principe du "co-patching" (résolution : ~ 50 nm)

rassemblement séparé

récepteurs associés

récepteurs séparés

rassemblement coordonné

+ anticorps

+ anticorps

Double transfected cell

Selected field in the two-channels

Gray images - contrast optimalization

Binary image / Erode / Dilate / Watershed filter

BLUE

Analysis of the fields

Results of patching

• Patches in red : 189• Patches in green: 155• Patches red and green :153

• Overlap : 2 x Patches(R+G) / Patches(R) + Patches(G)

• Result for this field = 2 x 153 / 189 + 155 = 0.889

• 89 % co-patching