Stat1 IGF-IR IFN- - Bio-Rad · IGF-IR IL-17A/F IL-12p70 Stat1 ... Bio-Plex Pro™ Human Inflammation Panel 1 For the first time, ... Bio-Plex Comprehensive Analyte Tables 41 1

PDGFR-a

c-Jun

EGFR

GSK-3a/b

Erk1/2

NF-kb p65

JNK

IFN-b

IL-19

c-Jun

IGF-IR

IL-17A/F

IL-12p70Stat1

TGF-b1

c-Jun

TGF-b1

Akt

p70 S6 K

Analyte Guide 2016

Multiplex Suspension Arrays

Bio-Plex® Multiplex Immunoassays

© 2016 Bio-Rad Laboratories, Inc.

Discover More with the Latest in Bio-Plex Assays

Bio-Plex Pro™ Human Inflammation Panel 1 For the first time, the most popular inflammation biomarkers, including the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs, can be measured using a single multiplex kit (page 8). Available in larger 24-plex and 37-plex screening panels, a pathway-focused Treg 12-plex panel, and singleplex and custom-configured assays, the Bio-Plex Pro Human Inflammation Assays include the following analytes:

Bio-Plex Pro RBM Human Metabolic and Hormone Assays Developed in close collaboration with leading metabolic researchers, this highly relevant panel consists of 39 assays, including 15 that are offered only by Bio-Rad (page 23).

Bio-Plex Pro Human MMP and TIMP AssaysThe only Luminex panel on the market enabling users to investigate 9 MMPs in one sample at a single dilution (page 12).

Coming Soon

Bio-Plex Pro Human Cytokines, 48-PlexThe largest Bio-Plex Human Screening Panel, with unparalleled performance.

Coming Soon

Bio-Plex Pro Mouse Cytokines, 33-PlexNew mouse cytokine assays for larger multiplex screening studies and custom-configured smaller multiplex kits for pathway-specific research. Includes the fastest protocols with best-in-class performance.

Coming Soon

Bio-Plex Pro Human Neuroscience and Apolipoprotein PanelsNew Human Neuroscience Panels focused on targets relevant to neurodevelopment and neurodegenerative diseases. Developed for use with serum, plasma, and CSF samples, these panels combine the best sensitivity and reproducibility available in multiplex neuroscience assays.

APRIL / TNFSF13BAFF / TNFSF13BsCD30 / TNFRSF8sCD163Chitinase 3-like 1gp130 / sIL-6RbIFN-a2IFN-bIFN-gIL-2

sIL-6RaIL-8 / CXCL8IL-10IL-11IL-12 (p40)IL-12 (p70)IL-19IL-20IL-22IL-26

L-27 (p28)IL-28A / IFN-l2IL-29 / IFN-l1IL-32IL-34IL-35LIGHT / TNFSF14MMP-1MMP-2MMP-3

OsteocalcinOsteopontinPentraxin-3sTNF-R1sTNF-R2TSLPTWEAK / TNFSF12

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Table of Contents

The Power of Bio-Plex Assays 2Bio-Plex Assay Configurations 4Human Assays 6 Cytokine Group l 6 Cytokine Group ll 7 Inflammation New 8 MMP and TIMP 12 Th17 Cytokine 13 Chemokines 14 TGF-b 16 Cancer Biomarkers 17 Cell Signaling Assays 19 Apoptosis 23 Diabetes 24 Metabolic and Hormone 25 Kidney Toxicity 27 Isotyping 28 Acute Phase 29

Mouse Assays 30 Cytokine Group l 30 Cytokine Group ll 31 Cytokine Group lll 31 TGF-b 31 Cell Signaling Assays 32 Diabetes 34Rat Assays 35 Cytokine 35 TGF-b 35 Cell Signaling Assays 36 Diabetes 38 Kidney Toxicity 39Canine Assays 40 Kidney Toxicity 40 Bio-Plex Comprehensive Analyte Tables 41

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2

The Power of Bio-Plex Assays Bio-Plex Multiplex Assays deliver more data in less time with less sample and with fewer manual manipulations. The examples on the next page show a comparison between a Bio-Plex assay and conventional methods. In the top panel, a Bio-Plex Phosphoprotein 5-Plex Assay is compared with five western blots. In the bottom panel, a Bio-Plex Human Cytokine 27-Plex Assay is compared with 27 ELISA assays.

Proven Results The Bio-Plex System is the most widely cited multiplex assay platform in life science research today. Application areas include inflammation, autoimmune disease, asthma, diabetes/obesity, cancer, neurological disorders, cardiovascular disease, drug discovery, and vaccine development.

Open PlatformBio-Plex Pro Magnetic Assays, configured on MagPlex Beads, are compatible with all xMAP life science instruments supplied by any Luminex partner. Choose from a broad selection of preconfigured and custom assay panels.

Bio-Plex Manager™ Software supports all Luminex 100 and 200 Instruments. In addition, it supports both magnetic and nonmagnetic beads and can analyze data from any xMAP platform. Kits are available to run Bio-Plex Manager on Luminex Assay Readers. For more information, contact your local Bio-Rad sales representative.

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PDGFR-a

c-Jun

EGFR

GSK-3a/b

Erk1/2

NF-kb p65

JNK

IFN-b

IL-19

c-Jun

IGF-IR

IL-17A/F

IL-12p70Stat1

TGF-b1

c-Jun

TGF-b1

Akt

p70 S6 K3

Analysis of five phosphoproteins in 12 samples.

Western Blot Bio-Plex System

Number of phosphoproteins 5 5

Number of samples 12 12

Total data points 60 60

Number of gels or 96-well plates 5 1

Data points per gel or plate 12 60

Total time required ~8 hr + one 2 hr + one overnight incubation overnight incubation

Sample volume 125 µl 50 µl

Dynamic range Limited total protein 0.04–10 µg/well*

* The range of total lysate protein in which the analyte can be detected.

Analysis of 27 cytokines in 80 samples.

ELISA Bio-Plex System

Number of cytokines 27 27

Number of samples 80 80

Total data points 2,160 2,160

Number of 96-well plates 27 1

Data points per plate 80 2,160

Total time required >60 hr 3 hr

Sample volume Serum or plasma, Serum or plasma, >1 ml* 12.5 µl Cell culture supernatant, Cell culture supernatant, >1 ml* 50 µl

* Based on 50 µl/well of sample.


4

Bio-Plex Assay Configurations — A Multitude of ChoicesBio-Rad offers a continually growing line of assays and associated products for extracellular and intracellular analysis. The assays are relevant to researchers studying everything from cancer to cardiovascular disease and available for use with multiple species and sample types. For the most up-to-date target information, go to bio-rad.com/bioplex.

Find Your Bio-Plex AssayUse our web tool to quickly and easily locate currently available assays. Search by species, analyte name, or disease area.

n Browse the entire catalog of analytes alphabetically

n Filter by species and research area

n Find all relevant assays based on search criteria — results grouped for easier product selection

Go to bio-rad.com/FindMyAssay

Bio-Plex Assay BuilderDesign your own custom assays from our selection of hundreds of analytes in four easy steps. With the Assay Builder you can:

n Get a price quote

n Place your order online

n Look up an existing assay by catalog number

Go to bio-rad.com/assaybuilder

x-Plex™ Assay

We Mix

Express Assay

You Mix

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Visit bio-rad.com/DataPro and try Bio-Plex Data Pro free for 30 days.

Over 50% of multiplex assays worldwide are run using Bio-Plex Software.

Try Bio-Plex Manager and Bio-Plex Data Pro™ Software and find out why.


6

Bio-Plex Pro Human Cytokine Group I AssaysHuman Assays

Bio-Plex Pro Human Cytokine 17-Plex Panel (M5000031YV)

G-CSF IL-8 / CXCL8

GM-CSF IL-10

IFN-g IL-12 (p70)

IL-1b IL-13

IL-2 IL-17A

IL-4 MCP-1 / CCL2

IL-5 MIP-1b IL-6 TNF-a IL-7

Bio-Plex Pro Human Cytokine 27-Plex Panel (M50-0KCAF0Y)

Eotaxin / CCL11 IL-10

FGF-basic IL-12970

G-CSF IL-13

GM-CSF IL-15

IFN-g IL-17A

IL-1b IL-12 (p70)

IL-1Ra MCP-1 / CCL2

IL-2 MIP-1a IL-4 MIP-1b IL-5 PDGF-BB

IL-6 RANTES

IL-7 TNF-a IL-8 / CXCL8 VEGF

IL-9

Bio-Plex Pro Human Cytokine Th1/Th2 Panel (M5000005L3)

GM-CSF IL-10

IFN-g IL-12 (p70)

IL-2 IL-13

IL-4 TNF-a IL-5

Bio-Plex Pro Human Cytokine 8-Plex Panel (M50000007A)

GM-CSF IL-6

IFN-g IL-8 / CXCL8

IL-2 IL-10

IL-4 TNF-a

bio-rad.com/CCGFassaysBio-Rad bulletin 5828

Performance Characteristics Assay Working Range, Assay Sensitivity, pg/ml pg/ml Assay Precision

Target LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV

Group I 27-Plex Panel

Eotaxin / CCL11 40.9 5,824 2.5 8 11

FGF-basic 27.2 7,581 1 .9 8 8

G-CSF 2.4 11,565 1.7 10 5

GM-CSF 63.3 6,039 2 .2 12 6

IFN-g 92.6 52,719 6.4 15 9

IL-1b 3.2 3,261 0.6 6 8

IL-1Ra 81.1 70,487 5.5 9 8

IL-2 2.1 17,772 1.6 7 9

IL-4 2.2 3,467 0.7 9 8

IL-5 3.1 7,380 0.6 8 10

IL-6 2.3 18,880 2.6 7 11

IL-7 3.1 6,001 1.1 6 8

IL-8 / CXCL8 1.9 26,403 1.0 9 4

IL-9 2.1 7,989 2.5 8 9

IL-10 2.2 8,840 0.3 5 6

IL-12 (p70) 3.3 13,099 3.5 6 6

IL-13 3.7 3,137 0.7 8 7

IL-15 2.1 2,799 2.4 5 6

IL-17A 4.9 12,235 3.3 8 6

IP-10 18.8 26,867 6.1 11 9

MCP-1 / CCL2 2.1 1,820 1.1 9 7

MIP-1a 1.4 836 1.6 7 8

MIP-1b 2.0 1,726 2.4 8 8

PDGF-BB 7.0 51,933 2.9 9 8

RANTES 2.2 8,617 1.8 9 6

TNF-a 5.8 95,484 6.0 8 6

VEGF 5.5 56,237 3.1 9 7

Bio-Plex Pro Human Cytokine, Chemokine, and Growth Factor Magnetic Bead–Based Assays detect and measure levels of analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth to infections to Parkinson’s disease.

Bio-Plex Pro Human Group l Analytes

Analyte Singleplex Bead Region

Eotaxin 171B5015M 43

FGF-basic 171B5016M 44

G-CSF 171B5017M 57

GM-CSF 171B5018M 34

IFN-g 171B5019M 21

IL-b 171B5001M 39

IL-1Ra 171B5002M 25

IL-2 171B5003M 38

IL-4 171B5004M 52

IL-5 171B5005M 33

IL-6 171B5006M 19

IL-7 171B5007M 74

IL-8 / CXCL8 171B5008M 54

IL-9 171B5009M 77

IL-10 171B5010M 56

IL-12 (p70) 171B5011M 75

IL-13 171B5012M 51

IL-15 171B5013M 73

IL-17A 171B5014M 76

IP-10 171B5020M 48

MCP-1 / CCL2 171B5021M 53

MIP-1a 171B5022M 55

MIP-1b 171B5023M 18

PDGF-BB 171B5024M 47

RANTES 171B5025M 37

TNF-a 171B5026M 36

VEGF 171B5027M 45

http://www.bio-rad.com/evportal/destination/product?catID=b30acf0c-b9fc-458c-9f88-9414e9dfa9f7

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Bio-Plex Pro Human Cytokine Group ll Analytes


CTACK 171B6006M 72

Gro-a / KC / CXCL1 171B6007M 61

HGF 171B6008M 62

ICAM-1** 171B6009M 12

IFN-a2 171B6010M 20

IL-1a 171B6001M 63

IL-2Ra 171B6002M 13

IL-3 171B6003M 64

IL-12 (p40) 171B6004M 28

IL-16 171B6005M 27

IL-18* — 42

LIF 171B6011M 29

MCP-3 / CCL7 171B6012M 26

M-CSF 171B6013M 67

MIF 171B6014M 35

MIG / CXCL9 171B6015M 14

b-NGF 171B6016M 46

SCF 171B6017M 65

SCGF-b 171B6018M 78

SDF-1a+b / CXCL12 171B6019M 22

TNF-b 171B6020M 30

TRAIL 171B6021M 66

VCAM-1** 171B6022M 15

* Not available as a singleplex.

** ICAM-1 and VCAM-1 can be multiplexed together but not with other analytes.

Bio-Plex Pro Human Cytokine Group ll 21-Plex Panel (MF0005KMII)

CTACK / CCL27 MCP-3 / CCL7

Gro-a / KC / CXCL1 M-CSF

HGF MIF

IFN-a2 MIG / CXCL9

IL-1a b-NGF

IL-2Ra SCF

IL-3 SCGF-b IL-12 (p40) SDF-1a+b / CXC12

IL-16 TNF-b IL-18 TRAIL

LIF

Bio-Plex Pro Human Cytokine Group II AssaysPerformance Characteristics Assay Working Range, Assay Sensitivity, pg/ml pg/ml Assay Precision


Group II 21-Plex Panel, ICAM-1 and VCAM-1

CTACK / CCL27 13 16,874 3.4 5 6

Gro-a / KC / CXCL1 19 2,711 6.3 5 8

HGF 9.5 25,357 4.9 5 6

ICAM-1 13 26,368 2.4 4.3 3.8

IFN-a2 307 3,438 4.3 7 3

IL-1a 1.4 22,569 0.5 4 4

IL-2Ra 299 7,338 2.1 6 4

IL-3 12 22,803 4.8 7 4

IL-12 (p40) 41 28,662 23.3 5 8

IL-16 190 12,133 0.4 6 4

IL-18 1.8 28,677 0.2 4 5

LIF 12 22,184 5.5 4 3

MCP-3 / CCL7 79 11,234 1.0 7 8

M-CSF 2.0 32,200 0.9 4 5

MIF 231 24,373 1.5 5 8

MIG / CXCL9 2.7 8,584 1.2 6 6

b-NGF 1.0 4,247 0.2 4 7

SCF 1.5 25,078 1.0 5 4

SCGF-b 66 47,862 45.4 6 8

SDF-1a+b / CXCL12 10 10,391 8.7 6 6

TNF-b 1.5 24,505 0.3 4 4

TRAIL 4.4 44,769 2.1 4 8

VCAM-1 38 21,430 0.6 6.7 5.5




8

Bio-Plex Pro Human Inflammation Assaysbio-rad.com/inflammationassays

Bio-Rad bulletin 6625

For the first time, key inflammation biomarkers, including the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs, can be measured using a single multiplex kit. Available in larger 24-plex and 37-plex screening panels, a pathway-focused Treg 12-plex panel, and singleplex and custom-configured assays.

