Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Label Free Quantitative Proteomics

“biomarker discovery”

Label-free quantitative proteomics using qTOF MS

Sample,Tissue,Plasma Yeast

Enolase (100 fmol)

lyse

Individual proteins

lyse

Thousands ofproteins

Trypsin digest all proteins to peptides

Tens/ hundreds of thousands of peptides

QTOF

Sequenceeverything

Sample

Label-free quantitative proteomics using qTOF MS

Label-free quantitative proteomics using qTOF MS

Time

Amount of peptide(larger the response, more protein present)

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

UCL Institute of Child Health & Great Ormond Street Hospital for Sick Children UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Acknowledgements UCL