Recoltarea Probelor Biologice Și Patologice Amg 1

8/16/2019 Recoltarea Probelor Biologice Și Patologice Amg 1

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AMF 1- 26 MAI 2016

VIRUSOLOGIE

RECOLTAREA PROBELOR BIOLOGICE I PATOLOGICEȘ

1.Recoltarea sângelui pentru examene de laborator

Scopul:

- sta!l!"#a $!a%&ost!culu! '! (o&!to"!)a"#a "*spu&sulu! t#"ap#ut!c+

- sta!l!"#a $!a%&ost!culu! '! $!,#"#&!#"#a t!pulu! $# a(!#+

- !$#&t!,!ca"#a tulu"*"!lo" $# coa%ula"#. (o&!to"!)a"#a /#(osta)#!. a statusulu!

t"o(ol!t!c+

- #alua"#a p"#op#"ato"!#+

- !$#&t!,!ca"#a tulu"*"!lo" (#taol!c# '! #&$oc"!

P"#%*t!"#a pac!#&tulu!:- s# o,#"* pac!#&tulu! !&st"uc!u&! s!(pl#. p"#c!s#. a$aptat# &!#lulu! $# &#l#%#"#. #!t3&$

t#"(#&! (#$!cal!+

- s# #4pl!c* c#s!tat#a #4a(#&ulu!

- s# o! co&s!(*(3&tul+

- s# !&,o"(#a)* pac!#&tul cu p"!!"# la o"a"ul "#colt*"!lo" & "apo"t cu c#l#lalt# act!!t*!

$!& s#c!# 5s#"!"#a (#s#!. a$(!&!st"a"#a (#$!ca(#&t#lo". #,#ctua"#a alto" !&#st!%a!!+

- s# !&st"u!#'t# pac!#&tul cu p"!!"# la "#st"!c!!l# al!(#&ta"#. p#"!oa$a $# post 5o"#.

##&tuala

&t"#"up#"# a u&o" (#$!ca(#&t# la "#co(a&$a"#a (#$!culu! Ma7o"!tat#a t#st#lo"

"#colt#a)* & co&$!!! a)al# 5p# (3&cat#. $!(!aa $#"#(#. $up* t"#)!"# '! la (!&!(

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o"# $# la ult!(a (as* & co&$!!! $# "#paus ,!)!c '! ps!/!c+

- s# !$#&t!,!c* ##&tual# al#"%!! la susta&# $#)!&,#cta&t#. lat#4+

- s# co(at# a&4!#tat#a+

- s# o,#"* !&,o"(a!! cu p"!!"# la t!(pul c#sa" p3&* la o!"#a "#)ultat#lo"

E"o"! $# "#colta"#

- pac!#&tul &u #st# !&st"u!t co"#spu&)*to" p"!!&$ p"oc#$u"a+

- pac!#&tul &u #st# p"#%*t!t co"#ct sau &u s# "#sp#ct* "#co(a&$*"!l# p"!!&$ $!#ta.

co&su(ul

$# (#$!ca(#&t#. postu"* !&co"#ct*+- (a"a"#. #t!c/#ta"# !&co"#ct*+

- "#c!p!#&t !&a$#cat. ca&t!tat# $# p"o$us !&su,!c!#&t*+

- t"a&spo"t '! co&s#"a"# co"#spu&)*toa"#+

- #ta&'#!)a"#a "#c!p!#&t#lo". co&ta(!&a"#a p"o#lo"+

- &t3")!#"#a #4p#$!#"!! p"o$us#lo" la lao"ato". p*st"a"#a & co&$!!! co"#spu&)*toa"#+

- "#sp#cta"#a o"$!&!! $# "#colta"# 5$# u(pl#"# a ,lacoalo"+

- o(o%#&!)a"#a co"#ct* a s3&%#lu! cu a&t!coa%ula&tul9a$!t!ul $!& ,laco&+

- (#&!"#a %a"oulu! (a! (ult $# 1 (!&ut poat# (o$!,!ca o s#"!# $# "#)ultat# 5p"o$uc#

/#(oco&c#&t"a!#. (o$!,!ca"#a p"ot#!lo" plas(at!c#. act!a"#a plac/#t#lo"+

Al#%#"#a loculu!- p#&t"u puncţia capilară s# al#%# 3",ul $#%#t#lo" (#$!a& sau !la" 5$# la (3&a


