Selecting a LIMS for the Next-Generation Genomics Lab

Selecting a LIMS for the Next-Generation

Genomics Lab

Bruce Pharr, VP Products & MarketingDr. Michael Kuzyk, Senior Product Manager, Genomics

BioIT World Conference & Expo, Boston, MA April 13th, 2011

Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.

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To sequence a bacterium genome in:

199513 months$0.50/base pair

2009less than a daypennies/base pair

Data Volume

Time & Money

NGS

Source: Garrity, GM, et al. Studies on Monitoring and Tracking Genetic Resources. Report from Convention on Biological Diversity, 2-8 April, 2009, Paris.


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63% of NGS laboratory directors surveyed said that data storage, data management and informatics are the biggest hurdles to expanding next-generation sequencing.

Data Overload

Source: Next Generation Sequencing SurveyJ.P. Morgan Equity Research, May 12, 2010

Selecting a LIMS for Next-Generation Genomics Labs

EXPLOSIVE Next-Generation Sequencing Instrument Growth

© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.

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Source: Caliper Life Sciences Q3 2010 Earnings Presentation (public filings and industry estimates)

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What is a LIMS?Laboratory Information Management System

• Commercial software available since 1980s

• Proven technology in multiple industries

• Special requirements for next-generation genomics research


Most genomics labs installing an NGS instrument will already have one or more of the following instrument types:

A Simplified Next-Generation Genomics Laboratory Information Flow

• Low-throughput sequencer• Microarray• qPCR

Instrument Interface

Upstream Applications

Downstream Applications & Analysis

User Interface

LIMSSoftware

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Selecting a LIMS for Next-Generation Genomics Labs7

Does Your LIMS Enable You to Get Your NGSInstruments Up and Running Quickly?

Project Initiation &

Sample Submission

Sample Prep/ Workflow Cluster

GenerationLibrary Prep/

Workflow Sequencing

Downstream Analysis

Data Pipeline

Reports/ ResultsSharing

Sample QC

Rework Pass

mRNA Purification, Fragmentation & cDNA Synthesis

Repair Ends

Adenylate ends & Ligate Adapters

ValidateLibrary

Rework

cBot Cluster StationHiSeq 2000/1000,

HiScanSQ, GAII/GAIIx

LibraryNormalization

Library Pooling(optional)

Cluster Generation

IlluminaSequencing

Initiate Analysis(optional)

Pass



Can you automate sample tracking, add new analysis methods or optimize laboratory workflows?


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How Easy is the LIMS to Configure and Customize?

LIMS Software ExternalApplications


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Does the LIMS Accommodate Different Users and Workflows?

Centralized Sample Dashboard

•Quickly locate the samples you are working on•A simple way to record completed work•The LIMS becomes a tool, not a chore


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Does the LIMS Accommodate the Special Requirements of Next-Gen Sequencing?

Sample Queues

•Display necessary sample information at each step•Alter information displayed at each step


1. Does Your LIMS Enable You to Get Your NGS Instruments Up and Running Quickly?

2. How Easy is the LIMS to Configure and Customize?

3. Does the LIMS Accommodate Different Users and Workflows?


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Three Criteria to Consider When Evaluating a LIMS for Genomics

DOWNLOAD this White Paper at:www.genologics.com/SelectingGenomicsLIMS

Selecting A LIMS for the Next-Generation Genomics Lab

Bruce Pharr, VP Products & MarketingDr. Michael Kuzyk, Senior Product Manager, Genomics

BioIT World Conference & Expo, Boston, MA April 13th, 2011

Documents

Selecting a LIMS for the Next-Generation Genomics Lab