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Separation ScienceTODAY
a newsletter for today’s chromatographer
© Copyright 2004 Alltech Associates, Inc. UNP
Specialists in ChromatographySpecialists in Chromatography
SST VOLUME 4 brochure #489K
Page 12 & 15
I N S I D E
INDUSTRY FOCUS:PharmaceuticalPages ............................... 9
General InterestPages ............................. 13
PRODUCT INFO:HPLC/ICPages .................... 3-11, 16
GCPages ........................ 12-14
Sample HandlingPages ........................ 14-15
2
INDUSTRY FOCUSPharmaceuticalSeparation of a Dissimilar Complex Antibiotic Pair Using
a New Reversed-Phase HPLC Column and ELSD 9
General InterestOnsite Generation of Fuel Gases for Flame
Ionization GC Detectors 13
HPLC/ICNew! Alltima™ HP HPLC ColumnsHigh performance from high purity silica 3
Alltima HP HPLC Column Applications 4
ProSphere™ HPLC ColumnsHPLC columns for the life science industry 5
Astec CHIROBIOTIC™ HPLC ColumnsSimplify your most challenging chiral separations 6
Alltech's Method Development Assistant SoftwareMeet your new assistant 7
Alltech Degassing SystemsThe most advanced degassing technology 8
Model 2000 and Model 800 ELSDSensitive, universal HPLC detection 8
Ion Chromatography Starter KitsSave money with starter kits 10
New! Ion SuppressorsOutlast and outperform membrane suppressors 11
SofGrip™ HPLC FittingsThe toughest fitting with the softest grip 16
GCNew! Parker Balston® Model FID-1000 Gas StationHydrogen and zero air from one gas generator 12
Alltech Indicating Hydrocarbon TrapSee for yourself - the only indicating hydrocarbon traps 12
Alltech Digital Flow Check™ Mass FlowmetersA better way to measure gas flow 14
SAMPLE HANDLINGDrug-Clean™ SPE ColumnsGet more accurate drug analysis results 14
Autosampler VialsTake advantage of our special pricing 15
In This Issue:
3www.dongmyung.co.kr
New! High performance from high purity silica
Degassing Systems SpecificationsPhase Particle Size Carbon Loading Pore Size Surface Area End Capped Position
C18 3 and 5µm 12% 190Å 200m2/g Yes Most popular phase
C8 3 and 5µm 8% 190Å 200m2/g Yes Faster peak elution
C18 HiLoad 3 and 5µm 24% 100Å 450m2/g Yes More retentive for diffi cult separations
EPS C18 3 and 5µm 4% 190Å 200m2/g No Unique polar selectivity
C18 Amide 3 and 5µm 12% 190Å 200m2/g Yes Low bleed MS applications: 100% aqueous
compatibility
Cyano 3 and 5µm 4% 190Å 200m2/g Yes Unique retention and stability
Silica 3 and 5µm - 100Å 450m2/g No Normal phase
AlltimaTM HP HPLC ColumnsC18 C18 EPS
PARTICLE C18 C8 HiLoad Amide C18 Cyano SilicaSIZE LENGTH X ID PART NO. PART NO. PART NO. PART NO. PART NO. PART NO. PART NO.