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$o(!&a&t* o"! loul u"#c/!!. ,!!&$ c#sa"a p#&t"u #4a(#&ul $!"#ct 5s# $#)!&,#ct#a)a pulpa $#%#tulu!. s# !& #apa t#%u(#&tul ! s# !&$#pa"t#a)a p"!(a p!catu"a. ,!!&$ al#asaț șu"(atoa"#a ca"# s# $#pu !& ,"ot!u

- p#&t"u puncţia venoasă s# al#%# #&a $# la pl!ca cotulu! $# u&$# s# "#colt#a)a oca&t!tat# $# sa&%# c#sa"a !$#&t!,!ca"!! (!c"oo"%a&!s(#lo" $!& sa&%# 5/#(ocultu"a

2. Recoltarea exudatului faringian

Scopul:

- !)ola"#a '! !$#&t!,!ca"#a %#"(#&!lo" ca"# popul#a)* &a)o,a"!&%#l# & #$#"#a sta!l!"!!

$!a%&ost!culu! sau a st*"!! $# pu"t*to"+

- sta!l!"#a s#&s!!l!t*!! la a&t!!ot!c#+

- (o&!to"!)a"#a t"ata(#&tulu!

P"#%*t!"#a pac!#&tulu!

- s# o,#"* pac!#&tulu! !&st"uc!u&! cla"#. p"#c!s#. a$aptat# &!#lulu! $# &#l#%#"#+- & ca)ul & ca"# pac!#&tul #st# cop!l !&st"u!"#a t"#u!# ,*cut* apa"!&*to"ulu!+

- s# "#co(a&$* #,#ctua"#a "#colt*"!! &a!&t#a a$(!&!st"*"!! a&t!!ot!c#lo" $#oa"#c#

(o$!,!c* "#)ultat#l#+

- & $!(!aa "#colt*"!! pac!#&tul:

- &u (*&3&c*. &u #a+

- &u ,ac# %a"%a"*. &u-'! a$(!&!st"#a)a p!c*tu"! & &as+

- &u-'! ,ac# !%!#&a o"al*+

- s# at#&!oa)* pac!#&tul c* #st# pos!!l s* a!* s#&)a!# $# o(*

E"o"! $# "#colta"#

- "#sp#cta"#a co&$!!!lo" $# as#ps!# '! a&t!s#ps!# $#t#"(!&* co&ta(!&a"#a p"o#!+- ,olos!"#a a&t!s#pt!c#lo" o"al# poat# !&$uc# !&/!!!a $#)olt*"!! %#"(#&!lo"+

- at!&%#"#a l!(!! sau u)#lo". a $!&!lo" cu ta(po&ul poat# (o$!,!ca "#)ultatul $ato"!t*

%#"(#&!lo" #4!st#&! & ac#st# )o+

- at!&%#"#a lu#t#! poat# $#cla&'a "#,l#4ul $# o(*

E,#ctua"#a "#colt*"!!

- s# #"!,!c* $ac* pac!#&tul a "#sp#ctat "#co(a&$*"!l# p"!!&$ p"oc#$u"a+

- pac!#&tul #st# "u%at s* colao"#)# '!s* "#sp#ct# &toc(a! s,atu"!l# c#lu! ca"# "#colt#a)*+

- $ac* pac!#&tul #st# u& cop!l. po)!!asa a ,! as!%u"at* $# apa"!&*to" sau alt* p#"soa&*+

- s# o"!#&t#a)* pac!#&tul cu ,aa sp"# lu(!&*. & po)!!# '#)3&$ p#&t"u a as!%u"a o (a!

u&*

!)!!l!tat# a )o!+

- s# spal* (3!&!l# cu ap* '! s*pu&+

- s# ("ac* (*&u'!l# $# u&!c* ,olos!&*+

- s# pu (asca $# p"ot#c!#+

- pac!#&tul #st# !&!tat s* $#sc/!$* la"% %u"a. s* !&sp!"# a$3&c '! s* st#a (!'cat+

- s# scoat# ta(po&ul p#&t"u "#colta"# $!& #p"u#t*+

- cu a7uto"ul spatul#! s# apas* l!(a+

- s# 't#"%# $#po)!tul ,a"!&%!a& '! a(!%$al!a&. o"!c# )o&* !&,la(at* sau ulc#"at*+

- s# "#t"a%# ta(po&ul cu at#&!# p#&t"u a &u at!&%#l!(a. $!&!! s! u)#l#+

- s# !&t"o$uc# !(#$!at ta(po&ul & #p"u#ta p"ot#ctoa"#+- s# #t!c/#t#a)* '! s# t"!(!t# la lao"ato" co(u&!c3&$ $ac* pac!#&tul a luat (#$!ca(#&t#.


