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Membranes for Life Science Research

2019 Catalog and Price List

SiMPore Inc. Precision Membrane Technologies

SiMPore.com SiMPore Inc. 2

2019 Membranes Catalog

Ordering Information Hours of Operation SiMPore Inc. maintains regular business hours of 9:00 am to 5:00 pm Eastern Standard Time, Monday – Friday. To place an order, request a catalog, or initiate a technical service request after business hours, please visit our website or write to us via email.

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Purchase Orders To place an order using a purchase order, please forward it to us at the following;

Email: [email protected] Fax: +01 (888) 249-2935.

Technical Assistance and Other Inquiries For technical or other assistance, please email [email protected] or call during regular hours of operation at +01 (585) 214-0585 or +01 (888) 323-NANO.

Mailing Address SiMPore Inc. 150 Lucius Gordon Drive, Suite 110 West Henrietta, NY 14617 USA

Web Address http://www.simpore.com

Pricing and Terms of Sale All prices are listed in US dollars and are valid as of November 1, 2019. Prices are subject to change. Terms of Sales are available upon request or online at our webstore.

Shipping SiMPore membrane products are shipped via two-day service via FedEx for shipments within the US. International shipments are usually shipped via Priority International or similar service offering. Contact us before ordering if a different shipping service is requested.

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Table of Contents Ordering Information & Terms of Service 2

Table of Contents 3

Membranes for Cell Culture Applications 4

Membranes for Assays & Filtration Applications 8

Membrane Gaskets 11

Membrane Accessories 13

Custom Membrane Products 14

Membrane Information 15

Membrane Product Numbers 17



Membranes for Cell Culture Applications Nanomembranes for Cell Culture Applications Specifications SiMPore’s nanomembranes for cell culture encompass a suite of ultra-thin materials offering superior permeability and industry-leading optical properties. These properties enable a wide array of cell culture experiments and are particularly well suited to barrier function, cell transport and migration, live cell imaging, and mechanism of action studies. A survey of key attributes for our membrane products for cell culture is provided in the table below.

These properties enable a wide array of cell culture experiments (see Select Publications below) and are particularly well suited to barrier function, cell transport and migration, live cell imaging, and mechanism of action studies.

Nanomembranes are available in an easily handled chip format (5.4x5.4x0.3 mm) that can be integrated into microfluidics and 24-well plates with available fixturing gaskets (see below).

A comparative imaging study between SiMPore nanomembranes and conventional track-etched polycarbonate membranes widely used in cell culture is provided below. From left to right, the images highlight the exceptional thinness of SiMPore nanomembranes, its superior optical properties in both phase and fluorescence imaging modes, and the utility for establishing in vitro barrier models.

Product Pore Size Thickness Assay Transparency Pore

Density Porosity Intrinsic Permeabity

NPN 60 nm 100 nm Permeability Glass-like 1 x 1010

cm

-2

20% 0.1 cm/sec

Dual Scale

(pending)

60 nm and

3 µm 100 nm Permeability and

Transmigration Glass-like 1 x 1010

cm

-2

25% > 0.1 cm/sec

SiN 0.5 µm 400 nm Permeability Excellent 1 x 108

cm-2

5%, 20% 0.03 cm/sec

G-FLAT™ Oxide

0.5 µm - 8.0 µm 300 nm Permeability and

Transmigration Excellent 1 x 108

cm-2

5%, 20% 0.03 cm/sec



Nanoporous Silicon Nitride Membranes

• One (0.7x2 mm) or two (0.7x2.5 mm) slot membrane on each chip afford a large area for viewing 1,000s of cells.

• Highly permeable membranes support cell-cell communication in co-cultures and establishment of polarized cell monolayers.

• Patented (US Patents #9,789,239 and #10,391,219) nanomembrane enabling unique cellular assays with imaging end-points.

Part Number Part Name Pore Size & Porosity Price (10-pack)

NPSN100-1L Nanoporous Silicon Nitride 100 nm thick Membrane Chips (1 slot)

50 nm pores, 20% porosity

$375

NPSN100-2L Nanoporous Silicon Nitride 100 nm thick Membrane Chips (2 slots)

50 nm pores, 20% porosity

$375



Microporous Silicon Nitride Membranes • Three (0.7x3 mm) slot membranes per chip affords

a large working area for a variety of cell culture applications.