Analytical 3µm 100 x 4.6mm 87667 87739 87685 87721 87703 87757 877853µm 100 x 2.1mm 87669 87741 87687 87723 87705 87759 87787
3µm 150 x 4.6mm 87668 87740 87686 87722 87704 87758 87786
3µm 150 x 2.1mm 87670 87742 87688 87724 87706 87760 87788
5µm 150 x 4.6mm 87679 87751 87697 87733 87715 87769 87797
5µm 150 x 2.1mm 87681 87753 87699 87735 87717 87781 87799
5µm 250 x 4.6mm 87680 87752 87698 87734 87716 87780 87798
5µm 250 x 2.1mm 87682 87754 87700 87736 87718 87782 87802
Rocket™ 3µm 33 x 7mm 87671 87743 87689 87725 87707 87761 877893µm 53 x 7mm 87672 87744 87690 87726 87708 87762 87790
Expedite™ 3µm 10 x 2.1mm 87673 87745 87691 87727 87709 87763 877913µm 10 x 4.6mm 87675 87747 87693 87729 87711 87765 87793
3µm 20 x 2.1mm 87674 87746 87692 87728 87710 87764 87792
3µm 20 x 4.6mm 87676 87748 87694 87730 87712 87766 87794
4
Alltima HP Application
Peak Identification1. Methionine Enkephalin2. Leucine Enkephalin3. Angiotensin II4. Neurotensin5. Angiotensin I6. Substance P
Peptide Mix
Column: Alltima HP C18, 5µ, 250 x 4.6mm Mobile Phase: A: 0.1% TFA in water / B: 0.08% TFA in
acetonitrile Gradient: (Time,%B),(0,20),(20,40) Flow Rate: 1.0mL/min Detector: UV at 220nm
10369
Peak Identification1. Desipramine (0.05 mg/mL)2. Nortriptyline (0.05 mg/mL)3. Diphenhydramine (0.25 mg/mL)4. Amitriptyline (0.05 mg/mL)
Tricyclic Antidepressants
Column: Alltima HP C8, 5µ, 150 x 4.6mm Mobile Phase: A: 25mM Phosphate Buffer pH7 / B: Methanol Gradient: (Time,%B),(0,60),(8,75),(20,75) Flow Rate: 1.0mL/min Detector: UV at 254nm
10373
Peak Identification1. Sulfanilamide2. Sulfamerazine3. Sulfamethazine4. Sulfamethizole
Sulfa Drugs
Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (85:15) 0.1% phosphoric acid:acetonitrile Flow Rate: 1 mL/min Detector: UV at 254nm
10351
Peak Identification1. Ethotoin2. Phenytoin3. Mephenytoin4. Carbamazepine
Anticonvulsants
Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (70:30) 25mM ammonium acetate:acetonitrile Flow Rate: 1 mL/min Detector: ELSD
10349
Peak Identification1. Cytidine2. Uridine3. Xanthosine4. Guanosine5. Adenosine
Nucleosides
Column: Alltima HP EPS C18, 5µ, 150 x 4.6mm Mobile Phase: A: 0.03M potassium phosphate, pH3.2 /
B: acetonitrile Gradient: (Time,%B),(0,5),(2,5),(20,30) Flow Rate: 1 mL/min Detector: UV at 260nm
10347
Peak Identification1. Nortriptyline2. Diphenhydramine3. Amitriptyline
Basic Drugs
Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (85:15) methanol:25mM potassium phosphate pH 7 Flow Rate: 1mL/min Detector: UV at 254nm
10352
5www.dongmyung.co.kr
Alltech ProSphere™ HPLC Columns
Other column dimensions are available. Visit our web store at www.alltechWEB.com or call your local Alltech office.i
ProsphereTM HPLC ColumnsDESCRIPTION LENGTH X ID PART NO.
ProsphereTM C18 150 x 150µm 32904300Å 250 x 150µm 329055µm 150 x 300µm 32907
250 x 300µm 32908
150 x 2.1mm 35098
150 x 4.6mm 32969
250 x 4.6mm 32971
ProsphereTM C4 150 x 150µm 33904300Å 250 x 150µm 339055µm 150 x 300µm 33907
250 x 300µm 33908
150 x 2.1mm 35096
150 x 4.6mm 33989
250 x 4.6mm 33991
ProsphereTM C18 150 x 150µm 35504100Å 250 x 150µm 355055µm 150 x 300µm 35507
250 x 300µm 35508
150 x 2.1mm 35090
150 x 4.6mm 35091
250 x 4.6mm 35087
Request a FREE copy of our new ProSphereTM Life Science Catalog
Introducing a full line of Size Exclusion, Ion Exchange, Affinity, Hydrophobic Interaction,and Reversed-Phase Chromatography Columns. To receive a catalog, use the insert,request on-line at www.alltechWEB.com, or call 1-800-ALLTECH.
