© 2 0 1 2 C o r n i n g I n c o r p o r a t e d P r i n t e d i n U . S . A . 3 / 1 2 P O D C L S C G T S 3 4 5 Puromycin Dihydrochloride Puromycin dihydrochlori de is isolated from Streptomyces alboniger as a broad-spectrum aminonucleoside antibiotic. Puromycin dihydrochlori de is active against gram-positive bacteria and weakly active against gram-negative bacteria. The mechanism of action invol ves premature release of nascent polypeptide chains by the addition of Puromycin dihydrochloride t o the growing peptide chain. Protein synthesis is inhibited in both prokaryotic and eukaryotic cells that do not express the puromycin-N-acety l-transferase (pac) gene encoding resistance. Puromycin d ihydrochloride i s as effective as Hygromycin B and G418, and selection can be achieved in 2-3 days. Stock solutions may be prepared by dissolving the antibiotic in deionized water . When filter-sterilized (0.22 micron filter), stock solution may be stored at 4°C for up to 1 year. The stock solution may also be divided into aliquots and s tored frozen for greater stability . The effective killing concentrati on of the antibiotic wil l vary as to cell type, media, growth conditions, density, and the cell’ s metabolic rate and position in the cell cycle. When using puromycin dihydrochloride in a new cell system, a full dose curve is suggested, and with each new lot of puromcin dihydrochloride, several points on that curve should be retested. Molecular Structure Product Specificati ons Molecular Weight 544.4 Formula C 22 H 29 N 7 O 5 * 2HCl Mode of Ac tion Aminoacyl tRNA mimic, cau sing premature polypeptide chain termination. Conferred Resistance Aminoglycosid e-modifying enzyme s, a change in cell permeability, or a change in ribosomal structure. Spectrum Gram (+) Gram (-) bacilli aerobes and facultative anaerobes, only. Purity ≤ 98% Effective Concentration 0.5 to 10 µg/mL Appearance White to off-white powder Storage Frozen (-15 to -25°C), protected from light Cat. No. Description Size Qty/Pk 61-385-RA Puromycin Dihydrochloride, powder 100 mg 1 Mediatech, Inc. A Corning Subsidiary 9345 Discovery Blvd. Manassas, VA 20109 t 800.235.5476 f 703.471.0363 www.cellgro.com For a complete listing of trademarks, visit us at www.cell gro.com/about-us/tr ademarks.html orning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001 ® cellgro ® The Corning Family of Brands ® ® Corning cellgro products are manufactured by Mediatech, Inc., a Corning Subsidiary

Puromycin Dihydrochloride

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Puromycin DihydrochloridePuromycin dihydrochloride is isolated from Streptomyces alboniger as a broad-spectrum aminonucleoside antibiotic. Puromycin dihydrochloride is active against gram-positive bacteria and weakly active against gram-negative bacteria.

The mechanism of action involves premature release of nascent polypeptide chains by the addition of Puromycin dihydrochloride to the growing peptide chain. Protein synthesis is inhibited in both prokaryotic and eukaryotic cells that do not express the puromycin-N-acetyl-transferase (pac) gene encoding resistance. Puromycin dihydrochloride is as effective as Hygromycin B and G418, and selection can be achieved in 2-3 days.

Stock solutions may be prepared by dissolving the antibiotic in deionized water. When filter-sterilized (0.22 micron filter), stock solution may be stored at 4C for up to 1 year. The stock solution may also be divided into aliquots and stored frozen for greater stability.

The effective killing concentration of the antibiotic will vary as to cell type, media, growth conditions, density, and the cells metabolic rate and position in the cell cycle. When using puromycin dihydrochloride in a new cell system, a full dose curve is suggested, and with each new lot of puromcin dihydrochloride, several points on that curve should be retested.

Molecular Structure

Product SpecificationsMolecular Weight 544.4

Formula C22H29N7O5 * 2HCl

Mode of Action Aminoacyl tRNA mimic, causing premature polypeptide chain termination.

Conferred Resistance

Aminoglycoside-modifying enzymes, a change in cell permeability, or a change in ribosomal structure.

Spectrum Gram (+)Gram (-) bacilli aerobes and facultative anaerobes, only.

Purity 98%

Effective Concentration

0.5 to 10 g/mL

Appearance White to off-white powder

Storage Frozen (-15 to -25C), protected from light

Cat. No. Description Size Qty/Pk

61-385-RA Puromycin Dihydrochloride, powder 100 mg 1

Mediatech, Inc.A Corning Subsidiary

9345 Discovery Blvd. Manassas, VA 20109

t 800.235.5476f 703.471.0363

www.cellgro.com

For a complete listing of trademarks, visit us at www.cellgro.com/about-us/trademarks.html

Corning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001

cellgroThe Corning Family of Brands

Corning cellgro products are manufactured by Mediatech, Inc., a Corning Subsidiary