Role of Metalized Reflective Mulch with Nutritional and Insecticidal Treatments in Mitigation of HLB in

New Citrus Plantings Mini-Field Day, A Duda & Sons “Old Nursery” 19 June 2015

Phil Stansly, Scott Croxton & Joby Sherrod w/funding from CRDF

Table 1. Summary of cultural practices used for polyethylene mulch, insecticide, and foliar nutrition evaluation in citrus production with drip irrigation.

Field Summary Location Duda Grove LaBelle, FL Experimental Design

Split plot Randomized Complete Block Design: Main plots: (1) foliar nutrition alone, (2) insecticide alone, (3) both, (4) neither Subplots: with and without metalized mulch.

Irrigation 2, 2gal/hr BowSmith Drippers per tree + flood for freeze protection Tree Spacing 9 ft in row 23 between rows = 208 trees/acre Area 10 acres Planting Date July 3-4, 2012 Scion rootstock ‘Hamilin’orange on ‘Carrizo’ citromelo Rows North-South

Table 2. Insecticide applications from planting to present.

Insecticide Program

Date Product Active Ingredient Rate Method Areas Treated

July 9, 2012 Nuprid Imidacloprid 0.025 fl oz/tree Drench Insecticide Plots

October 11, 2012 Verimark Cyantraniliprole 0.1333 fl oz/tree Drench Insecticide Plots

October 112, 2012 Intrepid Methoxyfenozide 12 fl oz/ac Drench Entire Field

January 30, 2013 Platinum Thiamethoxam 0.0131 oz/tree Drench Insecticide Plots

March 7, 2013 Nuprid Imidacloprid 0.025 fl oz/tree Drench Insecticide Plots

March 7, 2013 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field

May 7, 2013 Verimark Cyantraniliprole 0.1333 fl oz/tree Drench Insecticide Plots

July 4, 2013 Agri-mek Abamectin 10 fl oz/ac Spray Entire Field

August 1, 2013 Nuprid Imidacloprid 0.05 fl oz/tree Drench Insecticide Plots

September 15, 2013 Closer Sulfloxaflor 5.25 fl oz/ac Spray Insecticide Plots

September 14, 2013 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field

October 8, 2013 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots

January 14, 2014 Danitol Fenpropathrim 16 fl oz/ac Spray Entire Field

February 3, 2014 Platinum Thiamethoxam 0.026 oz/tree Drench Insecticide Plots

March 5, 2014 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots

March 6, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Drench Entire Field

May 1, 2014 Nuprid Imidacloprid 0.1 fl oz/tree Drench Insecticide Plots

June 3, 2014 Agri-mek Abamectin 10 fl oz/ac Spray Entire Field

June 25, 2014 Verimark Cyantraniliprole 0.1333 fl oz/tree Drench Insecticide Plots

June 24, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field

July 30, 2014 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots

August 7, 2014 Agri-mek Abamectin 20 fl oz/ac Spray Entire Field

September 3, 2014 Movento Spirotetramat 16 fl oz/ac Spray Insecticide Plots

September 9, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field

October 15, 2014 Delegate Spinetoram 6 oz/ac Spray Insecticide Plots

January 8, 2015 Baythroid Beta-cyfluthrin 3.2 fl oz/ac Spray Entire Field

January 27, 2015 Portal Fenpyroximate 32 fl oz/ac Spray Insecticide Plots

March 24, 2015 AdmirePro Imidacloprid 14 fl oz/ac Drench Insecticide Plots

May 1, 2015 Apta Tolfenpyrad 27 fl oz/ac Spray Insecticide Plots

Table 3. Foliar nutrition applications from planting to present.

Foliar Nutrition Program

Date Product Rate/Acre

July 12, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal

August 7, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal

September 10, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal

October 2, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal

November 1, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal

February 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

March 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

April 2, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

May 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

June 3, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

July 3, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

August 1, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

September 10, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

October 8, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal

February 4, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal

March 5, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal

April 14, 2014 Soar Citrus Mix + N-Sure 1/2 gal + 1/2 gal

May 1, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal

June 4, 2014 Soar Citrus Mix + N-Sure 1/2 gal + 1/2 gal

July 9, 2014 Griffin Green 1 gal

August 5, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal

September 3, 2014 Griffin Green 1 gal

October 15, 2014 Tricite 20-20-20 10 lb

November 10, 2014 Tricite 20-20-20 10 lb

January 20, 2015 Tricite 20-20-20 10 lb

February 26, 2015 Tricite 20-20-20 10 lb

March 10, 2015 Fortress + N-Sure 1/2 lb + 1/2 gal

April 7, 2015 Griffin Green 1 gal

May 26, 2015 4-0-8 10 gal

Table 4. Mean (±SEM) canopy and soil temperatures recorded over a 24 month period every 20 min at center canopy and at 5.08 cm soil depth . Low 0.1%a High 0.1%b All Observations Hours over 380C

Mulch Canopy 2.16±0.28 43.21±0.12 23.11±0.21 445

No Mulch Canopy 5.68±0.18 40.15±0.11 22.63±0.33 327.33

Mulch Soil 9.78±0.07 38.36±0.06 25.16±0.26 66.67

No Mulch Soil 8.19±0.11 37.67±.05 24.1±0.34 41 aMean temperature for the lowest 0.1% of the recorded samples. bMean temperature for the highest 0.1% of the recorded samples.

Table 5. Yield and juice quality analysis of Fruit harvested 9 December 2014.

Total Yeild (lbs)

20 Fruit Weight (lbs)

20 Fruit Juice (lbs)

Acid Brix

Control 1977 8.78 3.025 0.375 8.125

Control+PRM 2585 8.3475 3.3375 0.3875 9.225

Foliar Nutrition 2076 7.7275 2.815 0.415 8.6125

Foliar Nutrition+PRM 3388 8.9775 3.655 0.368 9.2125

Insecticide 2736 9.3825 3.46 0.373 8.375

Insecticide+PRM 3217 8.7025 3.825 0.375 9.425

Insecticide+Foliar Nutrition

3168 9.015 3.335 0.375 8.275

Insecticide+Foliar Nutrition+PRM

2658 8.52 3.57 0.365 9.425

With Insecticide 11779 8.905 3.5475 0.3713 8.875

Without Insecticide 10026 8.458125 3.20813 0.3863 8.7938

With Foliar Nutrition 11290 8.56 3.3438 0.38 8.8813

Without Foliar Nutrition 10515 8.803125 3.41188 0.378 8.7875

With PRM 11848 8.636875 3.59688 0.3738 9.3219

Without PRM 9957 8.72625 3.15875 0.3838 8.3469

Figure 1. ACP sticky card captures recorded every two weeks from July 2012 to present.

Figure 2. ACP infested flush recorded every two weeks from July 2012 to present.

Figure 3. Trunk area measurements taken every 6 months from July 2012 to present.

Figure 4. HLB leaf samples taken every 6 months from July 2012 to present.