6
For additional information on chiral separations, visit our online technical library at www.alltechWEB.com.i
Separation of Metoprolol on CHIROBIOTICTM T
Separation of Bupivacaine on CHIROBIOTICTM V
Methanol with Ammonium Trifl uoroacetate at 0.1% by weight1.0mL/min
Methanol with Ammonium Trifl uoroacetate at 0.1% by weight1.0mL/min
Astec CHIROBIOTIC™ HPLC Columns
CHIROBIOTICTM V: amines; including cyclic and tertiary amines, esters, neutral molecules and profens
CHIROBIOTICTM T: -halogenated aliphatic acids, amino acids, beta-blockers, carboxylic acids, cyclic aromatics, neutral aromatics, and small peptides
CHIROBIOTICTM R: -hydroxy acids, anionic molecules, aromatic esters, alcohols, di- and tri-peptides, and substituted -halogenated acids
CHIROBIOTICTM TAG: amino acids, beta-amino acids, carnitine, courmarins, diazopines, hydantoins, oxazolidinones, phosphonates, small peptides, and sulfoxides
FREEChiral MethodDevelopment
Wall Chart with Purchase!
Use Promo Code SP035
Peak 1 - 15.4min.Peak 2 - 17.1min.
Peak 1 - 5.6min.Peak 2 - 6.2min.
Astec CHIROBIOTICTM HPLC ColumnsCHIROBIOTICTM V CHIROBIOTICTM T CHIROBIOTICTM R CHIROBIOTICTM TAG
LENGTH X ID PART NO. PART NO. PART NO. PART NO.
100 x 4.6mm 11022AC 12022AC 13022AC 14022AC
150 x 4.6mm 11023 12051 12516 14023AC
250 x 4.6mm 11024 12053 12518 14024AC
Guards Cartridge, 20 x 4.0mm 11019 12445 12522 14100AC
Holder 11014 11014 11014 11014
7www.dongmyung.co.kr
New! Alltech’s Method Development Assistant Software
Your Assistant Helps Develop New Methods
Column kit includes:
150 x 4.6mm, 5µm columns from our premium, and best selling product lines.- PrevailTM C18- PrevailTM Select C18- AlltimaTM HP EPS- AlltimaTM HP C18 HiLoad- AlltimaTM HP Amide
FREE Column Kit!
Your Assistant Gets Existing Methods to Work
Meet Your New Assistant . . .
Method Development ToolsDESCRIPTION PART NO. PRICE
Method Development ToolsAnalytical Column Kit 22345 2500
Software Alone 22348 3118.75
Software and FREE Column Kit 22347 3118.75
For more information on Alltech's MDATM, visit our online technicallibrary or call for a demo or quote.i
8
Degassing Systems and ELSDsLC Instruments to Address Specific Needs and Budgets
The most advanced degassing technology
Alltech On-line Degassing Systems offer:
Sensitive, universal HPLC detection
Model 2000 ELSD Model 800 ELSD
For information on the ELSDs, visit our online technical library, or call and request brochures 474 and 475.For information on the Degassing Systems, visit our online technical library, or call and request brochure 479.i
Separation of a Dissimilar Complex Antibiotic Pair Using a New Highly Versatile Reversed-Phase HPLC Column and ELSDRomulus Gaita and Dr. Cary Thrall, [email protected]
Industry Focus - HPLCpharmaceutical
9www.dongmyung.co.kr
See page 8 for ELSD information. For information on PrevailTM Select C18, visit our online technical library at www.alltechWEB.com, or call and request document M076.i
1. Tylosin2. Monensin
Figure 2
Column: PrevailTM Select C18, 5µm, 150 x 4.6mm (Part No. 99300)
Mobile Phase: 95:5 Acetonitrile:Wateradjusted to pH 9 w/NH4OH
Flowrate: 1.0mL/minDetector: ELSD 2000 N2 Flow: 2.5L/min Drift Tube: 75°C, Impactor Off
Figure 1a - Monensin
Figure 1b - Tylosin
Figure 3
10
Ion Chromatography Starter Kits
U.S. EPA Methods Anion Column Kit - for EPA Methods 300.0, 300.1, 317.0. and 326.0
Includes a NovosepTM A-2 Column (250 x 4.0mm), guard cartridges, a 3.6mM carbonate EZ-LUTETM Concentrate, and anion and oxyhalide standards.
Suppressor-Based Anion Column Kit - for analysis of anions by suppressor-based methods
Includes a NovosepTM A-1 Column (150 x 4.6mm), guard cartridges, a 1.7mM/1.8mM bicarbonate/carbonate EZ-LUTETM Concentrate, and anion standards.
