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SRM UNIVERSITY
SCHOOL OF BIOENGINEERING
DEPARTMENT OF BIOTECHNOLOGY
BT0403-BIORECTOR DESIGN
VII SEMESTER-B.Tech Biotechnology
PURPOSE:
The course imparts advanced knowledge on bioreactor design for efficient
utilization of the principles in bioprocess technology.
INSTRUCTIONAL OBJECTIVES:
1. To make the students to understand the basic concepts of bioreactor design
2. To make them to understand the instrumentation and control of bioreactor
3. To understand the strategies of fermentation control
4. To understand the concepts of modeling and simulation
5. To understand the concepts of plant and animal cell bioreactors
S.
NO
DA
Y
UNI
T TOPICS LEARNING OUT COMES
HOUR
S
CUM.
HOURS
STUDENTS CAN ABLE TO
UNDERSTAND
1 I BIOREACTOR DESIGN
1 Types of bioreactor Different types of reactor 1 1
2 Heat transfer in Bioreactor Heat transfer in reactor 1 2
6 Scale –up in Bioreactor Scale up of reactors 4 6
7 Airlift reactor 1 7
8 Introduction 1 8
9 Design of ALR Design of ALR 1 9
11 Construction Construction 2 10
12 Hydrodynamic studies Hydrodynamic studies 1 11
13 Three phase flow Three phase flow 1 12
14 Mixing Mixing 1 13
14 Oxygen transfer Oxygen transfer 1 14
II BATCH AND
CONTINUOUS GROWTH
16 Growth,Measurement of
Microbial
growth
Growth of microbes 2 16
Growth of microbial
growth(indirect)
Growth of microbes
17 Kinetics of Microbial growth
in batch culture
Kinetics 1 17
19 Continuous culture Continuous system 2 19
23 Problems 4 23
III MIXING ,MASS
TRANSFER AND
INSTRUMENTATION
CONTROL OF
BIOREACTOR
24 Introduction 1 24
25 Mass Transfer Mass Transfer in microbial and
reactor
2 25
27 Problem 2 27
29 Theory of Mixing
Parameters using sensor
1 28
31 Rheological Properties 2 30
Bioreactor sensor
Characteristics
32 Temperature measurement
control
1 31
33 Principles of dissolved
oxygen measurement and
control
1 32
34 Principles of pH/Redox
measurement
2 34
Deduction and Preventation
of foam
Deduction and preventation
of foam
35 Determination of biomass
and application of biosensor
1 35
IV BIORECTOR OFF- GAS
ANALYSIS
36 INTRODUCTION 1 36
38 Generalized gas mass
balance equations
Mass balance in gas transfer 2 38
39 Steady state balance Steady state balance 1 39
41 Derived quantities based on
combined gas analysis and
gas mass balancing
techniques
Derived quantities and gas analysis 2 41
42 Gas analysers Various gas analysers 1 42
V MODELING OF PLANT
AND ANIMAL CELL
BIOREACTOR
43 Modelling Modeling
Plant and animal cell Bioreactor
design
1 43
45 Digital
simulation,Formulation and
solution of problems
2 45
47 ISIM 2 47
50 Plant cell and Animal cell 3 50
TEXTBOOK:
“Bioreactors in Biotechnology” Ellis Horwood series,1991, A.H.SCRAGG
Course Co-ordinator
HOD
Mr.M.Venkatesh Prabhu B.E (Chem).,M.Tech.,D.I.S.,(P.hD)
SRM UNIVERSITY
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING
Subject code: BT 0407 Semester: VII
Subject: Bioseparation Technology Year: 2011 -2012
LECTURE SCHEDULE
S.No Hours to
be taken Topic / Content Books Learning outcomes
UNIT I
INTRODUCTION TO BIOSEPARATION PROCESS
1. 1 Role and importance of Bioseparation process 1,2 To understand the role
and importance of
downstream processing
in biotechnological
processes.
