RF Generator Power 　 1550W 　 　

Argon Gas Flow Rates

Plasma 15 L / Min

Carrier 0.8 L / Min

Makeup 0.35 L / Min

Collision Gas (He) 4 mL / Min

Spray Chamber Temp. 2 °C

Omega Lens 11.0 V

Octopole bias -18 V

Energy Discrimination 4.0 V

Replicates 3

Nebulizer Micromist Concentric

Isotopes monitored 　 55Mn, 56Fe, 59Co, 63Cu, 66Zn

Supple 1.

0 100 200 300 400 5000

5

10

15

20

25

30

Par

ticl

e num

ber

(%

)

Particle diameter (nm)

Al-R in Water Al-R in GA

Supple 2.

(Weeks)1 2 3 4 5 6 7 8

Bod

y w

eigh

t (g

)

0

525

30

35

40

45

50

Control1.5 mg/kg3 mg/kg6 mg/kg

Supple 3.

0 2 4 6 8 10 12 Necropsy (weeks)

Control 6 mg/kgKidney

Liver Control 6 mg/kg

Supple 4.

0 100 200 300 400 500

0

5

10

15

20

25

30

Par

ticl

e num

ber

(%

)

Particle diameter (nm)

Al-D in Water Al-D in GA Al-R in Water Al-R in GA

Supple 5.

Sample Solution Diameter (nm) Surface charge (mV)

Al-DIn Water 112.2 ± 31.8 53.78 ± 0.73

In GA 137.7 ± 40.2 39.55 ± 0.43

Al-RIn Water 107.5 ± 30.9 72.44 ± 7.25

In GA 92.9 ± 26.3 38.40 ± 1.35

(A) (B)

Supple 6.D

rink

ing

wat

er (

% o

f co

ntro

l, m

l/m

ice

b.w

.)

Die

t (%

of

cont

rol,

g/m

ice

b.w

.)

Al-S Al-R

LD

H in

Kid

ney

(%

of

con

trol

)

0

100

200500

510

520

530

540

Control5 ug/ml20 ug/ml

Al-S Al-R

LD

H in

Liv

er (

% o

f co

ntr

ol)

0

10

2080

100

Control5 ug/ml20 ug/ml

Al-S Al-R

LD

H in

Lu

ng

(% o

f co

ntr

ol)

0

2080

90

100

110

120

130

140

150

160

170

180

Control5 ug/ml20 ug/ml

Al-S Al-R

LD

H in

Hea

rt (

% o

f co

ntr

ol)

0

2080

90

100

110

120

Control5 ug/ml20 ug/ml

**

*

**

**

Supple 7.

Data for review only : Experimental design for consumption of diet and drinking water (n =3)

Cage No.1 Cage No.2 Cage No.3

Diet and drinking water Diet and drinking water Diet and drinking water

Data for review only : Experimental design using the blood

Hematology analysis

Biochemistry (n=4)

Cytokines (n=6)

Blood sampling (about 1.2 ml/mice)

500 μl

The rest: serum after centrifugation

Hematology analysis

500 μl

The rest: serum after centrifugation

200 μl 200 μl

Hematology (n=8)

The rest serum The rest serum

100 μl

Lysis

100 μl 100 μl 100 μl

ICP/MS analysis (n=3)

100 μl 100 μl 100 μl 100 μl 100 μl 100 μl 100 μl 100 μl

Data for review only : Experimental design for ICP/MS