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Reproduction of Surubim Reproduction of Surubim , Pacu and , Pacu and Arowana Arowana A A CRSP Project 200 CRSP Project 200 7-2008 7-2008 1 School of Environment and Natural Resources, The School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio, 43210, USA. Ohio State University, Columbus, Ohio, 43210, USA. 2 Nicolaus Copernicus University, Faculty of Biology Nicolaus Copernicus University, Faculty of Biology and Earth Sciences, and Earth Sciences, 87-100 Torun, 87-100 Torun, Poland Poland 3 Universidad Nacional Agraria de la Selva, Tingo Universidad Nacional Agraria de la Selva, Tingo Maria, Peru Maria, Peru 4 Sao Paulo State University, Aquaculture Center, Sao Paulo State University, Aquaculture Center, Jaboticabal, Brazil Jaboticabal, Brazil Konrad Dabrowski Konrad Dabrowski 1 , Marta , Marta Jaroszewska Jaroszewska 1,2 1,2 , Manuel Sandoval , Manuel Sandoval 3 , , Yongfang Zhang Yongfang Zhang 1 , , Bong Bong - - Joo Lee Joo Lee 1 , Kyle Ware , Kyle Ware 1 , Rodrigo , Rodrigo Takata Takata 4 Maria Celia Portella Maria Celia Portella 4 and and Wilfrido Contreras Wilfrido Contreras 5

Reproduction of Surubim, Pacu and Arowana ACRSP Project 2007-2008 1 School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio,

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Reproduction of SurubimReproduction of Surubim, Pacu and Arowana, Pacu and Arowana

AACRSP Project 200CRSP Project 2007-20087-2008

Reproduction of SurubimReproduction of Surubim, Pacu and Arowana, Pacu and Arowana

AACRSP Project 200CRSP Project 2007-20087-2008

11School of Environment and Natural Resources, The Ohio State University, School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio, 43210, USA.Columbus, Ohio, 43210, USA.

22Nicolaus Copernicus University, Faculty of Biology and Earth Sciences, Nicolaus Copernicus University, Faculty of Biology and Earth Sciences, 87-87-100 Torun, 100 Torun, PolandPoland

33Universidad Nacional Agraria de la Selva, Tingo Maria, Peru Universidad Nacional Agraria de la Selva, Tingo Maria, Peru 44Sao Paulo State University, Aquaculture Center, Jaboticabal, Brazil Sao Paulo State University, Aquaculture Center, Jaboticabal, Brazil 55University of Tabasco, Villahermosa, MexicoUniversity of Tabasco, Villahermosa, Mexico

Konrad DabrowskiKonrad Dabrowski11, Marta Jaroszewska, Marta Jaroszewska1,21,2 , , Manuel SandovalManuel Sandoval33 , Yongfang Zhang , Yongfang Zhang11, ,

BongBong--Joo LeeJoo Lee11, Kyle Ware, Kyle Ware11, Rodrigo Takata, Rodrigo Takata44 Maria Celia PortellaMaria Celia Portella44 and and Wilfrido ContrerasWilfrido Contreras55

Konrad DabrowskiKonrad Dabrowski11, Marta Jaroszewska, Marta Jaroszewska1,21,2 , , Manuel SandovalManuel Sandoval33 , Yongfang Zhang , Yongfang Zhang11, ,

BongBong--Joo LeeJoo Lee11, Kyle Ware, Kyle Ware11, Rodrigo Takata, Rodrigo Takata44 Maria Celia PortellaMaria Celia Portella44 and and Wilfrido ContrerasWilfrido Contreras55

Successful reproduction and rearing Successful reproduction and rearing of surubim in North Americaof surubim in North America

Successful reproduction and rearing Successful reproduction and rearing of surubim in North Americaof surubim in North America

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FD

A B

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FDD

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Embryonic development in Embryonic development in PseudoplatystomaPseudoplatystoma sp. sp.

Time after fertilization in hours (h) at 27Time after fertilization in hours (h) at 27ooC.C.

Black bars = 1 mm. (A) 1 h, 2-cells; (B) 2 h, 4-cells; (C) 4 h, ½ epiboly; (D) 6 h, blastopore closing; (E) 9 h, first somites; (F) 14 h, at hatching.

