Upload
jaeden-tippet
View
223
Download
0
Tags:
Embed Size (px)
Citation preview
Races of Races of P. graminisP. graminis f. sp. f. sp. triticitritici with virulence to with virulence to Sr13Sr13 and and Sr9eSr9e
Olivera PD, Yahyaoui A, Badebo A, Rattu A, Rouse M, Ward R, Olivera PD, Yahyaoui A, Badebo A, Rattu A, Rouse M, Ward R, Singh R, Fetch T, and Jin YSingh R, Fetch T, and Jin Y
North American Rust WorkshopNorth American Rust WorkshopSt. Paul - June 12, 2011St. Paul - June 12, 2011
Stem Rust Resistance in Durum Stem Rust Resistance in Durum
●● Durum wheat of North America has a higher frequency of resistance Durum wheat of North America has a higher frequency of resistance
to TTKSK than common wheatto TTKSK than common wheat
●● We postulated that resistance to TTKSK in durum is mostly due to We postulated that resistance to TTKSK in durum is mostly due to Sr13Sr13
●● When durum resistant selections When durum resistant selections
were evaluated in Debre Zeit, many were evaluated in Debre Zeit, many
became susceptible to stem rustbecame susceptible to stem rust
● ● Hypothesis: stem rust races in Debre Zeit Hypothesis: stem rust races in Debre Zeit
may possess a virulence combinationmay possess a virulence combination
that overcome the TTKSK resistance that overcome the TTKSK resistance
Debre ZeitDebre Zeit
Race IdentificationRace Identification
● ● SamplesSamples
-- 41 samples of infected stems collected at Debre 41 samples of infected stems collected at Debre
Zeit stem rust screening nursery in 2009Zeit stem rust screening nursery in 2009
● ● 38 s38 single pustule isolatesingle pustule isolates
-- race-typed based on the NA differential set race-typed based on the NA differential set
-- characterized on a set of 24 additional characterized on a set of 24 additional
resistant linesresistant lines
● ● Races:Races:
-- 18 Isolates 18 Isolates TTKSKTTKSK
-- 12 Isolates 12 Isolates JRCQCJRCQC
-- 4 Isolates 4 Isolates TRTTFTRTTF
Infection Types on North American Differential Infection Types on North American Differential Set of Races JRCQC, TRTTF, and TTKSK Set of Races JRCQC, TRTTF, and TTKSK
GeneGene JRCQCJRCQC TRTTFTRTTF TTKSKTTKSK
Sr5Sr5 ;1;1 44 44
Sr21Sr21 44 44 3+3+
Sr9eSr9e 44 3+3+ 44
Sr7bSr7b 22 3+3+ 44
Sr11Sr11 44 44 44
Sr6Sr6 3+3+ 3+3+ 3+3+
Sr8aSr8a 22+22+ 22 44
Sr9gSr9g 44 44 44
Sr36Sr36 00 3+3+ 00
Sr9bSr9b 2+2+ 44 44
GeneGene JRCQCJRCQC TRTTFTRTTF TTKSKTTKSK
Sr30Sr30 22-22- 44 3+3+
Sr17+13Sr17+13 33 3+3+ 22+22+
Sr9aSr9a 3+3+ 44 44
Sr9dSr9d 44 44 44
Sr10Sr10 1+3;1+3; 44 44
SrTmpSrTmp 2-2- 44 2+2+
Sr24Sr24 2-2- 22 2-2-
Sr31Sr31 2-2- 2-2- 44
Sr38Sr38 ;1;1 44 44
McNMcN 44 44 44
Infection Types Produced by Races JRCQC, Infection Types Produced by Races JRCQC, TRTTF, and TTKSK on TRTTF, and TTKSK on Sr13Sr13 and and Sr9eSr9e
JRCQC TRTTF TTKSK JRCQC TRTTF TTKSK
Sr13 Sr9e
Reaction of Race JRCQC in Durum Cultivars Reaction of Race JRCQC in Durum Cultivars
8155-B2
8155-B2
8155-B1
8155-B1
Iumillo-1
Iumillo-1
Mindum
Mindum
Leeds
Leeds
Sceptre
Sceptre
Medora
Medora
Rusty
Rusty
Khapli
Khapli
Vernal
Vernal
Kubanka
Kubanka
47-147-1
Spelmar
Spelmar
Mindum
Mindum
St464
St464
Acme
Acme
Arnaukta
Arnaukta
Infection types on Infection types on 1AL.1RS1AL.1RS of races JRCQC, of races JRCQC, TRTTF, and TTKSK TRTTF, and TTKSK
GeneGene JRCQCJRCQC TRTTFTRTTF TTKSKTTKSK
1AL.1RS1AL.1RS 2-2- 33 22+22+
JRCQC TRTTF
Race TRTTF Race TRTTF
●● Race TRTTF was first found in a collection from Yemen in 2006Race TRTTF was first found in a collection from Yemen in 2006
●● TRTTF is similar to race RRTTF that was found from stem rust TRTTF is similar to race RRTTF that was found from stem rust
collections from Pakistancollections from Pakistan
●● Race RRTTF is avirulent to Race RRTTF is avirulent to Sr9eSr9e and and 1AL.1RS1AL.1RS translocation translocation