Smart Box - Okolab files/Bold Line Controllers... · Enough for 2 years continuous logging Dimensions, mm ... of the lab, while the other one is introduced into a reference well,

Smart Box

T, CO2 – O2 Bold Line

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Bold Line Controllers

Temperature, CO2 and O2 controllers

The Bold Line is an integrated system of modular controllers, which communicate with each other

through a cableless connector and can be operated via touch screen (OKO-TOUCH) or, with the addition of

the Smart Box web server, even remotely from the PC, tablet or smart phone.

The Bold Line Controllers can be op-

erated as standalone units or in com-

bination with each other through

cableless connection. When a new

unit is attached to an existing one,

the touch screen detects the new

hardware and the new features be-

come immediately available to the

operator.

The dual controller CO2-O2 UNIT BL

[0:10-1-18] is the most common solu-

tion for live cell applications where both CO2 and O2 control are required. A similar solution can be ob-

tained with the combination of the two single gas controllers (CO2 UNIT BL + O2 UNIT BL). This latter con-

figuration is suggested when one of the two controllers is already available and an upgrade is needed to

be able to control the concentration also of the other gas.

CO2 controller with Smart Box T, CO2, O2 controllers with Smart Box

Available Units

Temperature controlles

H201-T UNIT BL for Cage incubator

H301-T UNIT BL for Top Stage Incubator

Gas controllers CO2 % O2 % Pout, atm

CO2 UNIT BL 0 - 18 - 1

O2 UNIT BL [1-20] - 1 - 20 1

CO2 – O2 UNIT BL [0-10;1-18] 0 - 10 1 - 18 1

CO2 – O2 UNIT BL [0-20;1-95] 0 - 20 1 - 95 1

CO2 – O2 UNIT BL - CP 4 - 8 3 - 7 4

OKO - Touch

Oko-Touch

OKO-Touch allows to control the Bold Line Control-

lers in an easy and intuitive way. It connects to any

one of the Bold Line Controllers, detects the equip-

ment available and displays the pages and functions

supported by the equipment detected.

OKO Touch allows to:

• start and stop the controller

• define set point values

• set flow rate of heating air (for Bold Line Cage Incubator)

• define gas cycles (when connected to a gas controller)

• define temperature cycles (when connected to the T controller of Bold Line Top Stage Incubator)

• choose the control mode: specimen or chamber/cage feedback

• select the chamber model and plate adapter (for Bold Line Top Stage Incubator)

• display all the measured variables

• run the self calibration procedure (for Bold Line Top Stage Incubator)

Oko-Touch

Home – T, CO2 and O2 Controllers Status Page – Cage Incubator

Chamber selection – Top Stage Incubator Airflow selection – Cage Incubator

Smart Box

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Smart Box (SM-BL)

Smart Box connects to the highest unit of the Bold Line control system and has the following features:

• data logger

• web server

• video streaming server

Data Logger The Smart Box acquires the status data from the

integrated bold line controllers and stores them in a local not

volatile memory. Examples of acquired and stored data are: set

point temperature, specimen temperature, base, cover and

humidifying module temperature, CO2/ O2 concentration and

set point, gas flow rate, etc. Smart Box also stores maximum

temperature and gas concentration deviation within a certain

time interval. Data can be recalled via web application from

PC, Tablet or Smart Phone.

Web Server

The Smart Box is a web server and can be connected to the

internet via WiFi USB key or via ethernet. If connected, it is

possible to view the current status, and tp control the system

remotely.

Smart Box – front view Smart Box – rear view

Smart Box

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Video Streaming Server

Smart Box includes a web cam which can be connected to the

USB 2.0 Host to provide video streaming.

This is particularly useful to contact the Okolab team for on

line support and to monitor the lab remotely.

Remote assistant

Okolab supports its customers with an online video assistance service. Via Smart Box, the support team

can be enabled to access the integrated controller units to perform maintenance and trouble shooting.

Technical Specifications

Ethernet 10/11 yes

USB 2.0 hosts 2 .(One to Controller, one to webcam or USB Wi-fi key)

USB devices 1

Memory 16 Gb. Enough for 2 years continuous logging

Dimensions, mm 115x115x30

T UNIT for Cage Incubator

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H201 – T UNIT

Temperature controller for Bold Line Cage Incubator

H201 T UNIT BL is the temperature controller used in the Cage Incubator. It keeps the specimen at the

desired temperature by controlling the temperature of the air blown in the enclosure by the Air Heater.

Air Heater The Air Heater recycles the heating air, which enters

into the enclosure through air diffusors.

The Air Blower can be connected to a HEPA filtering

unit. In this way the air that flows in the enclosure is

dust and bacteria free.

