Producción Asparaginasa

7/24/2019 Produccin Asparaginasa

1/21

PONTIFICIA UNIVERSIDAD CATLICA DE VALPARAISO

FACULTAD DE INGENIERA

ESCUELA DE INGENIERA BIOQUIMICA

ICB 540

PRODUCCION DE METABOLITOS

ASPARAGINASA

Alumnos P!m"l! Co#$"s

Cl!u%&! M!ll'!

N!(!%"$ Ro%#')u"*

P#o+"so# Cl!u%&! Al$!m!no

,ul&o B"##&os

A-#&l .0/5

Introducc

in

10% 70

Disc.

Bibliog,

50% 60

Conclusi

n

30% 65

Referenci 10% 50


2/21

INDICE

CAPITULO /

INTRODUCCIN

CAPITULO .

./ RELEVANCIA 1 APLICACIN

.. PRO1ECCIONES DE LA PRODUCCION

.2 PROCESO DE PRODUCCION

CAPITULO 2

CONCLUSIONES

REFERENCIAS

BIBLIOGRAFIA

ANE3OS

ANE3O /

MECANISMO DE ACCION ASPARAGINASA

ANE3O .

DIFERENTES FUENTES BACTERIANAS PARA ASPARAGINASA

ANE3O 2

EVOLUCION TEMPORAL DE LA APARACION DE ASPARAGINASA II

ANE3O 4

USO INDUSTRIAL DE ACR1LAA1 EN EL MUNDO


3/21

INDICE DE TABLAS

T!-l! / D&+"#"n$"s Fu"n$"s -!6$"#&!n!s 7!#! !s7!#!)&n!s!

INDICE DE FIGURAS

F&)u#! / Es8u"m! m"6!n&smo %" #"!66&9n !s7!#!)&n!s!

F&)u#! . E:olu6&9n $"m7o#!l %" l! !7!#&6&9n %" !s7!#!)&n!s! II "n 6ul$&:os

F&)u#! 2 Uso &n%us$#&!l %" No:o*(m"s A6#(l!;!( "n "l mun%o !l !


4/21

4

CAPITULO /

INTRODUCCIN

L! !s7!#!)&n!s! "s l! "n*&m! 8u" %"s!m&%! l! !s7!#!)&n! =!m&no>6&%o no "s"n6&!l?@

&%#ol&*>n%ol! !s$! >6&%o !s7>#$&6o ( !mon&o Es$! "n*&m! s" "n6u"n$#! 7#"s"n$" $!n$o

"n 7l!n$!s 6omo "n m!m'+"#os ( "s$> %&s$#&-u&%! !m7l&!m"n$" "n m&6#oo#)!n&smos@ 6omo

-!6$"#&!s@ on)os ( l":!%u#!s =Im!%! "$ !l@ /2? Aun 6u!n%o l! !s7!#!)&n!s! no $&"n"

un #ol 6"n$#!l "n "l m"$!-ol&smo@ "s &m7o#$!n$" s"

l&n+o-l>s$&6! !)u%! En un )#!n nm"#o %" 6!sos@ "l $#!$!m&"n$o 6on !s7!#!)&n!s! 7"#m&$"

un! "l&m&n!6&9n 6om7l"$! %" l! "n+"#m"%!%@ 6!$!lo)!n%o l! "n*&m! 6omo un! %#o)!

-&o8u'm&6! "+"6$&:! =G!ll!)"# "$ !l@ /?

No o-s$!n$" l! !%m&n&s$#!6&9n %" !s7!#!)&n!s! %" o#&)"n -!6$"#&!no 7u"%" 6!us!#

&7"#s"ns&-&l&%!% ! l!#)o 7l!*o@ %!n%o lu)!# ! #"!66&on"s !l#)&6!s ( !n!+&l!&!@ 7o# lo 8u"

7!#! %&sm&nu "s$os "+"6$os s" ! &n$"n$!%o "m7l"!# "n*&m!s %" %&+"#"n$"s +u"n$"s

-&ol9)&6!s@ 7on&"n%o !l %"s6u-&"#$o &m7o#$!n$"s !s7"6$os %" l! #")ul!6&9n@ +un6&9n@

"s$#u6$u#! ( m"6!n&smos 6!$!l'$&6os =R"(nol%s !n% T!(lo#@ /2?

L! 7#o7&"%!% !n$&$umo#!l %" l! !s7!#!)&n!s! s" %"s6u-#&9 o#&)&n!lm"n$" "n "l su"#o %"

6o-!(o /52@ 7"#o "l uso %" "s$" $#!$!m&"n$o s" %"s"s$&m9 7o# su !l$o 6os$o %"

7#o%u66&9n A6$u!lm"n$" ! s&%o %"#&:!%! %" un! )#!n :!#&"%!% %" +u"n$"s -!6$"#&!n!s

7"#o los n&6os %os 7#o%u6$os 8u" 7#"s"n$!n un "$"nso uso "n $#!$!m&"n$os 6l'n&6os son

!8u"ll!s "n*&m!s %"#&:!%!s %" E. coli( Erwinia=P!#H 1 "$ !l@ //?


5/21

5

CAPITULO .

./ RELEVANCIA 1 APLICACIN

L! !s7!#!)&n!s! "s un! "n*&m! 8u" &%#ol&*! l! !s7!#!)&n! "n !mon&o ( >6&%o !s7>#$&6o@

( l! )lu$!m&n! "n >6&%o )lu$>m&6o ( !mon&o =S6#"( "$ !l@ .0//?@ "l "s8u"m! s" 7u"%"

o-s"#:!# "n l! F&)u#! / %"l:!s"!n"o / Aun8u" s!-"mos 8u" l! +un6&9n "s s&m7l" ( l!