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c# ,#l

Os#"a!!P#&t"u "#colta"#a secreţiei nazale s# ,olos#'t# u& ta(po& st#"!l ,l#4!!l ca"# s# !&t"o$uc# &&a"!&* '! s# 't#"% p#"#!! lat#"al!

3. Recoltarea sputei prin expectoraţie

Scop:- !$#&t!,!ca"#a %#"(#&!lo" "#spo&sa!l! $# !&,#c!! "#sp!"ato"!! '! t#sta"#a s#&s!!l!t*!!

ac#sto"a ,a* $# a&t!!ot!c#+- $!a%&ost!ca"#a tu#"culo)#!+

- !$#&t!,!ca"#a c#lul#lo" (o$!,!cat# $# p"oc#s# !&,la(ato"!!

P"#%*t!"#a pac!#&tulu!:- s# #4pl!c* pac!#&tulu! !(po"ta&a #,#ctu*"!! $#t#"(!&*"!! '! a "#sp#ct*"!! !&st"uc!u&!lo"+

- s# !&,o"(#a)* c* p"oa s# "#colt#a)* $!(!aa. $#oa"#c# s# at#&!oa)* pac!#&tul s*&u (*&3&c#. s* &u ,u(#)#. s* &u-'! a$(!&!st"#)# p!c*tu"! & &as. s* &u s# sp#l# p# $!&!.s* &u-'! cl*t#asc* %u"a cu solu!! $#)!&,#cta&t# 5ap* $# %u"* &a!&t# $# "#colta"#+

- s# !&,o"(#a)* pac!#&tul c* $ac* a ,! o!# s# a ap#la la (#to$# ca"# s* st!(ul#)##4p#cto"a!a '! s# o! colao"a"#a+

- t#stul s# #,#ctu#a)* &a!&t#a a$(!&!st"*"!! $# a&t!!ot!c#

E,#ctua"#a "#colt*"!!:

- s# #"!,!c* $ac* pac!#&tul a "#sp#ctat "#co(a&$*"!l#+

- pac!#&tul #st# "u%at s*-'! cl*t#asc* %u"a cu ap* sau solu!# sal!&* p#&t"u a &u co&ta(!&a

sputa cu act#"!! $!& ca!tat#a ucal*+

- pac!#&tul a'#)at p# scau& sau & o"tostat!s( #st# "u%at s* "#sp!"# a$3&c $# 2- o"! '! apo!s* tu'#asc* put#"&!c+

- sputa #l!(!&at* 5#c/!al#&tul c#l pu!& al u! l!&%u"!# s# col#ct#a)* $!"#ct &

"#c!p!#&tul st#"!l 5&u s# a "#colta sal!a. c! s#c"# !a #l!(!&ata !& u"(a #,o"tulu! $# tus#+ț

- ,olos!&$ (*&u'! $# p"ot#c!# s# &c/!$# "#c!p!#&tul. s# 't#"%# #4t#"!o"ul cu u& '#"##l '!

s# !&t"o$uc# &t"-o pu&%* $# (at#"!al plast!c+

- s# #t!c/#t#a)* p"o$usul '! s# t"!(!t# la lao"ato"+

- $up* "#colta"# s# o,#"* pac!#&tulu! ap* s*-'! cl*t#asc* %u"a

E"o"! $# "#colta"#:

- sputa #st# a(#st#cat* cu sal!* sau "#stu"! al!(#&ta"# c3&$ pac!#&tul &u "#sp#ct*

"#co(a&$*"!l#.- sputa #st# a(#st#cat* cu o(a $#cla&'at* & t!(pul #p!so$ulu! $# tus#.- p"o$usul a ,ost sup"a!&,#ctat & t!(pul (a"*"!!