Non-Suppressed Anion Column Kit - for analysis of anions by non-suppressed methodsIncludes an AllsepTM Anion Column (100 x 4.6mm), guard cartridges, a 4mM pHBA EZ-LUTETM Concentrate, and anion standards.
Mono- and Divalent Cation Column Kit - for simultaneous analysis of mono- and divalent cations
Includes a Universal Cation Column (100 x 4.6mm), guard cartridges, a 3mM methanesulfonic acid EZ-LUTETM Concentrate, and cation standards.
IC Accessory Kit - tools and accessories for every IC systemIncludes PEEK tubing, one-piece fi ttings, a PEEK union, two different size plastic syringes, a luer-hub needle, tubing cutter, wrench, and two different in-line solvent fi lters.
Request a FREE copy of our new Ion Chromatography brochure
Alltech’s new Ion Chromatography Brochure includes all the products you need toperform high-quality ion chromatography separations. Learn about our new UniversalCation HR Columns and our new low-cost, high-sensitivity suppressors. Browse ourcomplete line of IC standards, including new standards for amines and organic acids.Choose Alltech for your IC needs, and receive the friendly, helpful customer support youexpect and deserve. To receive a brochure, use the insert, request on-line atwww.alltechWEB.com, or call 1-800-ALLTECH .
IC Starter KitsDESCRIPTION PART NO.
U.S. EPA Methods Anion Column Kit 270107
Suppressor-Based Anion Column Kit 270106
Non-Suppressed Anion Column Kit 270102
Mono- and Divalent Cation Column Kit 269105
IC Accessory Kit 269101
Kit includes a collection of tubing, fi ttings, and other
hardware.
Kits include an analyticalcolumn, guard columns,buffer concentrate, and
standards.
Accessory Kit Column Kits
11www.dongmyung.co.kr
New! IC Suppressors
Alltech SuppressorsDESCRIPTION CAPACITY PART NO.
Model 640 Suppressor (Isocratic)120V 20mN Sodium 640100
240V 20mN Sodium 640200
Model 641 Suppressor (High Sensitivity and Gradient)120V 40mN Sodium 641100
240V 40mN Sodium 641200
For information on the Alltech Suppressors, visit ouronline technical library at www.alltechWEB.com, orcall and request brochure 484.i
1. Methionine Enkephalin2. Leucine Enkephalin
1. Methionine Enkephalin2. Leucine Enkephalin
Peptide Mix
Peptide Mix
Peptide Mix
Column: Alltima HP C18, 5µ, 250 x 4.6mm Mobile Phase: A: 0.1% TFA in water / B: 0.08% TFA in
acetonitrile Gradient: (Time,%B),(0,20),(20,40) Flow Rate: 1.0mL/min Detector: UV at 220nm
9290
9291
Only Alltech’s suppressors degas the effl uent for high sensitivity,stable baselines, and better resolving power.
* U.S. patent numbers 6,444,475; 6,468,804
12
AT™-Indicating Hydrocarbon Traps AT™-Indicating Hydrocarbon In-Line Trap
AT™-Indicating HydrocarbonCartridge Trap
AT™-Indicating Hydrocarbon TrapsDESCRIPTION PART NO.
In-Line Trap, 1/8" fittings 7210
In-Line Trap, 1/4" fittings 7212
Cartridge Trap (requires baseplate) 81017
Cartridge Baseplate 81025
New! Parker Balston Model FID-1000 Gas Station
Model FID-1000 Gas Station SpecificationsGAS PRESSURE PURITY FLOW
Hydrogen: 0-90psig 99.9995% 90cc/min
Zero Air: 0-60psig
13www.dongmyung.co.kr
Onsite Generation of Fuel Gases for Flame Ionization GC DetectorsReggie Bartram, [email protected]
