Rapid Hit Presentation For Forensics Labs June2012

IntegenX Proprietary and Confidential | <<insert date>>D006291 Rev. A

Rapid DNAA powerful vision becoming a reality

Rapid DNAA powerful vision becoming a reality

IntegenX Proprietary and Confidential | <<insert date>>D006291 Rev. A

Advanced technology serving criminal justice

3 | IntegenX Proprietary and ConfidentialD006291 Rev. A

What is the vision for Rapid DNA Analysis? What is the vision for Rapid DNA Analysis?

Fully integrated and automated

DNA Swab Processing and

Profile Generation

Locally-oriented DNA Profile Analysis andDatabase Comparison

“High speed, simplified and automated forensic DNA analysis based on advanced technologies in DNA processing, sample analysis and database management.”

Slide 3

BJ2 Suggest "DNA Profile Analysis and Database Comparison"Sometimes, non-hit information is as informative as hit information.Bruce Jamieson, 6/19/2012


US Government Rapid DNA vision statement US Government Rapid DNA vision statement

Advance the state of the art in the collection and processing of known subject DNA. This will provide the Nation’s law enforcement agencies the capability to collect and develop an arrested individual's DNA during the normal time it takes to process a subject at intake. Such processing would initiate a search of the subject’s DNA against the Combined DNA Index System (CODIS) to determine if it matches evidence from unsolved cases. Rapid DNA will assist in preventing future crimes by expeditiously linking individuals to unsolved cases.

Joint Statement - FBI/DoD/DHS rDNA Task Force Leadership


US Government Rapid DNA vision statement US Government Rapid DNA vision statement

CONCLUSIONS

Rapid DNA is going to be an inevitable part of the future of tactical law enforcement

Tools, training and support will be available to dramatically expand the scope of how DNA analysis

is used in law enforcement


Why focus on Rapid DNA? Why focus on Rapid DNA?

• Rapid DNA delivers very fast DNA sample turnaround • DNA can be used as a tool in active investigations• The use of DNA can be extended to more crimes and

more types of crimes, including burglaries• DNA is a cost-effective solution that can help police

agencies manage investigation-related operating costs• Rapid DNA offers the opportunity for a satellite

organization that increases speed, expands coverage and multiplies forces

• Automating reference samples allows laboratory examiners to focus on more challenging crime scene samples JD1

Slide 6

JD1 Suggest "Rapid DNA increases speed, expands coverage and multiplies forces allowing laboratory examiners to concentrate on difficult crime scene samples"Joseph.DiZinno, 6/19/2012


Vision – a dramatically faster way to deliver forensic DNA results


Current Practice in Forensic DNA AnalysisCurrent Practice in Forensic DNA Analysis

10-12 hours (average)8 - 9 hrs (rush)

DetectionPCR Amplification

DilutionDNA Quantification

Cell Lysis/DNA

Extraction

Qiagen EZ1 ABI 7000RT-PCR

Tecan Genesis ABI 9700 ABI 3130Multimek

ProcessingCollection Analysis Matching

days to many weeks

Lab Time

Transport Traces Profiles

Overall Process


Rapid Forensic DNA AnalysisRapid Forensic DNA Analysis

All processes fully integrated and automated

DNA profile complete in 90 minutes or less

Compact and transportable

90 minutes

DetectionPCR Amplification

DilutionDNA Quantification

Cell Lysis/DNA

Extraction

Lab Time

Analysis


Vision - DNA as an investigative tool Vision - DNA as an investigative tool

Identify or eliminate the suspect while in custody!