!s7!#!)&n! "s un !m&no>6&%o no "s"n6&!l@ $&"n" #"7"#6us&9n &m7o#$!n$" "n "l $#!$!m&"n$o

%" l! l"u6"m&!@ s&"n%o 6ons&%"#!%! 6omo un "l"m"n$o 6"n$#!l "n "l $#!$!m&"n$o %" l!

l"u6"m&! l&n+o-l>s$&6! !)u%! =LLA? 7"%&>$#&6! =S6#"( "$ !l@ .0// M"##(m!n "$ !l@ .0/.

!n) "$ !l@ .002?@ !%"m>s 7u"%" u$&l&*!#s" 6omo "s8u"m!s %" $"#!7&! "n l&n+om!s no

Jo%)H&n o l"u6"m&! m&"lo-l>s$&6! !)u%! =E)u&)u#"n "$ !l@ /.?

L! !s7!#!)&n!s! !6$! 6omo !)"n$" !n$&n"o7l>s&6o@ "s %"6 "n 6lul!s 8u" 7#"s"n$!n

un! !l$"#!6&9n %" l! 7#ol&+"#!6&9n (@ mu6!s :"6"s@ %" l! m&$os&s L!s 6lul!s n"o7l>s&6!s

no 7u"%"n s&n$"$&*!# !s7!#!)&n!@ %"-&%o ! l! !us"n6&! %" !s7!#!)&n! s&n$"$!s! =K"!$&n) "$

!l@ /2?@ 7o# lo 6u!l !l no s"# 6!7!6"s %" 7#o%u6 "s$" !m&no>6&%o@ %"7"n%"n

7#&n6&7!lm"n$" %" l! !s7!#!)&n! %"l 7l!sm! 6un%!n$" Con "s$! #"+"#"n6&! 7o%"mos

no$!# un! %" l!s 7o6!s %&+"#"n6&!s "s7"6'+&6!s 8u" 7o%"mos #"6ono6"# "n$#" 6lul!s

no#m!l"s ( 6!n6"#os!s En$on6"s 6omo l! m!(o#'! %" l!s 6lul!s no#m!l"s s&n$"$&*!n

!s7!#!)&n!@ no son !+"6$!%!s 7o# un! %"+&6&"n6&! &n%u6&%! %" "s$! sus$!n6&!

L! &%#9l&s&s %" l! !s7!#!)&n! #"sul$! "n un !)o$!m&"n$o 6on$&nuo@ 7o# "s$! #!*9n@ l!

7#>6$&6! $"#!7u$&6! m>s 6omn 6ons&s$" "n &n("6$!# 7o# :'! &n$#!:"nos! "n*&m! l&-#" 6on

"l +&n %" %&sm&nu l! 6on6"n$#!6&9n "n s!n)#" %" !s7!#!)&n! ( !s' !+"6$!# s"l"6$&:!m"n$"

! l!s 6lul!s n"o7l>s&6!s =M&$6"ll "$ !l@ /4?@ 7o# "s$o 7o%"mos %"6 8u" -!o

%"$"#m&n!%o "n$o#no l!s 6lul!s 6!n6"#os!s no $&"n"n 7os&-&l&%!% %" so-#":&:@ ( 6on "l

$&"m7o "s$o 6onll":! ! un! #"m&s&9n %"l 6>n6"#

S&n "m-!#)o %u#!n$" "l $#!$!m&"n$o l! m!(o#'! %" los 7!6&"n$"s 7#"s"n$!n #"!66&on"s %"

&7"#s"ns&-&l&%!% ! l! !s7!#!)&n!s! %" o#&)"n -!6$"#&!no@ u$&l&*!%o ! l!#)o 7l!*o =R"(nol%s

!n% T!(lo#@ /2? Po# 6ons&)u&"n$" nos 7!#"6" 7#&mo#%&!l l! -s8u"%! %" o$#!s +u"n$"s %"


6/21

6

!s7!#!)&n!s!@ 6omo m&6#oo#)!n&smos "u6!#&o$!s@ 8u" 7u"%"n 6on%u6 ! un! "n*&m! 6on

m"nos "+"6$os !%:"#sos

L! !s7!#!)&n!s! "n E. Coli%"mos$#9 8u" $&"n" !6$&:&%!% !n$&$umo#!l ( 8u" su 7#o%u66&9nm"%&!n$" s&s$"m!s m&6#o-&!nos ! !$#!'%o l! !$"n6&9n %"-&%o ! su n!$u#!l"*! #"n$!-l" (

"6ol9)&6! =B#oom"@ // M!s-u#n !n% #&s$on@ /4? Cons&%"#!mos 8u" l! l"u6"m&!

"s un! "n+"#m"%!% %" )#!n &n6&%"n6&! !6$u!lm"n$"@ 7o# lo 8u" l! 6o-"#$u#! 7!#! "s$" $&7o

%" $#!$!m&"n$o %"-&"s" s"# m>s !m7l&! Es$o %"-&%o ! 8u" !( #")&on"s %"l mun%o 8u" no

$&"n"n !66"so ! %&6os $#!$!m&"n$os ( s& s" 8u&"#"n &m7l"m"n$!# "s 6on:"n&"n$"@ %"s%" "l

7un$o %" :&s$! "6on9m&6o@ u$&l&*!# !s7!#!)&n!s! 7#o%u6&%! 7o# E. Coli@ 7!#! ":&$!# 6os$os

&n:olu6#!%os "n ll":!# ! 6!-o un! nu":! &n:"s$&)!6&9n ( "s$u%&os 6l'n&6os

L! !s7!#!)&n!s! "s !%"m>s u$&l&*!%! "n o$#!s !#"!s 6omo l! &n%us$#&! %" los !l&m"n$os

