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Quantification of Oil Present Quantification of Oil Present in Corn Masa By-product in Corn Masa By-product
StreamStream
Amy Dee SchlechteAmy Dee Schlechte
Maitri ThakurMaitri Thakur
AgendaAgenda
Introduction to Corn MasaIntroduction to Corn Masa Project ObjectivesProject Objectives ProcedureProcedure Results and DiscussionsResults and Discussions ConclusionConclusion Future RecommendationsFuture Recommendations Questions Questions
Introduction to Corn MasaIntroduction to Corn Masa
Corn is a Valuable CommodityCorn is a Valuable Commodity Starch, Cereal, Tortillas, etc….Starch, Cereal, Tortillas, etc….
Corn Masa Processing (Nixtamalization)Corn Masa Processing (Nixtamalization) Grain Cooked with HGrain Cooked with H220 & Lime0 & Lime SteepedSteeped SeparatedSeparated
Cooked Grain Cooked Grain Steep LiquorSteep Liquor
IntroductionIntroduction
Nutrient Loss in Steeping LiquidNutrient Loss in Steeping Liquid Fiber, Ash, Minerals, Proteins, OilFiber, Ash, Minerals, Proteins, Oil
Oil Extraction MethodsOil Extraction Methods Solvent Extraction using HexaneSolvent Extraction using Hexane Supercritical Fluid Extraction (SFE) using Supercritical Fluid Extraction (SFE) using
COCO22
ObjectivesObjectives
Find a Suitable Method for Oil ExtractionFind a Suitable Method for Oil Extraction
Compare Solvent Extraction & Super Compare Solvent Extraction & Super Critical Fluid Extraction MethodsCritical Fluid Extraction Methods
Determine if Oil is Concentrated in High Determine if Oil is Concentrated in High or Low Density Fraction of By-productor Low Density Fraction of By-product
ProcedureProcedure
Obtained 2 Samples - Minsa Obtained 2 Samples - Minsa Red Oak, Iowa Red Oak, Iowa
Dried Samples @ 50Dried Samples @ 50oo C for 7 Days C for 7 Days
Performed Solvent Extraction & SFE Performed Solvent Extraction & SFE
Solvent Extraction Solvent Extraction ProcedureProcedure
Combined 25g Dried Masa w/ Combined 25g Dried Masa w/ 75 ml Hexane75 ml Hexane
Filtered & Replaced Solvent 3 Filtered & Replaced Solvent 3 Times/24 HrsTimes/24 Hrs
Accumulated 1200ml of Accumulated 1200ml of Solvent Solvent
Used Rotary Evaporator to Used Rotary Evaporator to Reclaim Oil from SolventReclaim Oil from Solvent
SFE ProcedureSFE Procedure
Sample 2 Centrifuged @ 4400 RPM Sample 2 Centrifuged @ 4400 RPM for 20 Minutesfor 20 Minutes High Density FractionHigh Density Fraction Low Density FractionLow Density Fraction
SFE Performed using COSFE Performed using CO22 5 Samples (2-3g each)5 Samples (2-3g each) 2 Reps @ 42 min/cycle2 Reps @ 42 min/cycle
SFE ProcessSFE Process
Supercritical conditions 8500 psi and Supercritical conditions 8500 psi and 12012000 C C
Results & DiscussionResults & DiscussionHexane Extraction of Oil
0.05%
0.07%
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0.08
1 2
Sample
Perc
en
tag
e o
f O
il (
%)
Total oilextracted (%)
Results & DiscussionResults & Discussion
Hexane ExtractionHexane Extraction Low Percentage Oil Collected Low Percentage Oil Collected Consistent ResultsConsistent Results
Test ImplicationsTest Implications Oil Still ContainedOil Still Contained Low Oil ContentLow Oil Content
Results & DiscussionResults & DiscussionSupercritical Extraction of Oil
0.33%
0.41%0.45%
0.28%
0.72%
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
0.80P
erc
enta
ge o
f O
il (%
)
Masa 1 High Density Low Density Masa 2 Hex Ext.
Results & DiscussionResults & Discussion
SFESFE Consistent Results From Duplicate Tests Consistent Results From Duplicate Tests Greater Yield than Solvent ExtractionGreater Yield than Solvent Extraction
Test ImplicationsTest Implications Solvent Extraction + SFE = Higher Extractable Solvent Extraction + SFE = Higher Extractable
OilOil Consistent Results Consistent Results
High & Low Density Samples High & Low Density Samples Masa 1 & Masa 2Masa 1 & Masa 2
ConclusionsConclusions
Low Overall Oil Content Low Overall Oil Content
SFE vs. Solvent ExtractionSFE vs. Solvent Extraction
Synergistic EffectsSynergistic Effects
Equal Distribution of OilEqual Distribution of Oil
Future RecommendationsFuture Recommendations
Use a Different Solvent (Methanol & Use a Different Solvent (Methanol & Chloroform)Chloroform)
Perform Solvent Extraction with Perform Solvent Extraction with different ratios of Sample to Solventdifferent ratios of Sample to Solvent
Perform Additional Tests With High Perform Additional Tests With High and Low Density Fractionsand Low Density Fractions
Questions??Questions??