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ESwab Collection and Transport System Use for aerobic, anaerobic, and fungus cultures, and Strep Molecular tests. Transport temperature: Ambient Stability: Preferably 24 hours, but accepted up to 72 hours after collection for all organisms except Gonorrhea, which is 9 hours ambient or 29 hours refrigerated. Note: Sources included, but not limited to: Wound, Throat, Eye, or Genital. Chlamydia and Gonorrhea by PCR, Female Use for molecular testing for Chlamydia trachomatis and Neisseria gonorrhea from genital sources. One swab is included in the kit and should be placed in the Cobas ® transport tube after collection. The collection swab is marked with a black line for ease of snapping off in transport media. Transport temperature: Ambient or Refrigerated Stability: Samples in Cobas media are stable for 90 days at room temperature or refrigerated. Chlamydia and Gonorrhea by PCR, Female or Male from Non-genital Sources (throat, rectal, oral, etc): Specimens must be placed in Aptima collection tubes for send-out testing. Transport temperature: Refrigerated Stability: 30 days refrigerated Viral Transport Medium Use for virus testing such as Herpes Simplex, Varicella Zoster, Chlamydia Culture or Respiratory Panel by PCR. No wooden shaft or calcium alginate swabs are to be placed in the Viral Transport Media. Transport temperature: Refrigerated Respiratory Panel by PCR Stability: up to 3 days Viral Culture Stability: up to 7 days Dual Swab for Strep Screen Strep screen negative results will be reflexed to Strep A DNA. Collect a dual swab so reflex testing may be performed. Transport temperature: Refrigerated Stability: refrigerated up to 3 days. PapillomaVirus, Chlamydia, and Gonorrhea by PCR ThinPrep PAP vial can be used for PAP testing and all of the above. Lubricant gels should be used sparingly and avoid contact with speculum tip. Gels containing carboners (thickening agents) can interfere with testing. Vigorously agitate collection device in the vial solution. Do not leave device in the vial. Transport temperature: Ambient Stability: 72 hours UA and Urine Culture Transport Transport temperature: Refrigerated Stability: 24 hours unpreserved, 48 hours preserved. Note: The gray tube is the boric acid transport tube for urine culture testing or legionella antigen kit testing. The yellow tube has a preservative and is used for urinalysis testing only. Use the straw to transfer urine into the transport tubes. Transfer urine into the gray tube first, then yellow if using both tubes. Urine Culture Transport Transport temperature: Refrigerated Stability: 48 hours preserved The gray tube is the boric acid transport tube for urine culture testing or legionella antigen kit testing. Use the straw to transfer urine into the transport tube. Note: Cleansing towelettes and sterile specimen cups with blue lid (used for urine chemistry testing and drug screen testing) may be ordered separately. Please consult Atlas test catalog for more information about specimen collection and handling. Chlamydia and Gonorrhea by PCR, Urine, Male or Female Use for molecular testing for Chlamydia trachomatis and Neisseria gonorrhea. Place in Cobas urine transport tube, filling between lines indicated on the collection tube. Transport temperature: Ambient or Refrigerated Collection reminder: Urine should be collected to include the initial urine stream void rather than a mid-stream collection to ensure collection of potential infected epithelial cells. Stability: Samples in Cobas media are stable for 90 days at room temperature or refrigerated. Specimen stability is 72 hours refrigerated prior to placement in Cobas media. Trichomonas - Urine Urine specimen must be placed in Aprima urine specimen collection tube for send-out testing. Transportation temperature: Refrigerated Stability: 30 days refrigerated Stool Culture Gastrointestinal Panel by PCR Use for transport of stool specimens for bacterial culture. For GI Panel by PCR, also collect 1 ml of stool specimen in a sterile specimen cup. The unpreserved sample is needed for a reflex C. difficile toxin test to be run if the PCR test is positive. Transport temperature: Refrigerated Stool Culture stability: refrigerated up to 3 days Gastrointestinal panel by PCR stability: refrigerated up to 4 days Ova and Parasites Use for transport of stool specimens for ova and parasites Transport temperature: Ambient or Refrigerated Stability: 2 months Testing available includes Giardia antigen, Cryptosporidium antigen and microscopic examination for ova and parasites (sent to SHL). Clostridium difficile toxin Unformed stool sample collected in a sterile specimen cup is the only acceptable specimen type. Transport temperature: Refrigerated Stability: refrigerated up to 4 days Fecal Occult Blood Use for transport of stool specimens for occult blood. The sample must be placed in the cassette within 4 hours of collection. If not, the lab can run an occult blood card on stool samples that are less than 24 hours old. Transport temperature: Ambient or Refrigerated Stability: 15 days ambient temperature or 30 days refrigerated when in green cassette. Disclaimer: Collection, transport, and stability are subject to change. Refer to the MercyOne Des Moines Laboratory test catalog for latest collection and testing requirements. Test catalog available at https://mercymedical.elaborders.com/implementation/lwTC.htm Remember to label all specimen containers with patient’s full name, date of birth, and date of collection. MCLB-15831 4/19 Des Moines Laboratory 515-247-4439 877-263-1622 Special Collection Quick Reference Guide

Special Collection Quick Reference Guide

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Page 1: Special Collection Quick Reference Guide

ESwab Collection and Transport SystemUse for aerobic, anaerobic, and fungus cultures, and Strep Molecular tests.Transport temperature: AmbientStability: Preferably 24 hours, but accepted up to 72 hours after collection for all organisms except Gonorrhea, which is 9 hours ambient or 29 hours refrigerated.Note: Sources included, but not limited

to: Wound, Throat, Eye, or Genital.

