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6FraunhoFer eMB
INDUSTRIAL CELL TECHNOLOGY
SympoSium10-11/09/2015
6TH ANNUAL MEETING
PROGRAM• LüBeck (D)
QUALITY CONTROL
LIVE CELL IMAGING
BIOPROCESSING TECHNIQUES
Dear Ladys and Gentlemen,Dear Colleagues and Friends,
This year, the 6th annual meeting for industrial cell technology is hosted at the new
Fraunhofer building in Luebeck. Its focus is on key strategies, which help to bring
cell and tissue based technologies to industrial scales. The field is rapidly evolving.
Advances have been made in new sensors, in ever improving consumables and
single-use devices, in software for cell-logistics and documentation, in a plethora of
defined media and 3D-matrices, to name but a few.
This year, the focus will be on three main topics: Bioprocessing Techniques, Live Cell Imaging and Quality Con-
trol. The field of Bioprocessing Techniques pertains to new developments for the standardized and controlled
mid- to large-scale production of cells and micro tissues in bioreactors. This encompasses not only the biore-
actors themselves but also new media, new matrices, carriers or fibers and sterilization techniques.
Live Cell Imaging has established itself as a powerful and highly flexible technique for the in situ monitoring
of cell cultures. Considerable progress has been made in recent years, both in hard- and software. For instance,
lenseless devices and the ever falling prices for data storage permit the inexpensive automated recording of
thousands of cell images. New algorithms turn those images into numbers for the detailed analysis of cell
cultures.
Quality Control pertains mainly to the wide field of sensor applications in cell culture and other cell based
technologies. New multi-mode sensors have been invented in the last years. These permit the simultaneous
measurement of many different parameters directly in the culture medium or within the cells. At the same time,
the cost for sensors has dropped significantly, making it possible to control and monitor cells in a broad range
of applications. The symposium aims to identify new platform technologies for the industrial cell technology to
make new contacts and to foster cooperation.
We welcome you to our symposium.
Prof. Dr. rer. nat. habil. Charli KruseDirector of the Fraunhofer Research Institution for
Marine Biotechnology EMB
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Organizer and SPOnSOrS
ORGANIZER
SPONSORS
LIFESCIENCE
NORD
01 Fraunhofer EMB, Lübeck
02 Life Science Nord Management GmbH, Hamburg
03 Cenibra GmbH Life Science Solutions, Bramsche
04 Eppendorf AG, Hamburg
05 Carl Zeiss Microscopy GmbH, Jena
06 Essen BioScience, Mülheim a. d. Ruhr
07 microdrop Technologies GmbH, Norderstedt
Exhibitors
LIFESCIENCE
NORD
Posterausstellung
Counter
6th Annual Meeting I Industrial Cell Technology I Symposium
10-11 September 2015
68 PAX
01 = Fraunhofer EMB02 = LSN03 = Cenibra04 = Eppendorf05 = Carl Zeiss06 = Essen Bio07 = microdrop08 = tba 09 = Meintrup DWS10 = Sarstedt11 = Fraunhofer Mevis12 = Nikon13 = Biozym14 = ibs | tecnomara15 = Binder
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03
04
05
06
09
10
11
12
13
14
15
07
02
08
Stage 4 m x 3 m
Entrance
Entrance
Lounge
09 Meintrup DWS Laborgeräte GmbH, Lähden
10 Sarstedt AG & Co., Nümbrecht
11 Fraunhofer MEVIS, Lübeck
12 Nikon GmbH - Microscope Solutions, Düsseldorf
13 ibs | tecnomara GmbH, Fernwald
14 Biozym Scientific GmbH, Hess. Oldendorf
15 Binder GmbH, Tuttlingen
INDEX
Organizer and Sponsor
Exhibitors
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PROGRAm | THuRSDAyPROGRAm | FRIDAy
9
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SESSION l BIOPROCESSING TECHNIQuESDr. Elmar Schmälzlin
Dr. Marek Höhse