L! !6#&l!m&%! "s un! !m&%! &ns!$u#!%! !l$!m"n$" #"!6$&:!@ ! l! 6u!l s" l" !so6&! un

6ons&%"#!-l" #&"s)o %" 6>n6"# En "l .00. s" %"mos$#9 8u" l! +o#m!6&9n %" "s$"

mon9m"#o s" #"!l&*! ! $"m7"#!$u#!s su7"#&o#"s ! los /.0C "n %&+"#"n$"s !l&m"n$os

%"#&:!%os %" los 6"#"!l"s 6omo $!m-&n "n 7!7!s 8u" 6on$&"n" !l$os n&:"l"s %"

6!#-o&%#!$os@ %>n%os" lu)!# l! #"!66&9n "n$#" !s7!#!)&n! ( !*u6!#"s #"%u6$o#"s

m"%&!n$" l! #"!66&9n %" M!&ll!#% =T!#"H" "$ !l@ .00. C!7u!no .00?

D"-&%o ! "llo s" )"n"#9 un! 7#"o6u7!6&9n mun%&!l so-#" sus 7os&-l"s #&"s)os 7!#! l!

s!lu% Du#!n$" .00 ! .00 l! 6om&s&9n Eu#o7"! #"6om"n%9 ! los "s$!%os m&"m-#os

mon&$o#"!# los n&:"l"s %" !6#&l!m&%! "n los !l&m"n$os ( "n .0// "n$#")9 un! s"#&" %"

#"6om"n%!6&on"s 7!#! #"%u6 los n&:"l"s %" "s$" 6om7u"s$o =E6 =Eu#o7"!n Com&s&on?@

.0//?

Son num"#osos los !#$'6ulos 8u" 7#omu":"n l!s !s7!#!)&n!s!s %" %&+"#"n$"s o#')"n"s

6omo m"%&o 7!#! ":&$!# l! +o#m!6&9n %" !6#&l!m&%! ll":!n%o l! !s7!#!)&n! !6&! >6&%o

!s7>#$&6o ( ":&$!n%o !s' 8u" o6u##! l! 7os$"#&o# #"!66&9n 6on 6!#-o&%#!$os =Ku&lm!n !n%

&lms@ .00? As' s" 7u"%" :"# su uso "n 7#o%u6$os o#n"!%os =D" Bo"# "$ !l@ .0/4?@


7/21

7

$!m-&n "n 7!7!s +#!n6"s!s =P"%#"s6& "$ !l@ .00? ( "n )!ll"$!s =An"s" "$ !l@ .0//?

P"ns!mos 8u" "s$o %"mu"s$#! 8u" l! !s7!#!)&n!s! "s un m$o%o "+"6$&:o "n l!

#"%u66&9n %" !6#&l!m&%!@ 7!#"6&"n%o s"# l! "s$#!$")&! m>s "+&6!* "n 7#o%u6$os %"

6!#!6$"#'s$&6!s m"n6&on!%!s !n$"#&o#m"n$" 7u"s$o 8u" s" !7l&6! !n$"s %" l! "7os&6&9n !

l!s !l$!s $"m7"#!$u#!s ( #"%u6&"n%o "n )#!n 6!n$&%!% l! !6#&l!m&%!@ ":&$!n%o !s' 6!m-&os%" 7J o $"m7"#!$u#! %u#!n$" l! 7#"7!#!6&9n@8u" :!n "n %"sm"%#o %" l!s 7#o7&"%!%"s

o#)!nol7$&6!s %"l !l&m"n$o

Po# o$#o l!%o s" !n 7#o7u"s$o usos &n)"n&osos ! l! !s7!#!)&n!s! @6omo "n "l 6!m7o %"

l! "$#!66&9n 7"$#ol"#! L!s 7ol&!6#&l!m&%!s son u$&l&*!%!s 6omo !%&$&:os "n mu6!s "$!7!s

%"l 7#o6"so %" 7"#+o#!6&9n@ 7#o%u66&9n@ $#!ns7o#$" ( 7#o6"s!m&"n$o %" 6#u%o s&"n%o mu(

"+"6$&:!s "n su !7l&6!6&9n ( 6on 6!#!6$"#'s$&6!s %"s"!-l"s 7!#! l!s %&+"#"n$"s $!#"!s@ "n"s7"6&!l "n #"6u7"#!6&9n m"o#!%! %" 6#u%o ( 6omo +lu&%o %" 7"#+o#!6&9n S&n "m-!#)o

lu")o %" u$&l&*!%!s@ l! :&s6os&%!%@ 8u" "s un! %" l!s 6!#!6$"#'s$&6!s 7#&n6&7!l"s %" "s$os

7ol'm"#os@ 6om&"n*! ! s"# un 7#o-l"m!@ 7P!#! "llo s" ! 7#o7u"s$o "l uso %"

!s7!#!)&n!s! l! 6u!l 7u"%" %")#!%!# l!s 7ol&!6#&l!m&%!s #"%u6&"n%o l! :&s6os&%!% "n

+lu&%os 8u" l!s 7os"!n@ "n "s7"6&!l l! 7#o:"n&"n$" %"Aspergillus oryzae=Gu7$!@ .0/.?

.. PRO1ECCIONES DE LA PRODUCCION

L! -s8u"%! %" nu":os m&6#oo#)!n&smos 8u" 7u"%!n s&n$"$&*!# "s$! "n*&m! ( 6on

m"no#"s "+"6$os !%:"#sos 7o%#> s"# %" !(u%! "n "l $#!$!m&"n$o %"l 6>n6"# =D" Mou#!