To understand nature
of the fermentation
broth, size, shape and
the rheological
properties.
To learn the economics
and various cost
cutting strategies of
product purification.
2. 1 Problems and requirements of bioproduct
purification 2,3
3. 1 Cost- cutting strategies 2,3
4. 1 Properties of biomolecules 3
5. 1 Characteristics of fermentation broth 4
6. 1 Process of Classification of Bioproducts 3
7. 1 Analysis of Biological activity 2
8. 1 Analysis of purity of biomolecules 2
9. 1 Process economics-Capital and operating cost
analysis. 2
UNIT II
CELL DISRUPTION AND SEDIMENTATION
10. 1 Physical & Chemical methods of cell disruption 1,2,3 To learn about the
various cell disruption
methods for
intracellular products.
11. 1 Mechanical method of cell disruption – Bead
mill – Ultrasonication – French press 1,2,3
12. 1 Cell disruption by Osmotic shock , detergents 1,2,3
13. 1 Cell disruption by organic solvents , enzymes 1,2,3
To learn the general
theory, equipments,
design and applications
of filtration and
centrifugation
14. 1 Removal of insolubles – theory of filtration 1
15. 1 Rotary vacuum filter – Class work problems 1
16. 1 Flocculation and sedimentation 2
17. 1 Centrifugation- Tubular bowl , Disc centrifuge 1
18. 1 Centrifugation- Basket centrifuge 1
19. 1 Class work problems 1,2,3
UNIT III
FILTRATION, PRECIPITATION AND EXTRACTION
20. 1 Introduction to membrane based separations 1,2,3
To study the theory,
design configuration
and applications.of
Microfiltration &
ultrafiltration.
To understand the
various precipitation
methods, extraction
process.
21. 1 Microfiltration , Ultrafiltration operations 1,2,3
22. 1 Class work problems 1,2,3
23. 1 Precipitation by using salt, solvent and polymers 1,2
24. 1 Extractive separations – Batch and continous 1
25. 1 Class work problem 1,2
26. 1 Aqueous two-phase extraction 3,4
27. 1 Supercritical extraction 3,4
28. 1 Integrated bioprocesing -Insitu product removal 3,4
29. 1 Extractive fermentation 3,4
UNIT IV
CHROMATOGRAPHY AND ELECTROPHORESIS
30. 1 Introduction to product purification methods 1,2
To study the basic
concepts, types, mode
of operation and
applications of
chromatography and
elcetrophoresis
methods.
31. 1 Adsorptive chromatographic separation
processes 1,5
32. 1 High Pressure Liquid Chromatography 2,3
33. 1 Gel permeation chromatography 3,4,5
34. 1 Membrane chromatography 3,4,5
35. 1 Electro chromatography 3,4
36. 1 Electrophoretic separations 3,4,5
37. 1 2D gel electrophoresis 3,4,5
38. 1 Class work Problems 3,4,5
UNIT V
PRODUCT CRYSTALLISATION AND DRYING
39. 1 Crystallisation.-Principles-Nucleation- 1,2
To study the basic
concepts, types, mode
of operation and
applications of
crystallization and
drying
40. 1 Kinetics of batch crystallization – class work
problems 1
41. 1 Batch crystallizers - Scale-up and design 1,2
42. 1 Drying -Principles- Water in biological solids 1,2
43. 1 Heat and mass transfer in drying process 1,2
44. 1 Dryer description and operation- Vacuum shelf
and rotary dryer 2
45. 1 Spray dryer 2
46. 1 Lyophilization-Freeze dryer 2
47. 1 Dryer Design spreadsheet and simulators 2
48. 1 Class work problems 1,2
Text Books
1
Bioseparations: Downstream
processing in Biotechnology
Paul A. Belter, E. L.
