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Production of proteins from clonedgenes
Terms:Expression vectorsFusion proteinsStrong/weak promotersGlycosylation patternCell-free translation
Cloning for protein produktion
Fig 13.1 p226
Production of eukaryotic proteins in E.coli
Fig 13.2 p227
Signals for gene expression
Fig 13.3 p228
Typical Promoter sequences
Fig 13.4 p228
Expression vector for expression of foreign genes in E.coli
Fig 13.5 p229
Strong/weak promoters
Fig 13.6 p230
Gene regulation
Fig 13.7 p230
Promoters in expression vectors
Fig 13.8 p231
Cassette vector
Fig 13.9 p232
Translation of fusion protein
Fig 13.10 p233
Problem with secondary structureat the start of transcription
Fig 13.11 p233
Affinity chromatografy for purification of Glutation-S-transferas
Fig 13.12 p234
Cleavage of a fusion protein
Fig 13.13 p234
Problem with expression of foreignproteins in E.coli
Fig 13.14 p235
Inclusion bodies
Fig 13.15 p236
Promoters for eukaryoticexpression vectors
Fig 13.16 p238
Glycosylation pattern
Fig 13.17 p239
Inclusion bodies in insect cells
Fig 13.18 p240
Cell free translation system
Cell-free translation
Figure 11.18p200