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Rinse & Chill Technology
Novel Intervention for reduction of
undesirable bacteria on beef carcasses and On-going Protection from
coliforms and E. coli in vacuum packaged ground
beef
Rinse & Chill Technology
• Developed by MPSC, Inc. – St. Paul, MN
• Post bleed vascular technology
• Involves vascular rinse and chill of carcasses with a solution of saccharides, sodium chloride and phosphates
Rinse & Chill Technology
• Provides for statistically “cleaner” carcass samples with respect to aerobic microorganisms when compared with controls2 h cooler sponge samples/3 sampling dates
APC N Ave.CFU/cm2
% Reduction
P value(95% - .05)
Control 90 1224
R&C 90 731 40.3% .039*
Rinse & Chill Technology
• Provides for statistically “cleaner” carcass samples with respect to coliforms microorganisms when compared with controls
2 h cooler sponge samples/3 sampling dates
Coliforms N Ave.CFU/cm2
% Reduction
Frequency P value(95% - .05)
Control 90 97 22/90
R&C 90 0.7 99.3% 11/90 0.125
Control vs. Rinse & Chill
0.1
1
10
100
1000
10000
C T
APC
Coliforms
CFU/cm2
C = ControlT = R&C
Rinse & Chill Technology
• Provides for statistically “cleaner” carcass samples with respect to aerobics microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
APC N Ave.CFU/cm2
% Reduction
P value(95% - .05)
Control 50 580
R&C 50 341 41.2% .009*
Rinse & Chill Technology
• Provides for statistically “cleaner” carcass samples with respect to coliforms microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
Coliforms N Ave.CFU/cm2
% Reduction
Frequency P value(95% - .05)
Control 50 345 41/50
R&C 50 111 67.8% 31/50 0.002*
Rinse & Chill Technology
• Provides for statistically “cleaner” carcass samples with respect to generic E. coli microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
Generic
E. coli
N Ave.CFU/cm2
% Reduction
Frequency P value(95% - .05)
Control 50 294 31/50
R&C 50 48 83.7% 16/50 0.0008*
Control vs. Rinse & Chill
1
10
100
1000
C R&C
APC
Coliforms
G. E. coli
CFU/g
Rinse & Chill TechnologyOn-going Protection
• Significant on-going protection in further processing of carcasses, such as in ground beef
• Inoculated with E. coli 0157:H7; • Vacuum packaged; 40F; 2 trials; triplicate
Ave. (n=6)
Day 1Log10 CFU/g
Day 17Log10 CFU/g
Day 31Log10 CFU/g
Day 50Log10 CFU/g
Day 70Log10 CFU/g
Day 92Log10 CFU/g
C 4.65 3.81 3.06 2.68 2.77 2.27
R&C 4.76 3.39 2.05 1.81 2.07 0.82P value(95% - .05)
0.141 0.005* 0.007* 0.002* 0.0006* 0.021*
Vacuum-packaged ground beef; 40 FE. coli 0157:H7
Control vs. Rinse & Chill
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
0 20 40 60 80 100
C
R&C
Log10 CFU/g
Day
Rinse & Chill TechnologySummary
Carcass sponge
• Reduction in APC – 40% – 41.2%
• Reduction in coliforms– 99.3% – 67.8%
• Reduction in generic E. coli– 83.7%
Ground shoulder/chuck (50/50)
• Control – 2.38 log reduction in E. coli 0157:H7 in 91 days
• Rinse & Chill – 3.94 log reduction in E. coli 0157:H7 in 91 days
