Saturday Study Session 2Theme of the day:

Information TransferSession 3 – Modern Genetics

Question 1 Answer

BClue:3’5’

Semi Conservative process of DNA Replication

The parent molecule has two complementary strands of DNA. Each base is paired by hydrogen bonding with its specific partner, A with T and G with C.

The first step in replication is separation of the two DNA strands.

Each parental strand now serves as a template that determines the order of nucleotides along a new, complementary strand.

Replication Bubble

5 Carbon Sugar Important Parts(It could be DNA or RNA)

Question 2 Answer

AClue: recognized

Nucleotide Excision Repair

Question 3 Answer

C

Polymerase Chain Reaction (PCR)

Question 4 Answer

DClue: 5’ vs 3’

Complimentary strands

5’ – AUGAAAUCCUAG – 3’ mRNA molecule3’ – TACTTTAGGATC- 5’ DNA template molecule

Question 5 Answer

AClue: only one ≠ poly…

As in polyribosomes

Polyribosomes

Amino Acid Codon Chart

Ribosome Structure

Question 6 Answer

BClue: lactose binds to the

operator

Repressor

DNA lacl

Regulatorygene

mRNA

5

3

RNApolymerase

ProteinActiverepressor

NoRNAmade

lacZ

Promoter

Operator

Lactose absent, repressor active, operon off

Operon and Inducers

DNA lacl

mRNA5

3

lac operon

Lactose present, repressor inactive, operon on

lacZ lacY lacA

RNApolymerase

mRNA 5

Protein

Allolactose(inducer)

Inactiverepressor

-Galactosidase Permease Transacetylase

Operon Regulation

Promoter Promoter

DNA trpR

Regulatorygene

RNApolymerase

mRNA

3

5

Protein Inactiverepressor

Tryptophan absent, repressor inactive, operon on

mRNA 5

trpE trpD trpC trpB trpA

OperatorStart codonStop codon

trp operon

Genes of operon

E

Polypeptides that make upenzymes for tryptophan synthesis

D C B A

Operon Repressor

DNA

Protein

Tryptophan(corepressor)

Tryptophan present, repressor active, operon off

mRNA

Activerepressor

No RNA made

Math Grid In Answer

• The correct answer is: around 5,500 bp Acceptable range is: 5,300 – 5, 800 bp

• HindIII RFLPS = 4,500 and 1,000 = 5,500 bp• EcoRI = 5,500 bp (This plasmid only cut once; so the DNA strand is one linear piece.)Hind + Eco = 4,500 + 800 + 200 = 5,500 bp

Short Free Response 1(4 points possible)

• Possible points awarded for:• Discussion of point mutations being one nucleotide

altered in the DNA sequence.(1pt.)This will cause a possible change in the Amino Acid coded for in the protein. (1 pt.)

• Discussion of reading frame mutations being an addition or deletion of a nucleotide(s)to the existing DNA sequence. (1 pt.) This will cause all the reading frame codons to be altered down strand from the insertion/deletion.(1 pt.)

Point Mutation(A single point is changed)

Reading Frame Mutations(Everything behind is changed)

Short Free Response 2(4 points possible)

Possible points awarded for:Discussion of using the same restriction enzyme on both the plasmid and the human DNA source.(1 pt.)Discussion of the restriction enzyme creating matching sticky ends on each DNA strand. (1pt.)Discussion of the DNA pieces being combined using ligase to solidify the connects. (1 pt.)Discussion of the recombined plasmid being inserted back into the bacteria. (1pt.)

Build and reintroduce the Plasmid