• Conclusive DNA profiles obtained in 90 minutes or less

• Match or eliminate suspect swab to prior crime scene samples

• Equipment can be operated in a booking station

JD2

JD3

Slide 10

JD2 again, suggest adding " or eliminate"Joseph.DiZinno, 6/19/2012

JD3 add "or eliminate"Joseph.DiZinno, 6/19/2012


Vision - Rapid DNA extended to high-volume property crimes Vision - Rapid DNA extended to high-volume property crimes

• Career burglars average 242 burglaries a year• Career burglars commit 70% of all burglaries• Burglary is a common precedent to violent crime• Property loss averages $2200 per burglary• National burglary close rates average just 13%• Convicted burglars often get 6 months or less • DNA can be recovered from many burglary sites

Burglary Facts

• DNA identifies repeat offenders conclusively

• Convicted repeat offenders get longer sentences

• Repeat offender cases have higher clearance rates

More DNA samples mean more convictions


Case Study: Denver, CO Case Study: Denver, CO

Applying DNA Analysis to Property Crime

“Aggressive use of advanced DNA forensics in investigation and prosecution resulted in a pronounced reversal in property crimes compared to similar metropolitan areas in the United States, demonstrating the effectiveness of this approach.”

- Greggory LaBerge, Denver Police Department

-15

-10

-5

0

5

10

2003/2002 2004/2003 2005/2004 2006/2005 2007/2006

Metro Cities Denver

Burglary Trends: Denver and other Metro Cities % Change


Case Study: Palm Bay, FLCase Study: Palm Bay, FL

1101

881757

664

0

200

400

600

800

1000

1200

2006 2007 2008 2009

Started Local DNA Indexing

13%

35%

0%

5%

10%

15%

20%

25%

30%

35%

40%

Palm Bay(post-LDIS)

NationalAverage

Burglaries in Palm Bay Overall Clearance Rate


In June 2008, the NIJ released the results of a five-city field study that looked at the effectiveness of performing DNA analysis on biological evidence collected from property crime scenes.

The use of DNA for property crime - ConclusionsThe use of DNA for property crime - Conclusions

Major Findings from the DNA Field Experiment

• Suspect identifications and arrests doubled -Twice as many property crime suspects were identified and arrested when DNA evidence was collected (in addition to fingerprint evidence) compared to a traditional property crime investigation.

• Cases accepted for prosecutions doubled -More than twice as many cases were accepted for prosecution when DNA evidence was processed than when it was not.

• The suspects arrested through DNA identifications were more dangerous -DNA arrestees had double the number of prior arrests and double the prior convictions as those arrested through traditional investigations.

• DNA was twice as effective in identifying suspects as fingerprints -In cases where both fingerprint and biological evidence were collected, more suspects were identified via the FBI's Combined DNA Index System (CODIS) than were identified via the FBI's Automated Fingerprint Identification (AFIS) system.


Vision – Rapid DNA delivers significant economic benefitsVision – Rapid DNA delivers significant economic benefits


The economics of property crime – the numbersThe economics of property crime – the numbers

To the community

• San Mateo County reported 3,072 burglaries in 2009• Average loss in a burglary is $2,200• Property loss to SMC citizens in 2009 was almost $6.8 million

To the police

• Average cost to investigate a single burglary event is $491• In 2009, San Mateo County burglary investigations cost the police

budget over $1.5 million• Stopping just one career burglar will save SMC police almost

$120,000 each year


The economics of property crime - ConclusionsThe economics of property crime - Conclusions

Rapid DNA approaches help expand the use of DNA analysis to high-volume property crimes.

DNA is an effective tool for identifying and prosecuting career criminals.

Removing career criminals from the street brings significant benefit to the community and helps reduce police investigation costs.


Vision - Rapid DNA enables new law enforcement practicesVision - Rapid DNA enables new law enforcement practices

Generating DNA profiles • Fully automated system• Transportable and compact• Operable by non-forensic

personnel• Minimal training required

Analyzing DNA Results • Remote results review and

certification by forensic personnel

• Profile analysis and HIT identification

• Equipment validation, calibration and QC

• Training and supervision

“Hub and Spoke” Deployment Model


A new approach to using DNA in law enforcement A new approach to using DNA in law enforcement

“Hub and Spoke” Deployment Model


FBI Databasing structured to support local law enforcementFBI Databasing structured to support local law enforcement

NDIS

LDISFt. Lauderdale

LDISTallahassee

SDISSDISSDIS

LDISTampa


Rapid DNA -A vision becoming a

reality


Throughput IssueThroughput Issue

How can DNA analysis keep up with increased demand to support high volume crimes?