Bio-Plex Pro Human Inflammation AnalytesBio-Plex Pro Human Inflammation Panel

Analyte37-Plex

171AL001M24-Plex

171AL002M

Bio-Plex Pro Human Treg Cytokine Panel, 12-Plex

171AL003M Singleplex Sets Bead Region

APRIL / TNFSF13† • • 171BL001M 42

BAFF / TNFSF13B† • • 171BL002M 37

sCD30 / TNFRSF8 • • 171BL003M 53

sCD163† † • • 171BL004M 46

Chitinase 3-like 1† † • • 171BL005M 72

gp130 / sIL-6Rb • • 171BL006M 14

IFN-a2† † † • 171BL007M 20

IFN-b† • • 171BL008M 44

IFN-g • 171BL009M 21

IL-2 • • 171BL010M 38

sIL-6Ra • 171BL011M 19

IL-8 / CXCL8 • 171BL012M 54

IL-10 • • 171BL013M 56

IL-11† † † • • 171BL014M 39

IL-12 (p40)† † † • • 171BL015M 28

IL-12 (p70) • • 171BL016M 75

IL-19† † • • • 171BL017M 29

IL-20† † † • • • 171BL018M 30

IL-22 • • 171BL019M 18

IL-26† • • • 171BL020M 22

IL-27 (p28) • • • 171BL021M 13

IL-28A / IFN-l2 • • • 171BL022M 66

IL-29 / IFN-l1† † † • • • 171BL023M 33

IL-32† • • 171BL024M 35

IL-34† † • • 171BL025M 15

IL-35† • • • 171BL026M 34

continues

New


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† Of the top Luminex partners, Bio-Rad is the exclusive provider of this assay.† † Assay not available from Vendor M.† † † Assay not available from Vendor R.

Bio-Plex Pro Human Inflammation AnalytesBio-Plex Pro Human Inflammation Panel

Analyte37-Plex

171AL001M24-Plex

171AL002M

Bio-Plex Pro Human Treg Cytokine Panel, 12-Plex

171AL003M Singleplex Sets Bead Region

LIGHT / TNFSF14† † † • • 171BL027M 51

MMP-1 • 171BL028M 43

MMP-2 • 171BL029M 26

MMP-3 • 171BL030M 45

Osteocalcin† † † • • 171BL031M 65

Osteopontin • 171BL032M 77

Pentraxin-3 • • 171BL033M 48

sTNF-R1 • • 171BL034M 73

sTNF-R2 • • 171BL035M 67

TSLP† † † • • 171BL036M 52

TWEAK / TNFSF12† † † • • 171BL037M 62



10



Performance CharacteristicsAssay Working Range,

pg/ml*Assay Sensitivity,

pg/ml* Assay Precision*

Analyte LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV

APRIL/TNFSF13† 3,440.1 2,507,800.0 190.0 4.2 17.3

BAFF/TNFSF13B† 91.5 200,008.9 34.7 1.6 5.2

sCD30/TNFRSF8 53.4 12,973.1 1.0 2.5 5.0

sCD163† † 1,338.7 975,916.6 16.8 4.2 8.1

Chitinase 3-like 1† † 36.7 80,275.3 10.3 2.5 6.0

gp130/sIL-6Rb 257.6 18,785.6 16.9 2.3 5.9

IFN-a2† † † 3.1 6,702.2 0.7 3.6 10.0

IFN-b† 0.9 1,872.8 2.0 3.0 11.2

IFN-g 6.3 13,694.9 0.0 3.7 7.3

IL-2 1.2 2,662.7 0.1 3.3 10.3

sIL-6Ra 18.6 40,675.1 1.5 3.1 3.2

IL-8 / CXCL8 4.5 9,762.7 2.7 4.5 7.7

IL-10 1.7 3,781.2 0.6 3.0 8.5

IL-11† † † 0.1 207.1 0.0 3.6 16.9

IL-12 (p40)† † † 5.5 12,039.9 5.6 3.5 6.3

IL-12 (p70) 1.3 908.0 0.1 3.5 5.5

IL-19† † 8.5 6,223.8 0.2 3.8 8.4

IL-20† † † 2.7 5,809.9 3.6 2.3 11.1

IL-22 5.3 11,511.0 1.1 3.2 6.5

IL-26† 8.2 5,969.7 1.2 3.1 10.9

IL-27 (p28) 3.8 8,397.0 0.1 3.0 6.5

IL-28A/IFN-l2 10.7 7,813.5 1.8 4.3 7.9

IL-29/IFN-l1† † † 5.2 11,431.4 1.6 4.4 3.9

IL-32† 3.9 8,586.5 12.3 2.5 8.8

IL-34† † 61.9 45,142.7 51.9 3.2 6.7

IL-35† 20.6 45,037.6 3.7 2.6 9.1

continues


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* The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for a 10% intra-assay CV and recovery range of 70–130%. Data were generated using the magnetic workflow with the Bio-Plex Pro Wash Station.

† Of the top Luminex partners, Bio-Rad is the exclusive provider of this assay.† † Assay not available from Vendor M.† † † Assay not available from Vendor R.

Performance CharacteristicsAssay Working Range,

pg/ml*Assay Sensitivity,

pg/ml* Assay Precision*

Analyte LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV

LIGHT/TNFSF14† † † 14.8 3,585.3 10.2 3.1 10.4

MMP-1 106.8 233,460.8 33.7 4.8 9.0

MMP-2 1,224.5 297,540.2 39.0 4.8 7.7

MMP-3 395.7 288,429.4 28.5 3.7 8.8

Osteocalcin† † † 285.6 208,184.6 23.4 1.6 5.8

Osteopontin 115.3 252,154.4 91.3 2.3 4.5

Pentraxin-3 6.0 13,186.4 0.8 2.7 6.8

sTNF-R1 26.8 58,575.9 0.2 2.2 6.1

sTNF-R2 30.3 66,190.7 3.2 3.0 7.7

TSLP† † † 3.0 2,165.0 0.8 3.3 9.4

TWEAK / TNFSF12† † † 3.1 6,772.8 0.5 2.2 15.2



12

Bio-Plex Pro Human MMP and TIMP AssaysBio-Plex Pro Human MMP Panel 9-Plex (171AM001M)


MMP-1 171BM001M 43

MMP-2 171BM002M 26

MMP-3 171BM003M 45

MMP-7 171BM004M 66

MMP-8 171BM005M 37

MMP-9 171BM006M 55

MMP-10 171BM007M 62

MMP-12 171BM008M 53

MMP-13 171BM009M 47

Bio-Plex Pro Human TIMP Panel 4-Plex (171AM002M)

Analyte Bead Region

TIMP-1 21

TIMP-2 64

TIMP-3 52

TIMP-4 35

bio-rad.com/bio-plexmmpsandtimpsBio-Rad bulletin 6551

Performance Characteristics

Assay Working Ranges, pg/ml Assay Sensitivity, pg/ml

Analyte LLOQ ULOQ LOD

Human MMP Panel 9-Plex

MMP-1 51 111,152 35

MMP-2 621 1,357,988 450

MMP-3 130 221,609 116

MMP-7 21 44,953 5.4

MMP-8 30 66,000 1.5

MMP-9 138 302,498 24

MMP-10 69 83,333 1.6

MMP-12 12 25,655 1

MMP-13 17 38,240 4.9

Human TIMP Panel 4-Plex

TIMP-1 18.3 19,259 1.6

TIMP-2 17.9 39,038 2.4

TIMP-3 228.7 13,235 96.3

TIMP-4 6.9 15,000 1.7

Bio-Plex Pro RBM Human MMP and TIMP AssaysBio-Plex Pro Human MMP and TIMP Assays include the only Luminex panel on the market enabling the investigation nine MMPs in one sample at a single dilution.

http://www.bio-rad.com/en-us/product/bio-plex-pro-human-chemokine-assays

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Bio-Plex Pro Human Th17 Cytokinebio-rad.com/CCGFassays


The Bio-Plex Pro Human Th17 Cytokine Assays are a unique blend of magnetic bead–based immunoassays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammatory conditions.

Performance CharacteristicsHuman Th17 Analytes

Assay Working Assay Sensitivity, Assay Calibration to Range, pg/ml pg/ml Precision WHO/NIBSC

Alternate Intra-Assay Inter-Assay NIBSC Target Names LLOQ ULOQ LOD %CV %CV Factor** Catalog #

IFN-g — 2.54 20,779 0.43 3.3 10.1 1.7 87/586

IL-1b — 0.24 3,994 0.02 3.5 3.8 1.0 86/680

IL-4 — 1.33 1,361 0.52 2.4 8.6 0.7 88/656

IL-6 — 1.65 27,078 0.67 2.9 7.9 1.6 89/548

IL-10 — 1.99 32,674 0.30 2.8 6.2 1.3 93/722

IL-17A IL-17, CTLA8 1.20 19,682 0.49 2.2 6.2 0.4 01/420

IL-17A/F* — 1.62 6,631 1.28 4.3 5.8 — —

IL-17F — 3.04 18,668 0.80 4.3 8.2 — —

IL-21 Za11 8.97 147,023 2.13 3.3 6.7 — —

IL-22 — 3.88 11,917 0.30 2.2 5.7 — —

IL-23 (p19) IL-23A, SGRF 7.35 120,389 1.55 3.8 9.3 — —

IL-25 IL-17E 1.00 16,375 0.07 2.9 5.0 — —

IL-31 — 3.87 63,397 0.49 3.9 5.5 — —

IL-33 — 4.18 68,483 0.58 2.7 5.3 — —

sCD-40L CD154 2.41 39,457 0.41 2.8 8.7 — —

TNF-a — 0.57 9,270 0.07 4.1 5.1 0.4 88/786

* IL-17A/F values are derived from the singleplex assay format.** Factor × NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of Bio-Plex

standards (in the linear range) relative to NIBSC standards run on the same assay plate.

Bio-Plex Pro Human Th17 Analytes

Th17 Cytokine 15-Plex Bead Analyte (171AA001M) Singleplex Region

IFN-g • 171BA013M 21

IL-1b • 171BA001M 39

lL-4 • 171BA002M 52

lL-6 • 171BA003M 19

IL-10 • 171BA004M1 56

IL-17A • 171BA005M 76

IL-17A/F 171BA016M 66

IL-17F • 171BA006M 44

IL-21 • 171BA007M 47

IL-22 • 171BA008M 18

IL-23 (p19) • 171BA009M 43

IL-25 / IL-17E • 171BA010M 55

IL-31 • 171BA011M 62

IL-33 • 171BA012M 46

sCD40L • 171BA014M 29

TNF-a • 171BA015M 36



14

Bio-Plex Pro Human Chemokine AssaysBio-Plex Pro Human Chemokine Panel, 40-Plex (171AK99MR2)


6Ckine / CCL21 171BK11MR2 12

BCA-1 / CXCL13 171BK12MR2 74

CTACK / CCL27 171BK13MR2 72

ENA-78 / CXCL5 171BK14MR2 73

Eotaxin / CCL11 171BK15MR2 43

Eotaxin-2 / CCL24 171BK16MR2 30

Eotaxin-3 / CCL26 171BK17MR2 65

Fractalkine / CX3CL1 171BK18MR2 77

GCP-2 / CXCL6 171BK19MR2 15

GM-CSF 171BK21MR2 34

Gro-a / CXCL1 171BK22MR2 61 Gro-b / CXCL2 171BK23MR2 78

I-309 / CCL1 171BK24MR2 20

IFN-g 171BK25MR2 21

IL-1b 171BK26MR2 39

IL-2 171BK27MR2 38

IL-4 171BK28MR2 52

IL-6 171BK29MR2 19

IL-8 / CXCL8 / CXCL8 171BK31MR2 54

IL-10 171BK32MR2 56

IL-16 171BK33MR2 27

IP-10 / CXCL10 171BK34MR2 48

I-TAC / CXCL11 171BK35MR2 25


MCP-1 / CCL2 171BK36MR2 53

MCP-2 / CCL8 171BK37MR2 57

MCP-3 / CCL7 171BK38MR2 26

MCP-4 / CCL13 171BK39MR2 28

MDC / CCL22 171BK41MR2 29

MIF 171BK42MR2 35

MIG / CXCL9 171BK43MR2 14

MIP-1a / CCL3 171BK44MR2 55

MIP-1d / CCL15 171BK46MR2 66

MIP-3a / CCL20 171BK47MR2 62

MIP-3b / CCL19 171BK48MR2 76

MPIF-1 / CCL23 171BK49MR2 37

SCYB16 / CXCL16 171BK51MR2 64

SDF-1a+b / CXCL12 171BK52MR2 22

TARC / CCL17 171BK53MR2 67

TECK / CCL25 171BK54MR2 46

TNF-a 171BK55MR2 36

bio-rad.com/chemokineassaysBio-Rad bulletin 6499

Human Chemokines

Assay Working Ranges, pg/mlAssay

Sensitivity Assay Precision Calibration to WHO/NIBSC

Analyte LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV Factor* Catalog #

6Ckine / CCL21 21.9 3,923 12.0 4 6 — —

BCA-1 / CXCL13 0.7 1,200 0.1 2 2 — —

CTACK / CCL27 1.2 5,000 0.3 3 5 — —

ENA-78 / CXCL5 7.3 120,000 5.7 3 6 — —

Eotaxin / CCL11 1.5 3,859 0.7 3 4 — —

Eotaxin-2 / CCL24 6.2 4,073 3.2 3 4 — —

Eotaxin-3 / CCL26 0.9 12,109 0.5 3 4 — —

Fractalkine / CX3CL1 4.0 11,463 0.9 3 4 — —


continues

This panel consists of 40 assays to measure chemokines and chemotaxis-relevant analytes. This panel features a 40-plex all-in-one kit ideal for screening studies as well as custom configurations.


