.8
129.1
12.9
1608.4
199.2
37.5
16074.6
129.1
184.1
1103.1
102470.5
129.9
19.8
2585.7
202.2
3.0
1239.9
129.9
199.2
31.5
2745.3
130.0
36.1
4532.0
206.1
4.7
1919.1
130.0
236.2
7.7
551.2
134.9
15.7
1877.9
207.1
3.1
1275.3
131.1
308.3
4.1
255.9
136.0
71.6
8673.9
236.2
7.3
2412.1
133.1
467.1
6.2
425.9
138.1
6.1
717.9
268.1
5.6
1350.0
134.9
481.1
16.5
1143.0
148.0
23.7
2646.3
281.1
6.6
1735.6
136.0
505.5
30.1
2023.5
152.0
24.9
2588.4
459.3
5.9
1713.8
138.1
614.1
4.4
270.5
157.2
4.5
445.5
460.3
3.8
1080.6
147.0
704.5
6.8
357.0
159.1
14.9
1441.0
467.1
9.4
2691.8
148.0
734.5
3.4
171.9
160.1
5.6
551.7
468.2
3.8
1079.7
152.0
758.5
6.4
311.0
166.1
11.6
1064.5
481.1
18.5
5130.7
157.2
175.1
264.9
22755.2
483.4
4.9
1279.5
159.1
184.1
1474.4
117038.0
495.1
4.5
1226.7
160.1
198.1
9.1
683.8
497.1
4.2
1147.6
166.1
206.1
5.7
406.3
505.5
28.6
7448.6
175.1
236.2
10.6
618.0
533.2
3.1
965.5
184.1
467.1
24.2
1769.0
571.2
3.4
1025.1
198.1
481.1
52.5
3833.8
614.1
8.4
2349.9
199.2
495.1
6.7
488.3
704.5
32.0
6445.8
202.2
497.1
6.1
443.6
706.6
4.6
919.9
206.1
505.5
80.6
5621.9
732.6
5.1
959.0
207.1
519.7
8.6
571.6
734.5
19.5
3686.0
236.2
533.2
3.0
231.3
736.6
13.0
2440.8
268.1
555.2
4.4
325.9
758.5
29.6
5303.3
281.1
571.2
7.4
532.9
760.5
13.2
2354.3
308.3
593.2
3.1
243.3
780.6
8.8
666.8
459.3
614.1
19.0
1500.3
782.5
17.9
1350.8
460.3
704.5
14.8
1134.8
784.5
34.2
2577.0
467.1
734.5
5.9
440.6
786.6
6.1
455.8
468.2
736.6
4.8
354.0
804.5
39.2
2758.8
481.1
758.5
9.9
712.5
806.5
43.7
3076.4
483.4
760.5
5.2
375.8
808.6
6.8
473.2
495.1
782.5
4.2
272.6
870.5
8.7
489.9
497.1
784.5
8.8
577.1
871.6
9.5
534.8
505.5
804.5
9.9
637.3
519.7
806.5
11.9
771.7
533.2
555.2
571.2
593.2
614.1
704.5
706.6
732.6
734.5
736.6
758.5
760.5
780.6
782.5
784.5
786.6
804.5
806.5
808.6
870.5
871.6
Table S8 Reproducibility of the LDI-MS analysis of the six microalgae by the three HgSe, HgTe, and HgTeSe nanomatrices from five repetitive measurements.
Algae
Nanomatrices
No. of significant peaks
(fed to Venn diagram construction)
Average RSD (%)
I. galbana
HgSe
54
9.1
HgTe
52
11
HgTeSe
58
9.2
E. huxleyi
HgSe
39
20
HgTe
26
10.2
HgTeSe
42
5.1
T. weissflogii
HgSe
68
8.8
HgTe
90
4.0
HgTeSe
72
13.7
Nannochloris sp.
HgSe
10
9.8
HgTe
10
12.9
HgTeSe
9
10.9
S. cf. costatum
HgSe
20
17.3
HgTe
22
14.3
HgTeSe
15
17.0
T. chui
HgSe
28
5.8
HgTe
54
9.1
HgTeSe
51
9.7
Fig. S1 EDS spectra of prepared (a) HgSe, (b) HgTe, and (c) HgTeSe NMs.
Fig. S2 STEM-EDS elemental mapping of the prepared (a) HgSe, (b) HgTe, and (c) HgTeSe NMs.
Fig. S3 UV-vis absorption spectra of the prepared HgTe, HgSe, and HgTeSe NMs (0.025X) in DI water. Absorbance (Abs) is plotted in arbitrary units (a. u.).
Fig. S4 XRD spectra of (a) HgSe, (b) HgTe, and (c) HgTeSe NMs.
Fig. S5 Light microscope (400X) images of the tested microalgae; (a) I. galbana, (b) E. huxleyi, (c) T. weissflogii, (d) Nannochloris sp, (e) S. cf. costatum, and (f) T. chui.
Fig. S6 Background mass spectra recorded using (a) HgSe NMs, (b) HgTe NMs, (c) HgTeSe NMs, (d) DHB, (e) HCCA, and (f) SA as matrices. Other conditions were the same as those described in Fig. 1
S2