bbemoldaenke

biological • biophysical • engineering

A powerful instrument for water toxicity assessment

continuous visual analysis of daphnia behaviour

w w w . b b e - m o l d a e n k e . d e

D a p h n i a T o x i m e t e r

The bbe Daphnia Toximeter observes daphnia online

D a p h n i a To x i m e t e r b b e m o l d a e n k e ©

Daphnia are established test organisms to indicate water quality. The bbe Daphnia Toximeter observes

daphnia under the influence of a constantly running sample water. bbe developed the 24/7

sensitive method to detect hazardous compounds in water from rivers (source-water protection),

plants, distribution systems and production drains to preserve human health and to monitor water. The

instrument is also designed as an early warning system to rapidly detect the entire range of dissolved

toxic compounds including pesticides, neurotoxins and warfare agents. Thus it is well suited to detect

wilful (terrorist attacks) or negligent incidents (spills, accidents). The bbe Daphnia Toximeter can also

be used for long-term monitoring for the “strategic” evaluation of water quality and as a valuable tool

in hazard management. The bbe Daphnia Toximeter has been deployed worldwide now for over 10 years.

Measurements

The method of image analysis enables a series of measurement methods and plausibility tests to assess thedaphnia behaviour using different criteria.

Speed measurements:D average speedD speed distribution

Behaviour observation:D swimming heightD fractal dimension - measurements for

turns and circling movements, curviness

Growth observation:D determination of daphnia size

Location:D no. of daphniaD distribution in chamber

Fractal dimensions: curviness and angle

Distance and grouping

Swimming height Daphnia growth

Shading: time index and distribution

Mobility and Agility of daphnia

Speed: average velocity and distribution

α

D = 1.09

D a p h n i a T o x i m e t e r i n a m e a s u r i n g s t a t i o n

Toxicity Index

The concept of the toxicity index is basedon the evaluation of certain measurands,such as swim speed or height, and chan-ges in these measurands. Only if more than 2 of the measurands simultaneously show unusual resultswithin a fixed period of time does theDaphnia Toximeter trigger an alarm. Dueto a dynamic alarm threshold an increasein daphnia size (growth) does not affectthe alarm.

Get to know your waters by rapid in situ profiles

G r a p h i c d i s p l a y f o r d a t a e v a l u a t i o n

W e i g h t i n g o f t h e t o x i c a l a r m

Operation

Sample water (0.5-2 l/h) continuouslyruns through the measuring chambercontaining the daphnia. The live imagesobtained using a CCD-camera are evalu-ated online with an integrated PC inorder to analyse changes in behaviour. Ifthis change is statistically significant, analarm is triggered.Depending on the physical properties ofthe water flow, the incoming water maybe subject to sample preparation. Thebbe WaterConditioner filters the sampleto remove suspended matters, heats thesample to an appropriate temperature toremove dissolved air, and has a high-pressure “back flush” option for samplewater with high turbidity.Remote access software enables directcontrol of the Daphnia Toximeter via remote PC and telephone lines. Readout,operational check and parameter adjust-ment can be performed by the user or bybbe as part of our support service.

D a p h n i a To x i m e t e r b b e m o l d a e n k e ©

D online display in LAND calibration of the instrumentD parameterisation of the

measurementsD data export to Excel or text filesD print function

Software

The bbe alarm software is used to record and analyse the data. The most important features are…

L i v e p i c t u r e a n d t r a c e i m a g e s c r e e n

D determination of different behavioural patternsD alarm analysisD saving of data and parametersD graphic display of all

measurement values

Your local bbe dealer…

D a p h n i a T o x i m e t e r

Specifications

Measurement procedure video image analysis

Housing material varnished steel plate

Weight 60 kg*

Dimensions (H x W x D) 620 x 620 x 600 mm*

Protection class IP54

Mains supply 110/240 V 50/60 Hz

Power consumption 600 W

Sample temperature 0 - 30 °C

Water requirement 60 l / h

Maintenance interval ≥ 7 days

Sample inflow free inflow/tube pump

PC system software OS Windows XP Prof.

Outputs modem, LAN, analog output 4-20 mA (2 X), relay output (2 X), RS232

bbemoldaenke

biological • biophysical • engineering

Wildrosenweg 3 | 24119 Kronshagen | Germany

phone (+49) 431 38040-0fax (+49) 431 38040 -10e-mail info@bbe-moldaenke.denet www.bbe-moldaenke.de

Subj

ect

to a

lter

atio

n!

* without WaterConditioner

Applications

D drinking water supply

D dam monitoring

D waterway analysis and assessment

D general environmental monitoring

D intake assessment

D toxicological evaluation

D limnological work

D research and education

D integrated PC with keyboard, touch pad and monitor

D WinXP, with graphic display, live pictures and intuitive user guidance and CD-RW

D alarm evaluation by the approved software provided by bbe Moldaenke

D elimination of disturbing air bubbles

D hardware diagnosis

D a p h n i a To x i m e t e r b b e m o l d a e n k e ©

T o x i m e t e r w i t h c o n d i t i o n e r u n i t

Features

D 1- or 2-chamber systems withup to 10 daphnia each

D integrated algae fermenter (constant supply) for daphnia feeding

D compact housing: IP54 security protection class

D separate compartment for flow-through cell and electricalcomponents