• 400 nm thick silicon nitride membranes offer robustness for demanding microfluidic applications.

• Highly permeable membranes (20% porosity) support permeability and transport cellular assays.

Microporous G-FLAT™ Porous Glass Membranes

• Large (2x2 mm) square membrane on each chip with a thin (300 nm) membrane provides sufficient working area for viewing 1,000s of cells.

• Glass-like membranes offer unparalleled optical transparency in a permeable, cell-friendly support.

• Highly permeable membranes (20% porosity) support permeability and transport cellular assays with 0.5 µm diameter pores.

• Choice of membranes with 5% porosity and 0.5 µm diameter pores for cell-cell communication studies or 3.0 and 8.0 µm diameter pores for cellular migration assays.

• Made using SiMPore’s patented process (US Patent #9,945,030).


MPSN400-3L-0.5HP

Microporous Silicon Nitride 0.5-micron Membrane Chips (high porosity)

0.5 µm diameter pores, 20% porosity $375


GFLAT300-1Q-0.5HP

Microporous G-FLAT™ 0.5-micron Membrane Chips (high porosity)


GFLAT300-1Q-0.5LP

Microporous G-FLAT™ 0.5-micron Membrane Chips (Low porosity)


GFLAT300-1Q-3.0LP



GFLAT300-1Q-5.0LP



GFLAT300-1Q-8.0LP





Hybrid MicroNano Membranes

• One (0.7x2 mm) or two (0.7x2.5 mm) slot membranes on each chip afford a large area for viewing 1,000s of cells.

• Similar to 100 nm thick Nanoporous Silicon Nitride, but with additional 3.0 µm diameter pores throughout the membrane.

• Ideally suited for cellular migration studies under flow conditions in microfluidics.

• Highly permeable membranes support cell-cell communication in co-cultures and establishment of polarized cell monolayers on membranes mimicking extracellular matrices

Select Cell Culture Publications Featuring SiMPore Membranes

• Mossu et al. (2019) A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood–brain barrier under flow. J. Cerebral Blood Flow Metab. 39, 395-410.

• Salminen et al. (2019) Ultrathin Dual‐Scale Nano‐and Microporous Membranes for Vascular Transmigration Models. Small 15, 1804111.

• Carter et al. (2017) Ultrathin transparent membranes for cellular barrier and co-culture models. Biofab. 9, 015019.

• Mazzocchi et al. (2015) Porous Membranes Promote

• Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions. Cell. Mol. Bioeng. 7, 369-378.

• Nehilla et al. (2014) Endothelial vacuolization induced by highly permeable silicon membranes. Acta Biomater. 10, 4670.

• Agrawal et al. (2010) Porous nanocrystalline silicon membranes as highly permeable and molecularly thin substrates for cell culture. Biomaterials 31, 5408-5417.


NPSN100-MP-1L-3.0LP

Hybrid MicroNano Silicon Nitride 100 nm thick Membrane Chips (1 slot)

50 nm + 3 µm pores, ~25% porosity Upon request

NPSN100-MP-2L-3.0LP

Hybrid MicroNano Silicon Nitride 100 nm thick Membrane Chips (2 slots)

50 nm + 3 µm pores, ~25% porosity Upon request



Membranes for Assays & Filtration Applications Nanomembranes for Microfluidic Assays and Filtration SiMPore offers a range of nanomembranes with nanopores, micropores, or microslits that are ideally suited for assay development and filtration-based isolations.

Nanomembranes are available in an easily handled chip format (5.4x5.4x0.3 mm) that can be integrated into microfluidics via UV-ozone or oxygen plasma bonding techniques using available fixturing gaskets (see below). NPN Membranes

• Four 0.3x3 mm membrane windows balance mechanical strength and membrane surface area.

• Nanometer cut-off (50 nm average pore diameter) and high permeability (20% average porosity) enable unique experimental and assay capabilities using this patented nanomaterial (US Patents #9,789,239 and #10,391,219).

Silicon Nitride Microporous Membranes

• Three (0.7x3 mm) slot membranes on each chip affords a large working area for a variety of assay and filtering applications.

• 400 nm thick silicon nitride membranes offer robustness for demanding microfluidic applications (membranes withstand ≥ 15 PSI differential pressure).

