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Single-Usecustomised fermenter
August 2014
Scalable platform for microbial fermentation
BactoVessel
World first configurable Single-Use-Fermenter
• BactoVesselisavailableinOD110mmsizeforbottomdriveonMagnetic-Stirrer-Table(MST)orintheBio-block
• BactoVesselisavailableinOD130,OD150,OD200withSingle-Use-JacketforHead-Plate-Drive(HPD)orMagnetic-Stirrer-Table(MST)accept-ingmostservomotors
• BactoVesselavailableinOD110,OD130,OD150,OD200,OD250comeswithHead-Plate-Drive(HPD)accept-ingmostservomotors
• BactoVesselissoonavailableinseri-oussize,OD400withStrong-Head-Plate-Drive(SHPD)Configuration
Choose your own design =
fully configurable SUF
Scalable platform =
choose from 2 to >75 litre Vessel Volume
Outstanding performance =
>1,500 rpm capacity
Manufactured entirely from
mono-component rigid plastics
Much more information available on www.cercell.com
Shownafewofthe2,000differentcomponentsfromtheConfigurator Tool abletosatisfyanyrequest
What do I get?
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BactoVessel
Basic specifications for BactoVessel are:• PolycarbonateandNyloncomponents(nomulti-componentplasticfoil!)
• NumberofstandardPG13.5portsaccordingtotheSUFdiameter
• 6differentdiametersand4differentheights
• Turbine(s)forbi-directionalrotation/up-flowcombinedaxial/radialfluidcirculation
• TheSUFbearingsdesigned>1,500rpmforoneweekfermentationprocess
• Baffledvesselforcombinedaxialvortexmixinganddonutshapeflowpattern
•DesignedforNewtonianfluidswithmaxviscosityof50,000cP(similartoyourpreferredstiff,coldgoodmorningyogurt!)
• Designedfor50mBaroperatingpressure
Specification
Much more information available on www.cercell.com
Customised5.7litreSUFforHead-Plate-DrivenexttotheRe-Usable-Jacketforcoolingpriortheinstallation.
Fully configurable BactoVessel´s are created by selecting various optional components via the Configurator Tool:• Severalvesselheating/coolingoptionssuchasintegratedorre-usableheatexchanger
• Variousaerationmethods;microporesparger,microporeL-spargers,ringholespargers,L-spargers,headspacegasexchange
• SeveraldifferentfluidIn&Outmethodsandexhaustmethodstoselectfrom
•Arangeofsensors,Single-Use-Sensor(SUS),liquidlevel/foamsensors
•Optionallyoperatingpressureupto500mBarforOD110,OD130andOD150
•Optionallyoperatingpressureupto500mBarforOD200–OD250requiresacovercageincombinationwithRUJ
• DeliveredhighprecisionE-beam(32kGy)irradiatedindualpolyesterfoilbags
• AllcomponentsinmediacontactareISO10993,FDAapprovedorUSPclassVIcertifiedmaterials.
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BactoVessel facilitate a range of different radial mixing turbine principles: Rushton, Jacob, Smith, Hjort and Bakker:
•OuterDiameter,ODmm:40,50,60,70,80,90,100and120
•Numberofbladesare6or8dependingonprinciple
• Bladeshapes:flat,symmetrichalfcircle,symmetricconcav,asymmetricconcav
• Jacob´sflatbladeangledin75degreefromvertical
Heightofbladesisfixedaccordingtotheturbinediameter.DimensionofturbineandbladedesignedselectablefromtheConfigurator Tool.IfoneormoreaxialmixingMarineimpellersisrequiredintheBactoVesselsuchisavailableinaselectablediameter,3blade,16degreebladeangleddesign.
Two identical BactoVessel OD150 mmintegrating3baffles,ringspargerandRushtonandSmithturbines.Thetwoturbinesperformsverydifferent!
Agitation in BactoVessel Turbines
Unlimited range of turbines
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BafflesBaffles are obstructing vertical arranged vanes or elongated plates inside the vessel needed to stop the radial swirl inside the fermenter and convert the rotational flow to axial mixing.Withoutbaffles,thetangen-tialvelocitiescomingfromanyturbine(s)causestheentirefluidmasstospincreatingacentralvortex.Baffles,sotospeak,increasethefrictiontothevesselinnerwallsurface.BactoVesselbafflesarestraightflatplatesverticallyandperpendicularorientedclosetotheinnersideofthevesselattachedtoandextendingfromthebottomuptoaboveliquidsurface.