Bulletin 6335

15

* Factor x NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of the Bio-Plex standards (in the linear range) relative to NIBSC standards run on the same assay plate.

Human Chemokines

Assay Working Ranges, pg/mlAssay

Sensitivity Assay Precision Calibration to WHO/NIBSC

Analyte LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV Factor* Catalog #

GCP-2 / CXCL6 0.8 11,135 0.6 3 7 — —

GM-CSF 5.3 35,000 1.0 4 3 — —

Gro-a / CXCL1 3.1 7,024 4.2 2 4 — —

Gro-b / CXCL2 4.6 13,257 2.7 3 8 — —

I-309 / CCL1 1.8 1,015 1.6 3 6 — —

IFN-g 2.3 20,236 0.4 3 5 0.7 87/586

IL-1b 0.4 7,000 0.1 2 4 1.1 86/680

IL-2 0.8 13,000 0.1 3 6 — —

IL-4 1.2 4,804 1.0 2 4 1.2 88/656

IL-6 0.7 12,000 0.1 2 5 1.0 89/548

IL-8 / CXCL8 0.5 7,640 0.04 3 4 1.7 89/520

IL-10 1.3 18,708 0.9 3 3 1.8 93/722

IL-16 2.1 34,000 0.8 3 4 — —

IP-10 / CXCL10 1.6 7,714 1.1 2 7 — —

I-TAC / CXCL11 0.1 2,298 0.05 3 8 — —

MCP-1 / CCL2 0.3 4,812 0.1 3 3 2.5 92/794

MCP-2 / CCL8 0.3 4,056 0.04 3 5 — —

MCP-3 / CCL7 1.9 20,133 1.3 6 6 — —

MCP-4 / CCL13 0.2 3,368 0.1 3 4 — —

MDC / CCL22 0.9 14,649 0.5 3 3 — —

MIF 23.1 377,724 15.4 2 7 — —

MIG / CXCL9 1.8 19,600 1.1 3 7 — —

MIP-1a / CCL3 0.4 1,543 0.3 4 6 1.9 92/518

MIP-1d / CCL15 1.7 9,100 0.2 3 5 — —

MIP-3a / CCL20 0.3 4,675 0.1 2 3 — —

MIP-3b / CCL19 3.0 48,494 1.1 2 4 — —

MPIF-1 / CCL23 1.0 14,450 0.5 3 3 — —

SCYB16 / CXCL16 0.5 2,867 0.1 3 4 — —

SDF-1a+b / CXCL12 8.3 115,730 10.3 4 8 — —

TARC / CCL17 1.7 430 1.1 4 5 — —

TECK / CCL25 20.6 114,493 4.9 4 5 — —

TNF-a 0.9 13,879 0.2 3 8 1.8 88/786


Bio-Plex Pro Human Chemokine Assaysbio-rad.com/chemokineassays




16

Bio-Plex Pro TGF-b AssaysBio-Plex Pro TGF-b 3-Plex Panel (171W4001M)


TGF-b1 171V4001M 13

TGF-b2 171V4002M 72

TGF-b3 171V4003M 66

Performance CharacteristicsHuman TGF-b 3-Plex Panel

Assay Working Range, Assay Sensitivity, pg/ml pg/ml Assay Precision


Premixed 3-Plex Assay

TGF-b1 1.69 27,616 3.9 4.5 4.9

TGF-b2 14.7 30,080 1.9 6.3 9.1

TGF-b3 2.8 15,031 0.5 6.9 8.2


Bio-Plex Pro TGF-b Assays are magnetic bead–based multiplex assays designed to measure levels of TGF-b1, TGF-b2, and TGF-b3 in human, mouse, and rat models in diverse matrices like serum, plasma, urine, and human milk.


Bulletin 6335

17

Bio-Plex Pro Human Cancer Biomarker PanelsBio-Plex Pro Human Cancer Biomarker Panel 1 16-plex (171AC500M)

Receptors, cytokines, chemokines, growth factors, and hormones


sEGFR 171BC501M 15

FGF-basic 171BC502M 44

Follistatin 171BC503M 26

G-CSF 171BC504M 57

HGF 171BC505M 62

sHER-2/neu 171BC506M 12

sIL-6Ra 171BC507M 19

Leptin 171BC508M 78

Osteopontin 171BC509M 77

PECAM-1 171BC510M 47

PDGF-AB/BB 171BC511M 46

Prolactin 171BC512M 52

SCF 171BC513M 65

sTIE-2 171BC514M 64

sVEGFR-1 171BC515M 76

sVEGFR-2 171BC516M 45

Performance CharacteristicsHuman Cancer Biomarker Panel 1 — Receptors, cytokines, chemokines, growth factors, and hormones


Alternate Intra-Assay Inter-Assay NIBSC Target Names LLOQ ULOQ LOD %CV %CV Factor* Catalog #

sEGFR ErbB1, EGFR1 11.9 195,000 3.5 3.6 3.8 — —

FGF-basic FGF2, BFGF 10.2 4,500 5.1 2.9 4.7 6.6 90/712

Follistatin FSN 4.6 42,750 1.5 3.1 3.6 — —

G-CSF — 1.1 18,000 1.1 3.7 7.7 0.8 09/136

HGF SF 4.5 42,124 1.8 3.2 6.1 0.7 96/564

sHER-2/neu ErbB2 1.4 23,500 0.5 3.2 3.9 — —

sIL-6Ra CD126 1.4 12,985 0.5 3.1 5.3 — —

Leptin — 7.3 119,429 1.1 2.8 9.6 1.2 97/594

Osteopontin OPN 72.5 216,000 56.4 4.0 7.8 — —

PECAM-1 CD31 51.5 135,000 24.4 4.2 5.9 —

PDGF-AB/BB — 2.1 35,000 0.7 3.2 7.9 1.8 94/728

Prolactin PRL 12.2 200,000 7.0 3.0 6.8 1.5 98/582

SCF KL1 2.0 32,000 0.1 2.9 3.6 1.3 91/682

sTIE-2 CD202b 12.2 200,000 6.6 3.0 5.8 — —

sVEGFR-1 FLT1 4.3 35,324 0.8 2.9 3.3 — —

sVEGFR-2 KDR, FLK1 12.1 197,681 7.4 3.1 3.5 — —

* Factor × NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of Bio-Plex standards (in the linear range) relative to NIBSC standards run on the same assay plate.

bio-rad.com/bio-plexprocancerBio-Rad bulletin 6159

Bio-Plex Pro Human Cancer Biomarker Assays are a unique blend of magnetic bead–based assays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 34 cancer biomarkers involved in disease processes such as angiogenesis, metastasis, cell proliferation, cell adhesion/migration, apoptosis, and inflammation. The Bio-Plex Pro Human Cancer Biomarker Assays are available in two panels:

Panel 1 consists of receptors, cytokines, chemokines, growth factors, and hormones, and is available as a premixed 16-plex all-in-one kit. Panel 2 consists of ligands for panel 1 receptors, cytokines, chemokines, and growth factors, and is available as a premixed 18-plex all-in-one kit.



18

* Factor × NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of Bio-Plex standards (in the linear range) relative to NIBSC standards run on the same assay plate.

Bio-Plex Pro Human Cancer Biomarker PanelsPerformance CharacteristicsHuman Cancer Biomarker Panel 2 — Ligands for receptors in panel 1, cytokines, chemokines, and growth factors


Alternate Intra-Assay Inter-Assay NIBSC Target Names LLOQ ULOQ LOD %CV %CV Factor* Catalog #

Angiopoietin-2 19.5 51,100 3.3 2.4 6.1 — —

sCD40L TRAP 1.4 18,405 0.3 2.0 4.1 — —

EGF 0.3 4,942 0.1 4.1 7.2 0.6 91/530

Endoglin CD105 3.3 54,740 1.0 2.9 9.6 — —

sFASL CD95L/APO-1L 7.5 13,455 2.7 4.4 8.2 — —

HB-EGF 0.3 4,314 0.1 3.8 9.0 — —

IGFBP-1 4.2 39,514 1.5 3.2 4.7 — —

IL-6 0.7 11,489 0.1 2.7 6.4 1.7 89/548

IL-8 / CXCL8 CXCL8 0.4 4,943 0.1 3.0 4.9 1.0 89/520

IL-18 1.6 25,798 0.3 1.8 7.2 1.8 03/200

PAI-1 SERPINE1 3.7 59,826 0.2 2.5 5.9 — —

PLGF PGF 0.6 9,590 0.2 2.5 6.8 0.4 09/272

TGF-a 0.6 7,858 0.2 2.7 8.2 — —

TNF-a 0.6 9,572 0.2 3.4 6.3 0.4 88/786

uPA Urokinase 0.9 14,934 0.1 3.5 4.6 — —

VEGF-A VPF 1.7 27,518 0.4 2.4 8.6 1.1 02/286

VEGF-C Flt4 ligand 3.6 58,521 2.3 3.4 3.0 — —

VEGF-D FIGF 14.0 17,614 11.5 3.5 8.2 — —

Bio-Plex Pro Human Cancer Biomarker Panel 2 18-plex (171AC600M)

Ligands for receptors in panel 1, cytokines, chemokines, and growth factors


Angiopoietin-2 171BC601M 47

sCD40L 171BC602M 29

EGF 171BC603M 14

Endoglin 171BC604M 26

sFASL 171BC605M 35

HB-EGF 171BC606M 57

IGFBP-1 171BC607M 62

IL-6 171BC608M 19

IL-8 / CXCL8 171BC609M 54

IL-18* — 42

PAI-1 171BC611M 61

PLGF 171BC612M 52

TGF-a 171BC613M 76

TNF-a 171BC614M 36

uPA 171BC615M 78

VEGF-A 171BC616M 45

VEGF-C 171BC617M 64

VEGF-D 171BC618M 43

* IL-18 is not available in singleplex format.

bio-rad.com/bio-plexprocancerBio-Rad bulletin 6159


Bulletin 6335

19

continues

Bio-Plex Pro Human Cell Signaling Assays bio-rad.com/cellsignalingassaysBio-Rad bulletin 6285

Bio-Plex Pro Cell Signaling Phosphoprotein Assays

Lysate Controls Lysate Controls for Total Protein for Total Protein Phosphoprotein Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat. Bead Region

Akt (Ser473) 171V50001M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002 75

Akt (Thr308) 171V50002M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002 75

ATF-2 (Thr71) 171V50024M UV-treated HEK-293 171YZ0009 — — — 20

BAD (Ser136) 171V50025M PDGF-treated NIH3T3 171YZ0007 — — — 26

Btk (Tyr223) 171V50026M H2O2-treated Ramos 171YZ0011 171V60012M H2O2-treated Ramos 171YZ0011 39

c-AbI (Tyr245) 171V50027M Untreated K-562 171YZT003 — — — 45

c-Jun (Ser63) 171V50003M UV-treated HEK-293 171YZ0009 171V60002M Untreated HEK-293 171YZT001 56

CREB (Ser133) 171V50028M UV-treated HEK-293 171YZ0009 171V60013M Untreated HEK-293 171YZT001 19

EGFR (Tyr1068) 171V50004M EGF-treated HeLa 171YZ0002 — — — 44

EGFR (Tyr1173) 171V50005M EGF-treated HeLa 171YZ0002 — — — 44

Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) 171V50006M EGF-treated HEK-293 171YZ0001 171V60003M Untreated HeLa 171YZT002 38

GSK-3a/b (Ser21/Ser9) 171V50007M EGF-treated HEK-293 171YZ0001 171V60004M Untreated HeLa 171YZT002 18

HER-2 (Tyr1248) 171V50008M EGF-treated SK-Br3 171YZ0003 171V60005M EGF-treated SK-Br3 171YZ0003 30

HSP27 (Ser78) 171V50029M EGF-treated SK-Br3 171YZ0003 — — — 51

IGF-IR (Tyr1131) 171V50009M IGF-1-treated HEK-293 171YZ0005 171V60014M Untreated HEK-293 171YZT001 43

IR-b (Tyr1146) 171V50031M TNF-a-treated HeLa 171YZ0008 — — — 43

IRS-1 (Ser636/Ser639) 171V50030M PDGF-treated NIH3T3 171YZ0007 — — — 76

IkB-a (Ser32/Ser36) 171V50010M TNF-a-treated HeLa 171YZ0008 171V0006M Untreated HeLa 171YZT002 67

JNK (Thr183/Tyr185) 171V50011M UV-treated HEK-293 171YZ0009 171V0007M Untreated HeLa 171YZT002 34

Lyn (Tyr507) 171V50032M H2O2-treated Ramos 171YZ0011 — — — 33

MEK1 (Ser217/Ser221) 171V50012M EGF-treated HEK-293 171YZ0001 171V0008M Untreated HeLa 171YZT002 27

mTOR (Ser2448) 171V50033M PDGF-treated NIH3T3 171YZ0007 171V60015M Untreated HeLa 171YZT002 46

NF-kB p65 (Ser536) 171V50013M TNF-a-treated HeLa 171YZ0008 — — — 37

p38 MAPK (Thr180/Tyr182) 171V50014M UV-treated HEK-293 171YZ0009 171V60009M Untreated HeLa 171YZT002 36

p53 (Ser15) 171V50034M UV-treated HEK-293 171YZ0009 — — — 53

p70 S6 Kinase (Thr389) 171V50015M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002 55

p70 S6 Kinase (Thr421/Ser424) 171V50016M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002 55

p90 RSK (Ser380) 171V50035M EGF-treated SK-Br3 171YZ0003 — — — 35

http://www.bio-rad.com/evportal/destination/product?catID=cd39210b-08fa-44d0-acca-eab053201f19&source_wt=cellsignalingassays_surl


20



Lysate Controls Lysate Controls for Total Protein for Total Protein Phosphoprotein Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat. Bead Region

PDGFR-a (Tyr754) 171V50017M PDGF-treated NIH3T3 171YZ0007 — — — 28

PDGFR-b (Tyr751) 171V50018M PDGF-treated NIH3T3 171YZ0007 — — — 57

Pl3K p85 (Tyr458) 171V50036M H2O2-treated Ramos 171YZ0011 — — — 54

PTEN (Ser380) 171V50037M PDGF-treated NIH3T3 171YZ0007 171V60016M Untreated HeLa 171YZT002 22

S6 ribosomal protein (Ser235/Ser236) 171V50038M EGF-treated SK-Br3 171YZ0003 — — — 74

Smad2 (Ser465/Ser467) 171V50019M TNF-a-treated HeLa 171YZ0008 171V60011M Untreated HeLa 171YZT002 14