• Highly permeable membranes (20% porosity) support low pressure drop operating conditions within microfluidic devices.

• Choice of cut-offs at 0.5, 3.0, or 8.0 µm diameter pores support a variety of assay or experimental capabilities.


NPSN100-4L Nanoporous Silicon Nitride 100 nm thick Membrane Chips (4 slots)

50 nm pores, 20% porosity $375



Silicon Nitride Microslit Membranes

• Standard 3 membrane format affords a large working area

• Innovative rectangular prism (50 µm length) pore geometry which resists fouling and cake formation during filtration

• Robust 400nm thick SiN membrane material demonstrates superior mechanical properties relative to other nanomembranes

• Slit widths of 0.5, 1.0, and 8.0 microns at 10% porosity

Select Filtration and Microfluidics Publications

• Masters et al. (2019) An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis. Nanomed. Nanotech. Biol. Med. 21, 102039.

• Chung et al. (2014) Highly permeable silicon membranes for shear free chemotaxis and rapid cell labeling. Lab Chip 14, 2456.

• Johnson et al. (2013) Ultrathin silicon membranes for wearable dialysis. Adv Chronic Kidney Dis 20, 508-515.

• Snyder et al. (2011) An experimental and theoretical analysis of molecular separations by diffusion through ultrathin nanoporous membranes. J Memb Sci 369, 119-129.


MPSN400-3L-0.5HP



MPSN400-3L-3.0HP



MPSN400-3L-8.0HP




MSSN400-3L-0.5 Microslit Silicon Nitride 0.5 µm Membrane Chips

0.5 µm slits, 10% porosity $375







• Gaborski et al. (2010) High-performance separation of nanoparticles with ultrathin porous nanocrystalline silicon membranes. ACS Nano 4, 6973-6981.

• Striemer et al. (2007) Charge- and size-based separation of macromolecules using ultrathin silicon membranes. Nature 445, 749-753.



Membrane Gaskets

Silicone gasket kits are pre-cut sheets designed to assist you in better-integrating SiMPore nanomembrane chips into your cell culture or microfluidic system. The gaskets are made from platinum-cured polydimethylsiloxane (PDMS) with 40° shore A hardness, ability for elongation up to 600% and glass-like translucency. These gaskets can be UV-Ozone, Corona discharge or oxygen plasma activated for layer-by-layer assembly of nanomembrane chip-incorporating devices. All pre-cut PDMS is left sandwiched between protective liners for maintaining integrity until liners are removed prior to use.

Flow Channel Gasket Kit • Glass-like translucent PDMS gaskets enable flow

cell applications. • 250 µm thick gasket layers ensure low channel

volumes. • Pre-cut for mounting on standard glass coverslips • Kits include pre-cut gaskets for 10 flow devices, 4

pack of luer-lock 20-gauge needles, cover slips, and instructions for assembly.

Multi Well Gasket Kit

• Glass-like translucent PDMS gaskets allow establishment of static well applications.

• 1.6mm gasket layers provide ample apical and basal well volumes.

• Designed to fit standard 24-well plates used for tissue culture.

• Kits include pre-cut gaskets for 10 multi well devices and instructions for assembly.

Translucent PDMS Sheets • Cast PDMS with glass-like translucency pre-cut to

a 200 mm x 200 mm square sheet. • 40° shore A hardness • Each PDMS sheet is protected by a thick backer

stock (PE) and surface liner (PET) • Choice of sizes include 0.25, 0.5, 0.8, 1.6, and 3.2

mm thickness material • Available as sheets of 1 or packs of 5



Membrane Gasket Part Numbers

Part Number Part Name Price (USD)

GASKET-CS Chip Mounting Gaskets for Cover Slips (Kit for 10 Chips) $25

GASKET-MW Chip Mounting Gaskets for 24-Well Plates (Kit for 10 Chips) $25

GASKET-250 Pre-cut PDMS Sheet 250 µm thick (1 sheet) $25



GASKET-1600 Pre-cut PDMS Sheet 1,600 µm thick (1 sheet) $50

GASKET-3200 Pre-cut PDMS Sheet 3,200 µm thick (1 sheet) $80

GASKET-250PK Pre-cut PDMS Sheet 250 µm thick (5 sheets) $100



GASKET-1600PK Pre-cut PDMS Sheet 1,600 µm thick (5 sheets) $225

GASKET-3200PK Pre-cut PDMS Sheet 3,200 µm thick (5 sheets) $375



Membrane Accessories Gel-Box with 5x5 Grid Labeling

• Gel-Pak® Brand box permits convenient chip storage and inspection via see-through lid.

