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Single Particle Cryo -EM. Wen Jiang. http:// jiang.bio.purdue.edu. Markey Center for Structural Biology. Cryo -EM: Imaging & 3 -D Reconstruction. Imaging ( 3D 2D ). Reconstruction ( 2D 3D ). Single Particle Cryo - EM: Jiang Group @ Purdue. West Nile Virus. 20 Å. 10Å. 7 Å. 5Å. - PowerPoint PPT Presentation
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Single Particle Cryo-EM
Wen Jiang
Markey Center for Structural Biology
http://jiang.bio.purdue.edu
Cryo-EM: Imaging & 3-D Reconstruction
Imaging (3D2D)
Reconstruction(2D3D)
7Å 10Å 4Å 3Å 3.5Å 5Å 20Å
P22 phage
West Nile Virus
T7 capsid IIe15 phage
T7 capsid I
resolution
T7 capsid I (asym)
e15 phage (asym)
Single Particle Cryo-EM: Jiang Group @ Purdue
Circovirus (PCV2)
Tulane Virus
DNA nano-particles
Computing Needs for Cryo-EM
Projection
2-D Alignment
3-D Reconstruction
Image Processing Tasks
Desktop
Group of Desktops
Cluster
Condor
Computing Resources
?
http://diagrid.org/tools/cryoem
Cryo-EM on DiaGrid: Benefits
http://diagrid.org/tools/cryoem
• pre-configured installation. As a user, you don’t need to worry about software download and installation. Just focus on your science projects.
• rapid dissemination of new algorithms. Newly developed image processing algorithms can be installed here to immediately benefit all user projects.
• large computing resource. The computational resources for image processing/3-D reconstruction tasks are provided at no cost to users by DiaGrid which leverages the vast amount of computing resources at Purdue and its partner institutions.
http://diagrid.org/tools/cryoem
Demo