RainbowElectrophoresis

TECHNIQUE USED TO IDENTIFY INDIVIDUALS BY THEIR DNA PROFILES

DNA Fingerprinting

The Importance of DNA Fingerprinting

PaternityTests

MedicalDiagnosis

Crime SceneInvestigation& Forensics

ForensicAnthropology

Learning Objectives

At the end of this lesson, you will be able to…

(1)Come up with ideas of how DNA fingerprinting can be applied in different areas

(2)Perform a gel electrophoresis experiment

(3)Define PCR and DNA gel electrophoresis

(4)Predict the results of the gel electrophoresis experiment according to different hypotheses

(5)Determine the answer to a case study problem by analyzing gel electrophoresis results

What are…DNA

Genes

Sequence

Polymorphic DNA Regions

Some regions of the human chromosome are polymorphic or dimorphic

e.g. Dimorphic TPA (Tissue Plasminogen Activator) gene: Some TPA alleles possess Alu elements.

Polymerase Chain Reaction

PCR (Polymerase Chain Reaction): an enzymatic amplification process

Videohttp://www.sumanasinc.com/webcontent/

animations/content/pcr.html

Gel Electrophoresis

Gel Electrophoresis:process in which molecules (e.g. DNA) are separated according to size and electrical charge by applying an electric current to the gel matrix

Videohttp://www.sumanasinc.com/webcontent/

animations/content/gelelectrophoresis.html

RainbowElectrophoresis

Questions?

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