Src (Tyr416) 171V50039M Src-transfected NIH3T3 171YZ0013 171V60017M Src-transfected NIH3T3 171YZ0013 42

Stat1 (Tyr701) 171V50020M IFN-a-treated HeLa 171YZ0004 — — — 61

Stat3 (Ser727) 171V50021M IFN-a-treated HeLa 171YZ0004 — — — 52

Stat3 (Tyr705) 171V50022M IFN-a-treated HeLa 171YZ0004 — — — 52

Syk (Tyr352) 171V50040M H2O2-treated Ramos 171YZ0011 — — — 65

VEGFR-2 (Tyr1175) 171V50023M VEGF-treated HUVEC 171YZ0010 — — — 29

ZAP-70 (Tyr319) 171V50041M H2O2-treated Jurkat 171YZ0012 171V60018M H2O2-treated Jurkat 171YZ0012 64

Bio-Plex Pro Cell Signaling Akt Panel 8-Plex (LQ-00006JK0K0RR)

Analyte

Akt (Ser473) mTOR (Ser2448)

BAD (Ser136) p70 S6 Kinase (Thr389)

GSK-3a/b (Ser21/Ser9) PTEN (Ser380)

IRS-1 (Ser636/Ser639) S6 ribosomal protein (Ser235/Ser236)

Housekeeping Protein Singleplex Cat. Lysate Control Catalog

Human GAPDH 171V60019M Untreated HeLa 171YZT002

b-Actin 171V60020M Untreated HeLa 171YZT002

Negative control for all housekeeping — Detection antibody diluent* — protein assays

Negative Controls Lysate Control Catalog

Negative control for all phosphoprotein assays Phosphatase-treated HeLa 171YZB001

Negative control for all total target assays Detection antibody diluent* —

Bio-Plex Pro Cell Signaling MAPK Panel 9-plex (LQ-00000S6KL81S)

Analyte

ATF2 (Thr71) p38 (Thr180/Tyr182)

ERK1/2 (Thr202/Tyr204 Thr185/Tyr187) p53 (Ser15)

HSP27 (Ser78) p90 RSK (Ser380)

JNK (Thr183/Tyr185) Stat3 (Ser727)

MEK1 (Ser217/Ser221)

* Detection antibody diluent is included with the Bio-Plex Pro Cell Signaling Reagent Kit (cat #171304006M).


Bulletin 6335

21



Phosphoprotein Assays P/B Ratio* Intra-Assay %CV Inter-Assay %CVSensitivity (LOD),

µg/well Reaction Species**

Akt (Ser473) 65 2.9 4.5 0.15 H, M, R

Akt (Thr308) 34 3.3 6.1 0.15 H, M, R

ATF-2 (Thr71) 68 4.0 6.3 0.15 H, M, R, NHP

BAD (Ser136) 12 9.7 9.4 0.15 H, M, R, NHP

Btk (Tyr223) 99 3.2 1.3 0.15 H

c-AbI (Tyr245) 210 1.3 10.0 0.15 H, M

c-Jun (Ser63) 295 5.8 8.4 0.15 H, M, R

CREB (Ser133) 50 4.0 8.9 0.15 H, M, R

EGFR (Tyr1068) 98 14.1 17.7 0.15 H

EGFR (Tyr1173) 30 3.9 6.3 0.15 H

Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)

48 4.3 4.9 0.15 H, M

GSK-3a/b (Ser21/Ser9) 19 2.9 5.4 0.15 H, M, R

HER-2 (Tyr1248) 49 10.1 9.1 0.15 H, M, R

HSP27 (Ser78) 58 2.7 4.4 0.15 H, NHP

IGF-IR (Tyr1131) 68 4.8 6.5 0.15 H

IR-b (Tyr1146) 74 1.5 3.2 0.15 H, M, R

IRS-1 (Ser636/Ser639) 52 5.5 9.3 0.3 H, M, R

IkB-a (Ser32/Ser36) 103 6.8 8.9 0.15 H

JNK (Thr183/Tyr185) 73 4.7 4.1 0.15 H

Lyn (Tyr507) 125 4.2 4.4 0.15 H, M, R

MEK1 (Ser217/Ser221) 44 1.9 5.5 0.15 H, M, R

mTOR (Ser2448) 40 3.3 9.2 0.3 H, M, R, NHP

NF-kB p65 (Ser536) 20 10.7 13.5 0.3 H, M, R

p38 MAPK (Thr180/Tyr182) 62 3.1 6.4 0.15 H, M, R

p53 (Ser15) 59 8.0 12.2 0.15 H

p70 S6 Kinase (Thr389) 196 8.4 10.3 0.15 H, M, R

p70 S6 Kinase (Thr421/Ser424)

11 2.9 20.1 0.15 H, M, R

p90 RSK (Ser380) 80 1.7 4.2 0.15 H, M, R, NHP

PDGFR-a (Tyr754) 40 5.0 6.2 0.04 H, M

PDGFR-b (Tyr751) 142 8.1 10.3 0.04 H, M, R

Pl3K p85 (Tyr458) 42 9.3 11.4 1.6 H, M, R

PTEN (Ser380) 39 9.0 6.3 0.3 H, M, R

continues

The capacity for multiplexed biomarker analysis makes Bio-Plex the ideal platform for signal transduction assays. Bio-Plex Pro Cell Signaling Assays can be used to determine intracellular levels of phosphorylated proteins in the investigation of diverse biological processes including apoptosis, translation control, gene expression, cell cycle regulation, cytoskeletal restructuring, and neurological function.



22


Phosphoprotein Assays P/B Ratio* Intra-Assay %CV Inter-Assay %CVSensitivity (LOD),

µg/well Reaction Species**

S6 Ribosomal Protein (Ser235/Ser236)

26 4.3 5.2 0.15 H, M, R

Smad2 (Ser465/Ser467) 14 4.4 8.8 0.3 H, M, R

Src (Tyr416) 178 5.7 9.1 0.15 H, M, R

Stat1 (Tyr701) 18 3.6 7.8 0.15 H

Stat3 (Ser727) 89 7.0 10.1 0.15 H, M, R

Stat3 (Tyr705) 18 2.5 6.8 0.15 H, M, R

Syk (Tyr352) 232 1.4 2.0 0.15 H, M, R

VEGFR-2 (Tyr1175) 614 8.3 11.7 0.15 H, M

ZAP-70 (Tyr319) 138 2.6 5.1 0.15 H, M


Performance CharacteristicsTotal Target Assays Intra-Assay %CV Inter-Assay %CV Sensitivity (LOD), µg/well Reaction Species*

b-Actin 3.4 13.0 0.15 H, M, R, NHP

Akt 3.8 8.4 0.15 H, M, R

Btk 7.6 10.0 0.15 H, M

c-Jun 4.0 4.7 0.15 H

CREB 0.7 10.4 0.15 H, M, R, NHP

Erk1/2 3.4 9.2 0.15 H, M

GAPDH 1.9 4.8 0.15 H

GSK-3b 2.9 4.1 0.8 H, M, R

HER-2 5.6 4.2 0.04 H, M, R

IGF-IR 4.7 4.4 0.15 H

IkB-a 1.9 3.1 0.15 H

JNK 1.9 4.5 0.3 H

MEK 1 1.8 3.9 0.15 H, M, R

mTOR 4.1 3.3 0.15 H, M, R, NHP

p38 MAPK 4.3 9.6 0.3 H, M, R

p70 S6 Kinase 2.0 3.6 0.15 H, M, R

PTEN 5.0 3.2 0.15 H, M, R

Smad2 3.9 16.9 0.15 H

Src 11.3 5.4 0.15 H, M, R

ZAP-70 3.1 11.2 0.15 H

* H, human; M, mouse; R, rat; NHP, non-human primate.

* Signal ratio (positive/background) of the stimulated cell lysate divided by the negative control cell lysate. ** H, human; M, mouse; R, rat; NHP, non-human primate.


Bulletin 6335

23

Bio-Plex Pro Human RBM Apoptosis AssaysBio-Plex Pro RBM Apoptosis Panel 1 (171WAR1CK)

Analyte Bead Region

Bak 74

Bax 27

Lamin B, intact and 45 kD 14

Smac 19

Bio-Plex Pro RBM Apoptosis Panel 2 (171WAR2CK)

Analyte Bead Region

Bad 73

Bax / Bcl-2 dimer 42

Bcl-xL 22

Bim 12

Mcl-1 18

Bio-Plex Pro RBM Apoptosis Panel 3 (171WAR3CK)

Analyte Bead Region

Bcl-xL / Bak dimer 47

Caspase-3, active 57

Mcl-1 / Bak dimer 54

Survivin 20

Performance CharacteristicsApoptosis

Assay Working Range, ng/ml

Assay Sensitivity, ng/ml Assay Precision

Target Bead Region LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV

Panel 1

Bak 74 0.43 630 0.18 6% 14%Bax 27 0.25 255 0.26 10% 19%Lamin B, intact and 45 kD 14 0.057 95 0.044 5% 14%Smac 19 0.16 165 0.075 8% 19%

Panel 2

Bad 73 0.27 200 0.18 5% 9%Bax/Bcl-2 dimer 42 0.47 1020 0.47 7% 9%Bcl-xL 22 0.070 30 0.046 4% 7%Bim 12 0.014 16 0.015 4% 8%Mcl-1 18 0.10 180 0.10 6% 6%

Panel 3

Bcl-xL/Bak dimer 47 0.081 45 0.021 5% 14%Caspase-3, active 57 0.039 50 0.023 6% 8%Mcl-1/Bak dimer 54 0.33 600 0.27 8% 13%Survivin 20 0.056 50 0.023 6% 8%

bio-rad.com/apoptosisassaysBio-Rad bulletin 6474

Use the Bio-Plex Pro RBM Apoptosis Multiplex Assays for the detection of a highly relevant set of intracellular proteins involved in the commitment, onset, and induction of apoptosis by the intrinsic pathway.



24

Bio-Plex Pro Human Diabetes Assays (compatible with non-human primate samples)

Bio-Plex Pro Human Diabetes 10-Plex Panel (171A7001M)


C-Peptide 171B7003M 72

Ghrelin 171B7004M 26

GIP 171B7005M 14

GLP-1 171B7006M 27

Glucagon 171B7007M 15

Insulin 171B7008M 12

Leptin 171B7009M 78

PAI-1 171B7010M 61

Resistin 171B7011M 65

Visfatin 171B7012M 22

Bio-Plex Pro Human Diabetes 2-Plex Panel (171A7002M)


Adiponectin* 171A7003M 64

Adipsin* 171A7004M 35

Bio-Plex Pro Human Diabetes Adipsin Assay All-in-One Kit (171A7004M)


Adipsin* 171A7004M 35

Bio-Plex Pro Human Diabetes Adiponectin Assay All-in-One Kit (171A7003M)


Adiponectin* 171A7003M 64

* Adiponectin and adipsin can also be assayed; they are available as either singleplexes or a 2-plex. Due to different dilution schemes, these biomarkers should be assayed separately from the ten markers in the panel.

Performance CharacteristicsHuman Diabetes


Target LLOQ ULOQ LOD Intra-Assay%CV Inter-Assay%CV

2-Plex Assay

Adiponectin 160 218,485 32.7 4 2

Adipsin 43 14,513 17.0 6 4

10-Plex Assay

C-Peptide 22.4 10,031 14.5 5 4

Ghrelin 16.6 8,502 1.2 4 2

GIP 11.2 22,895 0.8 3 4

GLP-1 31.3 16,000 5.3 6 3

Glucagon 15.7 3,500 4.9 5 6

Insulin 1.7 3,541 1.0 3 5

Leptin 11.5 129,107 3.1 3 4

PAI-1 8.8 47,850 2.2 5 4

Resistin 2.3 4,739 1.3 3 4

Visfatin 51.3 280,266 37.1 4 3

bio-rad.com/bio-plexprodiabetesBio-Rad bulletin 6342

The Bio-Plex Pro Human Diabetes Assays offer reliable performance and a convenient format for studying human diabetes and obesity markers. These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (<3 hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 μl of sample.


Bulletin 6335

25

Bio-Plex Pro Human Metabolic and Hormone AssaysBio-Plex Pro Human Metabolic Panel 1 (171AMR1CK)

Analyte Bead Region

C-Peptide 45

Cortisol 12

Pancreatic polypeptide 52

Proinsulin 67

Peptide YY 55

bio-rad.com/bio-plexmetabolicassaysBio-Rad bulletin 6571

Bio-Plex Pro Human Metabolic Panel 2 (171AMR2CK)

Analyte Bead Region

FGF-21 63

FGF-23 47

Galectin-3 46

sLeptin R 27

Omentin-1 20

Pentraxin-3 44

PON-1 53

Vaspin 34


Analyte Bead Region

AAT 26

AGP-1 57

Hemopexin 35

RBP-4 43

Transferrin 61

Transthyretin 13

VDBP 28


Analyte Bead Region

ANGPTL3 66

Chemerin 42

DPP4 25

Protein S 62

SEPP 36

Bio-Plex Pro Human Hormone Panel 1 (171AHR1CK)

Analyte Bead Region

FSH 18

GH 38

LH 15

Prolactin 19

TSH 22

Bio-Plex Pro Human IGFBP Panel (171AGR1CK)

Analyte Bead Region

IGFBP-1 48

IGFBP-2 65

IGFBP-3 33

IGFBP-4 76

IGFBP-5 51

IGFBP-6 78

IGFBP-7 37

Bio-Plex Pro Human IGF Panel (171AFR1CK)

Analyte Bead Region

IGF-1 21

IGF-2 54

Performance CharacteristicsAssay Working

RangesAssay

Sensitivity Assay Precision

Panel Analyte Alternative Names LLOQ ULOQ LOD Units Intra-Assay %CV Inter-Assay %CV

Human Metabolic Panel 1

C-Peptide Connecting peptide 2.1 2,100 0.92 pg/ml 2 5

Cortisol 2,877 107,873 298 pg/ml 11 16

Pancreatic polypeptide PP 1.6 940 0.44 pg/ml 3 5

Proinsulin 51 23,765 14 pg/ml 3 5

Peptide YY PYY 58 26,750 12 pg/ml 3 6

The Bio-Plex Pro RBM Human Metabolic and Hormone Assays, developed in partnership with Myriad RBM, comprise a highly relevant set of biomarkers involved in diabetes, obesity, metabolic syndrome, cardiovascular disease, and the hormonal control of metabolism and reproductive organs. The assays are built on magnetic beads to enable robust quantification of multiple proteins in human serum, plasma, and cell culture media samples. The assays are offered as premixed all-in-one kits.