Dr. Elena Meurer
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14
16
SESSION ll LIVE CELL IMAGINGDr. Frank Fischer
Dr. Derek Trezise
Dr. Stephan Schmidt
Dr. Tim Becker
Dr. Nico Scherf
Dr. Joël Mailliet
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SESSION lll QuALITy CONTROLDr. Karin Schütze
Dr. Eric Nebling
Dr. yousef Nazirizadeh
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Contacts
Information
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SCIENTIfIC MANAGEMENTFraunhofer Research Institution for Marine Biotechnology EMB
CONfERENCE VENUEFraunhofer Research Institution for Marine Biotechnology EMB Mönkhofer Weg 239aD-23562 Lübeck
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THuRSDAy 10, 2015
Setup of
exhibition booths
14:00 - 17:00
GET-TOGEThER
with live music
17:00
Welcome
Cabaret (german)
17:15
19:00
Prof. Dr. Charli Kruse
Director of the Fraunhofer EMB (D)
Congress President
Bernd Saxe
Mayor of the Hanseatic City of Lübeck (D)
PROGRAM
SESSION lll | QUALITY CONTROL
SESSION ll | LIVE CELL ImAGING
SESSION l | BIOPROCESSING TEChNIQUES
1110
FRIDAy 11, 2015
Registration 08:00
WELCOmE 08:45 moderation: Dr. Daniel Rapoport
Fraunhofer Research Institution for Marine Biotechnology EMB
Wolfgang-Dieter Glanz
Ministry of Economic Affairs, Employment, Transport and
Technology
SESSION l
Bioprocessing Techniques
09:00-
09:20
09:20-
09:40
09:40-
10:00
Dr. Elmar Schmälzlin
Colibri Photonics GmbH
„Non- invasive optical oxygen measurements
within cell tissue using phosphorescent microspheres“
Dr. marek höhse
Sartorius Stedim Biotech GmbH
„Spectroscopic online monitoring and control of
cell cultivation processes“
Dr. Elena meurer
apceth GmbH & Co KG
„Translation of cell-based gene therapy into
clinical application“
Exhibition + Coffee Break 10:00-
11:00
Atrium
SESSION ll
Live Cell Imaging
11:00-
11:20
11:20-
11-40
11:40-
12-00
Dr. Frank Fischer
Beiersdorf AG
„Mitochondrial dynamics in living keratinocytes“
Dr. Derek Trezise
Essen BioScience Ltd.
„Evolved quantitative methods for cell surveillance and
monitoring using non-invasive kinetic live cell imaging“
Dr. Stephan Schmidt
Institute of Organic and Macromolecular Chemistry
Heinrich-Heine-university
„A universal affinity assay based on the
adhesion of soft microparticles“
PROGRAM
OPEN QUERY 12:00 The next big thing in Cell Technology
Lunch Break + Exhibition 12:30-
13:30
Atrium
SESSION lll
Quality Control
13:30-
13:50
13:50-
14:10
14:10-
14:30
Dr. Tim Becker
Fraunhofer Research Institution for Marine Biotechnology EMB
„Image based cytometry for adherent cell cultures“
Dr. Nico Scherf
Max Planck Institute of Molecular Cell Biology and Genetics
„Towards systematic, real-time developmental biology“
Dr. Joël mailliet
BioTek Instruments GmbH
„Tumour Invasion Assays using 3D Spheroids and
Invasion Matrix“
Exhibition + Coffee Break 14:30-
15:00
Atrium
15:00-
15:20
15:20-
15:40
15:40-
16:00
Dr. Karin Schütze
CellTool GmbH
„Label-free and non-invasive quality control of
cell based therapeutics“
Dr. Eric Nebling
Fraunhofer Institute for Silicon Technology ISIT
„Diagnostics and quality control with
silicon based chip technology“
Dr. Yousef Nazirizadeh
Byosens GmbH
„Label-free detection for cellular assays“
End of Symposium 16:00
Bioprocessing Techniques
SESSION I
13
Dr. Elmar SchmälzlinCEOColibri Photonics GmbHPotsdam-Golm, Germany
Non- invasive optical oxygen measurements within cell tissue using phosphorescent microbeads
Molecular oxygen plays a central role in the metabolism. The control of oxygen is important for cell breeding. Changes can be related to metabolic responses, e.g. for toxicity tests. Since cells consume oxygen, the concentration within tissue is lower than in the supernatant culture medium.