Cussler, Wei-Shou Hu
2 Bioseparations science and engineering Roger G. Harrison, Scott R.
Rudge
3 Principles of Bioseparations
engineering
Raja Ghosh
4 Bioseparations: Principles and
Techniques
B. Sivasankar
5 Separation and purification techniques
in biotechnology
Frederick J. Dechow
Name of the Staff Department Email –ID Contact
Number
Signature
Mr.C.Muthukumaran
(Subject Coordinator)
Biotechnology [email protected] 9894595995
Mrs.P.Radha Biotechnology [email protected] 9443599966
Mrs.R.Vasantharekha Biotechnology [email protected] 9566416111
Ms.D.Amala Reddy Biotechnology [email protected] 9677247370
N. Manoj Kumar
Genetic
Engineering
[email protected] 9566138855
s
SRM UNIVERSITY
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING
SUBJECT: FERMENTATION TECHNOLOGY
SEM: VII SEM B.TECH
SUBJECT CODE: BT0411 Year:
2011
LECTURE SCHEDULE
Lecture
Hours Topic / Content
Boo
k Learning outcomes
UNIT I
PILOT PLANT FERMENTATION
1 Introduction about the evolution of fermentation
Technology 1
To understand the
evolution and the current
advancement in
fermentation process such
as use of computers in
parameters control,
Biosnsors and process
optimization in various
cell culture systems
2 Classifications of aerobic fermentation systems 1
3 Application of computer control and sensing
technologies for fermentation process
1
4 Sensors in fermentation 1
5 Bioreactors for rDNA technolgy 1
6 Mammalian cell culture system 1
7 Bioreactors for plant cell, tissue and organ
cultures
1
8 Bioreactors hardware configurations in batch
culture
1
9 Large scale continuous culture 1
UNIT II
FERMENTATION DESIGN
10 Fermentation Department and Equipments 1 To give a orientation
about the fermentation
industry lay out,
instruments and the
design of fermenter for
various culture systems
and to learn optimization
of components as well as
11 Space requirements 1
12 The Design of large fermenter 1
13 Factors to be considered in the design of
fermenter
1
14 Trouble shooting in a fermentation plant- raw
materials; cultures,
1
15 Trouble shooting – Operating procedures, 1
personnel training, lack of maintenance process variables by
RSM 16 General considerations in the maintenance of
fermentation industry
1
17 Statistical methods for fermentation
optimization- Evolutionary optimization
1
18 Response surface method 1
19 Response surface method 1
UNIT III
ENVIRONMENTAL CONCERNS ABOUT FERMENTATION
20 Introduction about regulatory aspects of
environmental concerns about fermentation
1
To give informations
regarding the
maintenance of
biodiversity by reducing
the solid, liquid and
gaseous waste materials
of a fermentation industry
and regulatory aspects.
21 Occupational safety and health act (OSHA) 1
22 Environmental auditing, National
Environmental Policy Act
1
23 Clean water Act 1
24 Waste water treatment strategies 1
25 Organic waste removal, Activated sludge
systems
1
26 Clean air Act 1
27 Control technologies used in air pollution 1
28 Volatile organic compounds emission control 1
29 Particulate control and removal of inorganics 1
UNIT IV
ANAEROBIC DIGESTERS
30 Introduction about anaerobic digesters 2 To know the types of
organisms involved in
anaerobic digestion and
types of products formed
during anaerobic
digestion process, to
study the parameters to
be considered to control
the anaerobic digestion
and to learn how to
control the toxicity in
31 Stages of anaerobic digestion process 2
32 Microorganisms involved in anaerobic digestion
process
2
33 anaerobic respiration process 2
34 anaerobic food chain and fermentation 2
35 Operational conditions of anaerobic digesters 2
36 Steps and scale up of anaerobic digestion
process
2
37 Toxicity 2
38 Types of anaerobic digesters 2
order to increase the
productivity of anaerobic
digestion process
UNIT V
BIOREACTOR FOR PLANT CELL CULTURE
39 Introduction about the biochemical engineering
aspects of plant cell culture 3
To study the equipment
design and process design
of bioreactor for plant
cell cultures, to learn how
to optimize various
control parameters and to
learn various aspects of
large scale plant micro
propagation.