The Air Blower is equipped with a flow rate sensor

that allows to measure and control the flow rate of

air blown into the enclosure. This allows to combine

fast start up of the system with smooth operation

during the experiment

H201 –T UNIT (for Cage Incubators) Cage Incubator ensemble view

Air Heater – Double Inlet – Double Outlet recycling system

Air Flow Rate Selection Page

T UNIT for Cage Incubator

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Specimen / Cage Feedback Operation There are two ways to operate the system, depend-

ing on which Temperature sensor is used to provide

feedback to H201 – T UNIT BL. Selection is per-

formed with Oko-Touch.

• Specimen feedback mode: in this configuration, feedback is provided by a sensor placed inside the climate chamber, near the specimen. Simple manipulation is required to stick the thermocou-ple into the reference well with some adhesive tape.

• Enclosure feedback mode: in this configuration, feedback is provided by a sensor measuring the temperature of the air inside the enclosure


T Accuracy ± 0.1 °C

Internal resolution 0.01 °C

Set point value From room to 45°C

Feedback mode Sample / Enclosure Feedback

Heating techology Filtered recirculation of air at controller temperature and flow rate

Filters HEPA and Pre-filters

Dimensions,mm 319x230x117

Air Heater dimensions,mm 294x233x236

SDK Available

Web Operation With Smart Box

Accessories and Codes

H201 T UNIT BL Temperature contol unit - Bold Line. Operated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote operation and remote support

H201-T-FILTER-BOX HEPA filtering box. It attaches to the Air Heater H201-FILTER KIT Filters kit SM-BL Smart Box. Data logger and web server. It allows operation via

web/smart phone/tablet and to receive remote support

Control Mode Selection Page

T UNIT for Top Stage Incubator

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H301 – T UNIT

Temperature controller for Bold Line Top Stage Incubator

H301 T UNIT BL is the temperature controller used in the Top Stage Incubator. It controls the tempera-

ture of the incubating chamber base, of the heated glass cover and of the humidifier collar.

Specimen / Chamber Feedback Operation There are two ways to operate the system, depending on which Temperature sensor is used to provide

feedback to H201 – T UNIT BL. Selection is performed with Oko-Touch.

• Sample feedback: In this configuration, feed-back is provided by an external temperature sensor that reads the temperature of a refer-ence well, placed in the incubating chamber very close to the specimen. The advantage of this configuration is in the accuracy of the tem-perature control. Simple manipulation is re-quired to stick the thermocouple into the refer-ence well with some adhesive tape.

• Chamber feedback: In this configuration, feed-back is provided by a temperature sensor em-bedded into the incubating chamber. A careful calibration performed in our laboratories guaran-tees that specimen temperature is maintained at the desired value. The advantage of this solution is its simplicity of use.

Self Calibration Routine: this routine improves the accuracy of the chamber feedback operation mode.

It employes the two external temperature sensors of the T-Unit. One sensor measures the temperature

of the lab, while the other one is introduced into a reference well, placed in the same position of sam-

ple. The routine is easily launced via Touch Screen and calculates controller parameters to ensure best

accuracy on the position of the sample.

H301 –T UNIT (for Top Stage Incubators) Top Stage Incubator ensemble view

Control Mode Selection Page

T UNIT for Top Stage Incubator

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T Accuracy ± 0.1 °C

Internal resolution 0.01 °C

Set Point value From ambient to 50°C

Feedback mode Sample / Specimen Feedback

Heating technology Electrical heating of metal + heating of conductive glass


SDK Available



H201 T UNIT BL Thermal controller - Bold Line. It allows to work both in cham-ber and specimen feedback Operated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote operation and remote support

SM-BL Smart Box. Data logger and web server. It allows operation via web/smart phone/tablet and to receive remote support

CO2 UNIT BL

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CO2 UNIT BL


Operaration mode Adds CO2 in Air or in N2

Concentration Range 0-18 %

Accuracy ± 5% of CO2concentration, i.e.± 0.25% at 5% CO2

Set point resolution 0.1%

Repeatability Better than 0.1%

Total Flow rate, Nl/m 0.4 constant

Outlet Pressure ambient

CO2 Consumption (at 5% CO2) Nl/min 0.02

CO2 Sensor Non Dispersive InfraRed (NDIR) dual wave length detector

Sensor Life 10 years


Compatible with: any other Bold Line Controller and Okolab Incubator

SDK Available


Filtering device PTFE membrane with 0.2 μm pores


CO2 UNIT BL CO2 Controller - Bold Line. It controls both Air and CO2. CO2 can be regulated in the range 0-18%. Operated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote operation and remote support


OKO-AIR-PUMP-BL Air pump - Bold Line. Plug and play solution for Air inlet. Con-venient alternative to 100% Air tanks/compressed Air lines. It directly connects to Air input of Okolab Bold Line gas controllers and it is operated by the OKO-Touch. Maximum outlet pressure 300 mbar.

