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Quantification and Spatial Relationship
Karsten Rodenacker,
NeuherbergMartina Hausner, München
Anna A. Gorbushina, Oldenburg
Forschungszentrumg
Content Introduction
from perception to image analysis Measurement
objects, groups of objectsinter- and intra-relationships
Examples Conclusion
Introduction Perception – recognition –
differentiation – description Quantification Relation of qualitative and
quantitative terms
Introduction The difficulty NOT
to see something
Introduction The difficulty
to see anything
Introduction The ease to see the impossible
Introduction Quantitative terms
Introduction How to relate
qualitative and quantitative terms?
Introduction Digitisation Segmentation
Introduction Sub sectioning and change of scale
Introduction
Extension Size Shape Structure
Measurement (of one object)
Introduction
Arrangement Relation Neighbourhoo
d
Measurement (of several objects)
Examples of measurements,objects and groups of objects
Spatial relationships
Measurement
Measurement Area Perimeter Extension
Measurement Shape Growth shape Density,
intensity
Measurement Extensions
Length (skeleton)=1621 pxmean thickness=2.27 px
Measurement Neighbourhood
closing onfilaments
Measurement Spatial
RelationshipDelaunay triangulationnearest neighbours
minimum spanning treeconvex hull
Skeleton neighbourhood
Measurement Spatial
RelationshipExample frompathology
Measurement Spatial relationship (objects of different
type)
Distances to the red phaseDouble marked sludge flocks
Measurement Measurement
continuum Measurement
hierarchy
pixel contentlocation properties
object
object
object
object
objectcontent
locationexternal properties
Example Bacterial growth in flow chambers
Differentiation of wild and mutant bacteria pseudomonas aeruginosa by CLSM imaging
Example Wild (PA) and
mutant (MW) bacteria
Growth overtime (slice # = depth)
Example Substrate coverage (closing)
Wild type bacteria Mutant bacteria
Example Bacterial growth in flow chambers
Conjugativegenetic transfer in bacterialbiofilm
Example Quantification of colonies of micro
colonial fungi from sub aerial biofilms coniosporium sp. and sarcinomyces sp. under soil (b), sand (s) coverage and in air (l)
Example Colonies of micro colonial fungi
Example Colonies of micro colonial fungi
Conclusion Perception, description
and measurement of objects and object groups in images
Exclusions (e.g. texture, filtering, fractals, etc.)
Faith and (apparent) truth