NumberofbafflesinBactoVesselrangefrom3to6dependingonthefermenterdiameter.Thebafflewidth(B1)istypicallyT/10–T/12ofthevesselinnerdiameter(T1).BafflesarelocatedwithdistanceofT/72–T/50fromthewall.
OD130 x 420 mm BactoVessel customisedtoend-userrequirementintegrating3baffles,ringsparger,dualIn&Out,HPDdriveandtwospecificRushtonturbines.
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Since oxygen is sparingly soluble in water, it’s one of several growth-limiting factor in fermentations. Thoughdealingwithaerobicmicro-bialapplicationsithelpsthatthecells,bacteria,yeast,etcarealmostinde-structiblefacilitatinghighRPM,suchasupto>1,500RPMwithBactoVessel.
Aeration in BactoVesselThe Rushton 100 % radial flow pattern turbine are a most cost effective design with performance generally limited by pockets or cavities, which cling to the back of the blade at high gas flow rates and/or RPM.Rushtoncausecavitation,considerablestreamliningandpowerdrawreduction.Whengasflowgetstoohigh,thepocketscanbridgebetweenblades,resultinginfloodingandseveremechanicalinstability.RushtonRT8hasthehighestun-gassedPowerNumberofanycommonturbine.
Smithturbinesisahighefficiencyagitatorforveryhighgasdispersionapplications.Charac-terizedbysymmetricalcurvedpipesectionsmountedtoarounddiscresultinginlowPowerNumberandincreasedefficiency.ThisparticularSmithmodelST6hashalftheun-gassedPowerNumbercomparedtotheRushtonturbine.
Ring sparger
Two photosshowaOD150x320mmBactoVesselequippedwiththeadvancedOD60Smith,6bladesturbinedesignplusMarineimpeller,3baffles,1litre/minWV(WorkingVolume)gasinjection.
• At 0 RPM under static condition
• At 1000 RPM. Its worth to notice the limited foam generation even at such high RPM. Head space is in general free of foam
0 RPM
1000 RPM
BactoVessel facilitate aeration / gas injection throughaseriesof0.5mmholesarrangedinacirclebelowtheprimaryturbineintegratedasapartofthevesselbottom.NumberofholesdependingontheturbinediameterorchoicesthroughtheConfigurator Tool.
Much more information available on www.cercell.com
Top class kLa values
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This Smith symmetrically arranged curved (half pipe) blade design from the 1980s is able to handle increased
injected gas volume. CandispersetwicethegascomparedtotheRushtonbeforeflooding.Doesnotexperienceasgreatapowerdrop-offduetogas
loadingcomparedtotheRushtonturbine.Recommendedcombinedwithanup
pumpingMarineimpeller.
Revolutions
ExhaustSingle-Use-Gas-Cooler are available in three sizes and combines inner boro silicate glass tube for excellent heat transfer and outer PC tube and cooling liquid connections. Alltransparenttubesallowtheusertoinspectthefunctionality.
Large size –indesignphase.Max100litre/min/37°gasvolumecapacity.
ConfigurationviatheConfigurator Tool
Small size–ID/OD8/12x270mmglasstube,lowerendinletandoppositeoutletendfittedwithID/OD10/14mmsiliconehose,outletholdingoneormore26cm2surfaceareasterilefilter.Coolingarea68cm2suitableformax2litre/min/37°gasvolumecapacity.
Medium size–ID/OD24/30x300mmglasstube,lowerendinletID/OD18/22mmsiliconehose,andoutletfittedwithID10/14mmsiliconehosepieceand400cm2surfaceareafilter.GlasstubefilledwithRaschig6x6mmglasstubeelements.Coolingarea500cm2suitableformax20litre/min/37°gasvolumecapacity.Coolingliquidin/outletbeingG1/8”forID6mmhoseconnection.
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Sensors and sampling
Sampling
Even the shown photo is a CellVessel bio-reactor the In&Out system for BactoVessel share the same principles and specifications. ViatheConfigurator Tool youcanchooseanycombinationofhoses,connectorsforfluidspassingIn&OutoftheBactoVesselheadplate.AsIn&OutrangeofaccessoriesisbasedonthePG13.5pluganywishispossibletofullfil
Unlimited possibilities in connections
The CellVessel bioreactor use
thesameIn&Outsystem
specificationastheBactoVessel.