S!#8u&s "$ !l@ .004? D"-&%o ! 8u" l!s #"!66&on"s %" &7"#s"ns&-&l&%!%@ s" 6ons&%"#!n

6omo un ":"n$o $9&6o %" m!(o# +#"6u"n6&!@ s&"n%o m"no#"s "s$!s #"!66&on"s 6on l!

!s7!#!)&n!s! 7#o:"n&"n$" %" Erwinia=!n) "$ !l@ .002 S6"#"( "$ !l@ .0//? L! n&6!

m!n"#! %" 6on$&nu!# 6on "s$" "s8u"m! %" $"#!7&! "s "l 6!m-&o %" !s7!#!)&n!s!

7#o%u6&%! %" E. coli ! l! 7#o%u6&%! 7o# o$#os m&6#oo#)!n&smos 6omo Erwinia@ o

6om-&n!#l!s "n "l $#!$!m&"n$o


8/21

En 6u!n$o ! l! &n%us$#&! %" los !l&m"n$os@ Cono6"mos 8u" l! &n%us$#&! %" los

!l&m"n$os"s$!s" "s$> !-#&"n%o 7!so !6&! 7#o%u6$os m>s s!lu%!-l"s ( +un6&on!l"s L!

!6#&l!m&%!@ 6om7u"s$o $9&6o@ s" )"n"#! "n %&+"#"n$"s !l&m"n$os mu( 7o7ul!#"s 6omo "l

6!+@ "n 7!'s"s %"s!##oll!%os solo "l 6onsumo %" "s$" o$o#)! "l .5 %" l! !6#&l!m&%!

6onsum&%! $o$!l =P!:"ss&@ .0/4? L! !s7!#!)&n!s! s" u$&l&*! %" mo%o "+&6!* %"s%" .006on l! !7!#&6&9n %" A6#(l!;!( ( o$#!s !s7!#!)&n!s!s 6om"#6&!l"s@ 7o%"mos %!# 6u"n$!

8u" m&"n$#!s s&)! 6#"6&"n%o "l m"#6!%o !l&m"n$!#&o %" 7#o%u6$os o#n"!%os su %"m!n%!

!um"n$!#> L! solu6&9n "s !6$u!lm"n$" u$&l&*!%! "n m>s %" 20 7!'s"s "n un! !m7l&!

)!m! %" 7#o%u6$os C#""mos 8u" "s$o %"mu"s$#! "l !l*! 8u" ! $"n&%o %"s%" su

!7!#&6&9n ( l! &m7o#$!n6&! %" l! !s7!#!)&n!s!@ 7#&n6&7!lm"n$" %"s%" 8u" l! EFSA ( l! FDA

#"6om"n%!#on ! l! &n%us$#&! !l&m"n$!#&! s"# 6ons6&"n$"s %" los n&:"l"s %" !6#&l!m&%! "n

sus 7#o%u6$os ( l! !7l&6!6&9n %" nu":!s $"6nolo)'!s 7!#! #"%u6los =FDA@ .0/2?

.2 PROCESO DE PRODUCCION

En /52 s" o-s"#:9 8u" "l su"#o %" 6o-!(o 6!us9 #")#"s&9n %" !l)unos $&7os %" $umo#"s

"n #!$on"s ( #!$!s O6o !s $!#%"@ "l !)"n$" !n$&$umo#!l "n "l su"#o %" 6o-!(! +u"

&%"n$&+&6!%o 6omo !s7!#!)&n!s! =B#oom"@ //?@ s&"n%o s$" "l 7#&m"# 7#o6"so %"

o-$"n6&9n %" !s7!#!)&n!s! 7!#! "l $#!$!m&"n$o %" $umo#"s El !n>l&s&s #":"l9 8u" un!

un&%!% In$"#n!6&on!l =UI? %" !s7!#!)&n!s! "s l! 6!n$&%!% 8u" :! ! l&-"#!# un m&6#omol %"

NJ %" !s7!#!)&n! L! %os&s um!n!@ 7u"%" #"8u"# /000000 UI o m>s 7o# %'! 7o#

7"#son!@ s&)n&+&6!n%o 8u" 7!#! l! 7#o%u66&9n %" $!l"s %os&s s"#'! n"6"s!#&o "$#!"# "l

su"#o %" m>s %" 20000 6o-!(os 7o# %'! 7o# 7"#son! =Ro-"#$s !n% J&ll@ /? C#""mos

8u" "l 6os$o 8u" )"n"#!-! "l $#!$!m&"n$o 7o# 7"#son!@ 6ons&%"#!n%o $!n$o l! 6!n$&%!% %"

!n&m!l"s %" los 8u" s" %"-'! "$#!"# "l su"#o ( "l 6os$o %" 7#o6"s!# "l m&smo 7!#! un!

%os&s %&!#&!@ "#! "6"s&:!m"n$" !l$o@ 7o# lo $!n$o "s$" m$o%o no "#! #"n$!-l"

Du#!n$" los s&)u&"n$"s !


9/21

!

u$&l&*!%!s (P!#H "$ !l@1981) L!s 7#"7!#!6&on"s 6om"#6&!l"s %" !s7!#!)&n!s! m>s us!%!s

o( "n %'! son 6&%"#ol!s"@ "ls7!#@ m"%!6@ on6!s7!#@ "#8&n!s" =O#$un@ .0/.?