continues



26

Performance CharacteristicsAssay Working

RangesAssay

Sensitivity Assay Precision

Panel Analyte Alternative Names LLOQ ULOQ LOD Units Intra-Assay %CV Inter-Assay %CV


FGF-21 Fibroblast growth factor 21 25 18,000 11 pg/ml 16 19

FGF-23 Fibroblast growth factor 23 24 12,000 5.8 pg/ml 10 16

Galectin-3 Galactoside-binding, soluble, 3 54 15,000 36 pg/ml 11 20

sLeptin R Soluble leptin receptor 382 495,000 321 pg/ml 8 9

Omentin-1 Intelectin-1 10,277 13,500,000 6,407 pg/ml 13 15

Pentraxin-3 PTX-3 18 22,000 14 pg/ml 7 12

PON-1 Serum paraoxonase-1 8,343 12,150,000 6,300 pg/ml 8 8

Vaspin Visceral adipose tissue-derived serine protease inhibitor

2.3 1,950 1.4 pg/ml 10 13

Human Metabolic Panel 3*

AAT Alpha-1 antitrypsin 0.22 90 0.04 ng/ml 6 7

AGP-1 Alpha-1-acid glycoprotein 0.0041 9 0.0037 ng/ml 7 11

Hemopexin 0.26 200 0.086 ng/ml 8 8

RBP-4 Retinol-binding protein-4 0.014 10 0.0076 ng/ml 6 9

Transferrin Serotransferrin 0.64 520 0.24 ng/ml 4 6

Transthyretin TTR 0.013 5 0.0014 ng/ml 6 6

VDBP Vitamin D-binding protein 0.17 90 0.061 ng/ml 4 7


ANGPTL3 Angiopoietin-like 3 0.018 40 0.018 ng/ml 8 10

Chemerin 0.026 12.8 0.015 ng/ml 5 9

DPP4 Dipeptidyl peptidase-4 0.033 22 0.012 ng/ml 7 8

Protein S Vitamin K-dependent protein S 0.061 80 0.037 ng/ml 8 9

SEPP Selenoprotein P 2.0 1,415 0.90 ng/ml 9 16

Human Hormone Panel 1

FSH Follicle-stimulating hormone 0.071 115 0.046 mIU/ml 7 8

GH Human growth hormone-1 0.023 20 0.0076 ng/ml 6 7

LH Luteinizing hormone 0.14 100 0.044 mIU/ml 4 6

Prolactin PRL 0.018 19 0.0077 ng/ml 7 8

TSH Thyroid-stimulating hormone 0.017 16 0.0044 µIU/ml 5 7

Human IGFBP Panel

IGFBP-1 Insulin-like growth factor binding proteins

0.014 25 0.011 ng/ml 4 7

IGFBP-2 1.2 750 0.41 ng/ml 6 12

IGFBP-3 1.2 1,020 0.96 ng/ml 4 13

IGFBP-4 0.24 370 0.17 ng/ml 4 11

IGFBP-5 1.3 187 0.23 ng/ml 5 14

IGFBP-6 0.63 200 0.37 ng/ml 7 11

IGFBP-7 0.53 165 0.32 ng/ml 5 9

Human IGF Panel

IGF-1 Insulin-like growth factor 0.25 87 0.10 ng/ml 2 6

IGF-2 0.19 35 0.13 ng/ml 2 4

* The product datasheet supplied with metabolic panel 3 provides performance values as well as standard S1 and control values in μg/ml. Values here are in ng/ml for display purposes.

bio-rad.com/bio-plexmetabolicassaysBio-Rad bulletin 6571


Bulletin 6335

27

Bio-Plex Pro Human Kidney Toxicity AssaysBio-Plex Pro RBM Human Kidney Toxicity Panel 1 (171ATR1CK)

Analyte Bead Region

Calbindin 64

Clusterin 12

GST-π 25

IL-18 21

KIM-1 44

MCP-1 / CCL2 15

Bio-Plex Pro RBM Human Kidney Toxicity Panel 2 (171ATR2CK)

Analyte Bead Region

Albumin 30

B2M 22

Cystatin C 51

NGAL 46

Osteopontin 20

TFF3 61

Performance CharacteristicsKidney Toxicity

Assay Working Assay Assay Precision Alternate Ranges, ng/ml Sensitivity, ng/ml Intra-Assay Inter-Assay Analyte Names LLOQ ULOQ LOD %CV %CV

Human Kidney Toxicity Panel 1

Calbindin 4.4 1,750 0.97 4 5 Clusterin ApoJ 1.3 1,250 0.57 3 10 GST-π 0.5 230 0.23 5 8 IL-18 0.019 15 0.048 5 7 KIM-1 TIM-1 0.021 21 0.01 4 5 MCP-1 / CCL2 0.011 3.8 0.0017 3 8

Human Kidney Toxicity Panel 2

Albumin 2.8 640 1.2 2 8 B2M 0.043 22 0.022 3 9 Cystatin C 0.16 40 0.077 3 20 NGAL Lipocalin-2 0.062 34 0.052 3 8 Osteopontin OPN 3.8 2,100 1.7 6 12 TFF3 0.075 98 0.036 2 6

bio-rad.com/bio-plexkidneytoxBio-Rad bulletin 6347

The Bio-Plex Pro RBM Human Kidney Toxicity Assays, developed in partnership with Myriad RBM, comprise a highly relevant set of biomarkers for early detection and characterization of kidney injury. Myriad RBM’s close collaboration with the Predictive Safety Testing Consortium (PSTC), FDA, and European Medicines Evaluation Agency (EMEA) was instrumental in the selection of the markers found in these panels. The assays are built on magnetic beads to enable robust quantification of multiple proteins in human urine samples, providing valuable information throughout drug development — from lead optimization to preclinical and clinical protocol decision making. The assays are offered as premixed all-in-one kits.



28

Bio-Plex Pro Human Isotyping AssaysBio-Plex Pro Human Isotyping Panel (171A3100M)

Analyte 6-Plex Singleplex Bead Region

IgA • 171A3101M 12

IgM • 171A3104M 56

IgE* — 171A3102M 38

IgG-total* — 171A3103M 66

IgG1 • — 54

IgG2 • — 21

IgG3 • — 36

IgG4 • — 72

* IgE and IgG-total are not part of the 6-plex.

Performance CharacteristicsHuman Isotyping



Singleplex Assay

lgE 0.02 406 0.0040 3 14

lgG total 3.00 30,270 2.1603 4 3

6-Plex Assay

lgG1 0.13 2,107 0.0264 2 4

lgG2 2.74 11,227 0.6615 4 5

lgG3 0.07 863 0.0030 3 5

lgG4 0.02 200 0.0021 4 5

lgA 0.13 532 0.1034 6 3

lgM 0.78 6,376 0.1473 3 4

bio-rad.com/bio-plexproisotypingBio-Rad bulletin 6344

The Bio-Plex Pro Human Isotyping Assays are available in a multiplex format and offer robust and reproducible measurement of eight different immunoglobulin subclasses or isotypes associated with allergic responses, immunodeficiency disorders, autoimmune diseases, cancer, infectious diseases, and drug and vaccine development.


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Bio-Plex Pro Human Acute Phase AssaysBio-Plex Pro Human Acute Phase 5 + 4-Plex Panel (171A4S07M)

Analyte Bead Region

CRP 81

Ferritin 38

Fibrinogen 52

Haptoglobin 79

a-2-macroglobin 92

Procalcitonin 56

SAA 96

SAP 98

tPA 21

Bio-Plex Pro Human Acute Phase 4-Plex Panel (171A4C09M)

Analyte Bead Region

CRP 81

Haptoglobin 79

a-2-macroglobin 92

SAP 98

Bio-Plex Pro Human Acute Phase 5-Plex Panel (171A4C07M)

Analyte Bead Region

Ferritin 38

Fibrinogen 52

Procalcitonin 56

SAA 96

tPA 21

The Bio-Plex Pro Human Acute Phase Assays use 8.0 µm magnetic beads, which are compatible with the Bio-Plex 200 and Luminex 200 readers, and are not compatible with the MAGPIX and 3D readers.

Performance CharacteristicsHuman Acute Phase

Assay Working Assay Target Range Sensitivity Dynamic WHO/NIBSC

LLOQ ULOQQ LOD Ranges* Catalog # 1 IU = x ng**

CRP 0.01 ng/ml 50 ng/ml 0.004 ng/ml 0.01–125 ng/ml 85/506 3.0 x 106

Ferritin 3.05 pg/ml 50,000 pg/ml 1.3 pg/ml 3.05–50,000 pg/ml — —

Fibrinogen 5 ng/ml 813 ng/ml 2.8 ng/ml 1.95–32,000 ng/ml — —

Haptoglobin 0.1 ng/ml 500 ng/ml 0.07 ng/ml 0.03–500 ng/ml — —

a-2-Macroglobulin 0.5 ng/ml 1,875 ng/ml 0.25 ng/ml 0.5–7,500 ng/ml — —

Procalcitonin 14 pg/ml 10,000 pg/ml 11.3 pg/ml 0.61–10,000 pg/ml — —

SAA 1 ng/ml 700 ng/ml 1.1 ng/ml 0.06–1,000 ng/ml — —

Serum amyloid P 0.1 ng/ml 250 ng/ml 0.063 ng/ml 0.02–250 ng/ml — — (SAP)

Tissue plasminogen 28 pg/ml 5,000 pg/ml 6.15 pg/ml 0.31–5,000 pg/ml 98/714 2.4 x 10–1 activator

* Standard curve ranges.** IU, international units.

bio-rad.com/bio-plexproacutephaseBio-Rad bulletin 5650

Disease research involving the study of inflammation, sepsis, cardiovascular disease, and diabetes often requires the measurement of multiple acute phase biomarkers. Bio-Plex Pro Human Acute Phase Assays deliver accurate and reproducible measurements of nine of the most commonly measured biomarkers.



30

Bio-Plex Pro Mouse Cytokine Group l AssaysBio-Plex Pro Mouse Cytokine 8-Plex Panel (M60000007A)


GM-CSF 171G5016M 73

IFN-g 171G5017M 34

IL-1b 171G5002M 19

IL-2 171G5003M 36

IL-4 171G5005M 39

IL-5 171G5006M 52

IL-10 171G5009M 56

TNF-a 171G5023M 21

Bio-Plex Pro Mouse Cytokine 23-Plex Panel (M60009RDPD)


Eotaxin 171G5014M 74

G-CSF 171G5015M 54

GM-CSF 171G5016M 73

IFN-g 171G5017M 34

IL-1a 171G5001M 53

IL-1b 171G5002M 19

IL-2 171G5003M 36

IL-3 171G5004M 18

IL-4 171G5005M 39

IL-5 171G5006M 52

IL-6 171G5007M 38

IL-9 171G5008M 33

IL-10 171G5009M 56

IL-12 (p40) 171G5010M 76

IL-12 (p70) 171G5011M 78

IL-13 171G5012M 37

IL-17A 171G5013M 72

KC 171G5018M 57

MCP-1 / CCL2 171G5019M 51

MIP-1a / CCL3 171G5020M 77

MIP-1b 171G5021M 75

RANTES 171G5022M 55

TNF-a 171G5023M 21

Bio-Plex Pro Mouse Cytokine Th1/Th2 Panel (M6000003J7)


GM-CSF 171G5016M 73

IFN-g 171G5017M 34

IL-2 171G5003M 36

IL-4 171G5005M 39

IL-5 171G5006M 52

IL-10 171G5009M 56

IL-12 (p70) 171G5011M 78

TNF-a 171G5023M 21

Bio-Plex Pro Mouse Cytokine Th1 Panel (L6000004C6)


IFN-g 171G5017M 34

IL-1b 171G5002M 19

IL-2 171G5003M 36

IL-6 171G5007M 38

IL-10 171G5009M 56

IL-12 (p70) 171G5011M 78

TNF-a 171G5023M 21

Bio-Plex Pro Mouse Cytokine Th2 Panel (L60000UKVT)


IL-2 171G5003M 36

IL-4 171G5005M 39

IL-5 171G5006M 52

IL-6 171G5007M 38

IL-9 171G5008M 33

IL-10 171G5009M 56

IL-13 171G5012M 37

Mouse Assays

Performance CharacteristicsMouse Cytokine Group I



Eotaxin 257.9 4,636 147.4 4 5

G-CSF 5.1 84,244 0.6 3 12

GM-CSF 21.2 3,401 5.6 3 24

IFN-g 1.84 30,164 1.2 4 6

IL-1a 1.84 21,093 0.2 3 29

IL-1b 10.36 60,631 9.4 4 7

IL-2 3.72 51,857 0.6 3 19

IL-3 1.55 21,632 0.2 2 15

IL-4 6.98 9,372 2.1 3 20

IL-5 3.57 13,315 0.3 4 14

IL-6 0.74 12,053 0.2 3 16

IL-9 6.89 28,208 12.5 4 9

IL-10 2.95 12,066 1.0 4 5

IL-12 (p40) 1.53 25,024 0.4 2 7

IL-12 (p70) 1.62 26,507 2.3 3 7

IL-13 47.2 57,011 38.7 4 5

IL-17A 2.65 43,337 0.8 3 10

KC 3.2 18,202 0.3 3 30

MCP-1 / CCL2 22.4 41,873 3.7 5 7

MIP-1a / CCL3 256.2 15,565 36.3 3 11

MIP-1b 3.33 24,798 0.8 3 8

RANTES 2.78 8,759 0.6 4 4

TNF-a 5.8 59,626 1.4 3 6


These assays are magnetic bead–based assays designed for the detection and measurement of 46 mouse cytokines, chemokines, and growth factors. They are available as premixed assay panels, singleplex sets, or as custom x-Plex and Express Assays.


