Reasearch Assisstant or Biologist I or Biologist II or Senior Bi

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    EILEEN A. LARKIN

    800 Ivy Way Apt 2A (215) 206-2013

    Frederick, MD 21702 [email protected]

    PROFILE

    * Strong critical thinker and problem solver with ability to adaptto new tasks or environments quickly

    * Manage multiple projects and tasks with self-direction, excellenttime management and attention to detail

    * Work well in a team environment and independently* Excellent written and verbal communication skills, experience with

    computer and office application software

    EDUCATION

    M.S., Biomedical Science (Candidate), Hood College, Frederick, MD,Anticipated September, 2010

    B.S., Biotechnology, Shippensburg University, Shippensburg, PA, 2006

    * SciTech Scholarship (PHEAA Nets) Recipient, 2003-2006: Awarded to

    science and technology students for maintaining a 3.0 GPA andcompleting an internship* Dean's List, 2002-2005

    RELEVANT WORK EXPERIENCE

    Graduate Fellow/ Lab Technician, Integrated Toxinology Department,U.S. Army Medical Research Institute of Infectious Diseases(USAMRIID), Fort Detrick, Frederick, MD, 2006-present

    Characterization of monoclonal antibodies as potential diagnostic andtherapeutic agents against bacterial superantigens.

    * Tissue culture: maintenance of cell lines, aseptic technique, cellculture assays including T cell proliferation, InhibitionConcentration 50 percent (IC[1][Author ID1: at Tue Aug 11 14:26:002009 ][50][2][Author ID1: at Tue Aug 11 14:26:00 2009]s)[3][Author ID1: at Tue Aug 11 14:26:00 2009 ], therapeuticassays

    * Small animal testing: (mice) care, handling, injections (IP, IM,SC), euthanasia, transport, sample collection (blood, saliva,nasal, organs)

    * Tissue processing: Ficoll separation of blood culture, cellseparation from other tissue samples

    * Protein i[4][Author ID1: at Tue Aug 11 14:27:00 2009 ]nteractiontechniques: ELISA, Gel e[5][Author ID1: at Tue Aug 11 14:27:00

    2009 ]lectrophoresis and staining, Western Blot, ProteinMicr[6][Author ID1: at Tue Aug 11 14:27:00 2009 ]oarray, SurfacePlasmon Resonance

    * Bacterial culture: Plate Streaking, Colony picking/counting,Transfection, Transformation, DNA/RNA/protein harvest

    * Flow Cytometry* Radiation techniques: 3H thymidine incorporation into cells* Fast protein liquid chromatography (FPLC): Size exclusion

    chromatography* Instrumentation: GE Healthcare BIACORE 3000 and FLEXChip surface

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    plasmon resonance instruments, FACS Calibur, Array Jet and BioRadBioOdyssey Calligrapher microarray spotters, GenePix microarrayimagers, BioRad VersaDoc Imaging System, Perkin Elmer FiltermateHarvester (cell harvest), TopCount NXT (radiation counter), PerkinElmer Victor 3V (fluorescence scanner), Nexcelon BiosciencesCellometer Auto T4 (Cell counter), ELx405 Select 96 well platewasher, AKTA FPLC system

    * Software: Microsoft Office Suite, Adobe Suite, PyMOL,BIAEvaluation, Unicorn (FPLC analysis), AIRS ordering system.

    ADDITIONAL EXPERIENCE

    Counselor, GEAR UP Forensics Academy, Shippensburg University,Shippensburg, PA, June 2006

    * Taught middle school aged students about forensic science and

    biology, explained concepts, instructed a fingerprinting class andDNA isolation from cheek cells experiment

    Intern, Cumberland County Justice Department Crime Lab, Carlisle, PA,February 2006-April 2006

    * Tested evidence for presence of illegal narcotics, entered

    evidence into the computer system

    PUBLICATIONS

    Immunotherapeutcis Against Staphylococcal Toxic Shock Targeting T-cellReceptor Interactions with Exterotoxin B.

    Larkin, E.A., Stiles, B.G., Ulrich, R.G., Submitted to J. Biol. Chem.(2010)

    Larkin, E.A., Krakauer, T., Ulrich, R.G., Stiles, B.G. 2009.Staphylococcal and Streptococcal Superantigens: Basic Biology ofConserved Protein Toxins. TOTJ. 3(81): 1-81.

    Larkin, E.A., Carman, R.J., Krakauer, T., Stiles, B.G. 2009.Staphylococcus aureus: The Toxic Presence of a PathogenExtraordinaire. Curr. Med. Chem. 16: 4003-4019.

    PRESENTATIONS

    Human Monoclonal Antibodies against Staphylococcal Enterotoxin B:Potential Therapeutics?

    Seminar. Novel Therapeutics for Biothreat Agents and EmergingPathogens Series. National Cancer Institute, Frederick, MD

    Human Monoclonal Antibodies Against Staphylococcal E[7][Author ID1: at

    Tue Aug 11 14:30:00 2009 ]nterotoxin B.

    Poster session. 109^th Annual American Society of MicrobiologistsGeneral Meeting. May 17-21, 2009. Philadelphia, PA

    Hooray for Arrays: Analysis of the Superantigen Induced ImmuneResponse by Cytokine Microarray.

    Poster session. NCI Frederick and Fort Detrick Spring ResearchFestival. April 29-30, 2009. Frederick, MD.

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    Science is Fun!: An Introduction to the World of Biodefense.

    Seminar. Honors Biology Classes. Lansdale Catholic High School. March23, 2009. Lansdale, PA.

    Human Monoclonal Antibodies against Staphylococcal Enterotoxin B:Potential Therapeutics?

    Poster session. NCI Frederick and Fort Detrick Spring ResearchFestival. May 14-15, 2008.

    Frederick, MD.

    Poster session. 108^th Annual American Society of MicrobiologistsGeneral Meeting. June 1-5, 2008. Boston, MA

    COMMUNITY INVOLVEMENT

    Volunteer, FIRST Robotics Competition, 2002-present

    REFERENCES

    Upon request

    References

    1. file://localhost/tmp/wv18890.html#author12. file://localhost/tmp/wv18890.html#author13. file://localhost/tmp/wv18890.html#author14. file://localhost/tmp/wv18890.html#author15. file://localhost/tmp/wv18890.html#author16. file://localhost/tmp/wv18890.html#author17. file://localhost/tmp/wv18890.html#author1