L! 7#o%u66&9n %" !s7!#!)&n!s! II 7o# E. colis" ll":! ! 6!-o "n 6ul$&:os 6on$&nuos "n un

m"%&o %" +"#m"n$!6&9n %u#!n$" l! $#!ns&6&9n %" 6#"6&m&"n$o !"#9-&6o ! !n!"#9-&6o =C"%!#

!n% S6;!#$*@ /? El 6u#so $"m7o#!l %" l! !7!#&6&9n %" l! "n*&m! -!o "s$!s

6on%&6&on"s s" mu"s$#! "n "l !n"o .2 Lu")o l!s 6lul!s %" E. colison #"6o)&%!s %"l

6ul$&:o 7!#! o-$"n"# un "$#!6$o !6uoso 8u" 6on$&"n" !s7!#!)&n!s!s@ 7u#&+&6!n%o (

6on6"n$#!n%o "l "$#!6$o 7o# 7#"6&7&$!6&9n +#!66&on!%! ( 6#om!$o)#!+'! To%o "s$o s"

#"!l&*! "n un m"%&o %" +"#m"n$!6&9n 8u" 6on$&"n" un! +u"n$" %" nu$#&"n$"s !s&m&l!-l" %"

6!#-ono@ ( 7!#! 7#o:""# %" un! +u"n$" %" nu$#&"n$" %" n&$#9)"no s" u$&l&*! un! 7"7$on!

7#"+"#&-l"m"n$" 7#o%u6&%! 7o# l! %&)"s$&9n 7!n6#">$&6! %" l! 6!s"'n!@ l! 6u!l s" :"n%"

-!o l! m!#6! 6om"#6&!l N !m&n! T&7o B =M!s-u#n !n% #&s$on@ /4?

Po# o$#o l!%o l! 7#o%u66&9n %" !s7!#!)&n!s!s %" Erwinias" 7#"7!#! ! )#!n "s6!l! "n

6ul$&:os 7o# lo$" ( "n un! 6olumn! %" 6#om!$o)#!+'! %" &n$"#6!m-&o &9n&6o S" #"8u&"#" un

)#!n nm"#o %" "$!7!s 7!#! !l6!n*!# l! 7u#"*! n"6"s!#&!@ 6on 6#om!$o)#!+'! %"

&n$"#6!m-&o &9n&6o 7o# lo$" L! 7#>6$&6! 6om"#6&!l !6$u!l &m7l&6! "l 6#"6&m&"n$o %" un6ul$&:o %" 6lul!s@ ( lu")o un! "$#!66&9n %" 7#o$"'n! 6#u%! "m7l"!n%o &n$"##u76&9n

!l6!l&n! ( 7#"6&7&$!6&9n 6on >6&%o El "$#!6$o #"sul$!n$" s" som"$" "n$on6"s ! l!

!%so#6&9n ( "lu6&9n %" CM6"lulos! Lu")o s&)u" un! "$!7! %" 7#"6&7&$!6&9n %" >6&%o m>s

( "$!7!s %" 6#om!$o)#!+'! u$&l&*!n%o CM ( m"%&os DEAE6"lulos! =Go;!#%@ /4?

Po%"mos !7#"6&!# 8u" l! s")u&%&ll! %" "$!7!s !6" 8u" s"! un 7#o6"so 6on un! m!no %"

o-#! &n$"ns! 7!#! l! o-$"n6&9n 7u#! %" !s7!#!)&n!s!@ #"8u&n%os" %" 6u&%!%os (

7#"6&s&on"s 7!#! 8u" s"! un 7#o6"so +&!-l" ( +!6$&-l" A%"m>s 6#""mos 8u" l!s 7#>6$&6!s

8u" s" #"!l&*!n "n "l 7#"s"n$" son 7!#! o-$"n"# m!(o#"s 6!n$&%!%"s %" !s7!#!)&n!s! !7!#$ %" %"$"#m&n!%!s 6!n$&%!%"s %" m&6#oo#)!n&smos@ #"%u6&n%os" !s' "l )!s$o %"

7#o%u6 l! "n*&m!@ !6&"n%o "6on9m&6!m"n$" m>s +!6$&-l" l! !%m&n&s$#!6&9n "n

7!6&"n$"s Po# o$#! 7!#$" 7"ns!mos 8u" "s %" 6ons&%"#!-l" &m7o#$!n6&! 6om"#6&!l 8u"

"&s$! !6$u!lm"n$" l! 7#o%u66&9n %" !s7!#!)&n!s! 7o# %os m&6#oo#)!n&smos %&+"#"n$"s@


10/21

10

7"#m&$&"n%o ! 7!6&"n$"s 8u" 7#"s"n$"n "+"6$os s"6un%!#&os 6on un $#!$!m&"n$o@ un!

$"#!7&! !l$"#n!$&:!

Un! "!m&n!6&9n 6om7!#!$&:! %" E. coli!s7!#!)&n!s! 7#o%u6&%! "n USSR@ Al"m!n&! (,!79n +u" "6! 7o# =Kon%#!$@ /4? ( #":"l9 8u" l!s 6!#!6$"#'s$&6!s 6l'n&6!s %" l!

7#"7!#!6&9n "6! "n URSS ( Al"m!n&! "#!n &%n$&6os L! !66&9n !n$& l"u6m&6! %" l!

7#"7!#!6&9n "6! "n l! URSS +u" su7"#&o# 8u" ! l! "6! "n ,!79n Po# lo $!n$o Ss"

#"6om&"n%! "l uso 6l'n&6o %" !s7!#!)&n!s! "6! "n URSS ( Al"m!n&! =V"#m! "$ !l@

.00?

