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Recognition of extracellular pathogens
Neutrophils Eosinophils Basophils
Mast cells
Monocytes/ Macrophages
Dendritic cells
NK cells
CD8+ Tc lymphocytes CD4+ Th lymphocytes
B lymphocytes
Myeloid
Lymphoid
PMN/ Granulocytes
Mononuclear cells
Innate
Adaptive
PRR
PAMP
eg. macrophages
Production of acute phase proteins by the liver in response to interleukin-6 (IL-6)
Liver
IL-6
C-reactive protein
Mannan- binding lectin
Other acute phase proteins, eg: SAP SP-A SP-B
Fibrinogen
Examples of Pattern Recognition Receptors (PRRs) expressed by macrophages
LPS receptor Mannose receptor
Glucan receptor
Scavenger receptor
Toll-like receptors (TLRs)
TLR6
TLR2
TLR2 TLR1 TLR4
TLR5
TLR3
TLR7
TLR8
TLR9
Peptidoglycans (Gram +) Lipoproteins Lipopolysaccharides GPI (T. cruzi) Zymosan (Yeast)
Lipopolysaccharides (Gram -) Lipoteichoic acids (Gram +) RS virus F protein
Flagellin
dsRNA
ssRNA
G-rich oligonucs
Unmeth CpG DNA
CD14
Plasma membrane
Endosome
Antigen receptor
Antigen
eg. B lymphocytes
Principles of vaccination illustrated by immunization with tetanus toxoid
Antibody response to tetanus toxin
Time
Immunization with tetanus
toxoid
Infection with tetanus
bacterium
Memory lymphocytes
present
Naïve lymphocytes
present
Antigen combining site
Classes/isotypes: IgM; IgG (1, 2, 3, 4); IgA (1, 2); IgE; (IgD) Heavy chains: µ, γ, α, ε, δ Light chains: κ, λ
Fab
Fc
Heavy chain Light chain
VH
Cµ1
Cµ2
Cµ1
Cµ2
Cµ3 Cµ3
Cµ4 Cµ4
Cε1 Cε1
Cε2 Cε2
Cε3 Cε3
Cε4 Cε4
Class
Switch IgM IgE
Antibody isotypes: distribution
IgM (pentamers) blood
IgG (monomers) blood, tissues, placental transfer
IgA blood/tissues (monomers) mucosal secretions and milk (dimers)
IgE (monomers) tissues (bound to mast cells)