Probiotics in fish culture: immunomodulatory

and ecophysiological responses● 期刊 :Fish Physiol Biochem● DOI 10.1007/s10695-013-9897-0

第八組 :

組員 :4a1h0013 薛瑋均 4a1h0101 郭筱茹 4a1h0016 黃造培

AbstractAquaculture is emerging as one of the most viable

and promising enterprises for keeping pace with the surging need for animal protein providing nutritional and food security to humans,particularly those residing in regions where livestock is relatively scarce.

AbstractConventionally, the disease control in aquaculture has

relied on the use of chemical compounds and antibiotics.

The development of non-antibiotic and environmentally friendly agents is one of the key factors for health management in aquaculture.

IntroductionWhat is Probiotics ?

A bacterial supplement of a single or mixed culture of selected

non-pathogenic bacterial strains is termed probiotics.

Probiotics as ‘a live microbial feed supplement which

beneficially affects the host animal by improving its

intestinal microbial balance

Currently used in aquaculture probiotics, its main features

include the following:1. Increase nutrition and improve feed digestibility

Fish gut produces digestive enzymes with specific microorganisms can enhance digestion and absorption of feed may also be of intestinal sources of vitamins and essential amino acids

Currently used in aquaculture probiotics, its main features

include the following2.Irritating or cellular immunity

Some probiotics can stimulate aquatic organisms nonspecific immunity (nonspecific resistance)

ex:桿菌（ Bacillus sp.）可激活草蝦的免疫系統3.antiviral

Currently the antiviral mechanism of probiotics is not clear, but many studies have shown that probiotic substances or extracts produced virus can cause passivation

ex:在鮭魚卵巢分離出的  Pseudomonas sp.、 Vibrio sp.、 Aeromonas sp. 及 Corynebacterium sp.

Currently used in aquaculture probiotics, its main features

include the following4.Improve water quality

As the main features of probiotic bacteria because bacteria can affect the composition of the class of microorganisms in water and reduce the number of pathogens, and can improve water quality. The principle is the Gram-positive bacteria can be effectively converted to carbon dioxide organics

Common probiotic introduction

枯草桿菌 (Bacillus subtilis)Single cell 0.7 ~ 0.8 × 2 ~ 3 m. No capsule, Zhou flagella,

are Gram-positive bacteria. Is a kind of aerobic bacteria.

特性：1 . Can withstand temperatures of -60 to +280 ℃ ℃2 . Breed quickly, 4 to 6 hours to 10 times proliferation

3 . Absorbent containing gum, moisture and strong

4 . Organic matter decomposition and strong, able to break down large molecules into smaller molecules.

Common probiotic introduction

5 . Metabolites enzymes, organic acids, very rich

6 . Alkali resistance, high gas and low gas has strong antibacterial antagonism.

7 . Can have the stench of decomposing organic sulfur compounds, organic nitrogen, can deodorize

Common probiotic introduction單一枯草桿菌直接應用在水產養殖上，是無法發揮幫助水質轉化效果，因為枯草桿菌過於強勢，常會使水質變清而產生養殖上的困擾。一般枯草桿菌都和光合菌一起使用。

例如：三合一光合成菌是以光合成菌，枯草芽孢桿菌和硝化菌為主的菌種，還包含乳酸菌、酵母菌等。能在短期內降解氨，亞硝酸，硫化氫的功效非常明顯。

Common probiotic introduction功效：

1 .有害細菌具有很強的抑制作用。可防止腸炎、爛鰓等的疾病2 .能分解殘留的飼料和魚蝦的糞便。3 .可提高飼料中的蛋白質、脂肪酸、澱粉等等的活性，更有利於水產的吸收。4 .可以促進水產動物免疫器官的生長發育。能激活 T 、 B 淋巴細胞，提高水產動物的免疫球蛋白和抗體。

Common probiotic introduction光合菌 (Photosynthetic bacteria)

1 .光合細菌是一種營養豐富、價值高的細菌，菌體蛋白質含量高，是良好蛋白質來源。粗蛋白含量達到 60.22 ％，粗脂肪含量 8.19 ％，並含有豐富的胺基酸、葉酸、維生素 B群，尤其是維生素B12、類胡蘿蔔素和生物素。

2 .光合細菌對各種有機物質、氨、胺、硫化氫等有極強的利用能力，能分解亞硝酸 , 硝酸 , 增加水中溶氧量，有效的降低有毒物質的濃度。

Common probiotic introduction

4 .光合細菌個體小，營養豐富，可以做為浮游生物（例如輪蟲、橈足類、豐年蝦等）的餌料。而浮游生物又是水產生物 (如石斑魚苗等 )幼體的開口餌料，因此在食物鏈中有非常好的營養轉移傳遞作用。5 .當水中有機碳、氮太多時容易引發不良藻類如渦鞭毛藻、裸藻等爆發生長，光合菌可以阻斷或延緩該過程 ( 但光合菌太多相對的也會影響優良藻類的生長 )。光合菌更能減少魚肉的臭土味，而且增加魚體體色鮮豔程度和減少排泄的臭味。