4. Recoltarea probelor de urină pentru examene de laborator

Scopul:

- #alua"#a ala&#! /!$"o#l#ct"ol!t!c# '! ac!$oa)!c#+

- !$#&t!,!ca"#a !&,#c!#! $# t"act u"!&a"+

- (o&!to"!)a"#a $!#"s#lo" t#"ap!!

Mo$al!t*! $# "#colta"#:

- p"#l#a"#a $!& u"!&* $# $!(!a* p"!& #(!s!a spo&ta&* $!& (!7locul 7#tulu! 5u"!&a $#$!(!a* #st# (a! co&c#&t"at* '! a"# u& p/ (a! sc*)ut+


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- so&$a7 u"!&a" st#"!l c3&$ pac!#&tul &u poat# u"!&a+

- p"!& pu&c!a sup"apu!a&* 5#,#ctuat* $# c*t"# (#$!c+

- o (ost"* $!& u"!&a #l!(!&at* '! col#ctat* & 2; $# o"# p#&t"u $o)a"#a susta&#lo" a c*"o"

#4c"#!# a"!a)* $#-a lu&%ul )!l#!

T!pu"! $# #4a(#:

- #4a(#& su(a" $# u"!&*+- #4a(# !oc/!(!c# 5Ca.


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- s# a't#apt* 1?-20 (!&ut#+

- s# $#)!&,#ct#a)* (3!&!l#+

- s# $#)!&,#ct#a)* so&$a la locul $# p"#l#a"#. ,olos!&$ alcool >0@ sau u& alt

$#)!&,#cta&t a$aptat+

- s# &#ap* so&$a cu u& ac /!po$#"(!c '! s# asp!"* ca&t!tat#a c#sa"* cu o s#"!&%* $# 20

(ld) Recoltarea urinei pentru urocultură

P"oa s# "#colt#a)* $!& u"!&a $# $!(!a* sau la c#l pu!& o"# $# la (!c!ua a&t#"!oa"*

Volu(ul p"o#! t"#u!# sa ,!# ap"o4!(at! $# 10 (l

Observaţii!!!

I& t!(pul "#colt*"!! capacul u"oculto"ulu! s# (#&t!

st#"!l

5. Recoltarea materiilor fecale pentru examenele de laborator

Scopul:

- $#p!sta"#a p#"soalo" pu"t*toa"# $# %#"(#&! pato%#&!+

- $!a%&ost!ca"#a ol!lo" !&,#c!oas# '! %"ast"o!&t#st!&al#+

- $!a%&ost!ca"#a pa"a)!to)#lo" !&t#st!&al#+

- #alua"#a #,!cac!t*t!! t"ata(#&tulu!

Mo$al!t*! $# "#colta"#:

- p"#l#a"#a $!& scau&ul #(!s spo&ta&+

T!pu"! $# #4a(#:

- #4a(#& cop"opa"a)!tolo%!c+

- #4a(#& act#"!olo%!c 5cop"ocultu"*+

P"#%*t!"#a pac!#&tulu!:

- s# a&u&* pac!#&tul s# #4pl!c* scopul '! (o$ul $# $#"ula"# a p"oc#$u"!!+

- p#&t"u cop!l s# !&,o"(#a)* ,a(!l!a+

- s# ap"#c!a)* capac!tat#a pac!#&tulu! $# a "#al!)a s!&%u" "#colta"#a+

- $ac* pac!#&tul #st# capa!l s* "#colt#)# co"#ct ! s# #4pl!c* ,oa"t# cla" #tap#l# p"oc#$u"!!+

- s# at#&!oa)* pac!#&tul s* &u a(#st#st# scau&ul cu u"!&a+

- s# !&,o"(#a)* pac!#&tul & l#%*tu"* cu "#%!(ul al!(#&ta". $u"ata ac#stu!a $ac* #st#

c#sa" postul. $ac* t"#u!# sa !a pu"%at!+

- s# !&st"u!#'t# pac!#&tul s*-'! ,ac* toal#ta p#"!a&al*

E"o"! $# "#colta"#:- pac!#&tul &u #st# !&st"u!t co"#spu&)*to"+

- pac!#&tul &u "#sp#ct* "#co(a&$*"!l# p"!!&$ $!#ta. co&su(ul $# (#$!ca(#&t#+

- ,olos!"#a "#c!p!#&t#lo" !&a$#cat#. ca&t!tat# !&su,!c!#&t* "#coltat*. "#colta"# !&co"#ct*+- co&s#"a"#a co"#spu&)*toa"#. t"!(!t#"#a & t!(p ut!l la lao"ato"+- #t!c/#ta"# !&co"#ct*