40 Application of plant cell culture in production of
secondary metabolites
3
41 Design and optimization of bioreactor hardware 3
42 Advances in plant bioreactor cultivation process 3
43 Gas concentration effects on secondary
metabolites production
3
44 Integrated bio processing for plant cell cultures 3
45 Study the effect of cell culture on encapsulated
matrices
3
46 Scale up of Bioreactor in plant cell cultures 3
47 Large scale plant micro propagation 3
Text Books
Mr.Y.Ravichandran
Mr. R.Jaiganesh
Mrs.R.Vasantharekha
Mrs.G.Dhanavathy
1
Fermentation and
Biochemical Engineering
Hand Book
Henry C.Ogal
2 The Microbiology of
Anaerobic digesters Michael H. gerardi
3
Advances in Biochemical
Engineering
Biotechnology
T. Sceper and JJ Zhong
B. Tech. program; BT0415 - Stem Cells in Health Care
IV Year/VII Semester ; July to October 2011
Lesson plan
Uni
t
Lecture topics Hour
s
Reference
s
Learning
Outcome
I
Stem cells basics
Definition & introduction to stem cells
1
The basic
knowledge on
stem cells will
Unique properties of stem cells; types of stem
cells
2
Introduction to embryonic, adult and umbilical
cord stem cells
3,4
Important sources of stem cells and creation of
stem cells for research
5,6
1& 2
enable the students
to have an impact
on stem cell
research
Similarities and differences between embryonic
and adult stem cells
7
Properties of stem cells with reference to
potency; different types of potencies- Human
embryonic development – totipotent,
multipotent, pleuripotent etc.
8,9
II
Isolation, characterization and differentiation of
embryonic stem cells:
In vitro fertilization; culturing of embryos
10,11
2& 3
To introduce and
define the
techniques to
isolate embryonic
stem cells and to
differentiate them
into any organ or
tissue. First step
towards stem cell
therapy using
embryonic stem
cells
Isolation of human embryonic stem cells from
embryo
12
Importance of blastocyst and inner cell mass 13
Growing ES cells in lab; laboratory tests to
identify ES cells
14,15
Stimulation ES cells for differentiation
16,17
Properties of ES cells 18
III
Isolation, characterization and differentiation of
adult stem cells:
Properties of adult stem cells; Somatic stem cells
19,20
1&3
Sets out the basics
for isolation and
characterization of
adult stem cells
and paves way for
stem cell therapy
using adult stem
cells
Test for identification of adult stem cells
21
Adult stem cell differentiation
22
Trans differentiation; plasticity
23
Different types of adult stem cells and its
applications
24,25
IV
Stem cell in drug discovery:
Target identification and validation
26,27
4&5
Highlights the
applications of
stem cells in drug
discovery
Manipulating differentiation pathways
(Osteoblasts and fibroblasts)
28
Stem cell therapy Vs cell protection 29
Stem cell in cellular assays for screening drugs 30
Stem cell based drug discovery
31,32
Drug screening and toxicology (cardiotoxicity
and hepatotoxicity)
33,34
V
Genetic engineering of stem cells:
Gene therapy
35
5,6&7
Outlines the
potential
applications of
stem cells in
various therapy
and
thus serves as an
efficient platform
for stem cell
based organ
transplant
Genetically engineered stem cells 36
Stem cells and Animal cloning 37
Transgenic animals and stem cells
38,39
Therapeutic applications of stem cells:
HLA typing; Parkinson disease, Alzheimer's
disease
40,41
Neurological disorder ; limb amputation ; heart
disease
42
Spinal cord injuries; Burns (skin transplantation) 43
Diabetic therapy using stem cells 44
Tissue engineering applications: production of
complete organ - kidney ; eyes
45,46
Organ development using stem cells - heart ;
brain
47,48
References:
1. Adult stem cells by Kursad and Turksen springer 2009.
2. Stem cells handbook by Stewart Sell 2004.
3. Stem cells: from bench to bedside by Ariff Bongso, Eng Hin Lee 2005.
4. Stem cells in regenerative medicine, methods and protocols- Julie audit, William L
Stanford 2009.