Chlamydia and Gonorrhea by PCR, Female

Use for molecular testing for Chlamydia trachomatis and Neisseria gonorrhea from genital sources. One swab is included in the kit and should be placed in the Cobas® transport tube after collection. The collection swab is marked with a black line for ease of snapping off in transport media.Transport temperature: Ambient or Refrigerated

Stability: Samples in Cobas media are stable for 90 days at room temperature or refrigerated.

Chlamydia and Gonorrhea by PCR, Female or Male from Non-genital Sources (throat, rectal, oral, etc): Specimens must be placed in Aptima collection tubes for send-out testing.Transport temperature: Refrigerated

Stability: 30 days refrigerated

Viral Transport MediumUse for virus testing such as Herpes Simplex, Varicella Zoster, Chlamydia Culture or Respiratory Panel by PCR. No wooden shaft or calcium alginate swabs are to be placed in the Viral Transport Media.Transport temperature: RefrigeratedRespiratory Panel by PCR Stability: up to 3 days

Viral Culture Stability: up to 7 days

Dual Swab for Strep ScreenStrep screen negative results will be reflexed to Strep A DNA. Collect a dual swab so reflex testing may be performed.Transport temperature: RefrigeratedStability: refrigerated up to 3 days.

PapillomaVirus, Chlamydia, and Gonorrhea by PCR ThinPrep PAP vial can be used for PAP testing and all of the above.Lubricant gels should be used sparingly and avoid contact with speculum tip. Gels containing carboners (thickening agents)

can interfere with testing.Vigorously agitate collection device in the vial solution. Do not leave device in the vial.Transport temperature: Ambient

Stability: 72 hours

UA and Urine Culture TransportTransport temperature: RefrigeratedStability: 24 hours unpreserved, 48 hours preserved.Note: The gray tube is the boric

acid transport tube for urine culture testing or legionella antigen kit testing. The yellow tube has a preservative and is used for urinalysis testing only. Use the straw to transfer urine into the transport tubes. Transfer urine into the gray tube first, then yellow if using both tubes.

Urine Culture TransportTransport temperature: RefrigeratedStability: 48 hours preservedThe gray tube is the boric acid transport tube for urine culture testing or legionella antigen kit

testing. Use the straw to transfer urine into the transport tube.

Note: Cleansing towelettes and sterile specimen cups with blue lid (used for urine chemistry testing and drug screen testing) may

be ordered separately. Please consult Atlas test catalog for more information about specimen collection and handling.

Chlamydia and Gonorrhea by PCR, Urine, Male or FemaleUse for molecular testing for Chlamydia trachomatis and Neisseria gonorrhea.

Place in Cobas urine transport tube, filling between lines indicated on the collection tube.Transport temperature: Ambient or Refrigerated

Collection reminder: Urine should be collected to include the initial urine stream void rather than a mid-stream collection to ensure collection of potential infected epithelial cells.Stability: Samples in Cobas media are stable for 90 days at room temperature or refrigerated. Specimen stability is 72 hours refrigerated prior to placement in Cobas media.

Trichomonas - UrineUrine specimen must be placed in Aprima urine specimen collection tube for send-out testing. Transportation temperature: RefrigeratedStability: 30 days refrigerated

Stool Culture Gastrointestinal Panel by PCRUse for transport of stool specimens for bacterial culture. For GI Panel by PCR, also collect 1 ml of stool specimen in a sterile specimen cup. The unpreserved sample is needed for a reflex C. difficile toxin test to be run if the PCR test is positive.

Transport temperature: Refrigerated

Stool Culture stability: refrigerated up to 3 days

Gastrointestinal panel by PCR stability: refrigerated up to 4 days

Ova and ParasitesUse for transport of stool specimens for ova and parasitesTransport temperature: Ambient or Refrigerated

Stability: 2 months

Testing available includes Giardia antigen, Cryptosporidium antigen and microscopic examination for ova and parasites (sent to SHL).

Clostridium difficile toxinUnformed stool sample collected in a sterile specimen cup is the only acceptable specimen type.

Transport temperature: Refrigerated

Stability: refrigerated up to 4 days

Fecal Occult BloodUse for transport of stool specimens for occult blood. The sample must be placed in the cassette within 4 hours of collection. If not, the lab can run an occult blood card on stool samples that are less than 24 hours old.

Transport temperature: Ambient or Refrigerated

Stability: 15 days ambient temperature or 30 days refrigerated when in green cassette.

Disclaimer: Collection, transport, and stability are subject to change. Refer to the MercyOne Des Moines Laboratory test catalog for latest collection and testing requirements. Test catalog available at https://mercymedical.elaborders.com/implementation/lwTC.htm

Remember to label all specimen containers with patient’s full name, date of birth, and date of collection.

MCLB-15831 4/19

Des Moines Laboratory515-247-4439 877-263-1622

Special Collection Quick Reference Guide