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Regulation of endothelial MAPK/ERK signalling and capillary morphogenesis by low-amplitude electric field
by Abdul Q. Sheikh, Toloo Taghian, Bryan Hemingway, Hongkwan Cho, Andrei B. Kogan, and Daria A. Narmoneva
InterfaceVolume 10(78):20120548
January 6, 2013
©2013 by The Royal Society
Experimental set-up for microvascular endothelial cells exposure to EF. (a) High-frequency (7.5 GHz) EF set-up: an insert with endothelial cells was placed in the small plastic dish inside the
cavity resonator to which EF was delivered from a vector network...
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
Electric field enhances angiogenic response by microvascular endothelial cells.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
Angiogenic effects of EF do not require VEGF binding to VEGFR2 receptor.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
MAPK/ERK pathway is involved in EF-mediated angiogenic response.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
High-frequency EF increases the phosphorylation of ERK, but not JNK or p38 MAP kinase in microvascular endothelial cells.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
High-frequency EF enhances MEK phosphorylation and MEK–cRaf complex formation in microvascular endothelial cells.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
Effects of PI3K and eNOS inhibition and Ca2+ chelation on EF-mediated MEK phosphorylation: (a) addition of PI3K inhibitor LY294002 did not abolish EF-induced increase in pMEK levels, when
compared with no-EF controls (n = 4, p < 0.05), suggesting that this ...
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
In vivo exposure to high-frequency EF enhances VEGF expression in diabetic wounds: (a) In vivo EF exposure set-up consisted of two antennae, which were connected to the EF source through
a flexible co-axial cable.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
(a) Angiogenic signalling pathways and (b) proposed mechanism for high-frequency EF regulation of angiogenic response.
Abdul Q. Sheikh et al. J. R. Soc. Interface 2013;10:20120548
©2013 by The Royal Society
