Replacing the Column Stabilizer Tube with Finger …...Reconnecting a Column Stabilizer to a New Column NOTE:You must disassemble the finger-tight fitting before reconnecting it to

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Replacing the Column Stabilizer Tubewith Finger-tight Fittings

This document explains how to replace the column stabilizer tube assembly with finger-tight fittings in the ACQUITY UltraPerformance LC® System column heater and column heater/cooler.a It includes instructions for:

• replacing the column stabilizer tube • reconnecting the column stabilizer tube to a new column• installing the column outlet fitting• installing and disassembling the finger-tight fitting

NOTE: Use this procedure when replacing a column stabilizer tube. If you are installing a new column stabilizer tube, column, or column in-line filter, refer to the “ACQUITY UPLC System Operator’s Guide” (PN 71500082502).

NOTE: For information on installing finger-tight reusable fittings on a column manager, see Waters procedure “Installing Finger-tight Fittings in the Column Manager,” available in the Waters Support Center (www.waters.com).

Parts RequiredThis procedure requires the column stabilizer kit for 50/100-mm columns or 150-mm columns (Table 1 and Figure 1). Use the part that corresponds to your column size.

NOTE: For spare parts related to finger-tight fittings, see page 2.

Figure 1 - Column Stabilizer Kits (Use the Part Corresponding to Column Size)

a. To be certain you have the latest version of this procedure, please visit the Support Center at Waters.com.

Table 1: Column Stabilizer Tube Assembly

Part Number Description Quantity

205000291 Column Stabilizer Kit, 50/100 mm 1

205000365 Column Stabilizer Kit, 150 mm 1

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REPLACING THE COLUMN STABILIZER WITH FINGER-TIGHT FITTINGS

Finger-tight Fitting Spare Parts (Order as Necessary)

Tables 2, 3, and 4 list the spare parts available for finger-tight fittings (also see Figures 2 and 3); order them as needed. The collet and ferrule are the most commonly replaced parts.

NOTE: Ferrules can be reused indefinitely as long as they function properly. Replace the ferrule only if it is damaged or fails to hold pressure.

Table 2: Finger-tight Reusable Fitting Kita

a. Use this kit when updating the column stabilizer tubing from the old-style inlet fitting to the finger-tight fitting.

Part Number Description Quantity

700003139 Finger-tight Reusable 10-32 Fitting Kit 1

Kit Components:

1/16” PEEK Ferrule (HPFT) 1

Collet (HPFT) 1

Gold Compression Screw (HPFT) 1

Collet Separator (original design, with no spiked end)

1

Table 3: Finger-tight Reusable Fitting Spare Partsa

a. Order these parts when replacing any of the existing finger-tight fitting com-ponents.

700003114 1/16” PEEK Ferrule (HPFT) 10/pk

700003115 Collet (HPFT) 2/pk

700003116 Gold Compression Screw (without holes) 2/pk

700003170 Collet and Compression Screw Multi-tool (Collet Separator)

1

205000263 Column Support Clip (Column Heater) 10/pk

205000478 Column Support Clip (Column Heater/Cooler)

10/pk

Table 4: Outlet Fitting Spare Parts

Part Number DescriptionQuantit

y

410001905 One-piece 10-32 Black Fitting (High-temper-ature Outlet Fitting)

10/pk

410001941 Flangeless Right-angle Nut Extender (Outlet Fitting Wrench)

1

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Figure 2 - Finger-tight Fittings

Tools/Materials Required• ACQUITY UPLC System Operator’s Guide (PN 71500082502)• 5/16” wrench• Collet separator (included in kit) (Figure 3)• Flangeless right-angle nut extender (outlet fitting wrench) (Figure 3)

Figure 3 - Finger-tight Fitting Tools

ProcedureThe column stabilizer tube is connected to a column or in-line filter on the column end, and to port 6 on the injector end. This procedure will outline the steps for:

• Replacing a column stabilizer tube• Installing the outlet tube• Reconnecting an old column stabilizer tube to a new column or in-line filter

Replacing the Column Stabilizer Tube

1. Open the column compartment door2. To gain access to the column heater tray:

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a. (In a column heater): Lift up the metal latch and tilt the column heater tray down-ward (Figure a).

Figure 4 - Accessing the Column Heater Tray

b. (In a column heater/cooler): Swing the column retainer rods out to the sides (Figure 5).

Figure 5 - Accessing the Column Stabilizer Tube (Column Heater/Cooler)

Removing the Old Column Stabilizer Tube (if Necessary)

1. If there is an old-style column stabilizer tube installed, remove it:a. Disconnect the outlet fitting from the column outlet.b. Disconnect the inlet fitting to the column or in-line filter.c. Disconnect the old column stabilizer from injector port 6 (Figure 6).