Abstract
Introduction
Conclusion
References1. Compressed Gas Association, Inc. Handbook
of compressed gases, New York: Van Nostrand Reinhold Co., 1981
2. Robert Daly, The Shift to On-Site Gas Generation, American EnvironmentalLaboratory, March 1995
3. Air Products and Chemicals, Inc. Safety-gram-14. Don’t turn a cylinder into a rocket: 1988.
4. Albert A, et al. What happens to gas cylinder purity as content is consumed? IEEE/SEMI Advanced Semiconductor Manufacturing Conference, 1993, Boston, MA
Industry Focus - GC
Baseline Comparison
general interest
14
Alltech Digital Flow Check™ Mass Flowmeters
New! Mixed-Mode Drug-Clean™ SPE ColumnsGet more accurate drug analysisresults
Model DFC-HRTM
Digital Flow CheckTM Flowmeters Specifi cationsFlow range: DFCTM 0.1-500 SCCM (except CO2 to 300 SCCM)
DFC-HRTM 0.01-500 SCCM (except CO2 to 300 SCCM)
Accuracy: +/-2% of reading Display: 16-character alphanumeric LCD, autoranging
Display modes: mass fl ow, split ratio, linear velocity (std. fl ow range)
Power: 9V alkaline battery (included), auto-off function,
low battery warning
Dimensions: 190.5 x 57.4 x 101.6mm (7.5" x 2.26" x 4.0")
For a complete list of drug standards,visit our online technical library atwww.alltechWEB.com.i
Digital Flow CheckTM FlowmetersMODEL PART NO.
DFCTM 4700
DFC-HRTM 5700
Mixed Mode Drug-CleanTM ColumnsDESCRIPTION QTY PART NO.
200mg/4mL 50 207010
Cocaine and Benzoylecgonine Extract from Urine
1. Cocaine (2.5 mg/ml) 2. Benzoylecgonine-TMS,
Benzoylecgonine-d3-TMS (5.0mg/mL)
Column: Helifl ex® AT-5ms, 30m x 0.25mm x 0.25µm (Part No. 15807)
Temp: 225°C (0 min hold) to 315°C (11 min hold) at 10°C/min
Carrier: Helium at 0.8mL/min (28cm/sec)
Detector: MSD at 325°C
2656
15www.dongmyung.co.kr
Alltech Autosampler Vials
Convenience Kits Ordering InformationLINER DESCRIPTION TFE/BUTYL LINER TFE/SILICONE LINER TFE/SILICONE/TFE LINER
VIAL AND CLOSURE DESCRIPTION KIT PART NO. KIT PART NO. KIT PART NO.
12x32mm Crimp Style Vials, 11mm SealsStandard Mouth Crimp Vials, Aluminum Seals 95161 98318 98373
WIDE Mouth Crimp Vials, Aluminum Seals 98397 98419 98447
Snap Seal Vials, Clear Poly CrimpTM Seals 88487 88496 88494
12x32mm Clear Screw Thread VialsStandard Mouth Vials, 8/425 Black Cap 98380* 89074 89078
WIDE Mouth Vials, 10/425 Black Cap 97243 97239 97252
Robotic R.A.M.TM‚ Vials, 9mm Black Cap 89135 89145 89149
* Preassembled kits only
For more information on vials, visit our online technical library at www.alltechWEB.com, or call and request brochure 445.i
Screw Thread Vials and Caps
Kits contain: Unassembled, 100 each Clear Glass Vials, Caps with Liners
Crimp Vials with Aluminum Seals Snap Seal Vials with Poly CrimpTM Seals
Q:
A:
SofGrip™ HPLC Fittings
www.alltechWEB.com
01/0
4 #
489K
© Copyright 2003 Alltech Associates, Inc.Alltech reserves the right to change prices and/or specifications without prior notification.
Use of Our Products: Alltech Associates, Inc., supplies chromatographic equipment reagents and accessories for non-medical
laboratory use only. This means our products: ... may NOT be used as drugs, ...may NOT be used as cosmetics, ...may NOT be
used as medical devices or as in vitro diagnostic devices.
Printed in the USA.
SofGripTM One-Piece FittingsSTANDARD NO-SLIPTM
COLOR QTY PART NO. PART NO.
Black, Short 10 40501 40511
Black, Long 10 40506 40516
Red, Short 10 40502 40512
Blue, Short 10 40503 40513
Green, Short 10 40504 40514
Yellow, Short 10 40505 40515
Assortment, Short 10 40507 40517
OtherFittings
No-SlipTM
Fitting
The trademarks referred to herein are the property of their respective owners.