To obtain meaningful values, it is crucial to measure within the tissue.However, previous techniques do not allow non-destructive measurements inside cell clusters. Needle-type sensors injure the tissue. The result is interfered by stress, diffusion through the puncture and needles cannot be inserted into closed channels. Sensor spots are indeed suitable for closed systems, but only indicate the oxygen content on the border of a cell layer.
An optical oxygen measurement system called OPAL fills this gap. It consists of phosphorescent spherical microprobes and a hardware unit, which is intended to be linked to a microscope. The biocompatible sensor beads are overgrown by the cells. Ambient oxygen quenches the sensor signal, i.e.intensity and decay time decrease. To determine the oxygen-dependent decay time, phase modulation can be applied. The excitation light is sinusoidally intensity modulated and the phase shift of the sensor signal is measured.
However, in the case of faint microsensors this method is substantially disturbed by superposing background fluorescence, which unavoidably arises from biological samples. To fade out fluorescence a special two-frequency phase modulation technique was applied. This method allows in-situ oxygen measurements in biological environments using extraordinary small probes.
1514
Dr. marek höhseScientist Sartorius Stedim Biotech GmbHGoettingen, Germany
Sensors, Data Evaluation and Automation for Comprehensive Bioprocess Control
The QbD/PAT approach shows great benefit in classical pharmaceutical industry. With regard to upstream bioprocesses as cell cultivation and microorganism fermentation as well as downstream processes, reliable online analytic and a lack of automation still hamper the successful implementation of the QbD/PAT concept. Monitoring the critical process parameters during biotechnological cell cultivations is of high importance for maintaining a high efficiency and quality of a bioprocess. For some parameters, such as pH-value or the concentration of dissolved oxygen, commercial sensor systems for real time inline monitoring are available. For others like glucose concentration, cell count or viability a robust online prediction is in many applications not yet state of the art. New sensor developments and concepts for bioprocess applications are presented. This includes e.g. analytical systems for online glucose, lactate and viable biomass. Furthermore the capabilities of multi-parameter techniques as Near Infrared Spectroscopy are demonstrated, going far beyond quantitative analysis of single parameters. In combination with powerful chemometric technologies, these spectroscopic analyzers allow for multivariate statistical process control and thus event based sampling.Furthermore Multivariate Data Analysis provides an overview of the complete bioprocess in total. The combined evaluation of fused sensor-, process- and quality control data reveals parameter interactions and gathers comprehensive process understanding. The results are displayed in a few easy to comprehend graphs and allow for the online detection of process deviations. In combination with innovative automation concepts direct bioprocess control is achieved, thus mitigating process risks.
Co-Authors: José Alves-Rausch, Christian Grimm
PERSONAL NOTES
1716
Dr. Elena meurerHead of Quality Controlapceth GmbH & Co. KGOttobrunn, Germany
Translation of cell gene based therapy into clinical application
apceth is a pioneering biopharmaceutical company in the field of cell and gene therapy. apceth has been developing next generation (genetically engineered) cell products aimed for the treatment of solid cancers and non-malignant diseases for which no satisfactory treatment options are available today. To this end apceth has created a modular platform that combines MSCs with different therapeutics genes. The therapeutic genes are chosen in such a way to target the specific cellular mechanisms of a chosen disease.
