Arthropod Parasites of Veterinary importance

2015 VPM-122 Laboratory 6

Prepared by Spencer J. Greenwood PhD, DVM

Dept. of Biomedical Sciences Room 2332N AVC North Annex

sgreenwood@upei.ca Office phone: 566-6002

To see the images on the web, go to: http://people.upei.ca/sgreenwood/html/protozoa.html

VPM-122 Laboratory 6 Images

• The following images of been generated for your reviewing and studying pleasure…honest!

• I have typically provided two views of the same image – 1) so you can see diagnostic details – 2) a “boxed” image so you can see the relative size the

parasite looks on the specified microscope objective (e.g. 10X).

– Comments for improving this handout are welcome and encouraged!

– Thanks, Spencer

7) Leukocytozoon sp.

Leukocytozoon spp. from the blood of a duck. Elongate gametocyte within a leukocyte surrounded by many nucleated erythrocytes. Note: the ‘horns’ of faint blue cytoplasm extending beyond the cell nucleus.

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8) Babesia sp.

Babesia sp. from the blood of a cow. 100X objective (oil immersion), Note the mostly piriform (tear or pear) shaped merozoite/trophozoites within the erythrocytes. Wright-Giemsa stain.

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Demodex canis - adult mite from the skin of a dog.

1) Mites

Demodex sp. - adult mite from the skin of a dog.

Sarcoptes sabei var. canis - adult female mite from skin of a dog. Note: long unsegmented pedicles (stalks) with sucker at end on legs 1 & 2.

Sarcoptes sabei var. canis - adult mite from skin of a dog. Note: long unsegmented pedicle or stalk (black arrow) with sucker at end (red arrow).

Otodectes cyanotis - adult male mite from the ear of a dog. Note: short unsegmented pedicle with sucker at the end on each leg.

Otodectes mites male (top) and female (bottom). It is not unusual to see them under the microscope “joined together”...

Cheyletiella sp. - from the skin of a cat. A surface mite known as “Walking Dandruff”. Note: the long legs and the large palpal claws (arrow) on the ‘head’.

Cheyletiella sp. - Note: the large palpal claws (arrow)

Chorioptes sp. from a cow. Adult male. Note: the short unsegmented pedicles with suckers.

Psoroptes sp. from the a sheep. Note the long legs & long segmented pedicles (see below to convince yourself).

Knemidocoptes sp. found on a budgie. This is a burrowing mite known as the Scaly Face or Scaly Leg mite of birds. They have a Round body similar to Sarcoptes with small stubby legs. Females have no suckers. NOT ON LAB EXAM

2) TICKS

Ixodes sp. from a dog - Engorged female. Note: anal groove in front of anus.

Ixodes sp. from a deer - Note: arrow indicates the ‘anal groove in front of anus’ that is used to diagnose members of this Genus. Six legged larval stage.

Rhipicephalus sp. - A. Stained specimen with a noose!, Note the hexagonal shaped basis capitulum (red arrow) and deep clefts in the fore coxae (black arrow). B. Cleared specimen showing - hexagonal shaped basis capitulum

Dermacentor sp. from a dog. Note the ‘rectangular’ basis capitulum and festoons

Ctenocephalides felis - The cat flea. Pronotal comb (red arrow) and genal comb (black arrow).

Ctenocephalides felis - The cat flea. The most common flea on both cats and dogs

3) Fleas

4) Keds

Melophagus sp. - Adult sheep ked. Note how fly-like it looks. Often described as a wingless fly.

5) Lice

Trichodectes sp. found on a dog. This is a chewing louse Note: that the head is wider than the thorax. They have sx legs attached to the thorax. The front pair of legs are obscured by this louses massive head.

Linognathus sp. from the skin of a dog. Stained specimen (red dye)..

Hematopinus sp. from the skin of a pig.

Note: these are both sucking lice, the head is narrower than the thorax

6) Bots

A) Gasterophilus - Horse bot fly larvae (stomach of horse), ~ 1-1.5 cm in length B) Hypoderma - Cattle grub, back of cow, ~ 3cm in length C) Cuterebra - Rodent bot fly (from the neck skin of a dog), ~ 2.5 cm in length

A B C