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Figure 6 - Disconnecting the Column Stabilizer from Port 6

Installing the New Stabilizer

1. Remove the clear laminate from the new thermal gasket adhesive back. Then, firmly apply the adhesive side of the gasket around the new stabilizer tubing block (Figure 7).

NOTE: The gasket color may vary from that shown in the image.

Figure 7 - Positioning the Thermal Gasket

2. Insert the column stabilizer tubing block into the opening on the right end of the column heater or heater/cooler tray (Figure 8).

Figure 8 - Installing the Column Stabilizer Tubing Block

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3. Remove the plastic retainer from the inlet end of the column stabilizer tube, and then connect the inlet tube to port 6 of the injector (Figure 9).

Figure 9 - Connecting the Column Stabilizer Tube

Installing the Finger-tight Fitting

1. Remove the plastic retainer from the outlet end of the column stabilizer tube; then make sure that the ferrule is about 3 mm away from the outlet tube and that the col-let is touching the back of the ferrule (Figure 10).

Figure 10 - Correct Positioning of Finger-tight Fittings

2. Push the outlet tubing into the column or in-line filter inlet until it stops.3. Holding the finger-tight reusable fitting in place, rotate the column onto the fitting

until it is snug.4. Finger-tighten the fitting until it is snug. Then add a quarter-turn.

CAUTION: TO PREVENT DAMAGE TO THE TUBING, USE ONLY YOUR FINGERS TO TIGHTEN THE FITTING.

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Installing the Outlet Fitting

1. To reconnect the outlet tubing, insert the PEEK tubing into the high-temperature out-let fitting until it stops (Figure 11).

CAUTION: TO PREVENT BANDSPREADING, ENSURE THAT THE TUBE IS FULLY BOTTOMED IN THE FITTING HOLE BEFORE TIGHTENING IT.

Figure 11 - Installing the Column

2. Holding the PEEK tubing, thread the high-temperature outlet fitting to the right until it is finger-tight.

3. Using a 5/16” wrench to hold the column steady, use the high-temperature outlet fitting wrench to give the outlet fitting a quarter turn (Figure 12)

CAUTION: TO AVOID DAMAGING THE OUTLET FITTING, DO NOT OVER-TIGHTEN IT WITH THE OUTLET FITTING WRENCH.

Figure 12 - Tightening the High-temperature Fitting

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4. Place a second high-temperature column support clip between the finger-tight reusable fitting and column, rotating the clip so that the opening faces forward (Figure 13).

Figure 13 - Installing the Second Column Support Clip

5. Dress the PEEK tubing back into the slot on the left-hand side of the column channel.6. Secure the tube:

a. (Column heater): Close the column heater tray and push down the metal latch.b. (Column heater/cooler): Swing the column retainer rod to the left to fasten it.

Reconnecting a Column Stabilizer to a New Column

NOTE: You must disassemble the finger-tight fitting before reconnecting it to a new col-umn or in-line filter.

Replacing the Column

1. Using the outlet fitting wrench, disconnect the high-temperature outlet fitting.

Figure 14 - Connecting the Column Stabilizer to a New Column

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2. Pull the column stabilizer with attached column out of the column tray.3. Grip the gold compression screw and rotate the column to detach it from the screw.

CAUTION: TO AVOID DAMAGING THE TUBING, DO NOT ROTATE THE COMPRESSION SCREW OFF THE COLUMN.

4. Replace the column (see Waters ACQUITY UPLC System Operator’s Guide).

Disassembling the Finger-tight Fitting

You must disassemble the finger-tight fitting before reconnecting it to a column.

1. Place the forked end of the collet separator over the collet on the column stabilizer tube, and gently pry the collet loose from the gold compression screw.

Figure 15 - Disassembling the Finger-tight Fitting

2. Replace the ferrule only if it is damaged or fails to hold pressure. Ferrules can be reused indefinitely as long as they function properly.

CAUTION: DO NOT REPLACE THE FERRULE WITH ANY OTHER TYPE OF FERRULE. USE ONLY THE 5/16” HPFT PEEK FERRULE (700003114).

3. Follow the steps for installing the finger-tight fitting (page 6).4. Follow the steps for installing the outlet fitting (page 7).

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Replacing the Column Stabilizer Tube with Finger …...Reconnecting a Column Stabilizer to a New Column NOTE:You must disassemble the finger-tight fitting before reconnecting it to