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Bio-Plex Pro Mouse Cytokine Group ll, lll, and TGF-b AssaysBio-Plex Pro Mouse Cytokine Group ll 9-Plex Panel (MD000000EL)


FGF-basic 171G6002M 25

IL-15 171G6001M 42

IL-18** 20

LIF 171G6003M 45

M-CSF 171G6004M 26

MIG / CXCL9 171G6005M 44

MIP-2 171G6006M 27

PDGF-BB 171G6007M 35

VEGF 171G6008M 47

Bio-Plex Pro Mouse Cytokine Group lll Singleplex Sets


ICAM-1 171GA010M 22

IL-25 / IL-17E 171GA006M 67

IL-27 (p28) 171GA007M 43

Bio-Plex Pro Mouse Cytokine Group llI Th17 8-Plex Panel B (171FA001M)


sCD40L 171GA001M 30

IL-17F 171GA002M 28

IL-21 171GA003M 14

IL-22 171GA004M 15

IL-23 (p19) 171GA005M 61

IL-31 171GA008M 29

IL-33 171GA009M 13

MIP-3a / CCL20 171GA011M 12

Bio-Plex Pro TGF-b 3-Plex Panel (171W4001M)


TGF-b1 171V4001M 13

TGF-b2 171V4002M 72

TGF-b3 171V4003M 66

Performance CharacteristicsMouse Cytokine Group ll



FGF-basic 4.8 35,500 2.2 8 8

IL-15 5.7 37,501 6.6 6 3

IL-18 81.4 29,761 31.8 8 18

LIF 3.5 57,366 0.6 4 12

M-CSF 1.5 24,221 0.4 4 14

MIG / CXCL9 183 46,393 3.4 5 21

MIP-2 32 8,574.2 0.4 5 13

PDGF-BB 10.4 36,939 4.3 5 10

VEGF 27.2 32,771 1.6 4 15

Mouse Cytokine Group lll



sCD40L 3.6 11,245 0.4 4 14ICAM-1 2.9 23,723 0.78 4 8

IL-17F 1 6,362 0.1 5 5IL-21 6 8,207 2.8 3 15IL-22 0.5 8,433 0.1 4 5IL-23 (p19) 3.4 55,764 0.7 5 6

IL-25 / IL-17E 168 98,266 9.0 9 12IL-27 (p28) 63.4 117,521 29.2 9 6IL-31 2.5 41,313 5.2 6 6IL-33 0.5 7,343 0.2 5 12

MIP-3a / CCL20 0.7 1,660 0.3 6 13

Mouse TGF-b 3-Plex Panel



TGF-b1 1.69 27,616 3.9 4.5 4.9TGF-b2 14.7 30,080 1.9 6.3 9.1TGF-b3 2.8 15,031 0.5 6.9 8.2

bio-rad.com/CCGFassaysBio-Rad bulletins 5827, 6054, and 6110

Bio-Plex Pro Mouse Cytokine Th17 6-Plex Panel A (M6000007NY)


IFN-g 171G5017M 34

IL-1b 171G5002M 19

IL-6 171G5007M 38

IL-10 171G5009M 56

IL-17A 171G5013M 72

TNF-a 171G5023M 21

* For performance characteristics, please see page 30.



32

Bio-Plex Pro Mouse Cell Signaling Assays bio-rad.com/cellsignalingassaysBio-Rad bulletin 6285 Bio-Plex Pro Mouse Cell Signaling Phosphoprotein Assays

Lysate Controls Lysate Controls for Total Protein for Total Protein Phosphoprotein Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat.

Akt (Ser473) 171V50001M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002

Akt (Thr308) 171V50002M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002

ATF-2 (Thr71) 171V50024M UV-treated HEK-293 171YZ0009 — — —

BAD (Ser136) 171V50025M PDGF-treated NIH3T3 171YZ0007 — — —

c-AbI (Tyr245) 171V50027M Untreated K-562 171YZT003 — — —

c-Jun (Ser63) 171V50003M UV-treated HEK-293 171YZ0009 171V60002M Untreated HEK-293 171YZT001

CREB (Ser133) 171V50028M UV-treated HEK-293 171YZ0009 171V60013M Untreated HEK-293 171YZT001

Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) 171V50006M EGF-treated HEK-293 171YZ0001 171V60003M Untreated HeLa 171YZT002

GSK-3a/b (Ser21/Ser9) 171V50007M EGF-treated HEK-293 171YZ0001 171V60004M Untreated HeLa 171YZT002

HER-2 (Tyr1248) 171V50008M EGF-treated SK-Br3 171YZ0003 171V60005M EGF-treated SK-Br3 171YZ0003

IR-b (Tyr1146) 171V50031M TNF-a-treated HeLa 171YZ0008 — — —

IRS-1 (Ser636/Ser639) 171V50030M PDGF-treated NIH3T3 171YZ0007 — — —

Lyn (Tyr507) 171V50032M H2O2-treated Ramos 171YZ0011 — — —

MEK1 (Ser217/Ser221) 171V50012M EGF-treated HEK-293 171YZ0001 171V0008M Untreated HeLa 171YZT002

mTOR (Ser2448) 171V50033M PDGF-treated NIH3T3 171YZ0007 171V60015M Untreated HeLa 171YZT002

NF-kb p65 (Ser536) 171V50013M TNF-a-treated HeLa 171YZ0008 — — —

p38 MAPK (Thr180/Tyr182) 171V50014M UV-treated HEK-293 171YZ0009 171V60009M Untreated HeLa 171YZT002

p70 S6 Kinase (Thr389) 171V50015M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002

p70 S6 Kinase (Thr421/Ser424) 171V50016M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002

p90 RSK (Ser380) 171V50035M EGF-treated SK-Br3 171YZ0003 — — —

continues


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33

Bio-Plex Pro Mouse Cell Signaling Assays bio-rad.com/cellsignalingassaysBio-Rad bulletin 6285

Bio-Plex Pro Mouse Cell Signaling Phosphoprotein Assays

Lysate Controls Lysate Controls Total Protein for Total Protein Phosphoprotein for Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat.

PDGFR-a (Tyr754) 171V50017M PDGF-treated NIH3T3 171YZ0007 — — —

PDGFR-b (Tyr751) 171V50018M PDGF-treated NIH3T3 171YZ0007 — — —

Pl3K p85 (Tyr458) 171V50036M H2O2-treated Ramos 171YZ0011 — — —

PTEN (Ser380) 171V50037M PDGF-treated NIH3T3 171YZ0007 171V60016M Untreated HeLa 171YZT002

S6 ribosomal protein (Ser235/Ser236) 171V50038M EGF-treated SK-Br3 171YZ0003 — — —

Smad2 (Ser465/Ser467) 171V50019M TNF-a-treated HeLa 171YZ0008 171V60011M Untreated HeLa 171YZT002

Src (Tyr416) 171V50039M Src-transfected NIH3T3 171YZ0013 171V60017M Src-transfected NIH3T3 171YZ0013

Stat3 (Ser727) 171V50021M IFN-a-treated HeLa 171YZ0004 — — —

Stat3 (Tyr705) 171V50022M IFN-a-treated HeLa 171YZ0004 — — —

Syk (Tyr352) 171V50040M H2O2-treated Ramos 171YZ0011 — — —

VEGFR-2 (Tyr1175) 171V50023M VEGF-treated HUVEC 171YZ0010 — — —

ZAP-70 (Tyr319) 171V50041M H2O2-treated Jurkat 171YZ0012 171V60018M H2O2-treated Jurkat 171YZ0012

Bio-Plex Pro Mouse Cell Signaling Akt Panel 8-Plex (LQ-00006JK0K0RR)

Analyte












* See pages 21–22 for additional performance characteristics.



34 34

Bio-Plex Pro Mouse Diabetes AssaysBio-Plex Pro Mouse Diabetes 8-Plex (171F7001M)


Ghrelin 171G7002M 64

GIP 171G7003M 46

GLP-1 171G7004M 62

Glucagon 171G7005M 63

Insulin 171G7006M 66

Leptin 171G7007M 65

PAI-1 171G7008M 48

Resistin 171G7009M 30

Bio-Plex Pro Mouse Diabetes Assays Adiponectin All-in-One (171F7002M)


Adiponectin 171F7002M 29

Mouse Diabetes

Assay Working Ranges, Assay Sensitivity, pg/ml pg/ml Assay Precision


Singleplex Assay

Adiponectin* 38.0 62,043 8.4 4 3

8-Plex Assay

Ghrelin 3.1 7,296 0.8 5 4

GIP 13.4 14,999 2.3 4 10

GLP-1 3.4 1,969 0.8 6 11

Glucagon 24.0 3,067 7.0 6 6

Insulin 93.4 47,815 22.0 6 4

Leptin 17.1 69,900 6.2 4 3

PAI-1 0.7 2,922 0.5 5 2

Resistin 125.9 257,870 32.0 4 4

* Due to the different dilution scheme, adiponectin was assayed as a single assay.



The Bio-Plex Pro Mouse Diabetes Assays offer reliable performance and a convenient format for studying mouse diabetes and obesity markers. These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (<3 hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 μl of sample.


Bulletin 6335

35

Bio-Plex Pro Rat Cytokine and TGF-b Assays

Bio-Plex Pro Rat Cytokine Singleplex Sets


IL-12 (p40)** 171L1015M 76

MIP-2*** 171L1022M 27

** IL-12 (p40) cannot be multiplexed with IL-12 (p70.)*** MIP-2 cannot be multiplexed with GRO/KC.

Bio-Plex Pro Rat Cytokine 24-Plex Panel (171K1001M)


EPO 171L1002M 14

G-CSF 171L1003M 54

GM-CSF 171L1004M 37

Gro/KC 171L1005M 57

IFN-g 171L1006M 34

IL-1a 171L1007M 21

IL-1b 171L1008M 28

IL-2 171L1009M 22

IL-4 171L1010M 33

IL-5 171L1011M 52

IL-6 171L1012M 56

IL-7 171L1013M 38

IL-10 171L1014M 19

IL-12 (p70) 171L1016M 78

IL-13 171L1017M 15

IL-17A 171L1018M 72

IL-18* 20

M-CSF 171L1020M 26

MCP-1 / CCL2 171L1027M 51

MIP-1a / CCL3 171L1021M 77

MIP-3a / CCL20 171L1023M 36

RANTES 171L1024M 55

TNF-a 171L1025M 43

VEGF 171L1026M 47

* IL-18 is not available as a singleplex assay but only as part of an assay panel.

Bio-Plex Pro TGF-b 3-Plex Panel (171W4001M)


TGF-b1 171V4001M 13

TGF-b2 171V4002M 72

TGF-b3 171V4003M 66

Bio-Plex Pro Rat Cytokine 12-Plex Th1/Th2 Panel (171K1002M)


GM-CSF 171L1004M 37

IFN-g 171L1006M 34

IL-1a 171L1007M 21

IL-1b 171L1008M 28

IL-2 171L1009M 22

IL-4 171L1010M 33

IL-5 171L1011M 52

IL-6 171L1012M 56

IL-10 171L1014M 19

IL-12 (p70) 171L1016M 78

IL-13 171L1017M 15

TNF-a 171L1025M 43

Rat Assays

Performance CharacteristicsRat Cytokine

Assay Working Ranges, Assay Sensitivity, pg/ml pg/ml Assay Precision


Singleplex Assay

IL-12p40 2 2,015 0.4 3 4

MIP-2 0.3 1,085 0.2 4 5

23-Plex Assay

EPO 32 32,258 8 4 1

G-CSF 1 2,911 0.2 2 1

GM-CSF 6 31,519 0.6 4 1

Gro/KC 2 1,521 0.6 4 2

IFN-g 4 23,955 1 5 3

IL-1a 2 24,649 1 3 3

IL-1b 6 31,666 2 4 2

IL-2 8 7,981 3 4 3

IL-4 1 16,209 1 6 3

IL-5 6 2,007 6 4 0.4

IL-6 13 30,766 10 4 3

IL-7 2 32,472 0.4 4 3

IL-10 4 34,385 5 4 6

IL-12 (p70) 4 15,481 0.7 4 3

IL-13 2 15,751 0.9 4 2

IL-17A 1 3,079 0.1 3 2

IL-18 4 31,652 4 4 5

MCP-1 / CCL2 12 48,000 4 4 4

M-CSF 2 23,264 0.4 2 3

MIP-1a / CCL3 25 4,325 12 4 7

MIP-3a / CCL20 1 3,032 0.7 4 2

RANTES 8 7,950 3 5 2

TNF-a 16 8,207 3 5 7

VEGF 0 3,963 0.3 3 5

bio-rad.com/CCGFassaysBio-Rad bulletins 6100 and 6054

Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Magnetic Bead–Based Assays detect and measure levels of analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth to infections to Parkinson’s disease.



36

Bio-Plex Pro Rat Cell Signaling Assaysbio-rad.com/bio-plexprodiabetes



Lysate Controls Lysate Controls for Total Protein for Total Protein Phosphoprotein Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat.

Akt (Ser473) 171V50001M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002

Akt (Thr308) 171V50002M EGF-treated HEK-293 171YZ0001 171V60001M Untreated HeLa 171YZT002

ATF-2 (Thr71) 171V50024M UV-treated HEK-293 171YZ0009 — — —

BAD (Ser136) 171V50025M PDGF-treated NIH3T3 171YZ0007 — — —

c-Jun (Ser63) 171V50003M UV-treated HEK-293 171YZ0009 171V60002M Untreated HEK-293 171YZT001

CREB (Ser133) 171V50028M UV-treated HEK-293 171YZ0009 171V60013M Untreated HEK-293 171YZT001

GSK-3a/b (Ser21/Ser9) 171V50007M EGF-treated HEK-293 171YZ0001 171V60004M Untreated HeLa 171YZT002

HER-2 (Tyr1248) 171V50008M EGF-treated SK-Br3 171YZ0003 171V60005M EGF-treated SK-Br3 171YZ0003

IR-b (Tyr1146) 171V50031M TNF-a-treated HeLa 171YZ0008 — — —

IRS-1 (Ser636/Ser639) 171V50030M PDGF-treated NIH3T3 171YZ0007 — — —

Lyn (Tyr507) 171V50032M H2O2-treated Ramos 171YZ0011 — — —

MEK1 (Ser217/Ser221) 171V50012M EGF-treated HEK-293 171YZ0001 171V0008M Untreated HeLa 171YZT002

mTOR (Ser2448) 171V50033M PDGF-treated NIH3T3 171YZ0007 171V60015M Untreated HeLa 171YZT002

NF-kb p65 (Ser536) 171V50013M TNF-a-treated HeLa 171YZ0008 — — —

p38 MAPK (Thr180/Tyr182) 171V50014M UV-treated HEK-293 171YZ0009 171V60009M Untreated HeLa 171YZT002

p70 S6 Kinase (Thr389) 171V50015M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002

p70 S6 Kinase (Thr421/Ser424) 171V50016M b-NGF-treated PC12 171YZ0006 171V60010M Untreated HeLa 171YZT002

p90 RSK (Ser380) 171V50035M EGF-treated SK-Br3 171YZ0003 — — —


Bulletin 6335

37

Bio-Plex Pro Rat Cell Signaling Assays bio-rad.com/cellsignalingassaysBio-Rad bulletin 6285

Bio-Plex Pro Rat Cell Signaling Phosphoprotein Assays

Lysate Controls Lysate Controls Total Protein for Total Protein Phosphoprotein for Singleplex Cat. Phosphoprotein Assays Lysate Cat. Singlplex Cat. Assays Lysate Cat.

