PWG LaboratoriesAAALAC Accredited GLP Research Laboratories

SpecializedServices inNon-clinical Research

A Reliable Partner of Your Discovery

PWG Genetics Pte Ltd

PWG Laboratories in Singapore by PWG Genetics was established in 2005 to provide excep-

tional preclinical research services, animal model PWG SPF Micropig, NHP and conventional animal models to the growing biomedical research community.

OECD/US FDA GLP Compliant study services in the area of drug and medical device development including toxicology/pharmacology, bioanalysis and ISO10993 Bio-compatibility Testing as well as discovery, effi cacy trials are off ered with a high scientifi c quality.

AAALAC accredited Micropig/Animal research facilities are also equipped with MRI, Micro-CT/PET, C-Arm X-ray and angiography enabling state-of-the-art animal imaging required for multi-disciplinary investigations.

PWG Laboratories

Offi ce / Customer ServiceTraining Center

4F

3F

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1F

PWG GENETICS PTE LTD

Animal Care Unit 1Breeding Center

Study Area / LaboratoryAnimal Care Unit 2

Laboratory / Bioimaging CenterSpecial Experimental Unit (BSL3)

15 Tech Park Crescent, Singapore 638117

Phone: +65 6863 3042Fax: +65 6863 4834Email: info@pwgpig.comWeb: www.pwg.com.sg www.pwgmicropig.com

GLPGood Laboratory

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www.pwg.com.sg

PWG Micropig®

Micropig, as the NEXT GENERATION animal model

There are no single animal species duplicating all the characteristics of human biology and

diseases but PWG Micropig® is the next generation ani-mal model that comes close to the this unmet need in bio-medical research, enhancing translational potential of the research results into human with safety and practicality.

PWG Micropig becomes popular as the most ap-propriate animal model for biomedical re-

search as well as in teaching and training.

PWG Micropig have a slow to moderate growth of about 2 kg or less per month based on their special genetic, manage-ment and nutrition programs. The three common types of PWG Micropig® that are recognized and maintained based on their typical growth weight development;

M (Middle)-type, T (Tiny)-type and ET (Extra Tiny)-type.

Growth Curve

OriginPWG Micropig belongs to the ge-

nus Sus and species scrofa. It is a product of synthetic or composite mix-ture of various subspecies, domestic, native and miniature breeds and strains of swine that is purposely bred to be docile, easy to handle, compact in size and an excellent laboratory animal for biomedical research.

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Why PWG Micropig?

1. Scientific and Ethical Reasons

Anatomical, Physiological and Metabolic Similarities to human - Skin, Gastrointestinal Tract, Cardiovascular System, Metabolism, Urogenital System, Teratogenic Sensitivity and Juvenile Development Lower ethical concernsAccepted as non-rodent species for regulatory studies

2. High health status, Specific-Pathogen-Free (SPF) purpose-bred

Enhanced data credibilityMicrobiologically defi ned for specifi c pathogensMinimum risk of zoonosis Prevent adverse eff ects on research results caused by clinical disease and antibodies

3. Smaller size, slow growth rate and early maturity

Small size but large enough for scientifi c/biological manipulation, samplempling and investigationBirth weight ~0.5kg or lessOnset of sexual maturity reaches as early as 3 months of ageSubtypes (M, T and ET) with diff erent growth rate to choose from depending on the purpose of researchMaintain body weight and size throughout adulthood similarly like adult human

4. Advantages

Possible to use all standard routes of administration and samplingLess amount of drug material required; more suitable for drug discovery Long term studies possible without burdens in drug material cost or animal handling.

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PWG Genetics Pte Ltd

Standard Care of Micropig

Environmental Conditions

Temperature: 20-30 degrees CelsiusHumidity: 40-80%Class: 1,500-10,000GLP; 100,000 animal roomsAir changes: 10-15 per hour. no air re-circulationPressure: -3- -5 (negative) mmHgAir Velocity: 20-25cm/secNoise: Up to 60dBLight Intensity: 150-300, 12 Light:12Dark cycle

Health Monitoring Program

PWG health monitoring program is a compre-hensive animal herd screening conducted

in house regularly to maintain microbiologically defi ned, healthy micropig herd. Certain numbers of animals are randomly selected per animal room for sampling and to represent the herd considering all variation. Various samples are collected from chosen animal depending upon the pathogen being tested.

