Restriction Mapping

Central Dogma

Human Migration

Gel Electrophoresis

Restriction Enzymes: Cut at specific and unique sequences called Restriction Sites

Restriction Site MappingLike Solving a puzzle.

Boreal ToadsBufo boreas

boreas

Learning Goals

• Explain how Gel Electrophoresis works• Understand the difference between DNA,

RNA, mtDNA, nDNA• Make a restriction map from information on a

gel• Explain how a restriction map can be utilized

to explore evolution