Nitrifying bacteria growth environment1. Temperature ：  Nitrifying bacteria suitable temperature of

about 20 to 28 degrees c, the optimal growth temperature of about 25 degrees and reproduction c. General nitrifying bacteria at temperatures below 5 degrees c and above 42 degrees c to stop metabolism

2. Light ：  Unlike the nitrifying bacteria and certain proprietary green photosynthetic bacteria generally have photosynthetic pigments, and therefore can not take advantage of the sun for photosynthesis in organic synthesis. Not only can not take advantage of the sun, but? Afraid of sunlight

3. Substrate ：  Nitrifying bacteria much needed sediment, but the difference is that it's not intended for food, but sediment can provide attachment, shelter and access to a source of ammonia and nutrient sources they need. Not for breeding, and can not benefit ammonia source and a nutrient source for many of nitrifying bacteria is not found before the end of the appropriate quality.

Nitrifying bacteria growth environment

4. Water ： Since the characteristics of sessile nitrifying bacteria living resources must rely on water transport oxygen, ammonia and nutrients, such as it needs.

5. DO ： Oxygen is an essential element of life nitrifying bacteria. Advice is not less than 2ppm.

6. Ph value ： Generally, most prefer nitrifying bacteria grow in weakly alkaline in the ring, the ph value of about 7.5 to 8.2. ph value will affect the growth and reproduction of nitrifying bacteria. Nitrite bacteria ph range of 7.0 to 8.0, the optimal average of 7.8. Nitric bacteria from about 6.5 to 8.5.

Nitrification system to establish instances

Day 1~7Buy two or three more resistant to raising fish, feed a small amount

of feed. Next day to start measuring the concentration of ammonia. Concentrations will continue to rise for several days

Day 7~14About a week or so, because the nitrifying bacteria grow into,

began to enter the medium term. Concentration of ammonia will quickly subside. During this period, if the fish in poor condition, a small amount of water changes to dilute the tank of water. Relative, nitrite concentration started to climb a week later, and ammonia test synchronization, start testing nitrite. amount once every two days, nitrite will reach the peak and then decline slowly

Nitrification system to establish instances

Day 17Began in the later stages. Another one week, when nitrite and

ammonia concentrations are reduced to zero, nitrate concentrations began to increase this time, congratulations greatly. Systems created. Additional fish do not rush. First change a small amount of water. then wait two days plus fish. than once and do not add too much. otherwise add too much at once, the system will collapse. efforts made three weeks ago on the East into water.

Nitrifying bacteria growing1. Slow period ： When new to nitrifying bacteria living

substances, growth lines to adapt to the new environment without increasing the number of periods of nitrifying bacteria. The length of this period, depending on the extent of the impact the new environment of nitrifying bacteria may be.

2. Logarithmic growth phase ： After the bacteria adapt to the new environment, rapid oxidation of ammonia or nitrite to obtain a fixed period of inorganic carbon energy, thus accelerating the reaction of organic synthesis, the number of surge is the logarithmic growth phase, the growth of the cover into the case-based progression increases. At this stage, the reproductive rate of up to the maximum.

Nitrifying bacteria growing3. Decreasing Growing ： When ammonia or nitrite

concentration gradually reduced to a point where the nitrifying bacteria, the remaining resources will limit the growth rate of growth of nitrifying bacteria, so increasing the number of nitrifying bacteria, it becomes very slow period.

4. Resting ： Nitrifying bacteria grow in the environment is limited impact factor (such as insufficient source of ammonia) has reached saturation, where growth rate is equal to the death rate has been the number of nitrifying bacteria to reach a stable state. Due to the relatively short resting, so it was omitted to distinguish this period.

Nitrifying bacteria growing5. The breath of ：

Also known as endogenous period. Ammonia or nitrite in the system have been exhausted, the nitrifying bacteria was "hungry" state, only to continue the use of in vivo metabolism of nutrients, the death rate is greatly increased, greatly reducing the number of periods.

硝化系統簡易圖

Conclusion● Characteristics of probiotics in aquaculture

applications include: improved nutrition and increased feed digestibility, stimulate or enhance cellular immunity, thus competing with pathogenic bacteria exclusion, improve water quality and resistance to viruses and other probiotics, but now most of its mechanism of action is still is not clear, so the selection of probiotics, it is necessary to determine the host harmless.

Thank you