Much more information available on
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Single-Use-SensorsBenefits:
• IntegratedSUSreducecontaminationrisk
• Saveshoursofpreptimeandlabor,asnoautoclavingorsterilisationisneeded
• EnablesSUSsetuprightonthebenchtop–nobiosafetycabinet/hoodneededforoperation
•Noautoclavingfacilityisneeded
• ChooseSUSviaConfigurator Tool
Single-Use-Sensors (SUS) offer many advantages
Express
installation
Much more information available on www.cercell.com
Bio mass sensors
• Capacitivesensingtechnologyallowspreciseon-linemonitoringofthecellmass,viablecelldensityaswellascellphysiologicalstate.
• Userscanalsotrackcellcyclechanges,modelapoptosis,andpredictproteintiterallinrealtime
DO Single-Use-Sensor
• Non-invasiveDOwellforVisiFerm™.Pre-installedwithSUScapintegratedtotransparentPCtube.
• ExtendsDOsensorlife,assensorisneverautoclaved
•DOsensordesignedwithdigitalsignalcommunicationthatfitsmostPCS
pH Single-Use-Sensors
• Pre-installed6monthdrystoragetolerantpHSUSsuchasOneFermorFermProbe
• ClassicaldimensionpHsensorwithextendedshelflifetimeofferingmonthsofoperation
• ClassicalpHsignalfitsanyPCS
•Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARCmodule
ARC moule for SUS
• Pre-installedpHSUSOneFermVP6designedforconnectiontoARCanalogue/digitalmodule
• Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARC
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BactoVessel in RUJWater conveying Process-Control-System (PCS) benefit from the RUJ heat exchanger for BactoVessel. TheRUJdesignaddsignificantsupportfacilitatingtheservomotormountedontheheadplate(HPD).RUJisfurtherrequiredforSUFoperatingpressureupto500mBar.
OD150x320 BactoVessel designedwithturbineandimpeller.SUFdesignedformicrobialapplicationwhereexothermalmicrobialreactionrequirescooling.
TheRUJmountedwitha5.7litreVV(VesselVolume)SUFwith2.5litreWV(WorkingVolume)areabletoremoved~20watt/litremediapower.TheRUJallowfastturn-overtimeandhighestpossibleexchangeofenergy.ShownwithRectus21couplingsfordirectconnectiontoBioStatsetoforangehoses.
PreparationBactoVessel SUF weigthislessthan1/10oftheglass/steelSTRofcomparablevolume.AnyonecaneasilytumbletheSUFinasafeway.
The BactoVessel concept is to move weight from the STR, Stirred-Tank-Reactors made from glass / Stain-less Steel to “fixed” components. ThetraditionaljacketedStirred-Tank-Reactorshaswith5.7litreVV(VesselVolume)typicallyaweightof18kilo.Notreallyeasytohandle!
TheBactoVesselSUFiskeptaslightaspossible.The5.7litreBactoVesselSUFweigthislessthan2kilo!Alltheweigthismovedtothefixedcomponent–RUJ!
Reduced
turn-around
time
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Heating & cooling with Re-Usable-JacketFor water conveying Process-Control-System (PCS) CerCell offer a stand-alone Re-Usable-Jacket (RUJ) accessory to insert the SUF. ThisjacketisanefficientheatexchangerandanadvancedoptionforSUFOD110–250mmandin225,320and420mmheight.AflexibleO-ringsealbetweentheremovableSUFtopflangeandsupportringallowsimplereplacementoftheSUF.SuchSUFareequippedwithhead-plateflangeasselectedintheConfigurator Tool.
Specifications for RUJ:
• TheRUJisoneofmanyaccessoriesfoundonwww.cercell.com/commerce
•Operatingtemperature5-60°C
•Maxjacketoperatingpressure400mBar–RUJequippedwithaspringloadedover-pressure,systemprotectionvalvesetat500mBar
• Recommendedwaterflow1litre/minperlitre/SUFWV(WorkingVolume)
• TwoG3/8”femalethreadforIn/Outadapt-ers:OD10barborGL18maleglassthread(similartoUniVesselandothers)orRectus21couplings(similartoBiostatsetup)
•Weightranging5-10kilodependingonsize,theweightfacilitatesstablearrange-mentondesktop.
• Thetransparentrigidplastictubematerialgivesexcellentprocessview.
Water connections
ProudProduction ManagerwithBactoVesselinRUJ
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Fromlefttorigth:Rectus21toG3/8”coupletkit,G3/8”toOD10barbkit,G3/8”toGL18glassthreadkit,hoseadapterstoGL18kits
For water conveying Process-Control-System (PCS) theoptionalintegratedSingle-Use-Jacket(SUJ)heatexchangerisanefficientoptionfortheBactoVesselfermenterfamily.AllowtheuseofMSTupto13.3litreSUFsize.