5. Stem cells: basics and application by Kaushik D Deb 2009.
6. Principles of tissue engineering- IIIrd
edition by Robert Paul Lanza, Joseph Vacanti
2007.
7. Regenerative medicine stem cells and their applications by K.R.S. Sambasivarao, K.
Anandakrishna IK international publishing house pvt.Ltd. 2010.
Subject handled by
1. Dr.S.Sujatha – subject coordinator – [email protected]
2. Mr.T.Thirumurugan - [email protected]
3. Mr.K.Venkatesan - [email protected]
4. Mrs.G.Dhanavathy - [email protected]
SRM UNIVERSITY
SCHOOL OF BIOENGINEERING
DEPARTMENT OF BIOTECHNOLOGY
Semester: VII
FP0459- Therapeutic nutrition
Lesson plan
Hours Lecture Topics Learning Outcomes
1 Introduction to Therapeutic Nutrition The students will be taught
the nature and scope of
Therapeutic Nutrition. The
important role of proteins,
fats, carbohydrates,
minerals will be discussed
2 Study of importance
3-4 composition and nutritive value of different foods
4-5 Classification, source, requirement, deficiencies, and nutritional
role of proteins
6 nutritional role of fats
7 nutritional role of nutritional role of carbohydrates
8-9 minerals, vitamins, water
10-11 Digestion, absorption and metabolism of proteins An understanding about the
metabolism of various
dietary components.
Importance of BMR
Understanding the energy
value of food
12-13 Digestion, absorption and metabolism of fat and carbohydrates
14 Basal metabolism and factors affecting basal metabolism
15-16 Methods of measurement of Basal Metabolic Rate
17-18 Total energy requirement and factors affecting requirement
19-20 Body composition- Definition, importance, classification, methods
of assessment of body composition
21-22 Energy value of food. Determination of Energy value of food.
23 Introduction to therapeutic diets The fundamental
knowledge on
therapeutic diets will
enable the students
to know the different diet
chart at various stages in
life
24 formulating therapeutic diet from normal diet
25 Diets during pregnancy, lactation
26 Diets during infancy, school age
27-28 Diets during adolescent, adulthood and old age
29-30 Planning for therapeutic diets The dietary management in
case of various diseases
will be discussed in detail.
31-32 dietary management in case of various diseases
33-34 causes, symptoms, physiological changes and dietary management
for metabolic disorders
35-36 physiological changes and dietary management surgical & other
stress conditions
37 Introduction to Malnutrition Outline the effects of
malnutrition and the
various policies by the
38-39 Causes and effect of malnutrition on the vulnerable section of the
society
40 effect of malnutrition on national development government will be
discussed. 41 Measures to combat malnutrition
42-43 National nutrition policy and programmes
44-45 Role of ICDS, WHO, UNICEF, NIN, NFI, CFTRI, FTRI, NNMB
in combating malnutrition
References
Nutritional Biochemistry - Second Edition by Tom Brody, Academic Press, 1998
Manual of Nutritional Therapeutics (Lippincott Manual Series) – 5th
Edition, by
David H Alpers, William F Stenson, Beth Taylor RD, CNSD Dennis M Bier,
2008
Advanced nutrition and human metabolism- by Sareen Annora, Stepnick Gropper,
Jack L. Smith, James L. Groff, Thomson Wadsworth Publishers, 2005
Introduction to Nutrition and Metabolism- 4th
Edition by David A. Bender, CRC
press, 2007
SRM UNIVERSITY
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING
Subject: ENVIRONMENTAL
BIOREMEDIATION
TECHNOLOGY Semester:VII
Subject code: BT 0423 Year: 2011
LECTURE SCHEDULE
S.No. Lecture
Hours Topic / Content Book Learning outcomes
UNIT I
BIOREMEDIATION
1 1
Introduction : Bioremediation- Types of
Bioremediation: Biodegradation-
Biotransformation
1&4,5
To make the student to
learn about the
Bioremediation
Technology to address 2 1 Types of Bioremediation : Insitu 1&4,5
Bioremediation- Exsitu Bioremediation the present day
Environmental
problems.