The first-in-class, first-in-men clinical study with genetically modified autologous MSC-therapeutic Agenmestencel-T for cancer treatment has recently progressed from a phase I (safety) to a phase II (efficacy). It is the worldwide first clinical application of genetically modified MSCs and the first report that MSCs have been used in oncology. Also, a recent switch from the autologous platform, based on patient-derived MSCs, to the allogeneic platform, based on MSCs derived from healthy volunteers, will enable start of the clinical trial phase I/II with apceth‘s second cancer therapeutic Agenmestencel-L at the end of 2015. Agenmestencel-L is based on genetically modified MSCs obtained from heathy donors (allogeneic MSCs) and will be therefore available as an off-the-shelf product. For development of genetically modified MSC, apceth combines scientific know-how with state-of-the-art technologies and highest GMP standards and provides the in-house expertise for all stations of pharmaceutical path, from early preclinical stages, over GMP manufacturing to their clinical validation.
PERSONAL NOTES
19
Dr. Frank FischerHead of Microscopy Lab Beiersdorf AGHamburg, Germany
Mitochondrial Dynamics in Living Keratinocytes
As a microscopy lab in an industrial setting we are forced to provide a whole bunch of microscopic methods for the applications needed by our R&D department. In terms of live cell imaging in very high resolution we cannot use electron micros-copic methods. But, we can move superresolution light microscopic technologies now available. For example, using a ground state depletion (GSD) microscope combined with total internal reflection fluorescence microscopy (TIRF) and wide field fluorescence microscopy we were able to visualize and quantify mitochondrial network dynamics in living keratinocytes. We quantitatively analyzed area and structure of the mitochondrial network in fixed and living keratinocytes. We were able to show significant effects of substances known to influence mitochondrial network dynamics or mitochondrial membrane potential.
Co-Authors: Robin Sieg, meike halm, Sonja Pagel-Wolff, martin Sattler
PERSONAL NOTES
LIVE CELL IMAGING
SESSION II
20
Dr. Derek TreziseDirector & European Site HeadEssen BioScience, Ltd.Welwyn Garden City, united Kingdom
Evolved, quantitative methods for cell surveillance and monitoring using non-invasive kinetic live-cell imaging
Cell biologists and biotechnologists are increasingly working with more advanced and precious cell systems. These include stem-cell derived differentiated human cells, engineered patient-specific cells for adoptive immunotherapy, recombinant cell lines for therapeutic protein production and co- and multi-culture models to reconstitute complex biology. Developing, optimising and maintaining robust and reproducible cell handling and differentiation protocols is now an obligate science. This presentation will cover the application of long term, non-invasive and quantitative live-cell imaging methods to track and optimise cell protocols over time. Examples of how this approach increases efficiency in reprogramming and differentiating stem-cells and can accelerate cell-based assay development and workflow will be provided.
PERSONAL NOTES
Dr. Stephan SchmidtInstitute of Organic and Macromolecular Chemistry Heinrich-Heine-universityDüsseldorf, Germany
A universal affinity assay based on the adhesion of soft micro particles
We establish a new sensing principle that uses ligand / receptor functionalized hydrogel particles as sensors. The principle is based on the idea that hydrogel microparticles form well defined contact areas upon adhesion on a chip surface modified with a corresponding binding partner. When adding an analyte the contact area decreases depending on analyte concentration and affinity, see figure. So far we have mainly focused on analyzing weak carbohydrate interactions using optical microscopy as a detection technique.[1] In addition we used this method to establish several assays covering a broad range of analyte classes, including: antigens and antibodies, cell adhesion proteins [2] and metal ions [3]. Overall we can state three main characteristics of the method:• Highly sensitive screening method with ELISA-like workflow but with greatly reduced incubation times.• Suited for highly parallel formats (well plates) and automated high-throughput screening.• Flexible: Can be easily adapted to construct various assays for different applications and analytes.
In addition, the hydrogel particle assay mimics certain aspects of cellular adhesion. Therefore we currently use this method to shed light on cell adhesion phenomena and recognition processes at soft, multivalent biointerfaces.
[1] Pussak, D. et al. Angewandte Chemie International Edition (2013), 52, (23), 6084 [2] Martin, S. PCCP (2015), 17, (5), 3014[3] Schmidt, S. et al. Biomacromolecules (2014), 15, (5), 1644.
