Fragmentation via Roche Nebulization

Fragmentation via Covaris shearing (with microTUBES)

Genomic DNA

5’A

End-repair and 3’ A-tailing

PE Adapters Ligation to DNA Fragments

5’5’

5’5’

Size-Selection of DNA Fragments

PCR Enrichment of Adapter-Modified DNA Fragments

Agilent DNA 1000 Profile of Roche Nebulized DNA

Agilent DNA 1000 Profile of Covaris-Sheared DNA

5’A

3’

3’

3’

3’PE primers