P"#l#a"#a p"o#lo":

a) Recoltarea materiilor fecale pentru examen coproparazitologic

R#colta"#a s# ,ac# $!& (at#"!!l# ,#cal# #(!s# spo&ta& ,*"* ut!l!)a"# $# pu"%at!# ul#!oas# sau

la4at!# p# a)a $# c*"u 5s# "#colt#a)a la !&$!!)!! susp!c!o&a ! $# !&,#c !! cu Asca"!s.ț țO4!u"us. Ta#&!a. #tc S# #t!c/#t#a)* "#c!p!#&tul cu &u(#l# pac!#&tulu! $ata. o"a "#colt*"!!.


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#4a(#&ul c#"ut+ s# t"!(!t# la lao"ato" & (a4!( ; o"# $# la "#colta"#+ $aca &u #st# pos!!l s# past"#a)* la ; %"a$# C#ls!us '! s# t"!(!t# la lao"ato" & (a4!( 2;o"#

b) Recoltarea materiilor fecale pentru coprocultură (examen bacteriologic)I&#st!%a!a u"(*"#'t# !)ola"#a '! !$#&t!,!ca"#a a%#&!lo" #t!olo%!c! (ot! p#&t"u ca"#

p"#l#a"#a t"#u!# ,*cut* c3t (a! ap"oap# $# $#utul ol!! '! &a!&t#a !&st!tu!"!! o"!c*"u!t"ata(#&t a&t!(!c"o!a&P"#l#a"#a s# ,ac# $!& scau&ul #(!s spo&ta& sau $!"#ct $!& "#ct 5s# "#co(a&$* & !&,#c!! la

pu"t*to"!! $# s/!%#lla sau sall(ola

6. Recoltarea secreţiei uretrale la bărbat

Scop:

- $!a%&ost!ca"#a ol!lo" cu t"a&s(!t#"# s#4ual*+

- t#sta"#a s#&s!!l!t*!! %#"(#&!lo" la t"ata(#&tul cu a&t!!ot!c#

P"#%*t!"#a pac!#&tulu!:- s# !&,o"(#a)* pac!#&tul cu p"!!"# la c#s!tat#a #,#ctu*"!! #4a(#&ulu! '! (o$ul $#

$#"ula"#+

- s# a&u&* pac!#&tul ca a ,! p*st"at* co&,!$#&!al!tat#a+

- s# as!%u"* !&t!(!tat#a

7. Recoltarea secreţiilor purulente din leziuni

Scop:

- !$#&t!,!ca"#a %#"(#&!lo" "#spo&sa!l! $# !&,#cta"#a pl*%!!+

- sta!l!"#a s#&s!!l!t*!! %#"(#&!lo" ,a* $# a&t!!ot!c#

P"#%*t!"#a pac!#&tulu!:

- s# a&u&* pac!#&tul. s# #4pl!c* c#s!tat#a #4a(#&ulu!+

- s# !&,o"(#a)* pac!#&tul c* #st# pos!!l ca p"oc#$u"a s* ,!# $u"#"oas*+

- s# a'#a)* pac!#&tul &t"-o po)!!# co(o$* & ,u&c!# $# local!)a"#a l#)!u&!!+

- s# as!%u"* !&t!(!tat#a pac!#&tulu!

8. Recoltarea secreţiilor cervico-vaginale pentru examene de laborator

a) Recoltarea pentru examenul bacteriologic şi parazitologic al secretiilor

vaginale

Scop:

#p!sta"#a $# a%#&! pato%#&! ca"# p"o$uc !&,#c!! uloa%!&al#: T"!c/o(o&as a%!&al!s.