Patent filed: PCT / EP 2014 / 050163
mapping: Schematic of the adhesion measurement and analyte screening via hydrogel particles.
22
Dr. Tim BeckerProject ManagerFraunhofer Research Institution for Marine Biotechnology EMBLübeck, Germany
Image based cytometry for adherent cell cultures
Timelapse microscopy is the preferred and only method to observe an adherently growing cell population in an non-invasive, i.e. label-free manner. This technique allows for the observation and quantification of different processes such as directed cell migration, mitosis distribution and cell differentiation. The acquired image sequences store all information that are needed to create a spatio-temporal characterization of the recorded cell families. But while the image recording can easily be performed using an automated microscope, the required image processing and data analysis still remains challenging: a completely automated image-based cytometry is aggravated by the low-contrast of microscopical image data, by the variability of used techniques such as phase contrast, darkfield or holographic microscopy.
To overcome this issue, we implemented an automated data validation algorithm for cell tracking data. We combined the validated data with an tracking independent mitosis detection to unravel the knots of the cell trajectories caused by the non-linear behaviour, i.e. the cell division.
using these algorithms, a were able to extract a wide range of different cellular parameters such as growth curves, complete genealogical trees and cell cycle times with a very high precision. Altogether, our validation algorithms allow for an automated cell tracking and the extraction of biologically meaningful parameters from in vitro cell cultures.
PERSONAL NOTES - OPEN QUERY
2524
Dr. Nico ScherfMax Planck Institute of Molecular Cell Biology and GeneticsDresden, Germany
Towards a systematic, real-time, developmental biology
Over the centuries, microscopy has always been a crucial technique in our quest to learn about the inner workings of biological systems. Many of our insights today rely on the direct observation of processes in the chosen model systems. But the architecture of multicellular life is complex: it is formed by an orchestrated interplay of cells in a hierarchical process across different scales in space and time. ultimately, this complexity is beyond what we can visually comprehend by simply looking through a microscope. A thorough understanding of life requires more than traditional, visual inspection; it requires quantitative measurements and statistics on a larger ensemble to judge the variability and significance of the findings. This poses a number of challenges on how to acquire, handle, and analyze the relevant information. To reveal the invisible, we need more than just good optics.
As a first step into this direction, I present our image-based analysis of endoderm formation in zebrafish. In our selective plane illumination microscope (SPIM) setup we recorded the development of multiple embryos in parallel to obtain a map of all endodermal cells for each embryo. By exploiting the spherical geometry of an early zebrafish embryo, we computed cartographic projections of the entire endodermal layer and visualized the respective cellular distributions and flows. The integration of these data from different samples then revealed stereotypic patterns of endodermal tissue formation. We think that following the route presented in this work will get us closer to our goal: systematic, quantitative imaging of complex, living specimen.
Co-Authors: Gopi Shah, Benjamin Schmid, Jan huisken
Dr. Joël maillietProduct Specialist BioTek Instruments GmbHBad Friedrichshall, Germany
Tumour Invasion Assays using 3D Spheroids and Invasion Matrix
Metastasis is the main cause of death in cancer patients and one of the most complex biological processes in human diseases. Target-based approaches that center on cancer gene mutations are fraught with difficulties as the development of cancer involves a series of mutations, some of which drive the disease, while others are just passengers. Phenotypic approaches that involve 3D cell culture methods that model tumour invasion may provide a more successful approach to developing new therapies. Here we demonstrate a method for the generation of 3D spheroidal tumour structures using co-cultures of cells, creation of a suitable invasion matrix, and image-based monitoring and analysis of tumour invasion.
