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Produzione bio-tecnologica di composti
bioattivi da matrici vegetali e studi di stabilità
per incapsulamento
Lecce
S.Caretto
Production of BIOACTIVE COMPOUNDS by plant cell cultures
Enhancement of product yields
Lecce
Encapsulation of BIOACTIVE COMPOUNDS obtained by differentplant sources
Improvement of product stability
Plant Cell Cultures: valuable tools forcontrolled production of Bioactive Compounds
Lecce
because of
the continuous supply of highly specialized natural compounds that cannot be
produced in equal quality or specificity by chemical synthesis
through
� the manipulation of culture conditions to lead to high
flux into metabolic pathways
� screening and selection of cell lines
� large scale growth in bioreactors
Plant tissue cultures for the production of bioactive compounds
and investigation on their metabolic pathways
Established Plant Cell Cultures
•Helianthus annuus
•Prunus sp.
•Eugenia myrtifolia
•Artemisia annua
Bioactive compounds
•Tocopherols
•Anthocyanins
•Artemisinin
Lecce
0
5
10
15
20
25
30
µµ µµg/g
fres
h w
eigh
t
HT LT
TocopherolChlorophyll
HT
Selection of sunflower cell lines with
enhanced vitamin E production
LT
Minutes 0,0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 25,0 27,5 30,0
mAU
0
200
400
600
800
1000
mAU
0
200
400
600
800
1000
10,133
11,300
13,133
13,967 12,200
B A
Cherry cell lines producing
anthocyanin
Tempo di trattamento
30 min 1ora 2 ore 4 ore 24 ore 48 ore 5gg
Art
emis
inin
a (µ
g/g
peso
sec
co)
0
4
8
12
16
0 5 10
mAU
0
50
100
150
200
0 5 10
mAU
0
50
100
150
200
MeJA elicited Artemisia cell
cultures
Main publications:
Caretto et al. (2004) Plant Cell Rep.; Gala et al. (2005) J. Plant Physiol.; Blando et al., (2005) Food Res. Int.
Fachechi et al. (2007) Plant Cell Rep.