Ca&$!$a al!ca&s. C/la(!$!a. a#(op/!lus.#tc

P"#%*t!"#a pac!#&t#!:

- &u s# ,ac !"!%a!! a%!&al# cu c#l put!& 2;/ &a!&t#a p"oc#$u"!!+

- &u s# ,olos#sc oul# sau c"#(# a%!&al# cu c#l pu!& 2 )!l# &a!&t#+

- &u s# a$(!&!st"#a)* t"ata(#&t# a&t!!&,#c!oas# cu c#l pu!& 8 )!l# &a!&t#a p"#l#*"!!+

- &u s# ,ac# p"#l#a"# $ac* #4!st* s3&%#"a"# a%!&al*

b) Recoltarea secreţiilor vaginale pentru examenul citovaginal hormonal Scop: p#"(!t# #alua"#a ,u&c!#! oa"!# p# a)a (o$!,!ca"!lo" /!stolo%!c# '! c!tolo%!c#

su,#"!t# $# #p!t#l!ul a%!&al su !&,lu#&a /o"(o&!lo" oa"!#&!


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I&$!ca!!:A(#&o"## patolo%!c*. tulu"*"! al# c!clulu! (#&st"ual. st#"!l!tat#. !&,#"t!l!tat#. $!s,u&c!!#&$oc"! cu "#p#"cu"s!u&! asup"a act!!t*!! oa"!# 5/!po,!)a. t!"o!$a. sup"a"#&al*. a,#c!u&!tu(o"al# #&!% sau (al!% (a(a"#. co&t"olul #,!cac!t*!! t"ata(#&tulu! /o"(o&al

Contraindicaţii: I&,#c!! acut# '! suacut# %#&!tal# . !&,la(at!! c#"!coa%!&al#. ca&c#"ul%#&!tal

P"#%*t!"#a pac!#&t#!:

- s# #!t* co&tact#l# s#4ual# '! sp*l*tu"!l# a%!&al# cu ? )!l# &a!&t# $# &c#p#"#a p"#l#a"!lo" '! p# pa"cu"sul ac#sto"a+

- #st# a#"t!)at* asup"a p"o%"a(ulu! $# "#colta"#:- la ,#(#!l# cu c!clul oa"!a& scu"t 521-2; )!l# p"#l#*"!l# &c#p & )!ua a >-a la

!&t#"al $# 2 )!l#+

- la ,#(#!l# cu c!clul $# 28-2 )!l# s# "#colt#a)* &c#p3&$ cu )!l#l# 8-10 la !&t#"al$# 2 )!l#+

- &u s# "#colt#a)* & ca) $# s3&%#"a"# a%!&al*

c) Recoltarea secreţiilor cervicovaginale pentru examenul citotumoral BabeşapanicolauEst# u& t#st co(pl#4 ut!l!)at p#&t"u $#p!sta"#a l#)!u&!lo" ca&c#"oas# '! a ca&c#"#lo" !&c!p!#&t# c#"!cal# la ,#(#!l# as!(pto(at!c#

I&$!ca!!:

- o $at* p# a& la ,#(#!l# & a"st* $# p#st# 18 a&! ca"# au act!!tat# s#4ual*+

- la ,#(#!l# ca"# &u p"#)!&t* l#)!u&! c#"!coa%!&al# !)!!l# p"#cu( '! la c#l# cu l#)!u&!

ca"# #oc* u& p"oc#s !&,la(ato" sau $!st"o,!c la #4a(#&ul cl!&!c sau colposcop!c

P"#%*t!"#a pac!#&t#!:

- #st# a#"t!)at* sa &u #,#ctu#)# &!c! o (a"* a%!&al* cu >2o"# &a!&t#a #4a(#&ulu!

5toal#ta a%!a&al*. apl!ca!! $# oul#. c"#(# a%!&al#+

- s# #!t* "apo"tu"!l# s#4ual# cu 2; o"# &a!&t# $# "#colta"#+

- &u s# #,#ctu#a)* (a"# ost#t"!cal# cu 2;o"# &a!&t# 5tus#u a%!&al. colposcop!#.

ta(po&a(#&t# a%!&al# #tc+

- p"#l#a"#a s# ,ac# & a,a"a (#&st"uat!#!. & a,a"a !&,#c!#! local# '! $# p"#,#"!&* &

p#"!oa$a $# (!7loc a c!clulu!

Documents

Recoltarea Probelor Biologice Și Patologice Amg 1