Upload
others
View
1
Download
0
Embed Size (px)
Citation preview
QTL associated with adaptation to Mediterranean Dryland conditions in the barley cross Arta x Keel
L Sanchez Alvarez, J Rollins, M Baum, S Ceccarelli, S Grando, M von KorffMax Planck Institute for Plant Breeding Research, Köln, GermanyInternational Center for Agricultural Research in the Dry Areas, ICARDA, Syria
Introduction and ObjectivesThe study aims at identifying the genetic basis for agronomic
performance in dry Mediterranean environments. We use a QTLapproach to map different agronomic traits recorded in the field andphysiological and protein changes in the RILs grown under drought inthe greenhouse.
The different environments analyzed showed a large variation intemperature and rainfall distribution which resulted in significantgenotype by environment interactions. Developmental andmorphological traits affected grain yield differently in differentenvironments which was reflected in a number of QTL by environmentinteractions.
QTL main effects across all environments were only detected for plantheight, peduncle length, kernel weight and growth vigor. Grain yieldunder drought was mainly affected by flowering time. Epistaticinteractions between different parental alleles at Vrn-H1, Vrn-H2 and aQTL on 7HL caused late flowering and a concomitant decline in yield.
A recombinant inbred barley population derived from Arta x Keel was grown at twodifferent field locations in Syria from 2006-2008 including additional springplantings in 2007 and 2008 at both locations. A mixed model approach was used todetect QTL and marker by environment interactions. The population is currentlytested for differences in physiological traits and protein expression under controland drought conditions in the greenhouse.
Results
QTL Analysis for Agronomic Traits under Drought in RILs (Arta x Keel)
Thousand Kernel Weight
0
5
10
15
20
25
Control Drought
Tho
usa
nd
Ker
nel
Wei
ght
Arta Keel
AB
C
D
Water Use Efficiency
0
0.5
1
1.5
2
2.5
Control StressGra
in w
eigh
t (g
)/w
ater
(kL)
Arta Keel
AA
BB
1HHvM36
GBM1052
scssr10226
HvHOTR1
Bmag829Bmag813
Bmag720Bmac126
GMS03Ebmac684
Bmag140
Bmag378
Hv5s
Ebmac415
Bmag749scssr8447
HY03I05T3
S53707
GBM1007
GMS021
Bmag770
Bmag718
Bmac501Bmag105a
Bmag382
GBM1461
HvLTPPBEbmac705
Scssr10559
Bmag828
Bmac209
Scssr25691
HVM60
GBM1501
HvM40
GBM1323
Ebmac906Bmag740
Bmac310
HY03C23T
Ebmac679Ebmac701
HvM67
Bmag419B
Scssr7106
Bmag5Bmag751
Bmac96
Bmac163
HVLEU
Bmag222
HK03No2R
Scssr9398
HvM74
HvPLASCB1
Bmag767
HvM4
Bmag11
Bmag120
GBM1464
BMS64
Ebmac603
Ebmac755
Hvm49
Bmag135
GMS1HvM6
scssr103
2H 3H
HVM62
Bmag606
MGB358
4H 5H 6H 7H
Arta increases trait valueKeel increases trait value
Heading date- main effect
Heading date – interaction effect
GBM5162
otto21
GMS149 GBM1469
GBM1047
GBM1143
Bmag306
VRNH2
GBM1176
DH
DH
DH
DH DHVRNH1
DH
DH
DH
DH
DH
HvFT3
DHAF043094A
Scssr10148
GBM1400
Bmag812
HK05l13r
GMS61
Scind60002
GMS6
GBM1021
GBM1075
GBM1399
