Si Nitride Chip.

4.4 mm

4.4 mm Window

unmodified silicon nitride window/chip as supplied by Adam Hall (Norcada NX4402x)window is 30nm nitride, it is 10-20um (these look like 20um) square in a frame of 4.4mmx4.4mm 200um thick Si

http://www.norcada.com/nitride_window_xray.php - all image areas were intended to overlap or fall closely adjacent to window

samples returned by Adam Hall - pores and origami (AH1-4) or just pores (AH5a and 5b)AH1 5x5, 400 nm sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2 (this is

the source of the sample trace and of the one AFM image I sent)AH2 5x5, 400 nm sep, origami sample 2, measured in 50 mM KCl w/ TE and MgCl2AH3 5x5, ~2.5 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2AH4 5x5, ~1.6 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2AH5a no origami 400nm sepAH5b no origami 2.8um sep

CO samples from Marshall - origami sample 1-4 Sample 1 contains 1.7 nM origami (12ng/ul) with no extra tail extender

Sample 2 contains 1.7 nM origami (12ng/ul) plus 200nm tail extension at about 1:1 ratio

Sample 3 contains 1.7 nM origami (12ng/ul) plus 1200nm tail extension at about 1:1 ratio with origami

Sample 4 contains ~10nM origami with 5x extra staples - this is unpurified so should have longest shelf life

Adam Hall (AH) Samples 1, 2, and 5a. 400-nm nanopore spacing.

Adam Hall (AH) Sample 4. 1.6-µm nanopore spacing.

20 µm

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 - unrinsed, as recieved

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals - to be continued

approx., location of new zoomed scan below

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals – images follow

AH4 - off window

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 - rinsed

AH4 - off window

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 -rinsed

AH4 - at window

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 rinsed

AH4 - on window – no array visible

Adam Hall (AH) Samples 4 1600-nm nanopore spacing.

AH4 -rinsed

AH4 - on window - tried for dead center of window