• Numbered grid with high-contrast backing film

• Labeled with 5x5 grid (rows A-E and columns 1-5).

• Once placed on low-tack gel, membrane chips are firmly held in place but can be easily removed with recommended K6-style tweezers.

Gel-Box with 5x5 Grid Labeling • Accommodates up to fifty (50) 5.4mm x 5.4mm x

0.3mm membrane chips

• Made from precision-machined hard-anodized aluminum.

• Chip holder can be removed from polystyrene case for membrane chip cleaning to remove organic contaminants and/or activation by oxygen plasma, UV-ozone or Corona wand.

Plastic Membrane Handling Tweezers • Carbon-fiber Tweezers prevent damage to

membrane chips during handling. • Format is standard K6-Style (length: 120 mm; gap:

5.5 mm) and ESD safe.

• Square tip style increases control of membrane chips when handling.

Membrane Accessories Part Numbers

Part Number Part Name Price (USD) GELBOX Gel-Boxes with 5x5 grid labeling (Pack of 5) $50

HOLDER Reusable Anodized Aluminum Holder for Porous Membrane Chips $225

K6TWZR K6 style, flat edge, non-static tweezers Membrane Chips $17



Custom and OEM Membrane Fabrication Services Custom Membranes for Microfluidics

With over a decade of experience in silicon processing methods, SiMPore offers a wide range of services to meet your customized membrane needs. Our process engineers and research scientists look forward to working with you in developing a custom membrane product that meets the needs of your next development project. We also can supply customized membranes as OEM components for your product development efforts

We offer an array of customization options, ranging from alternative membrane formats to fully integrated devices. Additionally, we have unique expertise in surface modification and specialty coatings for all our membrane products. Please send your inquiries to [email protected].




Membrane Information Silicon Nanomembranes

SiMPore’s suite of silicon nanomembranes enable novel research and experimental and assay capabilities in the life sciences and materials development industries.

Nanoporous Silicon Nitride (NPN) SiMPore’s innovative nanoporous silicon nitride (NPN) provides unparalleled permeability, optical transparency, and precision pore sizes – all in the same membrane material. Through a patented manufacturing process, nanopores are randomly created within a Silicon Nitride (SiN) layer of nanometer thickness. This process produces a very high pore density and thus highly permeable membranes. These NPN membranes are atomically flat and chemically inert, enabling markedly reduced biofouling and nonspecific interactions across a wide array of sample types.

Microporous and Microslit Silicon Nitride (SiN) SiMPore fabricates microporous and microslit membranes within low-stress silicon nitride layers deposited on prime-grade silicon wafers. These porous features are uniformly, photolithographically patterned and etched. Current products offer 10-20% porosity in a robust 400 nm thick membrane for demanding flow-based applications.

Microporous G-FLAT™ Silicon Dioxide SiMPore’s microporous G-FLAT™ silicon oxide membranes are manufactured with a patented method, yielding a flat, porous and glass-like layer. Current membranes are available with 5-20% porosity in a 300 nm thick membrane that is optically transparent like glass. These products are also directly patterned using photolithography, resulting in uniform pore sizes.



Common Membrane Chip Dimensions

Currently available nanomembrane chips feature a 5.4x5.4 mm, 310 µm thick silicon frame. Specific formats and related dimensions for various membrane thicknesses and membrane surface area per chip may be found in the reference table that follows. The number of membranes per chip, as well as the Membrane thickness (t), will vary depending on membrane product type.