PDGFR-b (Tyr751) 171V50018M PDGF-treated NIH3T3 171YZ0007 — — —

Pl3K p85 (Tyr458) 171V50036M H2O2-treated Ramos 171YZ0011 — — —

PTEN (Ser380) 171V50037M PDGF-treated NIH3T3 171YZ0007 171V60016M Untreated HeLa 171YZT002

S6 ribosomal protein (Ser235/Ser236) 171V50038M EGF-treated SK-Br3 171YZ0003 — — —

Smad2 (Ser465/Ser467) 171V50019M TNF-a-treated HeLa 171YZ0008 171V60011M Untreated HeLa 171YZT002

Src (Tyr416) 171V50039M Src-transfected NIH3T3 171YZ0013 171V60017M Src-transfected NIH3T3 171YZ0013

Stat3 (Ser727) 171V50021M IFN-a-treated HeLa 171YZ0004 — — —

Stat3 (Tyr705) 171V50022M IFN-a-treated HeLa 171YZ0004 — — —

Syk (Tyr352) 171V50040M H2O2-treated Ramos 171YZ0011 — — —

Bio-Plex Pro Rat Cell Signaling Akt Panel 8-Plex (LQ-00006JK0K0RR)

Analyte












* See pages 21–22 for additional performance characteristics.



38

Bio-Plex Pro Rat Diabetes Assays

Rat Diabetes



Ghrelin 1 16,152 0.3 4 4

GLP-1 4 6,062 3 8 6

Glucagon 6 4,443 6 3 4

Leptin 24 130,465 5 4 3

PAI-1 49 66,888 27 5 3



Bio-Plex Pro Rat Diabetes Singleplex Sets


Ghrelin 171L7001M 64

GLP-1 171L7003M 62

Glucagon 171L7004M 63

Leptin 171L7006M 65

PAI-1 171L7007M 61

Bio-Plex Pro Rat Diabetes Assays offer reliable performance and a convenient format for studying rat diabetes and obesity markers. These assays are magnetic bead–based assays, available as either premixed panels or singleplex sets, that rapidly (<3 hr) detect multiple diabetes and obesity biomarkers in a single experiment, using as little as 12.5 μl of sample.


Bulletin 6335

39

Bio-Plex Pro Rat Kidney Toxicity Assays

Bio-Plex Pro RBM Rat Kidney Toxicity Albumin Kit (171KTR3CK)

Analyte Bead Region

Albumin 30

Bio-Plex Pro RBM Rat Kidney Toxicity Panel 1 (171KTR1CK)

Analyte Bead Region

Clusterin 12

IL-18 21

KIM-1 20

MCP-1 / CCL2 15

Osteopontin 52

Bio-Plex Pro RBM Rat Kidney Toxicity Panel 2 (171KTR2CK)

Analyte Bead Region

B2M 22

Calbindin 62

Cystatin C 44

NGAL 46



Rat Kidney Toxicity Panel 1

Clusterin ApoJ 0.99 840 0.43 3 12

IL-18 0.36 35 0.17 4 5

KIM-1 TIM-1 0.042 50 0.023 4 7

MCP-1 / CCL2 0.15 35 0.045 2 6

Osteopontin OPN 0.0039 3.2 0.0015 4 5

Rat Kidney Toxicity Panel 2

B2M 8.3 2,760 4.1 10 18

Calbindin 0.7 535 0.61 9 14

Cystatin C 0.062 42 0.026 2 6

NGAL Lipocalin-2 0.63 480 0.22 5 10

Rat Kidney Toxicity Albumin Kit

Albumin 0.043 35 0.014 5 9


The Bio-Plex Pro RBM Rat Kidney Toxicity Assays, developed in partnership with Myriad RBM, comprise a highly relevant set of biomarkers for early detection and characterization of kidney injury. Myriad RBM’s close collaboration with the Predictive Safety Testing Consortium (PSTC), FDA, and European Medicines Evaluation Agency (EMEA) was instrumental in the selection of the markers found in these panels. The assays are built on magnetic beads to enable robust quantification of multiple proteins in rat urine samples, providing valuable information throughout drug development — from lead optimization to preclinical and clinical protocol decision making.



Bio-Plex Pro Canine Kidney Toxicity AssaysCanine Assays


40

The Bio-Plex Pro RBM Canine Kidney Toxicity Assays, developed in partnership with Myriad RBM, comprise a highly relevant set of biomarkers for early detection and characterization of kidney injury. Myriad RBM’s close collaboration with the Predictive Safety Testing Consortium (PSTC), FDA, and European Medicines Evaluation Agency (EMEA) was instrumental in the selection of the markers found in these panels. The assays are built on magnetic beads to enable robust quantification of multiple proteins in canine urine samples, providing valuable information throughout drug development — from lead optimization to preclinical and clinical protocol decision making.

Bio-Plex Pro RBM Canine Kidney Toxicity Panel 1 (171QTR1CK)

Analyte Bead Region

Clusterin 12

KIM-1 53

MCP-1 / CCL2 15

NGAL 46

Bio-Plex Pro RBM Canine Kidney Toxicity Albumin Kit (171QTR2CK)

Analyte Bead Region

Albumin 30



Canine Kidney Toxicity Panel 1

Clusterin ApoJ 0.69 800 0.65 4 6 KIM-1 TIM-1 0.012 10 0.0046 4 7 MCP-1 / CCL2 0.013 4.8 0.0043 5 12 NGAL Lipocalin-2 0.010 9.0 0.0042 5 7

Canine Kidney Toxicity Albumin Kit

Albumin 0.0052 4.8 0.0022 8 11


Bulletin 6335

Bio-Plex Comprehensive Analyte Tables*

continues

41

6Ckine / CCL21 Chemokine 40-plex (p 14)

AAT Metabolic Panel 3 (p 25)

Adiponectin Diabetes (p 24) Diabetes (p 36) Diabetes (p 24)

Adipsin Diabetes 2-plex (p 24) Diabetes (p 24)

AGP-1 Metabolic Panel 3 (p 25)

Albumin Kidney Toxicity 2 6-plex (p 27) Kidney Toxicity (p 39) Kidney Toxicity (p 40)

Angiopoietin-2 Cancer 2 18-plex (p 18)

ANGPTL3 Metabolic Panel 4 (p 25)

APRIL / TNFSF13 Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)†

Bad Apoptosis 2 (p 23)

BAFF / TNFSF13B Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)†

Bak Apoptosis 1 (p 23)

Bax Apoptosis 1 (p 23)

Bax / Bcl-2 dimer Apoptosis 2 (p 23)

BCA-1 / CXCL13 Chemokine 40-plex (p 14)

Bcl-xL Apoptosis 2 (p 23)

Bcl-xL / Bak dimer Apoptosis 3 (p 23)

Bim Apoptsis 2 (p 23)

B2M Kidney Toxicity 2 6-plex (p 27) Kidney Toxicity 2 4-plex (p 39)

Calbindin Kidney Toxicity 1 6-plex (p 27) Kidney Toxicity 2 4-plex (p 39)

Caspase-3, active Apoptosis 3 (p 23)

sCD30 / TNFRSF8 Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8)

sCD163 Inflammation 1 37-plex (p 8)† † Inflammation 1 24-plex (p 8)† †

sCD40L Cancer 2 18-plex (p 18) Group III Cytokine Th17 (p 31) Th17 (p 13)

Chemerin Metabolic Panel 4 (p 25)

Chitinase 3-like 1 Inflammation 1 37-plex (p 8)† † Inflammation 1 24-plex (p 8)† †

Clusterin Kidney Toxicity 1 6-plex (p 27) Kidney Toxicity 1 5-plex (p 39) Kidney Toxicity 1 4-plex (p 40)

.

Target Human (p 6–29) Mouse (p 30–34) Rat (p 35–39) Non-Human Primate (p 24) Canine (p 40)

* For cell signaling analytes, see page 19.† Of the top Luminex partners, Bio-Rad is the exclusive provider of this assay.† † Assay not available from Vendor M.


continues

42

Cortisol Metabolic Panel 1 (p 25)

C-Peptide Diabetes 10-plex (p 24) Diabetes 10-plex (p 24) Metabolic Panel 1 (p 25)

CRP Acute Phase 4-plex (p 29)

CTACK / CCL27 Group II Cytokine 21-plex (p 7) Chemokine 40-plex (p 14)

Cystatin C Kidney Toxicity 2 6-plex (p 27) Kidney Toxicity 2 4-plex (p 39)

DPP4 Metabolic Panel 4 (p 25)

EGF Cancer 2 18-plex (p 18)

ENA-78 / CXCL5 Chemokine 40-plex (p 14)

sEGFR Cancer 1 16-plex (p 17)

Endoglin Cancer 2 18-plex (p 18)

Eotaxin / CCL11 Group l Cytokine 27-plex (p 6) Group I Cytokine 23-plex (p 30) Chemokine 40-plex (p 14)

Eotaxin-2 / CCL24 Chemokine 40-plex (p 14)

Eotaxin-3 / CCL26 Chemokine 40-plex (p 14)

EPO Cytokine 24-plex (p 35)

sFASL Cancer 2 18-plex (p 18)

Ferritin Acute Phase 5-plex (p 29)

FGF-21 Metabolic Panel 2 (p 25)

FGF-23 Metabolic Panel 2 (p 25)

FGF-basic Group I Cytokine 27-plex (p 6) Group II Cytokine 9-plex (p 31) Cancer 1 16-plex (p 17)

Fibrinogen Acute Phase 5-plex (p 29)

Follistatin Cancer 1 16-plex (p 17)

Fractalkine / Chemokine 40-plex (p 14) CX3CL1

FSH Hormone Panel 1 (p 25)

Galectin-3 Metabolic Panel 2 (p 25)

GCP-2 / CXCL6 Chemokine 40-plex (p 14)

G-CSF Group I Cytokine Group I Cytokine Cytokine 24-plex (p 35) 17-plex/27-plex (p 6) 23-plex (p 30) Cancer 1 16-plex (p 17)

GH Hormone Panel 1 (p 25)

.


Bulletin 6335

43

Ghrelin Diabetes 10-plex (p 24) Diabetes 8-plex (p 34) Diabetes (p 38) Diabetes 10-plex (p 24)

GIP Diabetes 10-plex (p 24) Diabetes 8-plex (p 34) Diabetes 10-plex (p 24)

GLP-1 Diabetes 10-plex (p 24) Diabetes 8-plex (p 34) Diabetes (p 38) Diabetes 10-plex (p 24)

Glucagon Diabetes 10-plex (p 24) Diabetes 8-plex (p 34) Diabetes (p 38) Diabetes 10-plex (p 24)

GM-CSF Group I Cytokine 8-plex/ Group I Cytokine Cytokine 24-plex/ 17-plex/27-plex (p 6) 8-plex/23-plex/[Th1/Th2] (p 30) [Th1/Th2] (p 35) Chemokine 40-plex (p 14)

gp130 / sIL-6Rb Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8)

Gro-a / KC / CXCL1 Group II Cytokine 21-plex (p 7) Chemokine 40-plex (p 14)

Gro-b / CXCL2 Chemokine 40-plex (p 14)

GST-π Kidney Toxicity 1 6-plex (p 27)

Haptoglobin Acute Phase 4-plex (p 29)

HB-EGF Cancer 2 18-plex (p 18)

Hemopexin Metabolic Panel 3 (p 25)

sHER-2 / neu Cancer 1 16-plex (p 17)

HGF Cancer 1 16-plex (p 17) Group II Cytokine 21-plex (p 7)

I-309 / CCL1 Chemokine 40-plex (p 14)

ICAM-1 Group II Cytokine singleplex (p 7) Group III singleplex (p 31)

IFN-a2 Group II Cytokine 21-plex (p 7) Inflammation 1 37-plex (p 8)† † †

IFN-b Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)†

IFN-g Group l Cytokine 8-plex/ Group I Cytokine 8-plex/ Cytokine 24-plex/[Th1/Th2] (p 35) 17-plex/27-plex/[Th1/Th2] (p 6) 23-plex/[Th1/Th2] (p 30) Inflammation 1 37-plex (p 8) Th17 (p 31) Chemokine 40-plex (p 14)

IgA Isotyping 6-plex (p 28)

IgE Isotyping singleplex (p 28)

IGF-1 IGF Panel (p 25)

IGF-2 IGF Panel (p 25)

IGFBP-1 Cancer 2 18-plex (p 18) IGFBP Panel (p 25)

.