Surubim catfish Surubim catfish ((PseudoplatystomaPseudoplatystoma sp. sp.)) FebFebruaryruary 2007 2007

Columbus, OColumbus, Ohiohio

Yongfang ZhangYongfang Zhang

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Growth of pacu Growth of pacu ((Piaractus mesopotamicusPiaractus mesopotamicus) ) in in Columbus, Ohio.Columbus, Ohio. Environmental conditions control included: Environmental conditions control included: temperature, pH, temperature, pH, and light regime fluctuations. and light regime fluctuations.

Arrows indicate duration of water temperature cooling and short day light.Arrows indicate duration of water temperature cooling and short day light.

An immature necropsied pacu scanned An immature necropsied pacu scanned andand assessed via cross sections assessed via cross sections..

An Aloka (Aloka Co., LTD) SSD-500V ultrasound with a Medi-capture An Aloka (Aloka Co., LTD) SSD-500V ultrasound with a Medi-capture USB100 video capture system with an attached camera, and 3.5 MHz convex array probeUSB100 video capture system with an attached camera, and 3.5 MHz convex array probe..

AB = Air bladder; Bc = body cavity wall; F = fin; Fo = food; GB = gall bladder; In = intestine; AB = Air bladder; Bc = body cavity wall; F = fin; Fo = food; GB = gall bladder; In = intestine;

Ki = kidney; Li = liver; M = muscle; R = ribs; St = stomach; VF = visceral fat.Ki = kidney; Li = liver; M = muscle; R = ribs; St = stomach; VF = visceral fat.

BongBong--Joo LeeJoo Lee

Work in progress on reproduction of sWork in progress on reproduction of silver arowana ilver arowana ((Osteoglossum bicirOsteoglossum bicirrrhosumhosum))

JJuveniles 4 weeks after yolk sac absorptionuveniles 4 weeks after yolk sac absorption,,

February 24, 2007.February 24, 2007.

Adult, 16 months old,Adult, 16 months old,April 2008April 2008

Extension workshops on reproduction and rearing of tropical fishExtension workshops on reproduction and rearing of tropical fish in Manaus and Balbina, Brasil, May 2007in Manaus and Balbina, Brasil, May 2007

ManausManaus

BalbinaBalbina

Extention workshops on reproduction and rearing of tropical fishExtention workshops on reproduction and rearing of tropical fish in Tingo Maria and Lima, Peru, July 2007in Tingo Maria and Lima, Peru, July 2007

PaichePaiche((Arapaima gigasArapaima gigas))

Research publications

Arslan, M., K. Dabrowski, M.C. Portella and J. Rinchard. 2008. The role of dietary lipids and fatty acids in growth and body fatty acid composition of surubim (Pseudoplatystoma fasciatum). J. World Aquacult.Soc. 39: 51-61.

 Dabrowski, K., M. Arslan, J. Rinchard, M.E. Palacios. 2008. Growth, maturation, induced spawning,

and production of the first generation of South American catfish (Pseudoplatystoma sp.) in the North America. J. World Aquacult. Soc. 39: 174-183.

Portella, M.C., Dabrowski, K. 2008: Diets, physiology, biochemistry and digestive tract development of freshwater fish larvae. Science Publishers, Inc. (in press).

 Jaroszewska, M. and Dabrowki, K. 2008. Semionotiformes and Amiiformes. In: (Y.W. Kunz), Development of Non-teleost Fish, Science Publishers, Inc. (in press).

 Tratner , S., Pickova, J., Park, K.H., Rinchard, J. Dabrowski, K. 2007.

Effects of alfa-lipoic and ascorbic acid on the muscle and brain fatty acids and antioxidant profile of the South American pacu Piaractus mesopotamicus. Aquaculture 273: 158-164.

  Dabrowski, K., Zhang, Y., Arslan, M., Terjesen, B.F. 2007.

Indispensable amino acids deprivation does no cause rapid amino acid depletion in fish body and this principle has a potential to be used as the new strategy in nutrition. Revista Colombiana de Ciencias Pecurias 20: 508-511.