QCVN ve VSV

7/26/2019 QCVN ve VSV

1/12

QUY CHUN K THUT QUC GIA

I VI NHIM VI SINH VT TRONG THC PHM

National technic al regulat ion

of Microbiologicalcontaminants in food

I. QUYNH CHUNG

1. Phm vi iu chnh

Quy chun ny quy nh mc gii hn v nhim vi sinh vt trong thc

phmi vi cc sn phm hoc nhm sn phm : sa v sn phm sa; trng v

sn phm trng; tht v sn phm tht; thy sn v sn phm thy sn; sn phm dinh

dngcng thc dnh cho tr t 0 n 36 thng tui; nc khong thin nhin ng chai,

nc ung ng chai v nc dng lin; kem; rau, qu vsn phm rau, qu (sau y

gi tt l thc phm) v cc yu cu qun l c lin quan .

2.i tng p dng

Quy chun ny p dng i vi:

2.1. Cc t chc, c nhn xut nhp khu, sn xut, kinh doanh cc thc phm quy

nh ti khon 1.

2 .2 . C quan qun l nh nc c lin quan.

3. Gii thch tng v k hiu vit tt

Trong Quy chun ny cc t ng v k hiu vit tt di y c hiu nhsau:

3.1. Gii hn nhim vi sinh vt trong thc phml mcgii hnti avi sinh vt

cphp c trong thc phm.

3.2. Phn loi ch tiu

Ch tiu loi A: l ch tiu bt buc phi kim nghim khi tin hnh nh gi

hp quy.

Ch tiu loi B: l ch tiu khng bt buc phi kim nghim khi tin hnhnh

gi hp quynu nh sn xut thc hin kim sot mi nguy trong qu trnh sn xut


2/12

QCVN 8-3:2012/BYT

4

(theo HACCP hoc GMP). Trong trng hp nh sn xut khng p dng kim sot

mi nguytrong qu trnh sn xut th bt buc phi kim nghimcc ch tiu ny.

3.3. K hiu vit tt

- n: s mu cn ly t l hng kim nghim.

- c: s mu ti a cho php c kt qu kim nghim nm gia m v M. Trong n

mu kim nghim c php c ti a c mu cho kt qu kim nghim nm ga m

v M.

- m: gii hn di, nu trong n mu kim nghim tt c cc kt qu khng vt

qu gi tr m l t.

- M: gii hn trn,nu trong n mu kim nghim ch 01 mu cho kt qu vtqu.gi tr M l khng t.

- TSVSVHK: Tng s vi sinh vthiu kh.

- KPH: Khng pht hin.


3/12

QCVN 8-3:2012/BYT

5

II. QUYNH KTHUT

1.Gii hn nhim vi sinh vt trong sa v sn phm sa

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp

(CFU/ml hocCFU/g)

Phn loich tiu

n c m M

1.1Cc sn phmsa dng lng

Enterobacteriaceae 5 2 < 1 5 A

L. monocytogens(1) 5 0 102 A

1.2 Cc sn phmsa dng bt

Enterobacteriaceae 5 0 101 A

Staphylococci dngtnh vicoagulase

5 2 101 102 A

Ni c t caStaphylococcus(Staphylococcal

enterotoxin)

5 0 KPH (2) B


Salmonella 5 0 KPH (2) A

1.3 Cc sn phm phomat

1.3.1 Phomat c snxut t sa tinguyn liu

Staphylococci dngtnh vi coagulase

5 2 104 105 A

Ni c t ca

Staphylococcus(Staphylococcalenterotoxin)

5 0 KPH (2) B



Ghi c h:: (1)i vi sn phm dng ngay

(2)trong 25g hoc 25ml


4/12

QCVN 8-3:2012/BYT

6

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp

(CFU/ml hocCFU/g)

Phn loich tiu

n c m M

1.3.2 Phomat c snxut t sa qua x l nhit

E. coli 5 2 102 103 A


5 2 102 103 A


enterotoxin)

5 0 KPH (2) B



1.3.3Phomat whey(sn xut t whey

qua x lnhit)

E. coli 5 2 102 103 A


5 2 102 103 A


enterotoxin)