• Advanced microfluidics technology• Fully automated• Simple, reliable walk-away operation


RapidHIT™ 200: Ease of UseRapidHIT™ 200: Ease of Use

Load 4 self-contained cartridgesSample & Control cartridge

Cartridge A

Cartridge B

Sample-to-answer’ in 90 minutes


Cartridge OperationCartridge Operation

MOVe lysis solution to swabs

Patent pending



Patent pending

MOVe lysate to beads



Patent pending

Capture DNA onto beads, mix, and wash twice



Patent pending

MOVe beads to cycling region



Patent pending

Fill with premix



Patent pending

Thermocycle



Patent pending

MOVe STR amplicons and dilute with size standard



Patent pending

MOVe STR samples to inject into capillary array

electrophoresis


RapidHIT 200 Pre-Production Data 02-29-2012

RapidHIT 200 Pre-Production Run

• Sample In – Profile Out Instrument Operation• User loaded consumable cartridges

• Polymer/Separations Cartridge• Reagent Cartridge• 2 Sample Cartridges preloaded with Promega PowerPlex 16 reagents

• User inserted swabs and typed sample info in software interface• User pressed RUN and walked away• 90 minutes later, run completed• Normally, an output file would be automatically generated with called profile

• For this run, IntegenX operator hand-called alleles as software algorithms are still being trained with data sets• Automatic data analysis, allele calling, and output file generation takes 3 minutes



Sample: Allelic Ladder



Sample: Positive Control


RapidHIT ™ 200 Pre-Production Unit 4 02-29-2012

Sample 15F Buccal SwabCapillaries 4 and 8


RapidHIT 200 ™ Pre-Production Unit 4 02-29-2012

GeneMarker® by SoftGenetics automates electropherogram calls

Sample

Cap4: 15FBuccal

Alleles Pk Ht (rfu) Pk Ht (rfu) PHR(%)

D3S1358 14,16 77680 72984 94.0%

THO1 6,6 46882 n/a n/a

D21S11 31,31.2 29699 28894 97.3%

D18S51 15,20 25943 22250 85.8%PentaE 15,19 7002 6606 94.3%

D5S818 12,12 135265 n/a n/a

D13S17 8,9 52920 52481 99.2%

D7S820 10,12 41732 31712 76.0%D16S539 11,14 18198 17536 96.4%

CSF1PO 11,12 20505 15069 73.5%

PentaD 9,14 16125 17517 92.1%

Amel X,X 118651 n/a n/avWA 17,17 38678 n/a n/a

D8S1179 13,14 26025 24113 92.7%

TPOX 8,11 8523 5764 67.6%FGA 21,22 6211 5460 87.9%

Sample

Cap8: 15F Buccal

Alleles Pk Ht (rfu) Pk Ht (rfu) PHR(%)

D3S1358 14,16 76144 60773 79.8%

THO1 6,6 40027 n/a n/a

D21S11 31,31.2 22448 21036 93.7%

D18S51 15,20 20681 19256 93.1%PentaE 15,19 4098 6728 60.9%

D5S818 12,12 99511 n/a n/a

D13S17 8,9 42389 36890 87.0%

D7S820 10,12 24316 17502 72.0%D16S539 11,14 9098 8702 95.6%

CSF1PO 11,12 13061 11593 88.8%

PentaD 9,14 11747 7964 67.8%

Amel X,X 93733 n/a n/avWA 17,17 29215 n/a n/a

D8S1179 13,14 12402 9789 78.9%

TPOX 8,11 5439 7024 77.4%FGA 21,22 4639 3380 72.9%

Sample 15F Buccal Swab - Capillaries 4 and 8c


Rapid DNA – a vision becoming a realityRapid DNA – a vision becoming a reality

Fully integrated and automated

DNA Swab Processing and

Profile Generation

Locally-oriented DNA Profile Analysis and

Hit Identification

“High speed, simplified and automated forensic DNA analysis based on advanced technologies in DNA processing, sample analysis and database management.”


Rapid DNA – a vision becoming a realityRapid DNA – a vision becoming a reality

RapidHITTM 200The first fully integrated Rapid DNA analysis system

More DNA. Fewer Crimes.

How will you deploy this exciting new technology?

Documents

Rapid Hit Presentation For Forensics Labs June2012