Un! +o#m! &nno:!%o#! %" "l&m&n!# "l 6>n6"# 8u" 6!us! l! !6#&l!m&%! %" 7!n ! s&%o%"s!##oll!%! 7o# &n:"s$&)!%o#"s %" l! "m7#"s! 8u'm&6! ol!n%"s! DSM@ u$&l&*!n%o

$"6nolo)'! %" )"n"s 7!#! %")#!%!# l! !s7!#!)&n!@ "l !m&no>6&%o l&-#" 7#"6u#so# %" l!

!6#&l!m&%!@ !n$"s %" l! 6o66&9n Es$" 7#o6"so &m7l&6! l! 6lon!6&9n %" un! 6"7! %"

!s7!#!)&n! &%#ol&*!%! 7o# !s7!#!)&n!s! %"s%" Aspergillus Nigerniger L! 6"7!

#"sul$!n$" %" l! +"#m"n$!6&9n s" 7u#&+&6! !n$"s %" s"# !

( ! un 7J %" "n$#" 0 ! 0 D!%o 8u" l!s $"m7"#!$u#!s %" 6o66&9n ! m"nu%o su-"n !

/.0 C@ "s 6on:"n&"n$" %&s7on"# %" "n*&m!s 8u" s"!n "s$!-l"s ( !6$&:!s "n un! !m7l&!

)!m! %" $"m7"#!$u#! ( 7J =S&n! "$ !l@ .0/2? En "l !s %" 20 7!'s"s &n6lu&%o C&l" s")n %!$os %" l! m&sm! "m7#"s! =:!s" !n"o 4?


11/21

11

Aun8u" ! $#!ns6u##&%o 7o6o $&"m7o %"s%" 8u" s" $u:o 6ons$!n6&! %" "s$! :'! %"

6on$!m&n!6&9n 7o# !6#&l!m&%! "n um!nos@ ! $#!:s %" !l&m"n$os 8u" &n)"#&mos %&!#&os@ "l

!:!n6" 6&"n$'+&6o ! s&%o mu( #>7&%o "n "l %"s!##ollo %" m$o%os +!6$&-l"s ! l! o#! %"

%&sm&nu ( "l&m&n!# 6!n$&%!%"s %" !6#&l!m&%! 7!#! #"%u6 los #&"s)os %" s!lu% 8u"

7u"%"n !+"6$!#nos

F!l$!n %!$os %" :"n$!s !nu!l"s =-us8u"n "n 7!)&n!s %" "m7#"s!s o "n !%u!n!@ (! 8u"

s!-"n 8u" "n 6&l" $- l! us!n?

CAPITULO 2

CONCLUSIONES

S& -&"n l! !s7!#!)&n!s! no 7os"" un! !m7l&! :!#&"%!% %" !7l&6!6&on"s@ "s$o no 8u&"#"

%"6 8u" no s"! &m7o#$!n$" R"s7"6$o !l uso $"#!7u$&6o 6on$#! l! l"u6"m&! $o%!:'! !(

un l!#)o 6!m&no 7o# #"6o##"# S&n "m-!#)o !6$u!lm"n$" "s un! %" l!s !l$"#n!$&:!s m>s

"+"6$&:!s 6on$#! l! l"u6"m&! l&n+o-l>s$&6! !)u%! ( un! "s$#!$")&! !l$!m"n$" #"n$!-l" 6on$#!

l! !6#&l!m&%! "n !l&m"n$os@ l" ! %!%o #"l":!n6&! ! "s$! "n*&m! 8u" 7o%#'! "n6on$#!#

nu":!s !7l&6!6&on"s@ 6omo ! %"mos$#!%o l! 7!$"n$" #"+"#"n$" ! su uso "n l! &n%us$#&!

7"$#ol"#!

S& o$#!s !7l&6!6&on"s@ 6omo l! m"n6&on!%! "n 7o*os 7"$#ol'+"#os@ 6om&"n*!n ! s"#

u$&l&*!%!s s" 7o%#'! :"# un !um"n$o "n l! %"m!n%! %" l! !s7!#!)&n!s! A%"m>s

!7l&6!6&9n %" !s7!#!)&n!s! "n l! &n%us$#&! !l&m"n$!#&! 7!#! l! "l&m&n!6&9n %" l! 6!us!

6>n6"# %" !6#&l!m&%! %" los !l&m"n$os !l o#no ! s&%o uno %" los %"s6u-#&m&"n$os

"m&n"n$"s %" l! 7o6! mo%"#n! Po# lo $!n$o s" n"6"s&$! mu6o m>s $&"m7o 7!#!

"7lo#!# !6"#6! los 7os&-l"s usos %" "s$! &n6#"'-l" "n*&m!


12/21

1"

REFERENCIAS

/ An"s"@ M B@ Qu!#$!@ ,@ F#&!s .0// Mo%"ll&n) $" "++"6$ o+ !s7!#!)&n!s" &n

#"%u6&n) !6#(l!m&%" +o#m!$&on &n -&s6u&$s Foo% C"m&s$#(@ /. @ 77 425440

. B#oom"@ , D // E:&%"n6" $!$ $" L!s7!#!)&n!s" !6$&:&$( o+ )u&n"! 7&) s"#um

&s #"s7ons&-l" +o# &$s !n$&l(m7om! "++"6$s N!$u#" /////4 ///5 2

2 C!7u!no@ E A@ F"##&)no@ I A6!m7!@ A S"#7"n@ W AX!#@ V GYHm"n@ "$ !l .00

E++"6$ o+ +lou# $(7" on M!&ll!#% #"!6$&on !n% !6#(l!m&%" +o#m!$&on %u#&n) $o!s$&n) o+-#"!% 6#&s7 mo%"l s(s$"ms !n% m&$&)!$&on s$#!$")&"s Foo% R"s"!#6 In$"#n!$&on!l@

4. @ 77 /.5/20.

