Element content of Fusarium-infected wheat genotypesSzeged – Timişoara axis for the safe food and feed

SZETISA1HURO/0901/147/2.2.2

Reporting period: 01.06. 2011 – 30.09. 2011

DisclaimerThe content of this page does not

necessarily represent the official position of the European Union.

2nd Progress Report MeetingSzeged, 22-23 September, 2011

Erdei László, Pető Andrea, Csiszár JolánDepartment of Plant Biology

University of Szeged

The wheat quality and consumer safety is threatened by Fusarium Head Blight (FHB) caused by i.e. Fusarium culmorum, F. graminearum and F. avenaceum. Diseased spikelets exhibit symptoms of premature bleaching shortly after infection.

Hypothesis: Fusarium infection influences physiological, biochemical and membrane-transport characteristics of the plant resulting in altered mineral composition of the grain.

Visible effects of Fusarium sp. on wheat

I. Gathering wheat ears and flag leaves 17. 06. 2010. Kecskéstelep,

Cereal Research Nonprofit Kht.1. GK Mini Manó/Nobeokabozu wheat population

(Prof. Mesterházy Ákos )

Genetic mapping population with 350 members.

The Fusarium resistance is a complex, quantitative character.The aim is to develop lines with several resistance genes. After crossing, the progenies will be inbred to increase the homogenity (recombinant inbred lines)

1. GK Mini Manó/Nobeokabozu genetic mapping population

(Prof. Mesterházy Ákos )

Nobeokabozu: FHB resistant lineA recombinant line from China: ‘WSY’, Wangshubai/Sumai3/Yangangfanzhou

GK Mini ManóArthur 71 / Sava // Rusalka / NS 171.2 /3/ F 30.74Resistant line to e.g. leaf rust

I. Gathering wheat ears and flag leaves 18. 05. 2011. Kecskéstelep,

Cereal Research Nonprofit Kht.1. GK Mini Manó/Nobeokabozu wheat population

(Prof. Mesterházy Ákos )

The aim is to develop lines with several Fusarium resistance genes. After crossing, the progenies will be inbred to increase the homogenity (recombinant inbred lines)

I. Sampling before inoculation18. 05. 2011.

• 3-3 ears with stem were taken for ROS and NO detections

The fluorescens investigations were done as soon as it was possible

• Determination of photosynthetic parameters on selected plants (Ferenc Horváth and Barnabás Wodala)

Timetable - Planned chronology

• 1rst measurements and sampling: 05.18.• Inoculation: 05. 26. with Fusarium culmorum (Fc12551) and

F. graminearum (Fg46.06 ) isolates• The bags were removed after 2 - 3 days• II. sampling after the symptomes will be developed

(ca. after 3 weeks)• III. seeds will be harvested after another 3 weeks

Lines inoculated with two Fusarium species:

Fusarium culmorumFusarium graminearum

Line 561 – very resistant against Fusarium (Sumai3 from China)

1/a – inoculated with Fusarium culmorum2/a – inoculated with Fusarium graminearum

Line 474 – earlier Fusarium resistant (alias 484), high Mn level in the ear

1/a – inoculated with Fusarium culmorum2/a – inoculated with Fusarium graminearum

Line 501– earlier Fusarium resistant (alias 515), low Mn level in the ear

1/a – inoculated with Fusarium culmorum2/a – inoculated with Fusarium graminearum

Line 483 – earlier Fusarium sensitive (alias 493), high Mn level in the ear

1/a – inoculated with Fusarium culmorum2/a – inoculated with Fusarium graminearum

Line 488 – earlier Fusarium sensitive (alias 500), low Mn level in the ear

1/a – inoculated with Fusarium culmorum2/a – inoculated with Fusarium graminearum

Accumulation of Cu, Mn, Fe, Zn mikroelements and K, Ca Mg macroelements

in different plant parts

Comparison of element concentrations in flag leaf, culm, green ear , and in the ripe ear in grains and husks

Wheat lines:

Sumai-3: resistant to Fusarium („line 561”)

Lines regarded as resistant: „474”, „501”

Lines regarded as sensitive: „483”, „488”

To be observed: gradients of element within the upper parts of the plant

Resistant Sensitive

High Mn

Low Mn

General gradient of Cu: from flag leaf to chaff

Resistant Sensitive

High Mn

Low Mn

General gradient of Mn: from flag leaf to chaff

ResistantSensitive

High Mn

Low Mn

General gradient of Fe from flag leaf to chaff

ResistantSensitive

High Mn

Low Mn

General gradient of Zn: accumulation in grain

Resistant Sensitive

High Mn

Low Mn

General gradient of K:accumulation in culm

Resistant Sensitive

High Mn

Low Mn

General gradient of Ca: accumulation in chaff

Resistant Sensitive

High Mn

Low Mn

General gradient of Mg: from flag leaf to chaff

Accumulation of Cu, Mn, Fe, Zn mikroelements and K, Ca Mg macroelements

in different plant parts:

Comparison of wheat lines and their responses to Fusarium inoculation

To be observed:

-direction of transport (e.g. from schaff to grain, Cu, Zn)

-Effects of inoculation

Conclusion

Gradient s of element concentrations are different from flag leaf to the grainusually decerasing (Cu, Mn, Fe)but increasing for Znonly xylem transport, Ca (it is known fact),

resulting in accumulation in the chaffaccumulation in the tissue of the culm (K).

Direction of transport (e.g. from schaff to grain, Cu, Zn)

Effects of inoculation, maybe artefact:

Thank you for your attention

and for the useful suggestions!!!