PERSONAL NOTES
QUALITY CONTROL
SESSION III
27
Dr. Karin SchützeCEO, Scientific DirectorCellTool GmbHBernried, Germany
Label free and non-invasive cell and tissue examination
IntroductionRaman spectroscopy (RS) is a highly sensitive analytical method for marker-free and non-invasive identification and characterization of cells. Here, we present RS as a new tool for gentle yet precise cell analysis in three independent experiments, focusing on cell differentiation and identification. We could provide evidence that RS is a suitable tool to follow up differentiation of human stem cells and is able to successfully distinguish between skin-graft cultured fibroblasts, melanocytes and keratinocytes in 2D and 3D cell systems.
material & methodsTo analyze stem cell differentiation, in a first experiment adipose derived stem cells (ASCs) were purified from lipoaspirates and cultivated for 0 or 7 days in differentiation medium allowing differentiation to chondrocytes. For Raman measurement, cells were fixed with PFA and subsequently analyzed. In a second experiment, neuronal progenitor cells (NPCs) were isolated from spinal cord of rats, grown under proliferation conditions and treated with 10ng/ml TGF-β1 on day 1, 4 and 7. Afterwards, samples were fixed with 4% PFA for analysis with Raman spec-troscopy. Finally, the capability of RS to discriminate between cell types was shown using fibroblasts, melanocytes and keratinocytes, which were cultured several days under proliferation conditions in 2D cell culture or on a 3D skin graft and subsequently fixed in PFA for Raman analysis.
ResultsRS could clearly differentiate between ASCs and chondrocytes. Raman analysis furthermore revealed that 60% of the fibroblast cells were differentiated to chondrocytes, showing higher levels of collagen. using NPCs, Raman spectra clearly discriminated TGF-β1 treated neurons from vehicle-treated ones. Indeed, RS could
assign TGF-β1 induced changes in the Raman spectrum, in particular in proline and RNA amounts. RS did also provided evidence that in the primed setup some cells still have the character of non-primed cells. In the third experiment, RS could clearly differentiate between fibroblasts, melanocytes and keratinocytes in the 2D and 3D setting, providing evidence that differences between keratinocytes and melanocytes are mainly due to different amounts in phenylalanine and lipid content.
DiscussionRS is a photonic marker for gentle yet highly specific cells analysis. It allows to follow cell differentiation and is a suitable tool to discriminate between cell types. In addition, it provides information about the entire metabolome of a single cell that is as characteristic as a “fingerprint”. With this, RS can be used for quality assessment of cells without impairing cell viability.
AcknowledgementThis project has received funding from the European union’s Seventh Program for research, technological development and demonstration under grant agreement No 279288.
Co-Authors: Kremling h.1, Klein B.23, Aigner L.23, Oberbauer E.4, Wolbank S.4,
marino D.5, meyer S.5
1CellTool GmbH, Bernried, Germany2Institute of Molecular Regenerative Medicine, Paracelsus Medical university Salzburg, Austria, 3Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS),
Paracelsus Medical university Salzburg, Austria4Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Linz, Austria5Tissue Biology Research unit, Department of Surgery, university Children`s Hospital, Zürich, Switzerland
29
PERSONAL NOTES
3130
Dr. Eric NeblingHead of Department Biotechnical MicrosystemsFraunhofer Institute for Silicon Technology ISITItzehoe, Germany
Diagnostics and quality control with silicon based chip technology
The department “Biotechnical Microsystems (BTMS)” of the Fraunhofer ISIT is one of the leading groups in the field of electrical biochip technology. The electrical biochips offer intrinsic advantages because of particle tolerance and mechanical robustness by the direct transduction of biochemical reactions into electrical current. Different sensor types will be presented:
- Silicon based biosensors are used for the continuous monitoring, e.g. of metabolites as glucose or lactate. The monitoring and quantification of these substances is realized by enzymatic conversion and electrochemical detection. These sensors could be used in combination with pH-measurement and -control in bioreactors. - For a wider range of mobile analytics, ISIT develops microsystems based on a liquid chromatographic separation process. The aim of this development is an integrated microsystem for detection of contaminants and residues for a sustainable environment, food and health management.