Dimension Type Dimension ID

Membrane Window Format (Dimensions in um)

1 Slot 2 Slot 3 Slot 4 Slot 5 Slot 1 Square

Frame Thickness D 310

Window Width W1 700 700 700 300 150 1000 2000

Membrane Window Length L 2000 2500 3000 3000 3000 1000 2000

Frame Window Width W2 1138 1138 1138 738 538 1438 2438

Edge Margin (Sides) d1 2300 1625 1100 1100 1500 2200 1700

Window Gap d2 N/A 750 500 625 450 N/A N/A

Membrane Surface Area A 1.4mm2 3.5 mm2 6.3 mm2 3.6 mm2 1.5 mm2 1 mm2 2 mm2

Example Applications Cell Culture, Flow Cells, Microfluidics

Dead-End Filtration, Microfluidics Cell Culture



Membrane Product Numbers

Membrane Type

Part Number Product Name Membrane

Thickness Average Pore Size & Porosity

Window Pattern / Format

Price (USD)

Nanoporous Silicon Nitride (NPN)

NPSN100-1L

Nanoporous Silicon Nitride 100 nm thick Membrane Chips (1 slot)

100 nm 50 nm pores, 20% Porosity

One membrane: 0.7x2 mm

$375 (10-pack)

NPSN100-2L

Nanoporous Silicon Nitride 100 nm thick Membrane Chips (4 slots)

Two membranes: 0.7x2.5 mm

$375 (10-pack)

NPSN100-4L

Nanoporous Silicon Nitride 100 nm thick Membrane Chips (4 slots)

Four membranes: 0.3x3 mm

$375 (10-pack)

Microporous Silicon Nitride (SiN)

MPSN400-3L-0.5HP

Microporous Silicon Nitride 0.5 µm Membrane Chips (high porosity)

400 nm

0.5 µm pores, 20% porosity

Three membranes: 0.7x3 mm

$375 (10-pack)

MPSN400-3L-3.0HP



$375 (10-pack)

MPSN400-3L-8.0HP



$375 (10-pack)

G-FLAT™ Porous flat silicon oxide

GFLAT300-1Q-0.5HP

Microporous G-FLAT™ 0.5 µm Membrane Chips (high porosity)

300 nm


One membrane: 2x2 mm

$375 (10-pack)

GFLAT300-1Q-0.5LP

Microporous G-FLAT™ 0.5 µm Membrane Chips (Low porosity)


$375 (10-pack)

GFLAT300-1Q-3.0LP



$375 (10-pack)

GFLAT300-1Q-5.0LP



$375 (10-pack)

GFLAT300-1Q-8.0LP



$375 (10-pack)

Microslit Silicon Nitride (SiN)

MSSN400-3L-0.5

Microslit Silicon Nitride 0.5 µm Membrane Chips 400 nm

0.5 µm slits, 10% porosity Three

membranes: 0.7x3 mm

$375 (10-pack)

MSSN400-3L-1.0

Microslit Silicon Nitride 1.0 µm Membrane Chips

1.0 µm slits, 10% porosity

$375 (10-pack)



MSSN400-3L-8.0

Microslit Silicon Nitride 8.0 µm Membrane Chips

8.0 µm slits, 10% porosity

$375 (10-pack)

Accessories

GELBOX Gel-Boxes with 5x5 grid labeling (Pack of 5) - - - $50

HOLDER Reusable Anodized Aluminum Holder for Porous Membrane Chips

- - - $266

K6TWZR K6 style, flat edge, non-static tweezers Membrane Chips

- - - $17

GASKET-CS

Chip Mounting Gaskets for Cover Slips (Set for 10 Chips)

- - - $25

GASKET-MW

Chip Mounting Gaskets for 24-Well Plates (Set for 10 Chips)

- - - $25

GASKET-250

Platinum Cured Transparent Silicone sheet, top & bottom liners, 400 cm^2

250 µm

nonporous 200 mm x 200 mm

$25

GASKET-500 500 µm $25

GASKET-800 800 µm $28

GASKET-1600 1600 µm $50

GASKET-3200 3200 µm $80

GASKET-250PK

Platinum Cured Transparent Silicone sheet, top & bottom liners, 400 cm^2, Pack of 5 Sheets

250 µm $100

GASKET-500PK 500 µm $100

GASKET-800PK 800 µm $125

GASKET-1600PK 1600 µm $225

GASKET-3200PK 3200 µm $375

Documents

SiMPore Inc. - TEMwindows.com · Email: [email protected] Fax: +01 (888) 249-2935. Technical Assistance and Other Inquiries For technical or other assistance, please email [email protected]