O2 UNIT BL [1-20]

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O2 UNIT BL [1-20]


Operaration mode Adds N2 to Air

Concentration Range 0-20%

Accuracy ± 0.1% at 5% O2


Repeatability 0.05% of O2 concentration



N2 Consumption (at 5% O2), Nl/min 0.3

O2 Sensor zirconium oxide

Sensor Life 10 years


Compatible with: any other Bold Line Controller and Okolab Incubator

SDK Available




O2 UNIT BL [1-20] Oxygen Controller - Bold Line. Operated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote opera-tion and remote support



CO2 - O2 UNIT BL [1-10;1-18]

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CO2 –O2 UNIT BL [0-10;1-18]


Operaration mode Adds CO2 and N2 to Air

Concentration Range CO2: 0-10%; O2 1-18%

CO2 Accuracy ± 5% of CO2 concentration, i.e. ± 0.25% at 5% CO2

O2 Accuracy 0.1% at 5% O2


CO2 Repeatability Better than 0.1%

O2 Repeatability 0.05% of O2



CO2 Consumption (at 5% of CO2): Nl/min 0.02

N2 Consumption (at 5% of O2): Nl/min 0.3



Sensors Life 10 years


Compatible with: H201 T UNIT BL and H301-T UNIT BL

SDK Available




CO2 - O2 UNIT BL [0-10;1-18] Combined CO2/Oxygen Controller for Hypoxia - Bold Line. Op-erated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote operation and remote support


OKO-AIR-PUMP-BL Air pump - Bold Line. Plug and play solution for Air inlet. Con-venient alternative to Air tanks/compressed Air lines. It directly connects to Air input of Okolab Bold Line gas controllers and it is operated by the OKO-Touch. Maximum outlet pressure 300 mbar.

CO2 - O2 UNIT BL [0-20;1-95]

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CO2 –O2 UNIT BL [0-20;1-95]


Operaration mode Mixes CO2, O2 and N2


CO2 Accuracy ± 0.3% at CO2=5% CO2 and O2=5%

O2 Accuracy ± 0.2% at CO2=5% CO2 and O2=5%




CO2 Consumption (at 5% of CO2): Nl/min 0.0175

O2 Consumption (at 5% of O2): Nl/min 0.0175

Sensors Digital flow meters, CMOS Sensors


Compatible with: H201 T UNIT BL and H301-T UNIT BL

SDK Available




CO2 - O2 UNIT BL [0-20;1-95] Combined CO2/Oxygen Controller for both Hypoxia and Hyperoxia - Bold Line. Mixes N2, O2 and CO2.. Operated by the OKO-Touch. Can be equipped with the Smart Box for data-logging, remote operation and remote support



CO2 - O2 UNIT BL CP - Multi User System

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CO2 – O2 UNIT BL CP - Multi User System

• It supplies the desired Air-CO2 or N2-O2-CO2 mixture at controlled pressure in the range 1-4 atm

• It replaces pre-mixed vessels or compressed gas lines

• One unit supplies up to 10 microscope incubators

• Mixes two or three gas, according to operator's need

• Pressurized vessel: 5 lt

Technical Specifications Operaration mode Adds CO2 and Air to N2


CO2 Accuracy ± 5% of CO2 concentration, i.e. ± 0.25% at 5% CO2

O2 Accuracy 0.1% at 5% O2


CO2 Repeatability Better than 0.1%

O2 Repeatability 0.05% of O2 concentration

Total Flow rate, Nl/m 0.5, at 4 atm

Outlet Pressure 1-4 atm (absolute pressure)

CO2 Consumption (at 6% of CO2), Nl/min 0.042

N2 Consumption (at 5% of O2), Nl/min 0.5




SDK Available



Accessories and Codes CO2 - O2 UNIT BL-CP Multi-user Bold Line CO2/O2 controller.


SFM Single floating ball flowmeter. Must use one for each incubator

Bold Line Accessories

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Accessories OKO-AP-BL is a plug and play solution to gener-

ate the Air stream needed by Bold Line control-

lers. It is a convenient alternative to air vessels

or to compressed air lines. OKO-AP-BL connects

to Air input of the Bold Line controllers with a 6

mm (O.D.) tube. It is operated via Oko-Touch.

Maximum outlet pressure is 0.3 atm.

Bold Line Accessories

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Okolab S.r.l. OPERATIVE HEADQUARTER Via Olivetti, 1 80078 Pozzuoli (NA), ITALY ADMINISTRATIVE HEADQUARTER Via G. di Prisco, 152 80044 Ottaviano (NA), ITALY Telephone: +39 081 8062624 Fax: +39 081 8764410 E-mail: [email protected]

www.oko-lab.com

Okolab S.r.l. OPERATIVE HEADQUARTER Via Olivetti, 1 80078 Pozzuoli (NA), ITALY ADMINISTRATIVE HEADQUARTER Via G. di Prisco, 152 80044 Ottaviano (NA), ITALY Telephone: +39 081 8062624 Fax: +39 081 8764410 E-mail: [email protected]

www.oko-lab.com

Documents

Smart Box - Okolab files/Bold Line Controllers... · Enough for 2 years continuous logging Dimensions, mm ... of the lab, while the other one is introduced into a reference well,