4 C"%!#@ J@ , S6;!#$* / P#o%u6$&on o+ LAs7!#!)&n!s" II -(

Es6"#&6&! 6ol& , B!6$"#&ol .042.04

5 D" Bo"#@ L CEM J""#"m!ns@ RB M"&m! .0/4 R"%u6$&on o+ !6#(l!m&%"

+o#m!$&on &n -!H"#( 7#o%u6$s -( !77l&6!$&on o+As7"#)&llus N&)"# !s7!#!)&n!s" In

SP C!u:!&n@ LS 1oun) !n% S S!lmon Us&n) C"#"!l S6&"n6" !n% T"6nolo)( +o#

$" B"n"+&$ o+ Consum"#s@ 7740/405 UH oo%"!% Pu-l&s&n)


13/21

13

D" Mou#!@ M E@ Mo#!&s A@ S&l:! G@ L!#! .004 P#o%u6$&on o+ L!s7!#!)&n!s" -(

F&l!m"n$ous Fun)& M"mo#&!s Do Ins$&$u$o Os;!l%o C#u* =5? 44.

Dunn@ , R"s"!#6 &n -#&"+ ,un" /@ .004 =6&$"% .. A7#&l .0/5? A:!&l!-l" +#om

Z;;;+oo%m!nu+!6$u#"6ouH[n";s[+ulls$o#(77[!&%[/[R"s"!#6 &n -#&"+$ml\

E)u&)u#"n ,M@ Pu& CJ Bon" M!##o; n"6#os&s !n% $#om-o$&6 6om7l&6!$&ons &n

6&l%oo% !6u$" l(m7o-l!s$&6 l"uH"m&! M"% P"%&!$# On6ol /..050

G!ll!)"# M P@ M!#s!ll R D ( &lson R =/? As7!#!)&n!s" !s ! %#u) +o#$#"!$m"n$ o+ !6u$" l(m7o-l!s$&6 l"uH!"m&! Ess!(s &n -&o6"m&s$#( .4 /40

/0 Gu7$!@ D S =.0/.? P!$"n$" n US.0/.0.55 A/ US

// Go;!#% C =/4? Patente n US 52/00 A US

/. Im!%! A@ I)!#!s& S@ N!H!!m! K " Isono M =/2? As7!#!)&n!s" !n% )lu$!m&n!s"

!6$&:&$&"s o+ m&6#oo#)!n&sms , G"n M&6#o-&ol 5

/2 Ku&lm!n@ M L@ &lms .00 S!+"$( o+ $" "n*(m" !s7!#!)&n!s"@ ! m"!ns o+

#"%u6$&on o+ !6#(l!m&%" &n +oo% To&6olo)( L"$$"#s .25 S/S/ Els":&"#

/4 K"!$&n) M,@ Jolm"s R@ L"#n"# S@ Jo DJ /2 L!s7!#!)&n!s" !n% PEG

!s7!#!)&n!s" P!s$@ 7#"s"n$ !n% +u$u#"L"uH L(m7om! /0 /52/5


14/21

14

/5 Kon%#!$@ E /4 Com7!#!$&:" ":!lu!$&on o+ $" 6l&n&6!l "++&6!6( o+ 2 7#"7!#!$&ons

o+ L!s7!#!)&n!s" +#om Es"#&6&! 6ol& An$&-&o$&H&=Mos6o;?.=? 5.52/

/ M!s-u#n@ L T@ !n% , C #&s$on /4 Tumo# &n&-&$&on "++"6$ o+ L!s7!#!)&n!s"+#om Es6"#&6&! 6ol& A#6 B&o6"m B&o7(s /05450 45.

/ M"##(m!n R@ S$":"nson KE@ Gos$&6 ,@ N"u-"#) D@ O]B#&!n ,@ S!ll!n SE@ "$ !l

As7!#!)&n!s" !sso6&!$"% m("losu77#"ss&on !n% "++"6$ on %os&n) o+ o$"#

6"mo$"#!7"u$&6 !)"n$s &n 6&l%oo% !6u$" l(m7o-l!s$&6 l"uH"m&! P"%&!$#&6

Bloo% C!n6"# .0/.5.5

/ M&$6"ll I@ Joo)"n%oo#n J@ G&l"s AR /4 In6#"!s"% "n%o)"nous $#om-&n

)"n"#!$&on &n 6&l%#"n ;&$ !6u$" l(m7o-l!s$&6 l"uH"m&! #&sH o+ $#om-o$&6

6om7l&6!$&ons &n L !s7!#!)&n!s" &n%u6"% !n$&$#om-&n III %"+&6&"n6(Bloo% 2 2

2/

/ Mo#!l"s@ F C!7u!no@ E !n% Fo)l&!no@ V .00 Ann N1 A6!% S6& //. /00

.0 No:o*(m"s .00 No:o*(m"s !n% DSM l!un6 !6#(l!m&%" +&)$"#s C"m&6!l !n%

En)&n""#&n) N";s 5 .0./

./ O#$un .0/. L!s !s7!#!)&n!s!s %" m&6#oo#)!n&smos ( su uso

6l'n&6o BEB / /5

.. P!#H@ 1 K@ A-u6o;sH&@ A@ D!:&s@ S !n% D!:&s@ F // P!#m!6olo)( o+

E"#&6&! 6ol&L!s7!#!)&n!s" 7ol("$(l"n" )l(6ol !%%u6$ An$&6!n6"# R"s 22


15/21

15

.2 P!:"ss& A@ E V =.0/4? Es$&m!$" o+ A6#(l!m&%" In$!H" +#om Co++"" !n% J"!l$ R&sH

Ass"ssm"n$ In V P#""%(@ Co++"" &n J"!l$ !n% D&s"!s" P#":"n$&on@ 77 5554

N"; 1o#H A6!%"m&6 P#"ss

.4 P"%#"s6&@ F K@ K!!6H@ K G#!n-( .00 T" "++"6$ o+ !s7!#!)&n!s" on !6#(l!m&%"

+o#m!$&on &n F#"n6 +#&"s Foo% C"m&s$#(@ /0@ 77 22.

