Printed functionality From sensor platforms towards cell arrays

Jouko Peltonen Laboratory of physical chemistry

FunMat I (2008) - Objectives

9.10.2009

1. Modification and control of the properties of

natural fiber-based substrates in order to make them compatible with the added functionality concepts developed by the other partners of CoE

2. Control of the processability of functional (raw) materials as defined by the surface treatment, coating or printing processes that are used to assemble the novel functional surfaces, sensors and devices; printability, print quality

Roll-to-roll fabrication of reaction arrays

Määttänen et al. Sensors and Actuators B 160 (2011), 1401-1412

1. Develop an ink formulation of a hydrophobic polymer (PDMS)

2. Design a print pattern 3. Print & cure 4. A reaction array for

applications

Paper as a sensor platform

Screening assays cells, biofilms, pharmaceutical ingridients

Colorimetric indicators biomaterials, hazardous gases, EC pixels Electrochemical sensors

glucose, pH

Chemoresistors hazardous gases. humidity

Fluidics Liquid transport and filtration

A paper-based assay platform

Staphylococcus aureus biofilm

Retinal pigment epitheal cells

• Määttänen, Ihalainen, Pulkkinen, Wang, Tenhu, Peltonen, Applied Materials & Interfaces 4 (2012), 955-964.

• Sarfraz, Ihalainen, Määttänen, Bollström, Gulin, Peltonen, Lindén, Colloids and Surfaces A 460 (2014), 401-407.

Member Professor Jouko Peltonen, Lab. Physical Chemistry (DPC) Member Professor Carl-Eric Wilén, Lab. Polymer Technology (LPT) Co-Chair Professor John Eriksson, Lab. Cell Biology (LCB) Member Professor Martti Toivakka, Lab. Paper Coating and Converting (LPCC) Chair Professor Ronald Österbacka, Department of Physics (DPh)

Center of Excellence in

Functional Materials at Biological Interfaces .

http://www.funmat.fi

Åbo Akademi University Center of Excellence 2015-

FunMat II - Objectives

Functionalized materials enable controlled interfaces with cells Develop materials and surfaces with controlled

physico-chemical properties to clarify and control materials interaction with living cells

Functional materials provide new approaches for sensing biological processes Develop biointerfaces for real-time monitoring of

the cell-response to follow the substrate-cell interaction in a controlled way

Environment vs. cell fate

Fig. 3. Schematic description of how various substrate parameters will influence the cell‐substrate interaction leading to proliferation, migration and differentiation. Figure adapted from Murphy et al., Nature Materials (2014).

FunMat II

A paper-based assay platform for protein and cell studies

FunMat II

P. Ihalainen, A. Määttänen, J. Järnström, D. Tobjörk, R. Österbacka, J. Peltonen, Industrial and Engineering Chemistry Research 51 (2012), 6025−6036.

• Cell growth was supported by surfaces with large bearing area and low surface area ratio.

• High total surface free energy and an intermediate electron donor surface energy component were preferential for cell growth.

ARPE-19 cells cultured on the reaction areas on various paper substrates (P-2, P-3, P-4) after 24, 48 and 72 h of incubation. The scale bar is 0.5 mm.

Image size: 1 µm x 1 µm BSA = Bovine serum albumin (66.5 kDa) FN = Fibronectin (440 kDa) SA = Streptavidin (60 kDa) P

ristin

e

BSA FN SA

IR s

inte

red

• Proteins adhered preferentially on the hydrophobic regions of the surface

• Proteins adhered better on the more negatively charged latex component.

• Protein adsorption affected the surface (contact) potential of the surface.

Assay development

FunMat II

FET

Prin

ted

devi

ces

for d

etec

tion/

sens

ing,

an

d co

ntro

lled/

prog

ram

mab

le e

lect

ro-

chem

ical

stim

uli a

nd c

hem

ical

rele

ase.

Printed, coated and patterned multi-parameter paper-based cell-growth array with controlled physico-chemical properties.

H. Juvonen, A. Määttänen, P. Ihalainen, T. Viitala, J. Sarfraz, J. Peltonen, Colloids Surfaces B 118 (2014), 261-269.

Active regulation of cell fate

FunMat II

Thank you !

www.funmat.fi