Rodent Surgery Course

Why????Laws and regulations dictate appropriate procedures and care

Laws and Regulations

You have a contract with the AICSurgical variables can affect animal welfare and research

You Have a Responsibility to the Animal

Success:Requires careful planning

Challenges.Same person is surgeon, anesthetist, scrub nurseExperimental design requires repetitive surgeriesSmall body size of rodents mandates use of microsurgery or ophthalmic instrumentsImmunodeficient rodents

Major Survival SurgeryAny surgical intervention that penetrates and exposes a body cavity or any procedure which produces permanent impairment of physical or physiologic functions

Minor Survival SurgerySurgery that does not expose a body cavity and causes little or no physical impairment

Non rodents: Dedicated surgical facility

Rodents: Surgical facility not required

Rodent Surgery AreaRoom or part of a room that is easily sanitized and not used for other activities when rodent surgery is in progress

Rodent Surgery AreaSubdivided into:Place for cages of rodents awaiting surgeryPrep areaSurgery areaRecovery area

Anesthesia

InhalationInjectable

InstrumentsQualityAppropriate size for procedureCorrect utilization

Surgical PackOrderSterilization indicators

Wound Closure

Surgical Needles

Surgical Adhesive

Preoperative ManagementAnimal health statusPeriod of stabilizationProper handling and restraint Stress-reductionFood and water NOT withheldAVOID antimicrobial agents

Perioperative ManagementPrepare surgery areaorganizedisinfect

Sterilization of InstrumentsAutoclave

Indications of Sterilization

Glass Bead SterilizerProvides rapid sterilization

ChemicalTime lengthyKill spores?????

Patient Prep

Prevent Hypothermia

Surgeon Prep

Operative TechniqueFollow aseptic surgery procedures

Utilize good surgical technique

Tunnel vascular catheters

Operative CareMonitoring the patient

Post-operative CareAnalgesiaHydration NutritionWound careSuture removal

Maintaining RecordsProcedure and datePost op careSuture removal

Monitoring Outcomes

PIs for donating extra animals for this lab!!