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RNA Extrac+on: Trizol

RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

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Page 1: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA Extrac+on: Trizol

Page 2: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA Extrac+on: Trizol

Page 3: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA extrac+on from cells, +ssues, organs

Page 4: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Mammalian +ssues (DEPC treated)

Page 5: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA Extrac+on

Page 6: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA Analysis

OD260/OD280: 1.9-‐2.1 GOOD <1.8 Protein contamina+on >2.2 RNA degreda+on OD260/OD230: <2.0 Trizol contamina+on

Page 7: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA Electrophoresis

Page 8: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

mRNA prepara+on

Page 9: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Page 10: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Reverse Transcrip+on PCR

Page 11: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Hybridiza+on

Page 12: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Page 13: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Northern Blot

Page 14: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Northern Blot

Page 15: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA in situ hybridiza+on

Page 16: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA in situ hybridiza+on

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