† Of the top Luminex partners, this assay is offered exclusively by Bio-Rad.† † † Assay not available from Vendor R.

continues


44

IGFBP-2 IGFBP Panel (p 25)






IgG1 Isotyping 6-plex (p 28)




IgG-total Isotyping singleplex (p 28)

IgM Isotyping 6-plex (p 28)

IL-1a Group ll Cytokine 21-plex (p 7) Group I Cytokine 23-plex (p 30) Cytokine Th1/Th2 (p 35)

IL-1b Group I Cytokine Group I Cytokine Cytokine Th1/Th2 (p 35) 17-plex/27-plex (p 6) 8-plex/23-plex (p 30) Th17 Cytokine 15-plex (p 13) Chemokine 40-plex (p 14)

IL-1Ra Group I Cytokine 27-plex (p 6)

IL-2 Group l Cytokine 8-plex/ Group I Cytokine 8-plex/ Cytokine Th1/Th2 (p 35) 17-plex/27-plex/[Th1/Th2] (p 6) 23-plex/[Th1/Th2] (p 30) Inflammation 1 37-plex (p 8) Inflammation 1 Treg (p 8) Chemokine 40-plex (p 14)

IL-2Ra Group II Cytokine 21-plex (p 7)

IL-3 Group II Cytokine 21-plex (p 7) Group I Cytokine 23-plex (p 30)

IL-4 Group l Cytokine Group I Cytokine Cytokine Th1/Th2 (p 35) 8-plex/17-plex/27-plex/ 8-plex/23-plex/[Th1/Th2] (p 30) [Th1/Th2] (p 6) Th17 Cytokine 15-plex (p 13) Chemokine 40-plex (p 14)

IL-5 Group l Cytokine Group I Cytokine Cytokine Th1/Th2 (p 35) 17-plex/27-plex/[Th1/Th2] (p 6) 8-plex/23-plex/[Th1/Th2] (p 30)

IL-6 Group l Cytokine 8-plex/ Group I Cytokine Cytokine 24-plex/[Th1/Th2] (p 35) 17-plex/27-plex (p 6) 23-plex/[Th1/Th2]/Th17 (p 30) Chemokine 40-plex (p 14) Th17 6-plex (p 31) Cancer 2 18-plex (p 18)

.


continues

Bulletin 6335

45

.


sIL-6Ra Inflammation 1 37-plex (p 8) Cancer 1 16-plex (p 17)

IL-7 Group l Cytokine Cytokine 24-plex (p 35) 17-plex/27-plex (p 6)

IL-8 / CXCL8 / CXCL8 Group l Cytokine 8-plex/ 17-plex/27-plex (p 6) Inflammation 1 37-plex (p 8) Chemokine 40-plex (p 14)) Cancer 2 18-plex (p 18)

IL-9 Group I Cytokine 27-plex (p 6) Group I Cytokine 23-plex (p 30)

IL-10 Group l Cytokine 8-plex/ Group I Cytokine Cytokine 24-plex/[Th1/Th2] (p 31) 17-plex/27-plex/[Th1/Th2] (p 6) 8-plex/23-plex/ Inflammation 1 37-plex (p 8) [Th1/Th2]/Th17 (p 30) Inflammation 1 Treg (p 8) Th17 6-plex (p 31) Th17 (p 13) Chemokine 40-plex (p 14)

IL-11 Inflammation 1 37-plex (p 8)† † † Inflammation 1 24-plex (p 8)† † †

IL-12 (p40) Group II Cytokine 21-plex (p 7) Group I Cytokine 23-plex (p 30) Cytokine 24-plex (p 35) Inflammation 1 37-plex (p 8)† † † Inflammation 1 Treg (p 8)† † †

IL-12 (p70) Group l Cytokine 17-plex/ Group I Cytokine Cytokine 24-plex/[Th1/Th2] (p 35) 27-plex/[Th1/Th2] (p 6) 23-plex/[Th1/Th2] (p 30) Inflammation 1 37-plex (p 8) Inflammation 1 Treg (p 8)

IL-13 Group l Cytokine 17-plex/ Group I Cytokine 23-plex/Th2 (p 30) Cytokine 24-plex/[Th1/Th2] (p 35) 27-plex/[Th1/Th2] (p 6)

IL-15 Group I Cytokine 27-plex (p 6) Group II Cytokine 9-plex (p 31)

IL-16 Group II Cytokine 21-plex (p 7) Chemokine 40-plex (p 14)

IL-17A Group I Cytokine Cytokine 23-plex (p 30) Cytokine 24-plex (p 35) 17-plex/27-plex (p 6) Th17 6-plex (p 31) Th17 Cytokine 15-plex (p 13)

IL-17A/F Th17 Cytokine singleplex (p 13)

IL-17E / IL-25 Th17 Cytokine 15-plex (p 13) Cytokine Group II singleplex (p 31)

IL-17F Th17 Cytokine 15-plex (p 13) Group III Cytokine Th17 8-plex (p 31)

IL-18 Group II Cytokine 21-plex (p 7) Group II Cytokine 9-plex (p 31) Cytokine 24-plex (p 35) Cancer 2 18-plex (p 18) Kidney Toxicity 1 5-plex (p 39) Kidney Toxicity 1 6-plex (p 27)

continues† † † Assay not available from Vendor R.


46

.


IL-19 Inflammation 1 37-plex (p 8)† † Inflammation 1 24-plex (p 8)† † Inflammation 1 Treg (p 8)† †

IL-20 Inflammation 1 37-plex (p 8)† †† Inflammation 1 24-plex (p 8)††† Inflammation 1 Treg (p 8)†††

IL-21 Th17 Cytokine 15-plex (p 13) Cytokine III Th17 (p 31)

IL-22 Inflammation 1 37-plex (p 8) Inflammation 1 Treg (p 8) Th17 Cytokine 15-plex (p 13)

IL-23 (p19) Th17 Cytokine 15-plex (p 13) Cytokine III Th17 (p 31)

IL-25 / IL-17E Th17 Cytokine 15-plex (p 13) Cytokine Group II singleplex (p 31)

IL-26 Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)† Inflammation 1 Treg (p 8)†

IL-27 (p28) Inflammation 1 37-plex (p 8) Group III Cytokine (p 31) Inflammation 1 24-plex (p 8) Inflammation 1 Treg (p 8)

IL-28A / IFN-l2 Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8) Inflammation 1 Treg (p 8)

IL-29 / IFN-l1 Inflammation 1 37-plex (p 8)† †† Inflammation 1 24-plex (p 8)† †† Inflammation 1 Treg (p 8)† ††

IL-32 Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)†

IL-33 Th17 Cytokine 15-plex (p 13) Cytokine III Th17 (p 31)

IL-34 Inflammation 1 37-plex (p 8)† † Inflammation 1 24-plex (p 8)† †

IL-35 Inflammation 1 37-plex (p 8)† Inflammation 1 24-plex (p 8)† Inflammation 1 Treg (p 8)†

Insulin Diabetes 10-plex (p 24) Diabetes 8-plex (p 34) Diabetes 10-plex (p 24)

IP-10 / CXCL10 Group I Cytokine 27-plex (p 6) Chemokine 40-plex (p 14)

I-TAC / CXCL11 Chemokine 40-plex (p 14)

KC / Gro-a / CXCL1 Group I Cytokine 23-plex (p 30)

continues† Of the top Luminex partners, this assay is offered exclusively by Bio-Rad.† † Assay not available from Vendor M.† † † Assay not available from Vendor R.

Bulletin 6335

47

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KIM-1 Kidney Toxicity 1 6-plex (p 27) Kidney Toxicity 1 5-plex (p 39) Kidney Toxicity 1 4-plex (p 40)

Lamin B, intact Apoptosis 1 (p 23) and 45 kD

Leptin Cancer 1 16-plex (p 17) Diabetes 8-plex (p 34) Diabetes (p 38) Diabetes 10-plex (p 24) Diabetes 10-plex (p 24)

sLeptin R Metabolic Panel 2 (p 25)

LH Hormone Panel 1 (p 25)

LIF Group II Cytokine 21-plex (p 7) Group II Cytokine 9-plex (p 31)

LIGHT / TNFSF14 Inflammation 1 37-plex (p 8)† † † Inflammation 1 24-plex (p 8)† † †

a-2-macroglobin Acute Phase 5 + 4-plex/4-plex (p 29)

Mcl-1 Apoptosis 2 (p 23)

Mcl-1 / Bak dimer Apoptosis 3 (p 23)

MCP-1 / CCL2 Group I Cytokine Group I Cytokine 23-plex (p 30) Cytokine 24-plex (p 35) Kidney Toxicity 1 4-plex (p 40) 17-plex/27-plex (p 6) Kidney Toxicity 1 5-plex (p 39) Chemokine 40-plex (p 14) Kidney Toxicity 1 6-plex (p 27)

MCP-2 / CCL8 Chemokine 40-plex (p 14)

MCP-3 / CCL7 Group II Cytokine 21-plex (p 7) Chemokine 40-plex (p 14)

MCP-4 / CCL13 Chemokine 40-plex (p 14)

M-CSF Group II Cytokine 21-plex (p 7) Group II Cytokine 9-plex (p 31) Cytokine 24-plex (p 35)

MDC / CCL22 Chemokine 40-plex (p 14)

MIF Group II Cytokine 21-plex (p 7) Chemokine 40-plex (p 14)

MIG / CXCL9 Group II Cytokine 21-plex (p 7) Group II Cytokine 9-plex (p 31) Chemokine 40-plex (p 14)

MIP-1a / CCL3 Group I Cytokine 27-plex (p 6) Group I Cytokine 23-plex (p 30) Cytokine 24-plex (p 35) Chemokine 40-plex (p 14)

MIP-1d / CCL15 Chemokine 40-plex (p 14)

MIP-1b Group I Cytokine Group I Cytokine 23-plex (p 30) 17-plex/27-plex (p 6)

MIP-2 Group II Cytokine 9-plex (p 31) Cytokine (p 37)

MIP-3a / CCL20 Chemokine 40-plex (p 14) Group III Cytokine Cytokine 24-plex (p 35) Th17 8-plex (p 31)

continues† † † Assay not available from Vendor R.


48

continues

.


MIP-3b / CCL19 Chemokine 40-plex (p 14)

MMP-1 Inflammation 1 37-plex (p 8) MMP 9-plex (p 12)



MMP-7 MMP 9-plex (p 12)






MPIF-1 / CCL23 Chemokine 40-plex (p 14)

NGAL Kidney Toxicity 2 6-plex (p 27) Kidney Toxicity 2 4-plex (p 39) Kidney Toxicity 1 4-plex (p 40)

b-NGF Group II Cytokine 21-plex (p 7)

Omentin-1 Metabolic Panel 2 (p 25)

Osteocalcin Inflammation 1 37-plex (p 8)† † † Inflammation 1 24-plex (p 8)† † †

Osteopontin Inflammation 1 37-plex (p 8) Kidney Toxicity 1 5-plex (p 39) Cancer 1 16-plex (p 17) Kidney Toxicity 2 6-plex (p 27)

PAI-1 Cancer 2 18-plex (p 18) Diabetes 8-plex (p 34) Diabetes singleplex (p 38) Diabetes 10-plex (p 24) Diabetes 10-plex (p 24)

Pancreatic polypeptide Metabolic Panel 1 (p 25)

PDGF-AB / BB Group I Cytokine 27-plex (p 6) Group II Cytokine 9-plex (p 31) Cancer 1 16-plex (p 17)

PECAM-1 Cancer 1 16-plex (p 17)

Pentraxin-3 Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8) Metabolic Panel 2 (p 25)

Peptide YY Metabolic Panel 1 (p 25)

PLGF Cancer 2 18-plex (p 18)

PON-1 Metabolic Panel 2 (p 25)

† † † Assay not available from Vendor R.

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49

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continues

Procalcitonin Acute Phase 5 + 4-plex/5-plex (p 29)

Proinsulin Metabolic Panel 1 (p 25)

Prolactin Cancer 1 16-plex (p 17) Hormone Panel 1 (p 25)

Protein S Metabolic Panel 4 (p 25)

RANTES Group I Cytokine 27-plex (p 6) Group I Cytokine 23-plex (p 30)

RBP-4 Metabolic Panel 3 (p 25)

Resistin Diabetes 10-plex (p 24) Diabetes 8-plex (p 30) Diabetes 10-plex (p 24)

SAA Acute Phase 5 + 4-plex/5-plex (p 29)

SAP Acute Phase 5 + 4-plex/4-plex (p 29)

SCF Group II Cytokine 21-plex (p 7) Cancer 1 16-plex (p 17)

SCGF-b Group II Cytokine 21-plex (p 7)

SCYB16 / CXCL16 Chemokine 40-plex (p 14)

SDF-1a+b / Group II Cytokine 21-plex (p 7) CXCL12 Chemokine 40-plex (p 14)

SEPP Metabolic Panel 4 (p 25)

Smac Apoptosis 1 (p 23)

Survivin Apoptosis 3 (p 23)

TARC / CCL17 Chemokine 40-plex (p 14)

TECK / CCL25 Chemokine 40-plex (p 14)

TFF3 Kidney Toxicity 2 6-plex (p 27)

TGF-a Cancer 2 18-plex (p 18)

TGF-b1 TGF-b (p 16) TGF-b (p 31) TGF-b (p 35)



sTIE-2 Cancer 1 16-plex (p 17)

TIMP-1 TIMP 4-plex (p 12)





50

.


† † † Assay not available from Vendor R.

TNF-a Group I Cytokine 8-plex/ Group I Cytokine 8-plex/ Cytokine Th1/Th2 (p 35) 17-plex/27-plex/[Th1/Th2] (p 6) 23-plex/[Th1/Th17] (p 30) Th17 (p 13) Th17 6-plex (p 31) Chemokine 40-plex (p 14) Cancer 2 18-plex (p 18)

TNF-b Group II Cytokine 21-plex (p 7)

sTNF-R1 Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8)

sTNF-R2 Inflammation 1 37-plex (p 8) Inflammation 1 24-plex (p 8)

tPA Acute Phase 5 + 4-plex/5-plex (p 29)

TRAIL Group II Cytokine 21-plex (p 7)

Transferrin Metabolic Panel 3 (p 25)

Transthyretin Metabolic Panel 3 (p 25)

TSH Hormone Panel 1 (p 25)

TSLP Inflammation 1 37-plex (p 8)† † † Inflammation 1 24-plex (p 8)† † †

TWEAK / TNFSF12 Inflammation 1 37-plex (p 8)† † † Inflammation 1 24-plex (p 8)† † †

uPA Cancer 2 18-plex (p 18)

Vaspin Metabolic Panel 2 (p 25)

VCAM-1 Group II Cytokine singleplex (p 7)

VDBP Metabolic Panel 3 (p 25)

VEGF Group I Cytokine singleplex (p 6) Group II Cytokine 9-plex (p 31) Cytokine 24-plex (p 35)

VEGF-A Cancer 2 18-plex (p 18)

VEGF-C Cancer 2 18-plex (p 18)

VEGF-D Cancer 2 18-plex (p 18)

sVEGFR-1 Cancer 1 16-plex (p 17)

sVEGFR-2 Cancer 1 16-plex (p 17)

Visfatin Diabetes 10-plex (p 24) Diabetes 10-plex (p 24)

Notes

Bulletin 6335 Ver E US/EG 16-0434 0516 Sig 1215

Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 177 Belgium 32 (0)3 710 53 00 Brazil 55 11 3065 7550 Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 45 44 52 10 00 Finland 358 09 804 22 00 France 33 01 47 95 69 65 Germany 49 89 31 884 0 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300 Israel 972 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 31 (0)318 540 666 New Zealand 64 9 415 2280 Norway 47 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 (0) 861 246 723 Spain 34 91 590 5200 Sweden 46 08 555 12700 Switzerland 41 026 674 55 05 Taiwan 886 2 2578 7189 Thailand 66 662 651 8311 United Arab Emirates 971 4 8187300 United Kingdom 44 020 8328 2000

Bio-Rad Laboratories, Inc.

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The Bio-Plex Suspension Array System includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

CST antibodies developed and validated for Bio-Plex Phosphoprotein and Total Target Assays.

FLEXMAP 3D, Luminex, MAGPIX, MagPlex, and xMAP are trademarks of Luminex Corporation.

RBM Assays are manufactured by Myriad RBM. Myriad RBM™ is a trademark of Myriad Genetics, Inc.

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http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6335.pdf

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