.5 P"$"#son@ RE C&")l"#@ A / LAs7!#!)&n!s" P#o%u6$&on -( V!#&ous B!6$"#&!

A77l&"% M&6#o-&olo)( / .20

. R"(nol%s DR@ T!(lo# , /2 T" Fun)!l Jolomo#7 A Cons&%"#!$&on o+ M&$o$&6

M"&o$&6 !n% Pl"omo#7&6 S7"6&!$&on@ CAB In$"#n!$&on!l@ !ll&n)+o#%@ UK

. Ro-"#$s@ ,@ ,M J&ll =/? Patente n!" #$1$%#& A. !".

".S6#"( D@ S7"&$"l K@ L!n:"#sK!m&nsH( C@ G"#ss ,@ Mo#u6H" A@ Boos , F&:" 1"!#C"n$"# S$u%( o+ !s7#!)&n!s" $"#!7( ;&$&n $" ALLBFM .000 $#&!l P"%&!$#&6

Bloo% C!n6"# .0//524

. S&n!@ R JR@ S&n) !n% SK@ ,! .0/2 M&6#o-&!l LAs7!#!)&n!s! P#"s"n$ !n%

+u$u#" P#os7"6$&:" I,IRSET . 02/05/

20 T!#"H" E@ R(%-"#) P@ K!#lsson P@ E#&Hsson S@ TY#n8:&s$ M An!l(s&s o+ !6#(l!m&%"@

! 6!#6&no)"n +o#m"% &n "!$"% +oo%s$u++s , A)#&6 Foo% C"m .00.504500

2/ V!n)@ J K@ Pu%"# A@ Gol%-"l6 .0/4 P!$"n$" n O .0/4//25 A/


16/21

16

2. V"#m!@ N K@ Kum!# G@ K!u# !n% S An!n% .00 LAs7!#!)&n!s" A P#om&s&n)

C"mo$"#!7"u$&6 A)"n$ C#&$&6!l R":&";s &n B&o$"6nolo)(@ . 45.

22 !n) B@ R"ll&n) MV@ S$o#m MC@ oo MJ@ R&-"o R@ Pu& CJ@ J!H L, E:!lu!$&on o+

&mmunolo)&6 6#oss #"!6$&on o+ !s$&!s7!#!)&n!s" !n$&-o%&"s &n !6u$" l(m7o-l!s$&6

l"uH"m&! =ALL? !n% l(m7om! 7!$&"n$s L"uH"m&! .002//52

BIBLIOGRAFIA

Eu#o7"!n Comm&ss&on COMMISSION RECOMMENDATION o+ /0/.0// on

&n:"s$&)!$&ons &n$o $" l":"ls o+ !6#(l!m&%" &n +oo% A:!&l!-l" on

$$7[["6"u#o7!"u[+oo%[+oo%[6"m&6!ls!+"$([6on$!m&n!n$s[#"6omm"n%!$&on^/00/.0//^!

6#(l!m&%"^+oo%^"n7%+ =on l&n"@ A66"ss"% .0/5 M!# .5?

US Foo% !n% D#u) A%m&n&s$#!$&on A6#(l!m&%"@ Fu#!n@ !n% $" FDA A:!&l!-l"

on$$7[[;;;+%!)o:[%o;nlo!%s[Foo%[Foo%-o#n"Illn"ssCon$!m&n!n$s[C"m&6!lCon$!m&n

!n$s[UCM/4427%+ =Onl&n"@ A66"ss"% .0/5 M!# .5?

/ #":&s$!s %" 25 #"+"#"n6&!s4@5 Z 0


17/21

17

ANE3OS

ANE3O /

MECANISMO DE ACCION ASPARAGINASA

F&)u#! / Es8u"m! m"6!n&smo %" #"!66&9n !s7!#!)&n!s!

Fu"n$" N""l!m V"#m!@ Kul%""7 Kum!# !n% Gu#noo# K!u#@ Sn" An!n% LAs7!#!)&n!s"

A P#om&s&n) C"mo$"#!7"u$&6 A)"n$


18/21

1

ANE3O .

DIFERENTES FUENTES BACTERIANAS PARA ASPARAGINASA


19/21

1!

T!-l! / D&+"#"n$"s Fu"n$"s -!6$"#&!n!s 7!#! !s7!#!)&n!s!Fu"n$" REP"$"#son !n% AC&")l"# L!s7!#!)&n!s" P#o%u6$&on -( V!#&ous B!6$"#&!


20/21

"0

ANE3O 2

EVOLUCION TEMPORAL DE LA APARACION DE ASPARAGINASA II

F&)u#! . E:olu6&9n $"m7o#!l %" l! !7!#&6&9n %" !s7!#!)&n!s! II "n 6ul$&:os

Fu"n$" Jo;!#% C"%!# !n% ,!m"s J S6;!#$* P#o%u6$&on o+ LAs7!#!)&n!s" II -(

Es6"#&6&! Col&

ANE3O 4


21/21

"1

USO INDUSTRIAL DE ACR1LAA1 EN EL MUNDO

F&)u#! 2 Uso &n%us$#&!l %" No:o*(m"s A6#(l!;!( "n "l mun%o !l !

Documents

Producción Asparaginasa