Specifications:
• SelectedviatheConfigurator Tool
•Operatingtemperature5–60°Cheating/coolingwater
• Recommendedmaxoperatingpressureis500mBar
• Burstpressureat20°Cis<1Bar
• Recommendedcoolingflow1litre/min/WV(WorkingVolume)
• StandardtwoG3/8”femalethreadcon-nectionsonjacket
• Adaptersavailableasaccessory:G3/8”toOD10barb,G3/8”toGL18maleglassthreadadapterlikeUniVessel,G3/8”toRectus21male/femalekit
• Thetransparentrigidselfsupportiveplas-ticmaterialgivesexcellentprocessview.
Heating / cooling with Single-Use-Jacket
16.4 litre VV (Vessel Volume) CellVessel (OD250x320) viaConfigurator Tool equippedwithSUJ.ThetwofemalethreadedG3/8”connectionsarevisibleontherightsideoftheSUF.
Water connection
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BactoVesselequippedwithSIngle-Use-JacketandfemaleG3/8”threadedconnectionsforavarietyofadapters.
BactoVessel with SUJ
5.7 litre VV (Vessel Volume) BactoVessel viaConfigurator ToolequippedwithSUJ
1. FHC–Flexible-Heating-CoolingelementwrappedaroundtheSUFwith5.5litreVV(VesselVolume),2.5litreWV(WorkingVolume),removedpower~5watt/litremedia
2. AluminiumRHScoilencapsulatingtheSUFwith5.5litreVV,2.5litreWV,removedpower~10watt/litremedia
3. IntegratedSingle-Use-JacketonSUFwith5.5litreVV,2.5litreWV,removedpower~20watt/litremedia
4. IntegratedjacketonUniVesselSTR–glass/steel,removedpower~25watt/litremedia
Heat exchange
Cooling
as with
glass
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HPD drive• Head-Plate-Drive(HPD)forBactoVessel®SUFoffercoupling/adapterkitstoservomotorsfromApplikon®,Biostat®,DASGIP®,BJ,NBS,Solaris,Finesse®,BBI-biotechandotherPCSservomotors
• Re-usableservomotoradapterseasilyattachedtotheOD30mmHPDcentrebearingontheBactoVesselheadplate
• Forhighloadsamotorstandishelpful
BactoVessel 5.7 litrefacilitatingSmithturbineandMarineimpelleroperatingat1,500RPMmountedinRe-Usable-Jacket,RUJequippedwithservomotorsupportandSky-Supportforthegas-coolerandexhaustfilter.
Agitation methods
Kollmorgen AKM23DservomotordrivingviaHPDinBiostatsetupa5.7litreBactoVesselsubmersedinaRUJ
Use you
own PCS
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MST drive•Magnetic-Stirrer-Table(MST)StainlessSteelconstructionfeaturinglonglifeandsilentrunningtoothedrubberbeltonaluminiumsprockets,twolifetimesealeddouble-rowballbearings
•MSTrotor(undertheSUForSUB)isequippedwith12permanentmagnetsandabletotransfer>100wattpowerdependingontheparticularservomotorcapacity
• Dim:390x180x50mm,weightis5,7kilo
• ForBactoVesselwithSIngle-Use-Jacket,SUJ
MSTdrivinginthiscasea3.0litreCellVesselbioreactor.FullyindependentoftheparticularPCSservomotor.TheMSTadapter,seentotheleft,takescareofthisisssue.
Kollmorgen AKM23D servomotordrivingMSTinBiostatsetup
Much more information available on www.cercell.comMuch more information available on www.cercell.com
Mostservomotorsfacilitatetheseadapters.
You get drive options
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SUF productsOur “Configurator Tool” can help you design a SUF to your User-Requirement-Specification.
BactoVessel sizes to select from
Vessel diameter, OD mm 110 130 150 200 250 400
Number of PG13.5 ports 7 7 9 10 10 12
Turbine diameter, OD mm 40-50 50-60 50-70 80-100 100-120 100-150
Vessel Volume, litre
225 mm 2.1 3.0 4.1
320 mm 4.2 5.7
420 mm 13.3 21.0
500 mm
600 mm 29.5 75.6
Thank you for your time
browsing this leaflet
You get our attention
Much more information available on
www.cercell.com
CerCellApsMalmmosevej19C
DK-2840HolteDenmark
www.cercell.comphone+4545423393
Specificationssubjecttochangewithoutnotice.Pleaserefertowww.cercell.com/intellectual-property-rights
regardingIPR,registrationsandtrademark.