RNAi  in  rela+on  to  rice  development  under  salt    and  high  temperature  stress  

Neeti Sanan Mishra

Abiotic Stress

Approaches to combat abiotic stress

Change of tools……. RNA as novel regulators of gene expression

The power of miRNAs

Biogenesis n Action

miRNAs are encoded by MIR genes

Derived from a larger precursor, which is processed by RNaseIII-type enzyme, DCL 1

Potential to pair to mRNA and genomic sequences

N. Sanan-Mishra and S.K. Mukherjee The Open Plant Journal (2007)

Post-transcriptional gene regulation

Rice miRNAs on the Basis of Response to Heat and Salt

Dev

elop

men

t

Indica rice cultivar

Deep sequencing of miR populations

Inflorescence

Control conditions

Stress

Leaves

Roots Novel miRs

Known miRs

mirtrons Varieties

Expression analysis

Tissue-preferential expression patterns

Mittal et al., J.C.B. 2013

Comparative analysis

1200 rice miRs were expressed on microarray

75 miRs showed variation across NL(PB) and SL(PB) Out of these 75 miRs, 33 miRs, 51 miRs and 39 miRs were expressed in NL (PK), SL (PK) and SR(PK) respectively 10 miRs are expressed in response to both biotic and abiotic stresses 79 novel sequences validated

Profiling expression under stress

Osa-miRs & targets de-regulated by stress

GO annotation of the target pathways

Towards functional analysis

Case Study

(Okamura et al., Cell, 2007)

Search for new species: mirtrons

Rice mirtron validation

Joshi et al., Genomics 2012

Acknowledgements

Prof. S.K. Sopory Dr. S.K. Mukherjee, UDSC

Prof. A. Bhattacharya, JNU

Dr. D. Gupta, ICGEB

Dr. S. Mangurathia, DRR

Dr. D.N. Vinh, PPRI, Vietnam

Prof H. Khan, Univ Dhaka

Dr. S. Balasubramaniam, Monash Univ

Dr. N. Miiter, Univ Queensland

Dr. B. Carroll, Univ Queensland

Shikha Goel Sudhanshu Roy

Deepti Mittal Neha Sharma

Tran Quan Ngoc Abhishek Anand

Anita Dubey Kavita Goswami

Dept of Biotechnology (DBT) Dept of Science & Technology (DST)

ICGEB