3 1 Insitu Bioremediation : Bioventing,
Biosparing, Stimulation, Phytoremediation 1&4,5
5 1 Exsitu Bioremediation : Treatment On
Site: Land farming-compositing- biopile-
bioreactor ; Treatment off site :
phytoremediation
1&4,5
6 1 Bioremediation mechanisms : Biosorption-
Bioaccumulation- Reduction-
Solubilization- Precipitation - Methylation
1&4,5
7 1 Case Studies with respect to Land 1&4,5
8 1 Case Studies with respect to water 1&4,5
9 1 Case studies with respect to marine 1&4,5
UNIT II
BIODEGRADATION & BIOCATALYSIS
11 1 Microorganisms involved in
Biodegradation – Comprehensive List –
Strategies and Conditions
1&4,5
To learn the principles
& mechanisms of
microorganisms enzyme
and its applications in
environmental pollution
control.
12 1 Biodegradation strategies for key classes of
compounds- Aliphatic, Aromatic, PAH,
Pesticides,
1&4,5
13 1 Factors affecting biodegradation and its
kinetics 1&4,5
14 1 Biocatalysis: Introduction, Advantages &
Disadvantages 1&4,5
15 1 Enzymes involved in Biotransformation
and Biocatalysis 1&4,5
16 1 Enzyme Hydrolytic Reaction : Esterases,
Proteases, Lipases, Alkaline Phosphtase 1&4,5
17 1 Enzymes Involved in Oxidation/Reduction
Reaction: Laccasse, Hydrogenase and
perooxidase
1&4,5
18 1 Enzymes catalyzing pesticide degradation
reactions : carboxylesterases, 1&4,5
phosphotriesterases
19 1 Kinetics & thermodynamics in
Biotransformation & Biocatalysis 1&4,5
UNIT III
MOLECULAR TECHNIQUES IN BIOREMEDIATION
21 1
Basic Microbial Molecular Biology -
DNA, RAN, and protein-Regulation of
mRNA in transcription in bacteria and
eukaryotes-Restriction enzyme and other
DNA modification enzymes-Cloning
vectors (plasmid, cosmid, fosmid, and
BAC)- Transformation and selection
6
To learn about the
applications of
molecular techniques in
environmental research .
22 1
Molecular fingerprinting techniques (RFLP,
T-RFLP, ARISA, DGGE,
rDNA library, and FISH)
6
23 1 DNA microarray technology in
Bioremediation – Construction of cDNA
Library for microorganisms
6
24 1 Genetically Altered Microorganisms for
field biodegradation of pollutants
6
25 1 Proteomics and its application in
Bioremediation- Fundamentals of protein
and peptide with mass spectrometry-Mass
spectrometry for protein proteome
characterization-2-D gel proteomes-
Metabolomics
6
26 1 Mutagenesis as a genomic tool for studying
gene function- Site Directed Mutagenesis
6
1 Functional Genomic analysis of bacterial
pathogens and environmentally significant
microorganisms -Functional analysis of
environmentally important microbes
6
1 Metagenomics and its application in study 6
of environmentally important
microorganisms
1 Other Novel Techniques - A PCR-based
DNA fingerprinting technique: AFLP for
molecular typing of bacteria- Linking
microbial community structure with
function: fluorescence in situ
hybridization-microautoradiography
and isotope arrays -
6
UNIT IV
BIOTECHNOLOGY FOR HAZARDOUS WASTE MANAGEMENT
27 1 Hazardous waste – Introduction – Liquid,
Gas and Solid wastes- Property;
Characteristics and its toxicity
3
To understand about the
importance of
Hazardous wastes and
its control measures.