5 0 KPH (2) B


1.3.4Phomat tic sn xut tsa hoc whey

(sa hoc whey qua x lnhit)


5 2 101 102 A

Ni c t caStaphylococcus

(Staphylococcalenterotoxin)

5 0 KPH

(2)

B


1.3.5 Cc sn phmphomat khc


Ghi c h:: (1)i vi sn phm dng ngay

(2)

trong 25g hoc 25ml


5/12

QCVN 8-3:2012/BYT

7

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp

(CFU/ml hocCFU/g)

Phn loich tiu

n c m M

1.4 Cc sn phm cht bo t sa

1.4.1 Cream v b

E. coli 5 2 101 102 A



1.4.2 Cht bo sa,du b, cht bosa tchnc, du b tch nc v chtbo t sa dngpht


1.5 Cc sn phm sa ln men

1.5.1 Cc sn phmsa ln men qua x l nhit

Enterobacteriaceae 5 2 < 1 5 A


1.5.2 Cc sn phmsa ln menkhng qua x lnhit


2.Gii hn nhim vi sinh vt trong trng v sn phm trng

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp

(CFU/ml hocCFU/g)

Phn loich tiu

n c m M

2.1 Cc sn phmtrng

Enterobacteriaceae 5 2 101 102 B


Ghi c h: : (1)i vi sn phm dng ngay

(2)trong 25g hoc 25ml


6/12

QCVN 8-3:2012/BYT

8

3.Gii hn nhim vi sinh vt trong tht v sn phm tht

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp (CFU/g) Phn loi

ch tiu

n c m M

3.1Tht v sn phmch bin t thts dng trc tipkhng cn x lnhit

TSVSVHK 5 2 5x105 5x106 B

E. coli 5 2 5x101 5x102 B


3.2Tht v sn phm

ch bin t thtphi qua x lnhit trc khi sdng

TSVSVHK 5 2 5x105 5x106 B

E. coli 5 2 5x102 5x103 B


3.3 Gelatine vcollagen


4.Gii hn nhim vi sinh vt trong thy sn v sn phm thy sn

TTSn phm

Ch tiuK hochly mu

Gii hn chophp (CFU/g)

Phn loich tiu

n c m M

4.1

(tunicates)cnsng

E. coli 1 0 230(3) 700(3) B


4.2

gia nhit

E. coli 5 2 1 101 B


5 2 102 103 B


Gh i ch:: (2)

trong 25g hoc 25ml(3)MPN/100g c tht v nidch


7/12

QCVN 8-3:2012/BYT

9

5.Gii hn nhim vi sinh vt trong sn phm dinh dng cng thc dnh cho trt 0 n 36 thng tui

TT Sn phmCh tiu K hochly mu Gii hn chophp (CFU/g) Phn loi

ch tiun c m M

5.1

Sn phm dinhdng cng thcsng btcho trn 12 thng tui


Enterobactersakazakii

30 0 KPH (4) A

Enterobacteriaceae 10 0 KPH (4) B

Bacillus cereus gi nh 5 1 5x101 5x102 B

5.2

Sn phm dinhdng cng thcvi cc mc ch yt c bit cho tr

n 12 thng tui


Enterobactersakazakii

30 0 KPH (4) A


Bacillus cereus gi nh 5 1 5x101 5x102 B

5.3

Sn phm dinhdngcng thcvi mc ch ndm cho tr t 6n 36 thng tui



5.4

Thc phm chbin t ng cccho tr t 6 n36 thng tui

Coliform 5 2 < 3 20 A


Ghi c h:: (2)trong 25g hoc 25ml(4)trong 10g hoc 10ml


8/12

QCVN 8-3:2012/BYT

10

6. Gii hn nhim vi sinh vt trong rau, qu vsn phm rau, qu

TT Sn phm Ch tiu

K hochly mu

Gii hn chophp (CFU/g) Phn loi

ch tiun c m M

6.1 Rau mm (n ngaykhng qua x lnhit)


6.2 Rau n sng

E. coli 5 2 102 103 B

Salmonella 5 0 KPH(2)

A

6.3 Qu n ngay

E. coli 5 2 102 103 B


7. Gii hn nhim vi sinh vt trong kem

TTSn phm

Ch tiu K hochly mu

Gii hn chophp (CFU/mlhoc CFU/g)