GBM1270
GBM1215
Bmag496
Bmag419A
VRN3_FT1
GBM1482
HvMLOH1AGBM1256 DH
DH
Arta Keel
RIL Population
Genotyping
Agronomic traits
•Australian Feed Barley, drought tolerant•No vernalisation requirement
•Syrian Landrace, adaptedto to driest sites in Syria•Vernalisation requirement
x
Physiological traitsAgronomic traits
Protein and phosphoprotein changes
Field Controlled Conditions
Phenotyping
LEBANON
LATAKIA
TARTUS
Bairut
DAMASCUS
DARA'ASUWEIDA
1200
1000
1400
300
1000
200
600
500
400
400
400
500500
350
350
350300 300
300250
200
150
100
500600
800
1200
1000
1400
PALMYRA
DEIR EZZOR
HASSAKEHALEPPO
HAMA
HOMS
DAMASCUS
SUWEIDA
RAQQA
BredaTel Hadya
TerbolKfardane
IRAQ
JORDAN
TURKEY
Med
iter
rane
an S
ea
L L
211.7 170.4Annual rainfall (Sept-Mai)
0
50
100
150
200
250
300
2006 2007 2008
mm
ann
ual r
ainf
all
Tel HadyaBreda
Distribution of annual rainfall (Sept-Mai)
020406080
100
S O N D J F M Amonths
mm
rai
n 200620072008
The RIL population was trialled at Tel Hadya and Breda, Syria from 2006-2008. Theenvironments were characterised by moderate to severe drought stress. Rainfalldecreased from 2006 to 2008, in particular during the major growth months February,March.
1HHvM36
GBM1052
scssr10226
HvHOTR1
Bmag829Bmag813
Bmag720Bmac126
GMS03Ebmac684
Bmag140
Bmag378
Hv5s
Ebmac415
Bmag749scssr8447
HY03I05T3
S53707
GBM1007
GMS021
Bmag770
Bmag718
Bmac501Bmag105a
Bmag382
GBM1461
HvLTPPBEbmac705
Scssr10559
Bmag828
Bmac209
Scssr25691
HVM60
GBM1501
HvM40
GBM1323
GBM1482
Ebmac906Bmag740
Bmac310
HY03C23T
Ebmac679Ebmac701
HvMLOH1A
Bmag419A
HvM67
Bmag419B
Scssr7106
Bmag5
Bmag751
Bmac96
Bmac163
HVLEU
Bmag812
HK05l13r
GMS61AF043094A
Scssr10148
Bmag222
HK03No2R
Scssr9398
Scind60002
GMS6
HvM74
HvPLASCB1
Bmag767
HvM4
Bmag11
Bmag120
GBM1464
BMS64
Ebmac603
Ebmac755
Hvm49
Bmag135
GMS1HvM6
scssr103
2H 3H
GBM1021
GBM1075
GBM1256
HVM62
Bmag606
MGB358
4H 5H 6H 7H
Vrn-H1
Main effects across environmentsArta increases trait valueKeel increases trait value
Marker * environment interaction
DH = Heading dateGY =Grain yieldKW = Kernel weightPH = Plant height
PEDEX = Peduncle extrusionGV = Growth vigour
GBM5162
otto21
GMS149 GBM1469
GBM1047
GBM1143
Bmag306
VRNH2
GBM1176
GBM1399
GBM1270
GBM1215
Bmag496
GBM1200
VRN3_FT1
GV
GV
GV
GV
GV
KW
KW
KW
KW
KW
PEDEX
PEDEX
PEDEX
PEDEX
PEDEXPH
PH
PH
PH
PH
PH
PH
PH
PH
DH
DH
DH
DH
DH
DH
DH
DH
DH
GY
GY
GY
GY
GY
GY
GY
GY
GY
Epistatic interactions for flowering
Analysis of epistatic interactions revealed interactions between QTLs and markersnot significant in the single marker analysis. The QTL at Vrn-H2 showed the largestnumber of epistatic interactions. Interactions between different parental alleles atVrn-H1, Vrn-H2 and a QTL on 7HL explained 50% of the genetic variance, causedlate flowering and a concomitant decline in yield.
Drought reduced kernel weight in Arta and Keel, while Keel showed higher kernel weightunder control and drought conditions. Keel was characterised by a higher water useefficiency than Arta.
ab
c
d
a
b
a
b