- Micro pore chips with surrounded pH-sensitive electrodes will be used for the detection and activity testing of transmembrane proteins. These proteins will be embedded into lipid bilayer membranes which covers the micro pores.
- The use of gold electrode arrays combined with integrated reference and auxiliary electrodes along with sensitive, selective measurement techniques like “Redox-Cycling” enables powerful sensor systems for the Point-of-Care diagnostics, food- and environmental control.
Dr. Yousef NazirizadehManaging DirectorByosens GmbHKiel, Germany
Label-free detection for cellular assays
Revealing biological interactions using fluorescent dyes, radioisotopes or conjugated enzymes has a long tradition. There are, however, issues such as misfolding, reduced mobility, or steric hindrance, which can impact the bioactivity of the labeled biological system. Moreover the pricy reagents, time consumable handling and the incompatibility with living cells are burdens on the success of many projects.In comparison, label-free detection shows to be the more native, simpler and more physiological alternative. Especially in living cells assays label-free detection can deliver real-time data about the biological process, which traditionally is measured at an end-time measurement.
We present an optical method based on grating sensors that measures the refractive index. As it can be used for experiments involving a mass change on the surface of the sensor, this method is a general tool for cellular assays, such as adhesion and migration assays, proliferation and cytotoxicity assays and cell signaling and cell communication assays. Beyond these assays any other experiment involving a mass change on the sensor surface can be performed in real time. We discuss results from G protein–coupled receptor (GPCR) assays, where a dynamic mass redistribution, which is a redistribution of cellular matter, can be observed.
PERSONAL NOTES
3332
Session l
Dr. Elmar SchmälzlinCEOColibri Photonics GmbHPotsdam-Golm, [email protected]
www.colibri-photonics.com
Dr. marek höhseSartorius Stedim Biotech GmbHDep. PAT | Sensors & Chemometrics R&D Instrumentation & ControlGoettingen, Germany [email protected]
www.sartorius.de
Dr. Elena meurerHead of Quality Controlapceth GmbH & Co. KGOttobrunn, [email protected]
www.apceth.com
ContaCts
Session ll
Dr. Frank FischerHead of Microscopy Lab Beiersdorf AGHamburg, [email protected]
www.beiersdorf.com
Dr. Derek J TreziseDirector & European Site HeadEssen Bioscience, Ltd.Welwyn Garden City, united [email protected]
www.essenbioscience.com
Dr. Stephan Schmidt Institute of Organic andMacromolecular Chemistry Heinrich-Heine-universityDüsseldorf, Germany [email protected]
www.hhu.de
Dr. Tim BeckerProject ManagerFraunhofer Research Institution for Marine Biotechnology EMBLübeck, [email protected]
www.emb.fraunhofer.de
Dr. Nico ScherfMax Planck Institute of Molecular Cell Biology and GeneticsDresden, [email protected]
www.mpi-cbg.de
Dr. Joël maillietProduct Specialist BioTek Instruments GmbHBad Friedrichshall, [email protected]
www.biotek.de
Session lll
Dr. Karin Schütze CEO, Scientific DirectorCellTool GmbHBernried, [email protected]
www.celltool.de
Dr. Eric Nebling Head of DepartmentBiotechnical MicrosystemsFraunhofer Institute for Silicon Technology ISITItzehoe, [email protected]
www.isit.fraunhofer.de
Dr. Yousef NazirizadehManaging DirectorByosens GmbHKiel, [email protected]
www.byosens.com
3534
VENUE
Fraunhofer EmB, Mönkhofer Weg 239a, 23562 Lübeck1
1
INFORMATION
Fraunhofer Research Institution for marine Biotechnology EmB
Mönkhofer Weg 239a23562 Lübeck, Germany
Director Prof. Dr. rer. nat. habil. Charli Kruse
For questions concerning the congress and the program please contact:
Jessica Barnewitz, B.Sc.Fraunhofer EMBPhone +49 451 384448-31
annabell ringkewitzFraunhofer EMBPhone +49 451 384448-58
