Review on Nanoparticle Probes with Surface Enhanced

Raman Spectroscopic Tags (SERS Dots) for Cellular

Cancer Targeting

Dae Hong Jeong(Department of Chemistry Education, Seoul National University; jeongdhts'snu.ac.kr)

Abstract

This article is a review version of the article published on Oct. 2006 at

Analytical Chemistry, 78, 6967-6973. We have developed biocompatible,

photostable, and multiplexing-compatible surface-enhanced Raman

spectroscopic tagging material (SERS dots) composed of silver

nanopartic1e-embedded silica spheres and organic Raman labels for cellular

cancer targeting in living cells. SERS dots showed linear dependency of

Raman signatures on their different amounts, allowing their possibility for

the quantification of targets. In addition, the antibodyconjugated SERS dots

were successfully applied to the targeting of HER2 and CDIO on cellular

membranes and exhibited good specificity. SERS dots demonstrate the

potential for high-throughput screening of biomolecules using vibrational

information.

Key Words: SILVER NANOPARTICLES, SURFACE-ENHAl'\JCED

RAMAN SCATTERING, ANTIBOTY- ANTIGEN REACTION

Review on Nanoparticle Probes with Surface Enhanced

Raman Spectroscopic Tags (SERS Dots) for Cellular

Cancer Targeting

01 ~~ ~ 2006\i 10-%1. ~~:A1 Analytical Chemistrys] 78~ 6967-6973~O1l

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(b) MPTS7} ]i~01] ~lcj~ ~, (c) 2. g £~~ ~, (d) 2. 0] £~B ~.Q-] ~

rJ1B A}~.

-=:2 13 3. SERS dots.Q-] TEM A}~: (a) 4-MT7} ]i7:] B SERS dots,

(b) 2-NT7} ]iAl B SERS dots, (c) TP7} ]i7:l B SERS dots.

-=:213 4. ~cj51} ~g AJ}7] Zi.Q-] SERS dots~ SERS ~~E~:

(a)4-MT, 2-NT, TP Zf ]i~ SERS dots,

(b) TP2] *£01] u:j-~ A~E'iJ2] A-J]7] ~}0l.

-=:2 ttl 5. ~ cj 51} ~ g ~ ~~ SERS dots.Q-] SERS ~~ E ~:

(a) 4-MT, 2-NT, TP Zf ]i~ SERS dots,

(b) TP7} ]i7:] B SERS dot.Q-] OJoj] u:]-~ ~~ E ~.Q-] A-J] 7] ~}o]

-=:213 6. A-J].:£oJ]Al '5J~1l-'4 <i1~B SERS dot.Q..s. HER2 ~ CDIO ~A-J].:£ p}71

~ ~~~ ~JJl-:

(a) SERS dot4MT-HER2-'4 l:lR 0J~ MCF-7,

(b). SERS dotTP-CDlOJJl- l:lR 0J~ SP2/0,

(c) SERS dotTP-CDlOJ1} l:lRoJ~MCF-7,

(d) SERS dot4MT-HER2-'4 l:lRoJ~ SP2/0,

(e) SERS dot4MT-HER2-'4 l:lRoJ~ NHBE,

(f) SERS dotTP-CDlOJ1} l:IB 0J~ NHBE

Ag-embeddedsilica

OMe

Meo-rMe

HS

Ramanlabel

AgNO,NH40B

Ethyleneglycol

Rednction

\oO-ki~SH

?/

oHS~Si:_O

o\

MeOMeO-Si~SH

IOMe

Silica(180-210nm)

H,N5vol%APTS

NH,~,~-----

SERSDots

Sodiumsilicate

TEOS

l. Spacer

2MIPNS

3. Antibodies (Ber2,CDIO)

Targeting

Cell Membrane

:J..~ 2.

:J..~ 3.

400 600 800 1000 1200 1400 1600 1800

(b)

Raman Shift/ em-I

10nM

1nM

100 pM

400 600 800 1000 1200 1400

Raman Shift fernl

1600 1800

::L ~ 5.

(a)

4-MT T 10000counts

~

==~ 2-NT T8000 counts.....

;<;::'"=...-=-

T9000countsTP

400 600 800 1000 1200 1400 1600 1800

Raman Shift / em·t

.:-:/

o 5 10

SERSDot Concentration (mg/ml)

180

.~~ 900

..:

NNo

(J)(J)(J)

(b)

1000 1100-I

Raman Shift / em

.=:L~ 6

(a)

§.40

40

Length {f.tm

AS

FS

600 800 1000 1200 1400 1600 1800

Raman Shift I cm'

(b)

§.40

40

Length !1Ull

lsl/h/)l11600 900 1200 1500

Raman Shift I cm'

(c)

40

Length I urn

600 SOD 1200 \400 1600 1800

Raman Shift I ern"

(d)

(e)

3J 3S 40 45 Sl 55

I

\L.........-..--.. _

600

600

800 1000 1200 1400

Raman Shift I ern"

900 1200 1500

Raman Shift I cm'1800

203

m~ 20

Length I urn

ABC 0

,-~~~-----

---,-----.--,-~......---'r-·,..·~--·~--T-----..--·

1200 1~C'0 I·;C'~' 1:00

Raman Shift I cm '