28 1 Hazardous waste – Radionuclides –
Treatment and safe disposal 3
29 1 Hazardous waste – VOC ( volatile Organic
Compounds) – Treatment and safe disposal 3
30 1 Hazardous waste – Petroleum Products –
Treatment and safe disposals 3
31 1 Hazardous waste- Biological wastes-
Treatment and safe disposals 3
32 1 Management of Hazardous wastes – Rules
and Regulations; Norms 3
32 1 Case studies with respect to Radionuclides 3
33 1 Case studies with respect to VOC 3
34 1 Case studies with respect to Biological
wastes 3
UNIT V
SPECIAL TOPICS IN BIOREMEDIATION TECHNOLOGY
36 1 Environmental Nanotechnology Research
work in water , air and soil. Green
Nanotechnology: Utilizing Diesel Engine
Exhaust for energy- Magnetic Iron Oxide
Nanoparticles – Carbon Nano tubes
1,2 &4
To understand the recent
advancement in
Environmental
Nanotechnology.
37 1 Phosphatase mediated Bioremediation to
remove toxic heavy metals and
radionuclide’s
1,2 &4
38 1 Bacterial Consortium for Bioremediation 1,2 &4
39 1 Oil and Coal Desulphurization,
Depolymerization & Degradation for value
added products
1,2
1&4
40 1 Biofilm : Mathematical modeling and
simuations studies
1,2 &4
41 1 Biosensor technology for monitoring
pollutants
1,2 &4
42 1 BAP profiling in Biofilms 1,2 &4
43 1 Novel Biomembranes for Bioremediation 1,2 &4
44 1 New Nano products in Bioremediation-
Review
1,2 &4
References
1 Environmental
Biotechnology Allan Scarg
2
Environmental
Biotechnology: Principles
& Applications
Bruce E. Rittman
3 Bioremediation K.H. Herson
4 Environmental
Biotechnology S.N. Jogdand
5
Environmental
Bioremediation
Technology
Rudra Tripathi
Dr. W. Richard Thilagaraj
(Subject Coordinator)
6. Environmental
Molecular Microbiology
Paul A. Rochelle
SRM UNIVERSITY
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING
SUBJECT: BREWING SCIENCE AND PRACTICE SEM: VII
SEM B.TECH
SUBJECT CODE: BT0435 Year: 2011
LECTURE SCHEDULE
Lecture
Hours Topic / Content
Boo
k Learning outcomes
UNIT I
Out line of Brewing
1 Introduction to brewing 1 Students will understand
the processing of beer,
processing of beer, world
wide brewing and beer
statistics and its
applications in the
industries and their great
importance in scientific
research.
2 Malting in outline, Malting Technology, Types
of Kilned malt, Special malt 1
3 Brewing liquors, Milling and Mashing 1
4 Processing of Beer, Types of Beer, World wide
Brewing and Beer statistics, 1,2
5 Malt specification, Adjuncts, Malttun adjuncts,
Copper adjuncts. 1
6 Sources of water, preliminary treatment of 1
water, secondary water treatment. Grades of
water used in breweries.
7 Brewery effluents, wastes and by-products 1
8 Disposal of brewery effluents, preliminary
treatment 1
9 Aerobic treatments of brewery effluents, sludge
treatments and disposal 1
10 Anaerobic and mixed treatments of brewery,
Other water treatments. 1
UNIT II
Science of Mashing
11 Introduction, Mashing schedules 1
To learn about the
Mashing technology and
mashing biochemistry.
To study about the
various compostion of
mashing system, and
milling technology.