Viral Infections

Aujeszky’s Disease/Peudorabies (PRV) Classical Swine Fever/Hog Cholera (CSFV) Japanese Encephalitis (JEV)Porcine Circovirus (PCV)Porcine Parvovirus (PPV) Porcine Reproductive & Respiratory Syndrome (PRRSV)

Bacterial Infections

Actinobacillosis Actinobacillus pleuropneumoniae (A2, A5) Actinobacillus suis Streptococcus suis Atropic Rhinitis (AR) Bordetella bronchiseptica Pasteurella multocida (Type A, D) Haemophilius, Glasser’s disease Haemophilus parasuisErysipelas (SE), Diamond-skin disease Erysipelothrix rhusiopathiaeLeptospirosis Leptospira sppMycoplasma, Swine enzootic pneumonia (SEP) Mycoplasma hyopneumoniaeSalmonellosis Salmonella typhimurium Salmonella choleraesuis

Parasistic Infections

Ectoparasites/Arthropods (Lice, mites, ticks)Endoparasites/Helminths (Worms, fl ukes)Toxoplasmosis (Toxoplasma gondii)

www.pwg.com.sg

Our Services & Scientific Expertise

GLP General ToxicologyClinical Pathology and

Analytical Study Bioresrouces Supply

Juvile ToxicologyEfficacy Studies

in Disease ModelsSurgery & Bioimaging

* Single and Repeated Dose* Acute, Subchronic & Chronic* Oral, Dermal* Parenteral Routes - bolus IV, continous infusion, SC, IM* Standard Measurement* On-site Quality Assurance

* Hematology (ADVIA2120, Precil)* Serum Chemistry (Dimension Xpand Plus)* Urinalysis (Clintek Advantus)* Histopathology (H&E, Special Staining IHC)* Formulation Analysis (LCMSMS, HPLC)

* Micropigs and NHP* Research Animals* Blood* Serum* Plasma* Skin* Organ & Tissues

* Diabetes* Parkinson’s Disease* Atherosclerosis* Osteoporosis* Tumorigenicity Studies

* Catheterization* Medical Device Implantation* Post-Op Care* MRI* Micro-CT/PET* C-Arm X ray

Pharmaco/ToxicokineticsSpecialty Research

(BSL3)

* For Pediatric Indications* All routes and measurement parameters* Enhanced Enrichment and Handling

GLPGood Laboratory

Practice

PWG Genetics Pte Ltd

ISO10993-5 CytotoxicityMEM Elution Agar DiffusionMTT EvaluationColony Formation

Medical Device Biocompatibility Testing

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Biocompatibility Testing Services of Medical Devices

ISO10993-3 GenotoxicityAmes Test (Bacterial Mutagenicity) Mose Lymphoma AssayMouse Micronucleus TestChromosome Aberration Test

ISO10993-4 HemocompatibilityHemolysisProthrombin Time/Partial Thromboplastin TimeComplement ActivationPlatelet Aggregation, Count, Activation

in vivo Thrombogenicity

ISO10993-10 Sensitization & Irritation

Guinea Pig Maximization TestRepeated Patch TestPrimary Skin Irritation Murine Lymphnode Assay

ISO10993-9 Implantation Acute / Sub-Acute (Sub-Chronic) / Chronic

ISO10993-11 Systemic ToxicityAcute / Sub-Acute (Sub-Chronic) / Chronic

Biocompatibility testing is necesssary to evalutate the medical devices that are typi-

cally subjected to determine the suitability of a device for human use and measure

potentially harmful physiological eff ects. PWG Laboratories, now provide the excep-

tional biocompatibility testing services, in various aspects listed above.

www.pwg.com.sg

PWG Genetics Pte Ltd15 Tech Park Crescent, Singapore 638117

Phone +65 6863 3042Fax +65 6863 4834

Email info@pwgpig.comWeb www.pwg.com.sg

www.pwgmicropig.com