Phn loich tiu

n c m M

7.1 Kem

(i vi cc loikem c cha sa)

Enterobacteriaceae 5 2 101 102 B


Ghi c h:: (2)trong 25g hoc 25ml


9/12

QCVN 8-3:2012/BYT

11

8. Gii hn nhim vi sinh vt trong nc khong thin nhin ng chai, ncung ng chai v nc dng lin

8.1 Kim tra ln u

TT Ch tiu Lng mu (ml) Yu cuPhn loich tiu

8.1.1 E. colihoc coliformchu nhit

1x 250 KPH A

8.1.2 Coliformtng s 1x 250

Nu s vi khun (bo t)

1 v 2 th tin hnh kimtra ln th2.

Nu s vi khun (bo t)>2 th loi b

A

8.1.3 Streptococci fecal 1x 250 A

8.1.4 Pseudomonasaeruginosa

1x 250 A

8.1.5 Bo t vi khun kkh kh sulfit

1x50 A

8.2 Kim tra ln th hai

TT Ch tiu

K hoch ly muGii hn cho php

(CFU/ml)Phn loich tiu

n c m M

8.2.1 Coliformtng s 4 1 0 2 A

8.2.2 Streptococci fecal 4 1 0 2 A

8.2.3 Pseudomonasaeruginosa

4 1 0 2 A

8.2.4 Bo t vi khun kkh kh sulfit

4 1 0 2 A


10/12

QCVN 8-3:2012/BYT

12

III. PHNG PHP LY MU V PHNG PHPTH

1. Ly mu

Ly mu theo hng dn ti Thng t 16/2009/TT -BKHCN ngy 2 thng

6 nm 2009 ca B Khoa hc v Cng ngh v hng dn kim tra Nh nc

v cht lng hng ho lu thng trn th trng v cc quy nh khc ca

php lut c lin quan.

2. Phng php th

Yu cu k thut quy nh trong Quy chun ny c th theo cc

phng php di y (cc phng php ny khng bt buc p dng, cth s dng phngphp th khc tng ng) :

- TCVN 4884: 2005 (ISO 4833:2003) Vi sinh vt trong thc phm v thc n

chn nui - Phng php nh lng vi sinh vt trn a thch - K thut m khun

lc 300C.

- TCVN 4829: (ISO 6579: 2002) Vi sinh vt trong thc phm v thc n chn

nui - Phng php pht hin Salmonellatrn a thch.

- TCVN 7924-1: 2008 (ISO 16649 -1: 2001) Vi sinh vt trong thc phm v

thc n chn nui - Phng php nh lng Escherichia coli dng tnh -

glucuronidaza, Phn 1: K thut m khun lc 44oC s dng mng lc v 5-

bromo-4-clo-3-indolyl -D-glucuronid.


thc n chn nui - Phng php nh lng Escherichia coli dng tnh -

glucuronidaza, Phn 2: K thut m khun lc 44oC s dng 5-bromo-4-clo-3-

indolyl -D-glucuronid.


thc n chn nui- Phng php nh lng Escherichia coli dng tnh -

glucuronidaza, Phn 3: K thut m s c xc sut ln nht s dng 5-bromo-4-

clo-3-indolyl- -d-glucuronid.


11/12

QCVN 8-3:2012/BYT

13

- TCVN 7700-2: 2007 (ISO 11290-2:1998, With amd 1: 2004) Vi sinh vt trong

thc phm v thc n chn nui - Phng php pht hin v nh lng Listeria

monocytogenes,Phn 2: Phng php nh lng.

-TCVN 4830-1:2005 (ISO 6888-1:1999, with Amd, 1:2003) Vi sinh vt trong

thc phm v thc n chn nui - Phng php nh lng Staphylococcic phn

ng dng tnh vi coagulase (Staphylococcus aureusv cc loi khc) trn a

thch, Phn 1: K thut s dng mi trng Baird-Parker.

- TCVN 4830-2:2005 (ISO 6888-2:1999, with Amd, 1:2003) Vi sinh vt trong

thc phm v thc n chn nui - Phng php nh lng Staphylococcic phn

ng dng tnh vi coagulase (Staphylococcus aureusv cc loi khc) trn a

thch, Phn 2: K thut s dng mi trng thch fibrinogen huyt tng th.