12 Altering mashing conditions, grists, malts in
mashing, mashing with adjunts, 1
13 Influences of mashing temperature, Non-malt
enzymes in mashing, mashing liquor and mash
pH
1
14 Mash thickness, extract yield and wort, wort
separation and sparging. 1
15 Mashing biochemistry, wort carbohydrates,
starch degradation in mashing, Non-starch
polysaccharides in mashing
1
16 Proteins, peptides, amino acids and other
substances, miscellaneous substances in
mashing
1
17 Mashing and beer flavor, spent grains,
preparation of grists, principles of milling,
laboratory mills, dry roller milling, impact mills,
spray steep roller milling.
1
18 Mashing technology, mashing in, mash tun,
construction of mash tun, operation of mash tun. 1
19 Temperature programmed infusion, lauter tuns,
strain master, mash filter, choice of mashing and
wort separation.
1
UNIT III
Wort boiling
20 Introduction, principles of heating wort, types of
copper, addition of hop. 1
To study about the wort,
types of copper, boiling
system and control the
substances in wort.
To learn about the
chemistry of wort
boiling, and chemistry of
wort boiling.
22 Pressurized hop-boiling system, low pressure
boiling. Dynamic low pressure boiling,
continuous and high pressure boiling system.
1
23 Control of volatile substances in wort, enegy
conservation and the hop-boil. 1
24 Hot wort clarification, wort cooling, cold break,
wort aeration and oxygenation. 1
25 Chemistry of wory boiling, introduction,
carbohydrates, nitrogenous compound. 1
26 Carbohydrate-nitrogenous constituent
interaction 1
27 Melanoidin, caramel, protein-polyphenol
interaction 1
28 Copper-finings and trub formation. 1
UNIT IV
Wort Fermentation
29 Basic principles of fermentation technology,
ferment ability of wort. Time course for
fermentation, heat output in fermentation.
1
To study about the wort
fermentation and
different fermentation
techniques.
30 Bottom fermentation system, choice, size and
shapes of vessels, operation of cylindroconical
vessels, addition of yeast, control of
temperature, cleaning of vessels.
1
31 Top fermentation systems, vessels and rooms,
operation, Yorkshire square fermentation
system.
1
32 Continuous fermentation system, stirred tank
fermentor, tower fermentaor, continuous
preliminary fermentation.
1
33 Beer maturation and treatment, maturation
flavor and aroma changes, important flavor
changes.
1
34 Dikeltons, sulphur compounds, aldehydes,
volatile fatty acids 1
35 Stabilization against non-biological haze,
mechanism for haze formation, removal of
protein, removal of polyphenol.
1
36 Clarification and filtration, removal of yeast and
beer recovery, beer filtration 1
37 Special beer treatment, low alcohol and alcohol
free beer, ice beers, diet beers. 1
UNIT V
Chemical and physical properties of Beer
38 Introduction, chemical composition of beer,
organic constituents, alcohol and original
extract.
1
To understand about the
chemical composition of
beer and its composition.
To learn about the
packaging methods in
beer production and
labeling the bottle..
39 Carbohydrates, other constituents containing
volatile and non-volatile substances. 1
40 Nitrogenous constituents of volatile and non-
volatile, sulphur containing constituents,
Nutritive value of beer, color of beer.
1
41 Haze, measurement of haze, composition and
formation of haze. Prediction of haze and beer
stability.
1
42 Foam characteristics and head retentions.
gushing 1
43 Practical methods for improving beer stability,
viscosity 1
44 Beer flavor and sensory assessment, flavor taste
and odour, flavor stability. Sensory analysis. 1
45 Packaging, bottling, managing the bottle flow,
secondary packaging, washing, rinsing, full
bottle inspection and labeling.
1
Text Books : Brewing Science and practice. Dennis E. Briggs, Chris A. Boulton, Peter
A. Brookes and
Roger Stevens.
Mr. R. Jai ganesh.
