SCHOTT

SCHOTT NEXTERION MTP-96Maximum & Minimum filling volumes per well

SCHOTT TGS PRODUCT GROUP NEXTERION

© SCHOTT Technical Glass Solutions GmbH

Corinna Schwung, 04/18/23

Experimental Details

Substrate tested:

- MTP 2-2259 without patterning 18.01.11

Buffer Solutions tested:

- PBS (1x) with 0.08 mg/ml Dye 5

- PBST 0.1% Tween with 0.08 mg/ml Dye 5

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Filling Scheme

1 2 3 4 5 6 7 8 9 10 11 12

A 130 140 150 160 170 180 190 200 210 220 230 240

B 130 140 150 160 170 180 190 200 210 220 230 240

C 10 20 30 40 50 60 70 80 90 100 110 120

D 10 20 30 40 50 60 70 80 90 100 110 120

E 10 20 30 40 50 60 70 80 90 100 110 120

F 10 20 30 40 50 60 70 80 90 100 110 120

G 130 140 150 160 170 180 190 200 210 220 230 240

H 130 140 150 160 170 180 190 200 210 220 230 240

  PBST

Volume in µl

  PBS

These wells were filled up to maximum liquid level.

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Results from adding 10 – 120 µl per well

1 2 3 4 5 6 7 8 9 10 11 12

A

B

C

D

E

F

G

H

- Solution pipetted into upper left corner

- MTP was gently shaken

Minimum Volumes

- PBST: 40 µl

- PBS: 50 µl

PBST

PBS

10 µl 120 µl

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Results from adding 10 – 240 µl per well

1 2 3 4 5 6 7 8 9 10 11 12

A

B

C

D

E

F

G

H

PBST

PBS

10 µl 120 µl

130 µl 240 µl

PBST

PBS

130 µl 240 µl

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Results from adding 10 – 240 µl per well13

0 µl

240

µl

PBS

PBST14

0 µl

150

µl

160

µl

170

µl

180

µl

190

µl

200

µl

210

µl

220

µl

230

µl

PBS

PBST

130

µl

240

µl

140

µl

150

µl

160

µl

170

µl

180

µl

190

µl

200

µl

210

µl

220

µl

230

µl

- Safe filling volume is about 160 µl for PBS, 130 µl for PBST

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Results from adding 10 – 240 µl per well

PBS

PBST

- The maximum filling level was not reached with these volumes

240

µl

190

µl

200

µl

210

µl

220

µl

230

µl

240

µl

190

µl

200

µl

210

µl

220

µl

230

µl

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Results from adding 320 – 400 µl per well

PBS

PBST

400

µl

370

µl

380

µl

390

µl

320

µl

350

µl

340

µl

330

µl

Maximum Volume per well

- PBS: 410 µl

- PBST: 340 µl

SCHOTT TGS PRODUCT GROUP NEXTERION

Corinna Schwung, 04/18/23

© SCHOTT Technical Glass Solutions GmbH

Conclusions

The minimum volume that will cover the well bottom is: 40 microlitres (with detergent) 50 microlitres (without detergent)

The maximum recommended „safe“ working volume is: 130 microlitres (with detergent) 160 microlitres (without detergent)

SCHOTT

SCHOTT NEXTERION MTP-96Maximum & Minimum filling volumes per well