- TCVN 4830-3:2005 (ISO 6888-3: 2003) Vi sinh vt trong thc phm v thc

n chn nui - Phng php nh lng Staphylococci c phn ng dng tnh vi

coagulase (Staphylococcus aureusv cc loi khc) trn a thch, Phn 3: Pht

hin v dng k thut m s c xc xut ln nht (MPN) m s lng nh.

- TCVN 6191-2:1996 (ISO 6461-2:1986) Cht lng nc Pht hin v

m s bo t vi khun k kh kh sulfit (Clostridia), Phn 2: Phng php mng

lc.

- TCVN 4882:2007 (ISO 4831: 2006) Vi sinh vt trong thc phm v thc n

chn nui - Phng php nh lng coliforms - K thut m s c xc sut ln

nht.

- TCVN 6848:2007 (ISO 4832: 2006) Vi sinh vt trong thc phm v thc n

chn nui - Phng php nh lng coliforms- K thut m khun lc.

- TCVN 5518-1:2007 (ISO 21528-1: 2004) Vi sinh vt trong thc phm v

thc n chn nui - Phng php pht hin v nh lng Enterobactericeae, Phn

1: pht hin v nh lng bng k thut MPN c tin tng sinh.

- TCVN 7850-2008 (ISO/TS 22964:2006) Vi sinh vt trong thc phm v thc

n chn nui - Phng php pht hin v nh lng Enterobacter sakazakii.

- ISO 16266:2006 Water quality Detection and enumeration of
http://www.iso.org/iso/iso_catalogue/catalogue_ics/catalogue_detail_ics.htm?ics1=13&ics2=60&ics3=70&csnumber=39272http://www.iso.org/iso/iso_catalogue/catalogue_ics/catalogue_detail_ics.htm?ics1=13&ics2=60&ics3=70&csnumber=39272


12/12

QCVN 8-3:2012/BYT

14

Pseudomonas aeruginosa Method by membrane filtration (Cht lng nc

Pht hin v nh lng Pseudomonas aeruginosaPhng php lc mng).

- ISO 7899-2:2000 Water quality Detection and enumeration of intestinal

enterococci, Part 2: Membrane filtration method (Cht lng nc Pht hin v

m khun lin cu khun ng rut, Phn 2: Phng php lc mng).

IV. QUY NH V QUN L

Cc thc phm quy nh ti Mc II phi c kim tra cht lng, an ton

m bo nhimvi sinh vtkhng vt qu gii hn quy nh ti Quy chun ny.

i vi cc thc phm c quy nh trong Quy nh gii hn ti a

nhim sinh hc v ha hc trong thc phm ban hnh km theo Quyt nh s46/2007/Q-BYT ngy 19/12/2007 ca B Y tnhngcha c quy nh trong

Quy chun k thut ny s tip tc p dng theo Quyt nh s 46/2007/ Q-BYT.

V. TRCH NHIM CA T CHC, C NHN

T chc, c nhn ch c php sn xut, kinh doanh v xut khu, nhp khu

cc thc phm ph hp vi gii hn nhim vi sinh vt c quy nh ti Quy

chun k thut ny.

VI. TCHC THC HIN

1.Giao Cc An ton v sinh thc phm ch tr, phi hp vi cc c quan chc

nng c lin quan hng dn trin khai v t chc vic thc hin Quy chun ny.

2.Cn c vo yu cu qun l, Cc An ton v sinh thc phm c trch nhim

kin ngh B Y t sa i, b sung Quy chun ny.

3. Trong trng hp cc tiu chun v quy nh php lut c vin dn trong

Quy chun ny c s thay i, b sung hoc c thay th th p dng theo vn

bn mi.
http://www.iso.org/iso/iso_catalogue/catalogue_ics/catalogue_detail_ics.htm?ics1=07&ics2=100&ics3=20&csnumber=14854http://www.iso.org/iso/iso_catalogue/catalogue_ics/catalogue_detail_ics.htm?ics1=07&ics2=100&ics3=20&csnumber=14854

Documents

QCVN ve VSV